Mycobacterium phage Adler

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 125 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5UPL6|V5UPL6_9CAUD Uncharacterized protein OS=Mycobacterium phage Adler OX=1327959 GN=MaF1660_ph0111 PE=4 SV=1
MM1 pKa = 7.39HH2 pKa = 7.71ALALANTITADD13 pKa = 3.11QVYY16 pKa = 10.2NVVSEE21 pKa = 4.3AGYY24 pKa = 10.87GEE26 pKa = 4.14QFAAFDD32 pKa = 4.16PVSGAVYY39 pKa = 8.6TVYY42 pKa = 8.11WTEE45 pKa = 3.97SGDD48 pKa = 4.18LVGEE52 pKa = 4.36TAPTLSDD59 pKa = 2.91AGEE62 pKa = 4.21VVRR65 pKa = 11.84DD66 pKa = 3.95VIDD69 pKa = 3.95LARR72 pKa = 11.84VPLPLMVWFPAGVPVGWDD90 pKa = 3.11AAVEE94 pKa = 4.06ALASRR99 pKa = 11.84QAVDD103 pKa = 3.18YY104 pKa = 11.19SS105 pKa = 3.49

Molecular weight: 11.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5UPY6|V5UPY6_9CAUD Putative lipoprotein LppP OS=Mycobacterium phage Adler OX=1327959 GN=MaF1660_ph0084 PE=4 SV=1
MM1 pKa = 6.93TQCCPPRR8 pKa = 11.84HH9 pKa = 6.61DD10 pKa = 3.59RR11 pKa = 11.84TPTPSHH17 pKa = 5.08EE18 pKa = 4.39TARR21 pKa = 11.84MFPGDD26 pKa = 3.71CMQYY30 pKa = 10.05PSAAEE35 pKa = 3.77RR36 pKa = 11.84AEE38 pKa = 3.85ASALIGKK45 pKa = 8.97PIMGGNRR52 pKa = 11.84AYY54 pKa = 10.91LRR56 pKa = 11.84AQVGRR61 pKa = 11.84WLVKK65 pKa = 8.38TGRR68 pKa = 11.84WSSRR72 pKa = 11.84NVRR75 pKa = 11.84PP76 pKa = 3.9

Molecular weight: 8.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

125

0

125

28636

41

1405

229.1

24.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.535 ± 0.35

0.772 ± 0.069

6.087 ± 0.16

5.399 ± 0.221

2.584 ± 0.143

8.283 ± 0.371

2.525 ± 0.123

4.285 ± 0.131

2.664 ± 0.165

8.287 ± 0.238

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.396 ± 0.119

2.884 ± 0.11

6.607 ± 0.302

4.009 ± 0.141

7.012 ± 0.217

5.444 ± 0.186

6.066 ± 0.139

7.089 ± 0.195

1.833 ± 0.103

2.238 ± 0.096
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski