Bacteriophage APSE-3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Sendosyvirus; unclassified Sendosyvirus

Average proteome isoelectric point is 7.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 38 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6F9EWR1|A0A6F9EWR1_9CAUD VRR-NUC domain-containing protein OS=Bacteriophage APSE-3 OX=568989 GN=APPETEDU2671_10 PE=4 SV=1
MM1 pKa = 7.57TKK3 pKa = 10.19PLTKK7 pKa = 10.75GEE9 pKa = 4.11IVLFALRR16 pKa = 11.84KK17 pKa = 9.96AGIASEE23 pKa = 4.06ATNIDD28 pKa = 3.54VEE30 pKa = 4.33PQSFEE35 pKa = 3.96EE36 pKa = 5.28GINDD40 pKa = 5.03LEE42 pKa = 4.46DD43 pKa = 4.59LMAEE47 pKa = 4.07LQITFGDD54 pKa = 3.66LGYY57 pKa = 10.61QFSAEE62 pKa = 4.23EE63 pKa = 4.09EE64 pKa = 4.38NPTSDD69 pKa = 4.66DD70 pKa = 3.66ASGLPRR76 pKa = 11.84KK77 pKa = 9.08YY78 pKa = 9.79KK79 pKa = 10.43QVMGYY84 pKa = 9.4QLMLRR89 pKa = 11.84MLSDD93 pKa = 3.68YY94 pKa = 10.87GIEE97 pKa = 3.79PTPRR101 pKa = 11.84QEE103 pKa = 3.96ASAAAAYY110 pKa = 8.09DD111 pKa = 3.69ALLTDD116 pKa = 4.15TLSVPSIARR125 pKa = 11.84RR126 pKa = 11.84GDD128 pKa = 3.4MPVGQGNNYY137 pKa = 7.5TALGTASYY145 pKa = 9.24YY146 pKa = 9.37VEE148 pKa = 4.41RR149 pKa = 11.84GFHH152 pKa = 6.76AKK154 pKa = 8.23NTDD157 pKa = 3.4PVSS160 pKa = 3.22

Molecular weight: 17.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6F9EWK7|A0A6F9EWK7_9CAUD RHS-repeat-containing toxin OS=Bacteriophage APSE-3 OX=568989 GN=APPETEDU2671_25 PE=4 SV=1
MM1 pKa = 7.23HH2 pKa = 7.53WKK4 pKa = 10.35LLLTAGLLATISALAIVVRR23 pKa = 11.84FQYY26 pKa = 10.77VEE28 pKa = 3.86NVRR31 pKa = 11.84LKK33 pKa = 10.67LANQSLSAEE42 pKa = 3.99RR43 pKa = 11.84DD44 pKa = 3.06AARR47 pKa = 11.84AQFTHH52 pKa = 6.49YY53 pKa = 10.43AQAIDD58 pKa = 3.81IFNTIAGANQDD69 pKa = 3.25AHH71 pKa = 5.41QQAIQASQPQTIKK84 pKa = 9.97IQEE87 pKa = 4.65AITPEE92 pKa = 4.19RR93 pKa = 11.84CAHH96 pKa = 6.12LPVPTAAVNRR106 pKa = 11.84LRR108 pKa = 11.84AHH110 pKa = 7.02ADD112 pKa = 3.38KK113 pKa = 10.56ISPRR117 pKa = 11.84AASAHH122 pKa = 5.74SSNVTRR128 pKa = 5.04

Molecular weight: 13.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

38

0

38

12505

62

1621

329.1

36.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.972 ± 0.469

0.952 ± 0.138

5.83 ± 0.247

6.046 ± 0.316

3.247 ± 0.182

6.645 ± 0.347

2.047 ± 0.164

5.918 ± 0.255

6.789 ± 0.271

8.501 ± 0.336

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.351 ± 0.232

5.078 ± 0.28

4.15 ± 0.183

4.718 ± 0.339

5.446 ± 0.274

6.565 ± 0.294

6.325 ± 0.533

5.614 ± 0.255

1.511 ± 0.192

3.295 ± 0.273
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski