Amino acid dipepetide frequency for Bacteriophage APSE-3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.598AlaAla: 7.598 ± 1.381
0.72AlaCys: 0.72 ± 0.327
5.518AlaAsp: 5.518 ± 0.766
6.478AlaGlu: 6.478 ± 0.897
3.119AlaPhe: 3.119 ± 0.484
5.998AlaGly: 5.998 ± 0.73
1.999AlaHis: 1.999 ± 0.463
4.639AlaIle: 4.639 ± 0.599
6.798AlaLys: 6.798 ± 0.785
10.317AlaLeu: 10.317 ± 1.222
2.239AlaMet: 2.239 ± 0.593
4.079AlaAsn: 4.079 ± 0.714
2.799AlaPro: 2.799 ± 0.518
4.399AlaGln: 4.399 ± 0.731
3.439AlaArg: 3.439 ± 0.62
5.518AlaSer: 5.518 ± 0.794
4.639AlaThr: 4.639 ± 0.492
4.639AlaVal: 4.639 ± 0.628
1.759AlaTrp: 1.759 ± 0.384
3.039AlaTyr: 3.039 ± 0.582
0.0AlaXaa: 0.0 ± 0.0
Cys
0.48CysAla: 0.48 ± 0.194
0.24CysCys: 0.24 ± 0.142
0.64CysAsp: 0.64 ± 0.28
0.08CysGlu: 0.08 ± 0.07
0.88CysPhe: 0.88 ± 0.304
0.72CysGly: 0.72 ± 0.225
0.08CysHis: 0.08 ± 0.082
0.24CysIle: 0.24 ± 0.132
0.72CysLys: 0.72 ± 0.273
1.52CysLeu: 1.52 ± 0.346
0.4CysMet: 0.4 ± 0.207
0.32CysAsn: 0.32 ± 0.164
0.56CysPro: 0.56 ± 0.205
0.4CysGln: 0.4 ± 0.17
0.72CysArg: 0.72 ± 0.27
0.32CysSer: 0.32 ± 0.139
0.48CysThr: 0.48 ± 0.189
0.32CysVal: 0.32 ± 0.158
0.08CysTrp: 0.08 ± 0.089
0.32CysTyr: 0.32 ± 0.16
0.0CysXaa: 0.0 ± 0.0
Asp
5.438AspAla: 5.438 ± 0.723
0.4AspCys: 0.4 ± 0.158
3.519AspAsp: 3.519 ± 0.494
4.559AspGlu: 4.559 ± 0.571
1.52AspPhe: 1.52 ± 0.52
4.559AspGly: 4.559 ± 0.496
0.48AspHis: 0.48 ± 0.191
3.759AspIle: 3.759 ± 0.53
4.718AspLys: 4.718 ± 0.68
4.639AspLeu: 4.639 ± 0.824
1.679AspMet: 1.679 ± 0.498
3.359AspAsn: 3.359 ± 0.516
3.119AspPro: 3.119 ± 0.558
1.28AspGln: 1.28 ± 0.377
1.919AspArg: 1.919 ± 0.347
3.679AspSer: 3.679 ± 0.64
3.279AspThr: 3.279 ± 0.54
3.759AspVal: 3.759 ± 0.655
0.8AspTrp: 0.8 ± 0.258
1.839AspTyr: 1.839 ± 0.368
0.0AspXaa: 0.0 ± 0.0
Glu
6.238GluAla: 6.238 ± 0.942
0.48GluCys: 0.48 ± 0.178
2.239GluAsp: 2.239 ± 0.482
3.359GluGlu: 3.359 ± 0.706
1.999GluPhe: 1.999 ± 0.464
3.439GluGly: 3.439 ± 0.474
1.04GluHis: 1.04 ± 0.297
4.079GluIle: 4.079 ± 0.525
5.198GluLys: 5.198 ± 0.789
5.678GluLeu: 5.678 ± 0.535
1.44GluMet: 1.44 ± 0.469
3.119GluAsn: 3.119 ± 0.516
1.999GluPro: 1.999 ± 0.546
3.039GluGln: 3.039 ± 0.496
3.279GluArg: 3.279 ± 0.575
3.199GluSer: 3.199 ± 0.46
3.679GluThr: 3.679 ± 0.517
3.919GluVal: 3.919 ± 0.615
0.88GluTrp: 0.88 ± 0.304
2.079GluTyr: 2.079 ± 0.435
0.0GluXaa: 0.0 ± 0.0
Phe
2.959PheAla: 2.959 ± 0.612
0.8PheCys: 0.8 ± 0.25
3.359PheAsp: 3.359 ± 0.455
1.759PheGlu: 1.759 ± 0.421
0.72PhePhe: 0.72 ± 0.337
2.479PheGly: 2.479 ± 0.423
0.4PheHis: 0.4 ± 0.18
1.679PheIle: 1.679 ± 0.379
2.079PheLys: 2.079 ± 0.354
1.759PheLeu: 1.759 ± 0.303
0.72PheMet: 0.72 ± 0.301
1.999PheAsn: 1.999 ± 0.388
1.36PhePro: 1.36 ± 0.378
0.96PheGln: 0.96 ± 0.324
1.839PheArg: 1.839 ± 0.455
2.319PheSer: 2.319 ± 0.49
2.399PheThr: 2.399 ± 0.491
1.599PheVal: 1.599 ± 0.42
0.32PheTrp: 0.32 ± 0.128
0.96PheTyr: 0.96 ± 0.348
0.0PheXaa: 0.0 ± 0.0
Gly
5.038GlyAla: 5.038 ± 0.864
0.56GlyCys: 0.56 ± 0.218
3.759GlyAsp: 3.759 ± 0.693
4.079GlyGlu: 4.079 ± 0.718
3.119GlyPhe: 3.119 ± 0.431
4.798GlyGly: 4.798 ± 1.036
0.96GlyHis: 0.96 ± 0.297
5.758GlyIle: 5.758 ± 0.731
4.958GlyLys: 4.958 ± 0.445
5.518GlyLeu: 5.518 ± 0.715
1.36GlyMet: 1.36 ± 0.352
3.039GlyAsn: 3.039 ± 0.738
1.04GlyPro: 1.04 ± 0.312
3.519GlyGln: 3.519 ± 0.707
4.079GlyArg: 4.079 ± 0.912
4.239GlySer: 4.239 ± 0.624
4.159GlyThr: 4.159 ± 0.491
3.599GlyVal: 3.599 ± 0.545
0.88GlyTrp: 0.88 ± 0.229
1.999GlyTyr: 1.999 ± 0.383
0.0GlyXaa: 0.0 ± 0.0
His
1.999HisAla: 1.999 ± 0.324
0.0HisCys: 0.0 ± 0.0
1.36HisAsp: 1.36 ± 0.333
1.12HisGlu: 1.12 ± 0.328
0.96HisPhe: 0.96 ± 0.265
0.96HisGly: 0.96 ± 0.31
0.56HisHis: 0.56 ± 0.171
1.04HisIle: 1.04 ± 0.259
1.12HisLys: 1.12 ± 0.267
2.399HisLeu: 2.399 ± 0.536
0.32HisMet: 0.32 ± 0.117
0.96HisAsn: 0.96 ± 0.269
1.04HisPro: 1.04 ± 0.279
1.44HisGln: 1.44 ± 0.39
1.2HisArg: 1.2 ± 0.303
1.28HisSer: 1.28 ± 0.291
1.04HisThr: 1.04 ± 0.429
0.88HisVal: 0.88 ± 0.229
0.56HisTrp: 0.56 ± 0.222
0.24HisTyr: 0.24 ± 0.136
0.0HisXaa: 0.0 ± 0.0
Ile
6.078IleAla: 6.078 ± 0.706
0.72IleCys: 0.72 ± 0.278
3.839IleAsp: 3.839 ± 0.646
5.438IleGlu: 5.438 ± 0.819
1.36IlePhe: 1.36 ± 0.315
2.959IleGly: 2.959 ± 0.594
1.12IleHis: 1.12 ± 0.352
3.279IleIle: 3.279 ± 0.695
4.079IleLys: 4.079 ± 0.691
3.039IleLeu: 3.039 ± 0.475
0.8IleMet: 0.8 ± 0.248
3.759IleAsn: 3.759 ± 0.475
2.719IlePro: 2.719 ± 0.422
2.639IleGln: 2.639 ± 0.539
2.559IleArg: 2.559 ± 0.486
4.639IleSer: 4.639 ± 0.565
4.639IleThr: 4.639 ± 0.764
3.039IleVal: 3.039 ± 0.438
0.64IleTrp: 0.64 ± 0.194
1.839IleTyr: 1.839 ± 0.315
0.0IleXaa: 0.0 ± 0.0
Lys
6.718LysAla: 6.718 ± 0.694
0.64LysCys: 0.64 ± 0.251
3.759LysAsp: 3.759 ± 0.607
3.599LysGlu: 3.599 ± 0.474
1.28LysPhe: 1.28 ± 0.393
3.759LysGly: 3.759 ± 0.557
1.28LysHis: 1.28 ± 0.195
3.759LysIle: 3.759 ± 0.599
4.159LysLys: 4.159 ± 0.525
5.598LysLeu: 5.598 ± 0.686
2.479LysMet: 2.479 ± 0.473
3.359LysAsn: 3.359 ± 0.57
2.959LysPro: 2.959 ± 0.676
3.199LysGln: 3.199 ± 0.455
5.198LysArg: 5.198 ± 0.819
5.038LysSer: 5.038 ± 0.678
4.878LysThr: 4.878 ± 0.644
2.959LysVal: 2.959 ± 0.499
1.36LysTrp: 1.36 ± 0.347
1.919LysTyr: 1.919 ± 0.401
0.0LysXaa: 0.0 ± 0.0
Leu
8.237LeuAla: 8.237 ± 0.75
1.12LeuCys: 1.12 ± 0.267
3.359LeuAsp: 3.359 ± 0.494
4.319LeuGlu: 4.319 ± 0.582
2.479LeuPhe: 2.479 ± 0.424
5.918LeuGly: 5.918 ± 0.935
1.28LeuHis: 1.28 ± 0.268
5.118LeuIle: 5.118 ± 0.6
5.278LeuLys: 5.278 ± 0.719
6.478LeuLeu: 6.478 ± 0.762
2.239LeuMet: 2.239 ± 0.472
4.319LeuAsn: 4.319 ± 0.485
4.798LeuPro: 4.798 ± 0.864
3.519LeuGln: 3.519 ± 0.563
5.038LeuArg: 5.038 ± 0.804
7.118LeuSer: 7.118 ± 0.613
6.878LeuThr: 6.878 ± 0.938
4.239LeuVal: 4.239 ± 0.605
0.96LeuTrp: 0.96 ± 0.312
2.319LeuTyr: 2.319 ± 0.494
0.0LeuXaa: 0.0 ± 0.0
Met
2.079MetAla: 2.079 ± 0.551
0.16MetCys: 0.16 ± 0.098
0.96MetAsp: 0.96 ± 0.255
1.2MetGlu: 1.2 ± 0.436
0.48MetPhe: 0.48 ± 0.174
1.839MetGly: 1.839 ± 0.341
0.32MetHis: 0.32 ± 0.159
0.88MetIle: 0.88 ± 0.262
1.679MetLys: 1.679 ± 0.495
2.239MetLeu: 2.239 ± 0.458
0.88MetMet: 0.88 ± 0.321
1.04MetAsn: 1.04 ± 0.26
1.04MetPro: 1.04 ± 0.383
1.36MetGln: 1.36 ± 0.404
1.759MetArg: 1.759 ± 0.401
1.759MetSer: 1.759 ± 0.52
1.599MetThr: 1.599 ± 0.343
1.28MetVal: 1.28 ± 0.306
0.32MetTrp: 0.32 ± 0.164
0.64MetTyr: 0.64 ± 0.246
0.0MetXaa: 0.0 ± 0.0
Asn
5.038AsnAla: 5.038 ± 0.783
0.64AsnCys: 0.64 ± 0.295
2.399AsnAsp: 2.399 ± 0.538
2.559AsnGlu: 2.559 ± 0.4
0.72AsnPhe: 0.72 ± 0.21
3.839AsnGly: 3.839 ± 0.64
1.839AsnHis: 1.839 ± 0.364
3.359AsnIle: 3.359 ± 0.394
3.039AsnLys: 3.039 ± 0.498
4.079AsnLeu: 4.079 ± 0.593
0.8AsnMet: 0.8 ± 0.254
2.879AsnAsn: 2.879 ± 0.542
2.399AsnPro: 2.399 ± 0.387
2.159AsnGln: 2.159 ± 0.559
3.519AsnArg: 3.519 ± 0.484
3.279AsnSer: 3.279 ± 0.451
3.359AsnThr: 3.359 ± 0.475
2.159AsnVal: 2.159 ± 0.457
0.72AsnTrp: 0.72 ± 0.244
1.999AsnTyr: 1.999 ± 0.526
0.0AsnXaa: 0.0 ± 0.0
Pro
2.879ProAla: 2.879 ± 0.467
0.16ProCys: 0.16 ± 0.097
3.119ProAsp: 3.119 ± 0.456
2.559ProGlu: 2.559 ± 0.496
1.04ProPhe: 1.04 ± 0.319
2.719ProGly: 2.719 ± 0.38
0.96ProHis: 0.96 ± 0.273
2.479ProIle: 2.479 ± 0.434
3.199ProLys: 3.199 ± 0.567
3.279ProLeu: 3.279 ± 0.407
0.72ProMet: 0.72 ± 0.281
2.239ProAsn: 2.239 ± 0.494
1.2ProPro: 1.2 ± 0.35
2.079ProGln: 2.079 ± 0.413
2.079ProArg: 2.079 ± 0.597
2.719ProSer: 2.719 ± 0.52
3.119ProThr: 3.119 ± 0.522
3.039ProVal: 3.039 ± 0.488
0.4ProTrp: 0.4 ± 0.19
1.52ProTyr: 1.52 ± 0.372
0.0ProXaa: 0.0 ± 0.0
Gln
3.999GlnAla: 3.999 ± 0.691
0.32GlnCys: 0.32 ± 0.155
2.239GlnAsp: 2.239 ± 0.374
2.639GlnGlu: 2.639 ± 0.671
2.239GlnPhe: 2.239 ± 0.481
2.399GlnGly: 2.399 ± 0.529
0.8GlnHis: 0.8 ± 0.262
3.519GlnIle: 3.519 ± 0.614
2.799GlnLys: 2.799 ± 0.39
4.159GlnLeu: 4.159 ± 0.531
1.12GlnMet: 1.12 ± 0.283
2.319GlnAsn: 2.319 ± 0.737
2.159GlnPro: 2.159 ± 0.425
3.119GlnGln: 3.119 ± 0.64
2.879GlnArg: 2.879 ± 0.463
2.719GlnSer: 2.719 ± 0.624
3.119GlnThr: 3.119 ± 0.613
2.319GlnVal: 2.319 ± 0.469
0.64GlnTrp: 0.64 ± 0.206
1.679GlnTyr: 1.679 ± 0.529
0.0GlnXaa: 0.0 ± 0.0
Arg
4.399ArgAla: 4.399 ± 0.538
0.32ArgCys: 0.32 ± 0.156
2.799ArgAsp: 2.799 ± 0.393
3.279ArgGlu: 3.279 ± 0.516
3.199ArgPhe: 3.199 ± 0.453
2.319ArgGly: 2.319 ± 0.39
1.28ArgHis: 1.28 ± 0.303
3.999ArgIle: 3.999 ± 0.601
4.079ArgLys: 4.079 ± 0.823
4.958ArgLeu: 4.958 ± 0.54
1.12ArgMet: 1.12 ± 0.253
3.439ArgAsn: 3.439 ± 0.545
1.919ArgPro: 1.919 ± 0.452
2.079ArgGln: 2.079 ± 0.372
3.199ArgArg: 3.199 ± 0.683
2.719ArgSer: 2.719 ± 0.45
2.399ArgThr: 2.399 ± 0.491
3.439ArgVal: 3.439 ± 0.513
1.52ArgTrp: 1.52 ± 0.391
1.999ArgTyr: 1.999 ± 0.54
0.0ArgXaa: 0.0 ± 0.0
Ser
5.758SerAla: 5.758 ± 0.526
0.72SerCys: 0.72 ± 0.266
4.159SerAsp: 4.159 ± 0.528
4.159SerGlu: 4.159 ± 0.535
2.239SerPhe: 2.239 ± 0.379
5.038SerGly: 5.038 ± 0.615
1.919SerHis: 1.919 ± 0.494
3.439SerIle: 3.439 ± 0.56
3.599SerLys: 3.599 ± 0.449
5.598SerLeu: 5.598 ± 0.847
1.999SerMet: 1.999 ± 0.382
3.039SerAsn: 3.039 ± 0.555
4.079SerPro: 4.079 ± 0.551
3.119SerGln: 3.119 ± 0.479
2.879SerArg: 2.879 ± 0.482
3.759SerSer: 3.759 ± 0.495
3.119SerThr: 3.119 ± 0.569
4.639SerVal: 4.639 ± 0.518
0.88SerTrp: 0.88 ± 0.24
1.52SerTyr: 1.52 ± 0.522
0.0SerXaa: 0.0 ± 0.0
Thr
6.238ThrAla: 6.238 ± 0.743
0.48ThrCys: 0.48 ± 0.222
3.759ThrAsp: 3.759 ± 0.622
3.279ThrGlu: 3.279 ± 0.459
2.079ThrPhe: 2.079 ± 0.497
6.398ThrGly: 6.398 ± 1.044
1.679ThrHis: 1.679 ± 0.379
2.719ThrIle: 2.719 ± 0.519
2.879ThrLys: 2.879 ± 0.441
6.318ThrLeu: 6.318 ± 0.859
0.96ThrMet: 0.96 ± 0.28
1.999ThrAsn: 1.999 ± 0.321
2.479ThrPro: 2.479 ± 0.386
4.399ThrGln: 4.399 ± 0.814
2.719ThrArg: 2.719 ± 0.544
3.039ThrSer: 3.039 ± 0.578
3.439ThrThr: 3.439 ± 0.943
4.718ThrVal: 4.718 ± 0.642
0.96ThrTrp: 0.96 ± 0.359
2.719ThrTyr: 2.719 ± 0.849
0.0ThrXaa: 0.0 ± 0.0
Val
4.159ValAla: 4.159 ± 0.665
0.48ValCys: 0.48 ± 0.295
3.439ValAsp: 3.439 ± 0.784
3.999ValGlu: 3.999 ± 0.648
1.599ValPhe: 1.599 ± 0.343
2.799ValGly: 2.799 ± 0.684
1.36ValHis: 1.36 ± 0.277
3.039ValIle: 3.039 ± 0.532
4.639ValLys: 4.639 ± 0.691
3.839ValLeu: 3.839 ± 0.481
1.44ValMet: 1.44 ± 0.334
3.119ValAsn: 3.119 ± 0.471
2.079ValPro: 2.079 ± 0.467
1.839ValGln: 1.839 ± 0.336
3.039ValArg: 3.039 ± 0.8
4.559ValSer: 4.559 ± 0.589
4.319ValThr: 4.319 ± 0.619
3.119ValVal: 3.119 ± 0.687
1.04ValTrp: 1.04 ± 0.324
2.239ValTyr: 2.239 ± 0.489
0.0ValXaa: 0.0 ± 0.0
Trp
1.36TrpAla: 1.36 ± 0.264
0.24TrpCys: 0.24 ± 0.138
1.04TrpAsp: 1.04 ± 0.323
0.48TrpGlu: 0.48 ± 0.121
0.72TrpPhe: 0.72 ± 0.311
0.8TrpGly: 0.8 ± 0.227
0.32TrpHis: 0.32 ± 0.143
0.64TrpIle: 0.64 ± 0.306
0.8TrpLys: 0.8 ± 0.272
1.839TrpLeu: 1.839 ± 0.492
0.32TrpMet: 0.32 ± 0.198
0.64TrpAsn: 0.64 ± 0.23
0.64TrpPro: 0.64 ± 0.27
0.56TrpGln: 0.56 ± 0.211
0.88TrpArg: 0.88 ± 0.256
1.36TrpSer: 1.36 ± 0.346
0.64TrpThr: 0.64 ± 0.178
0.96TrpVal: 0.96 ± 0.295
0.16TrpTrp: 0.16 ± 0.112
0.72TrpTyr: 0.72 ± 0.224
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.039TyrAla: 3.039 ± 0.633
0.32TyrCys: 0.32 ± 0.144
3.039TyrAsp: 3.039 ± 0.709
1.52TyrGlu: 1.52 ± 0.262
0.72TyrPhe: 0.72 ± 0.239
2.719TyrGly: 2.719 ± 0.588
0.8TyrHis: 0.8 ± 0.232
1.759TyrIle: 1.759 ± 0.418
2.079TyrLys: 2.079 ± 0.525
1.759TyrLeu: 1.759 ± 0.421
0.4TyrMet: 0.4 ± 0.16
1.759TyrAsn: 1.759 ± 0.423
1.12TyrPro: 1.12 ± 0.317
2.079TyrGln: 2.079 ± 0.435
2.239TyrArg: 2.239 ± 0.514
2.399TyrSer: 2.399 ± 0.624
2.079TyrThr: 2.079 ± 0.477
1.52TyrVal: 1.52 ± 0.305
0.24TyrTrp: 0.24 ± 0.149
1.36TyrTyr: 1.36 ± 0.394
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 38 proteins (12505 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski