Streptococcus satellite phage Javan413

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4D5ZM60|A0A4D5ZM60_9VIRU Integrase OS=Streptococcus satellite phage Javan413 OX=2558689 GN=JavanS413_0012 PE=3 SV=1
MM1 pKa = 7.2KK2 pKa = 9.64TLSDD6 pKa = 3.59TPKK9 pKa = 9.76TFTFTTTLPNFEE21 pKa = 4.31QAQKK25 pKa = 10.83AGSALIGYY33 pKa = 7.54MIGTYY38 pKa = 9.63DD39 pKa = 3.89QSAIDD44 pKa = 3.22ITYY47 pKa = 9.12TGNGNLKK54 pKa = 10.56AEE56 pKa = 4.29YY57 pKa = 9.75TSDD60 pKa = 3.51EE61 pKa = 4.37DD62 pKa = 3.52LTEE65 pKa = 4.1SFEE68 pKa = 5.63RR69 pKa = 11.84ITNSLNHH76 pKa = 6.52TDD78 pKa = 4.41DD79 pKa = 4.37FEE81 pKa = 6.99DD82 pKa = 4.69YY83 pKa = 11.06EE84 pKa = 6.23DD85 pKa = 5.59DD86 pKa = 6.03FEE88 pKa = 8.21DD89 pKa = 5.79DD90 pKa = 4.55DD91 pKa = 4.4LTIKK95 pKa = 10.86GDD97 pKa = 3.82LANYY101 pKa = 6.99TALIGSFNTLGEE113 pKa = 4.17AQAFTEE119 pKa = 4.58NLDD122 pKa = 4.44DD123 pKa = 6.72DD124 pKa = 4.55LTNGNNFVYY133 pKa = 9.96EE134 pKa = 4.1QTPDD138 pKa = 3.27TVALYY143 pKa = 9.58VSPQEE148 pKa = 4.29TIANSTIQKK157 pKa = 10.38LEE159 pKa = 3.71EE160 pKa = 4.37AYY162 pKa = 10.58VKK164 pKa = 10.75HH165 pKa = 5.76EE166 pKa = 4.46ADD168 pKa = 3.79YY169 pKa = 11.46EE170 pKa = 4.28PDD172 pKa = 3.54TFKK175 pKa = 11.14LSYY178 pKa = 10.7HH179 pKa = 6.34DD180 pKa = 4.09MRR182 pKa = 11.84VQQLQSLDD190 pKa = 3.73KK191 pKa = 10.18DD192 pKa = 3.95TLISDD197 pKa = 4.3IILYY201 pKa = 9.87EE202 pKa = 4.7LEE204 pKa = 4.7LLDD207 pKa = 4.53HH208 pKa = 7.54ADD210 pKa = 3.89RR211 pKa = 11.84LLSDD215 pKa = 4.22EE216 pKa = 5.14PLPLDD221 pKa = 4.17DD222 pKa = 3.37QHH224 pKa = 8.21GFEE227 pKa = 4.67TIEE230 pKa = 4.04LLGDD234 pKa = 3.73DD235 pKa = 4.96VIDD238 pKa = 4.37LVKK241 pKa = 10.75EE242 pKa = 3.9LDD244 pKa = 3.4TSKK247 pKa = 10.86EE248 pKa = 3.45FDD250 pKa = 4.59GIHH253 pKa = 7.29DD254 pKa = 4.33YY255 pKa = 11.0IIGGG259 pKa = 3.65

Molecular weight:
29.21 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4D5ZM77|A0A4D5ZM77_9VIRU HTH cro/C1-type domain-containing protein OS=Streptococcus satellite phage Javan413 OX=2558689 GN=JavanS413_0010 PE=4 SV=1
MM1 pKa = 7.83KK2 pKa = 10.07IIEE5 pKa = 4.24YY6 pKa = 9.85KK7 pKa = 10.36KK8 pKa = 10.26KK9 pKa = 10.46NGTIMYY15 pKa = 9.39RR16 pKa = 11.84SQIYY20 pKa = 10.54LGVDD24 pKa = 3.78SITGKK29 pKa = 10.33KK30 pKa = 10.19ARR32 pKa = 11.84TSVSGRR38 pKa = 11.84TKK40 pKa = 10.78KK41 pKa = 9.89EE42 pKa = 3.5LKK44 pKa = 10.87NNIKK48 pKa = 9.59LAKK51 pKa = 10.46YY52 pKa = 8.03EE53 pKa = 4.07FQANGEE59 pKa = 4.46TVSKK63 pKa = 10.21KK64 pKa = 9.68VQVKK68 pKa = 8.92TYY70 pKa = 9.61SQLVEE75 pKa = 4.2LWLEE79 pKa = 4.11NYY81 pKa = 10.23KK82 pKa = 9.32LTIKK86 pKa = 9.75PQTYY90 pKa = 9.3RR91 pKa = 11.84VTTRR95 pKa = 11.84LIDD98 pKa = 3.71CHH100 pKa = 6.68LLPYY104 pKa = 9.23FGKK107 pKa = 9.86MKK109 pKa = 10.23VDD111 pKa = 4.28KK112 pKa = 10.75ISNTDD117 pKa = 2.69IQSFVNGLSSKK128 pKa = 10.17LVNFRR133 pKa = 11.84TVNSINTRR141 pKa = 11.84VLQYY145 pKa = 10.84GVSLNLIPFNPARR158 pKa = 11.84DD159 pKa = 3.53VILPKK164 pKa = 9.97KK165 pKa = 9.65QKK167 pKa = 9.89PGRR170 pKa = 11.84DD171 pKa = 3.08SVKK174 pKa = 10.81FIDD177 pKa = 3.97TNDD180 pKa = 3.44LKK182 pKa = 10.45TLLNFMEE189 pKa = 4.37KK190 pKa = 10.19QSFRR194 pKa = 11.84RR195 pKa = 11.84YY196 pKa = 8.64RR197 pKa = 11.84YY198 pKa = 7.54QVEE201 pKa = 4.13YY202 pKa = 11.1VFYY205 pKa = 10.82KK206 pKa = 10.89LLLATGLRR214 pKa = 11.84IGEE217 pKa = 4.13AVALEE222 pKa = 4.13WSDD225 pKa = 3.65IDD227 pKa = 5.53LEE229 pKa = 4.32NATIDD234 pKa = 3.43INKK237 pKa = 9.94SYY239 pKa = 10.46SQEE242 pKa = 3.67LRR244 pKa = 11.84LVSTTKK250 pKa = 10.14TKK252 pKa = 10.93AGTRR256 pKa = 11.84LISIDD261 pKa = 3.8KK262 pKa = 8.85EE263 pKa = 4.68TVNLLKK269 pKa = 10.53LYY271 pKa = 8.46QVRR274 pKa = 11.84QRR276 pKa = 11.84QLFHH280 pKa = 6.65EE281 pKa = 4.75VGGGVPKK288 pKa = 10.71VAFATPTRR296 pKa = 11.84EE297 pKa = 3.87YY298 pKa = 10.93LSRR301 pKa = 11.84LTLQNNLNSRR311 pKa = 11.84CEE313 pKa = 3.75RR314 pKa = 11.84LGIPRR319 pKa = 11.84FTFHH323 pKa = 7.74AFRR326 pKa = 11.84HH327 pKa = 4.69THH329 pKa = 6.92ASLLLNAGISYY340 pKa = 10.68KK341 pKa = 10.23EE342 pKa = 3.61LQYY345 pKa = 11.52RR346 pKa = 11.84LGHH349 pKa = 5.94SNIAMTLDD357 pKa = 3.93VYY359 pKa = 11.5SHH361 pKa = 7.06LSKK364 pKa = 11.04DD365 pKa = 3.51KK366 pKa = 10.41EE367 pKa = 4.25KK368 pKa = 10.93EE369 pKa = 3.85AVSYY373 pKa = 9.5YY374 pKa = 10.51EE375 pKa = 4.18KK376 pKa = 10.61AINSII381 pKa = 3.83

Molecular weight:
43.95 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

1886

42

381

157.2

18.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.56 ± 0.347

0.636 ± 0.166

6.787 ± 1.079

8.908 ± 1.073

3.552 ± 0.304

4.454 ± 0.39

1.166 ± 0.263

8.271 ± 0.515

9.491 ± 0.735

9.862 ± 0.617

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.227 ± 0.367

6.522 ± 0.48

2.28 ± 0.373

4.03 ± 0.369

4.719 ± 0.779

5.992 ± 0.525

6.363 ± 0.751

4.242 ± 0.629

0.954 ± 0.215

4.984 ± 0.489

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski