Amino acid dipepetide frequency for Mycobacterium phage Adler

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.119AlaAla: 23.119 ± 1.629
0.908AlaCys: 0.908 ± 0.174
8.835AlaAsp: 8.835 ± 0.655
8.172AlaGlu: 8.172 ± 0.481
2.759AlaPhe: 2.759 ± 0.379
12.013AlaGly: 12.013 ± 0.903
3.597AlaHis: 3.597 ± 0.394
5.483AlaIle: 5.483 ± 0.452
3.772AlaLys: 3.772 ± 0.396
11.035AlaLeu: 11.035 ± 0.652
2.968AlaMet: 2.968 ± 0.251
3.527AlaAsn: 3.527 ± 0.367
7.753AlaPro: 7.753 ± 0.737
5.553AlaGln: 5.553 ± 0.439
8.905AlaArg: 8.905 ± 0.64
5.343AlaSer: 5.343 ± 0.428
7.089AlaThr: 7.089 ± 0.554
10.127AlaVal: 10.127 ± 0.69
2.095AlaTrp: 2.095 ± 0.257
2.305AlaTyr: 2.305 ± 0.3
0.0AlaXaa: 0.0 ± 0.0
Cys
0.838CysAla: 0.838 ± 0.191
0.175CysCys: 0.175 ± 0.094
0.454CysAsp: 0.454 ± 0.125
0.21CysGlu: 0.21 ± 0.086
0.349CysPhe: 0.349 ± 0.126
0.943CysGly: 0.943 ± 0.218
0.21CysHis: 0.21 ± 0.085
0.384CysIle: 0.384 ± 0.133
0.14CysLys: 0.14 ± 0.087
0.698CysLeu: 0.698 ± 0.19
0.314CysMet: 0.314 ± 0.098
0.279CysAsn: 0.279 ± 0.11
0.594CysPro: 0.594 ± 0.156
0.279CysGln: 0.279 ± 0.099
0.524CysArg: 0.524 ± 0.144
0.21CysSer: 0.21 ± 0.089
0.489CysThr: 0.489 ± 0.17
0.314CysVal: 0.314 ± 0.106
0.14CysTrp: 0.14 ± 0.073
0.175CysTyr: 0.175 ± 0.072
0.0CysXaa: 0.0 ± 0.0
Asp
6.635AspAla: 6.635 ± 0.488
0.629AspCys: 0.629 ± 0.129
2.968AspAsp: 2.968 ± 0.362
3.946AspGlu: 3.946 ± 0.379
1.467AspPhe: 1.467 ± 0.241
4.365AspGly: 4.365 ± 0.418
1.467AspHis: 1.467 ± 0.193
2.864AspIle: 2.864 ± 0.308
1.851AspLys: 1.851 ± 0.276
5.727AspLeu: 5.727 ± 0.47
1.257AspMet: 1.257 ± 0.236
1.851AspAsn: 1.851 ± 0.277
5.622AspPro: 5.622 ± 0.506
2.759AspGln: 2.759 ± 0.303
4.121AspArg: 4.121 ± 0.383
3.283AspSer: 3.283 ± 0.269
3.213AspThr: 3.213 ± 0.27
4.261AspVal: 4.261 ± 0.451
1.152AspTrp: 1.152 ± 0.17
1.432AspTyr: 1.432 ± 0.229
0.0AspXaa: 0.0 ± 0.0
Glu
7.159GluAla: 7.159 ± 0.621
0.314GluCys: 0.314 ± 0.084
2.654GluAsp: 2.654 ± 0.288
2.025GluGlu: 2.025 ± 0.289
1.362GluPhe: 1.362 ± 0.199
3.737GluGly: 3.737 ± 0.32
1.711GluHis: 1.711 ± 0.254
1.851GluIle: 1.851 ± 0.245
1.676GluLys: 1.676 ± 0.176
5.553GluLeu: 5.553 ± 0.435
1.222GluMet: 1.222 ± 0.195
2.06GluAsn: 2.06 ± 0.31
3.492GluPro: 3.492 ± 0.401
2.829GluGln: 2.829 ± 0.265
4.645GluArg: 4.645 ± 0.45
2.654GluSer: 2.654 ± 0.354
3.283GluThr: 3.283 ± 0.334
3.108GluVal: 3.108 ± 0.443
1.118GluTrp: 1.118 ± 0.19
1.537GluTyr: 1.537 ± 0.225
0.0GluXaa: 0.0 ± 0.0
Phe
3.248PheAla: 3.248 ± 0.362
0.21PheCys: 0.21 ± 0.09
1.781PheAsp: 1.781 ± 0.253
1.362PheGlu: 1.362 ± 0.296
0.489PhePhe: 0.489 ± 0.171
2.759PheGly: 2.759 ± 0.375
0.489PheHis: 0.489 ± 0.126
1.222PheIle: 1.222 ± 0.24
1.048PheLys: 1.048 ± 0.199
1.886PheLeu: 1.886 ± 0.332
0.733PheMet: 0.733 ± 0.179
0.768PheAsn: 0.768 ± 0.147
1.467PhePro: 1.467 ± 0.238
0.978PheGln: 0.978 ± 0.203
1.222PheArg: 1.222 ± 0.21
1.327PheSer: 1.327 ± 0.238
1.851PheThr: 1.851 ± 0.252
1.502PheVal: 1.502 ± 0.205
0.524PheTrp: 0.524 ± 0.165
0.978PheTyr: 0.978 ± 0.175
0.0PheXaa: 0.0 ± 0.0
Gly
9.883GlyAla: 9.883 ± 0.737
0.664GlyCys: 0.664 ± 0.168
4.47GlyAsp: 4.47 ± 0.366
4.191GlyGlu: 4.191 ± 0.422
2.025GlyPhe: 2.025 ± 0.295
8.242GlyGly: 8.242 ± 1.036
2.165GlyHis: 2.165 ± 0.297
3.003GlyIle: 3.003 ± 0.333
2.375GlyLys: 2.375 ± 0.34
7.648GlyLeu: 7.648 ± 0.608
2.235GlyMet: 2.235 ± 0.315
2.06GlyAsn: 2.06 ± 0.268
5.448GlyPro: 5.448 ± 0.536
3.807GlyGln: 3.807 ± 0.504
5.832GlyArg: 5.832 ± 0.504
5.169GlySer: 5.169 ± 0.613
4.505GlyThr: 4.505 ± 0.447
5.343GlyVal: 5.343 ± 0.496
1.467GlyTrp: 1.467 ± 0.255
2.305GlyTyr: 2.305 ± 0.252
0.0GlyXaa: 0.0 ± 0.0
His
3.213HisAla: 3.213 ± 0.28
0.244HisCys: 0.244 ± 0.095
1.606HisAsp: 1.606 ± 0.22
1.118HisGlu: 1.118 ± 0.177
0.559HisPhe: 0.559 ± 0.142
1.921HisGly: 1.921 ± 0.227
0.664HisHis: 0.664 ± 0.144
1.013HisIle: 1.013 ± 0.204
0.698HisLys: 0.698 ± 0.16
2.27HisLeu: 2.27 ± 0.309
0.768HisMet: 0.768 ± 0.168
0.803HisAsn: 0.803 ± 0.158
1.397HisPro: 1.397 ± 0.201
1.048HisGln: 1.048 ± 0.189
2.27HisArg: 2.27 ± 0.348
0.943HisSer: 0.943 ± 0.154
1.572HisThr: 1.572 ± 0.24
1.886HisVal: 1.886 ± 0.301
0.698HisTrp: 0.698 ± 0.166
0.524HisTyr: 0.524 ± 0.149
0.0HisXaa: 0.0 ± 0.0
Ile
7.438IleAla: 7.438 ± 0.535
0.21IleCys: 0.21 ± 0.078
3.457IleAsp: 3.457 ± 0.341
3.003IleGlu: 3.003 ± 0.331
0.664IlePhe: 0.664 ± 0.134
3.702IleGly: 3.702 ± 0.379
0.698IleHis: 0.698 ± 0.137
2.095IleIle: 2.095 ± 0.311
0.908IleLys: 0.908 ± 0.184
2.654IleLeu: 2.654 ± 0.326
0.768IleMet: 0.768 ± 0.142
1.187IleAsn: 1.187 ± 0.216
2.375IlePro: 2.375 ± 0.28
1.257IleGln: 1.257 ± 0.237
2.829IleArg: 2.829 ± 0.303
1.432IleSer: 1.432 ± 0.19
3.178IleThr: 3.178 ± 0.332
3.248IleVal: 3.248 ± 0.394
0.733IleTrp: 0.733 ± 0.154
1.013IleTyr: 1.013 ± 0.183
0.0IleXaa: 0.0 ± 0.0
Lys
3.667LysAla: 3.667 ± 0.365
0.14LysCys: 0.14 ± 0.072
1.781LysAsp: 1.781 ± 0.249
1.572LysGlu: 1.572 ± 0.187
0.803LysPhe: 0.803 ± 0.193
1.606LysGly: 1.606 ± 0.265
0.629LysHis: 0.629 ± 0.176
0.873LysIle: 0.873 ± 0.201
1.013LysLys: 1.013 ± 0.21
3.003LysLeu: 3.003 ± 0.355
0.664LysMet: 0.664 ± 0.136
0.629LysAsn: 0.629 ± 0.185
1.711LysPro: 1.711 ± 0.293
0.768LysGln: 0.768 ± 0.165
1.572LysArg: 1.572 ± 0.282
1.432LysSer: 1.432 ± 0.283
1.851LysThr: 1.851 ± 0.224
1.921LysVal: 1.921 ± 0.262
0.454LysTrp: 0.454 ± 0.119
0.559LysTyr: 0.559 ± 0.129
0.0LysXaa: 0.0 ± 0.0
Leu
11.559LeuAla: 11.559 ± 0.716
0.629LeuCys: 0.629 ± 0.165
5.762LeuAsp: 5.762 ± 0.438
3.527LeuGlu: 3.527 ± 0.405
2.549LeuPhe: 2.549 ± 0.322
7.159LeuGly: 7.159 ± 0.567
2.235LeuHis: 2.235 ± 0.271
4.226LeuIle: 4.226 ± 0.437
1.886LeuLys: 1.886 ± 0.31
6.705LeuLeu: 6.705 ± 0.567
1.921LeuMet: 1.921 ± 0.288
2.165LeuAsn: 2.165 ± 0.296
5.099LeuPro: 5.099 ± 0.336
2.479LeuGln: 2.479 ± 0.328
6.042LeuArg: 6.042 ± 0.472
5.169LeuSer: 5.169 ± 0.415
5.029LeuThr: 5.029 ± 0.507
5.622LeuVal: 5.622 ± 0.449
1.292LeuTrp: 1.292 ± 0.188
1.816LeuTyr: 1.816 ± 0.26
0.0LeuXaa: 0.0 ± 0.0
Met
2.41MetAla: 2.41 ± 0.306
0.14MetCys: 0.14 ± 0.067
1.362MetAsp: 1.362 ± 0.19
0.594MetGlu: 0.594 ± 0.142
1.048MetPhe: 1.048 ± 0.213
1.921MetGly: 1.921 ± 0.27
0.454MetHis: 0.454 ± 0.128
0.943MetIle: 0.943 ± 0.209
0.698MetLys: 0.698 ± 0.157
2.165MetLeu: 2.165 ± 0.296
0.559MetMet: 0.559 ± 0.16
0.838MetAsn: 0.838 ± 0.179
1.606MetPro: 1.606 ± 0.268
0.803MetGln: 0.803 ± 0.175
1.711MetArg: 1.711 ± 0.269
1.991MetSer: 1.991 ± 0.201
1.921MetThr: 1.921 ± 0.284
1.711MetVal: 1.711 ± 0.242
0.629MetTrp: 0.629 ± 0.14
0.454MetTyr: 0.454 ± 0.114
0.0MetXaa: 0.0 ± 0.0
Asn
3.353AsnAla: 3.353 ± 0.4
0.175AsnCys: 0.175 ± 0.076
1.921AsnAsp: 1.921 ± 0.254
1.432AsnGlu: 1.432 ± 0.229
0.489AsnPhe: 0.489 ± 0.101
2.584AsnGly: 2.584 ± 0.283
0.803AsnHis: 0.803 ± 0.188
1.048AsnIle: 1.048 ± 0.182
1.048AsnLys: 1.048 ± 0.177
2.13AsnLeu: 2.13 ± 0.248
0.664AsnMet: 0.664 ± 0.141
1.397AsnAsn: 1.397 ± 0.25
2.933AsnPro: 2.933 ± 0.361
1.013AsnGln: 1.013 ± 0.182
1.746AsnArg: 1.746 ± 0.261
1.676AsnSer: 1.676 ± 0.262
1.641AsnThr: 1.641 ± 0.226
1.816AsnVal: 1.816 ± 0.232
0.384AsnTrp: 0.384 ± 0.092
0.594AsnTyr: 0.594 ± 0.153
0.0AsnXaa: 0.0 ± 0.0
Pro
8.556ProAla: 8.556 ± 0.73
0.21ProCys: 0.21 ± 0.093
4.47ProAsp: 4.47 ± 0.444
4.715ProGlu: 4.715 ± 0.461
1.292ProPhe: 1.292 ± 0.229
5.902ProGly: 5.902 ± 0.525
1.467ProHis: 1.467 ± 0.219
2.654ProIle: 2.654 ± 0.304
1.781ProLys: 1.781 ± 0.283
4.261ProLeu: 4.261 ± 0.432
1.397ProMet: 1.397 ± 0.231
1.921ProAsn: 1.921 ± 0.413
5.657ProPro: 5.657 ± 0.869
2.549ProGln: 2.549 ± 0.279
3.876ProArg: 3.876 ± 0.453
4.086ProSer: 4.086 ± 0.41
3.911ProThr: 3.911 ± 0.392
5.134ProVal: 5.134 ± 0.426
1.152ProTrp: 1.152 ± 0.219
1.083ProTyr: 1.083 ± 0.187
0.0ProXaa: 0.0 ± 0.0
Gln
4.924GlnAla: 4.924 ± 0.49
0.454GlnCys: 0.454 ± 0.12
1.746GlnAsp: 1.746 ± 0.255
2.165GlnGlu: 2.165 ± 0.286
1.432GlnPhe: 1.432 ± 0.306
2.549GlnGly: 2.549 ± 0.302
1.083GlnHis: 1.083 ± 0.194
2.445GlnIle: 2.445 ± 0.277
0.873GlnLys: 0.873 ± 0.239
3.841GlnLeu: 3.841 ± 0.422
1.222GlnMet: 1.222 ± 0.231
0.978GlnAsn: 0.978 ± 0.168
1.991GlnPro: 1.991 ± 0.299
2.2GlnGln: 2.2 ± 0.296
2.829GlnArg: 2.829 ± 0.341
2.2GlnSer: 2.2 ± 0.323
1.956GlnThr: 1.956 ± 0.297
3.213GlnVal: 3.213 ± 0.369
0.943GlnTrp: 0.943 ± 0.187
1.048GlnTyr: 1.048 ± 0.197
0.0GlnXaa: 0.0 ± 0.0
Arg
9.254ArgAla: 9.254 ± 0.535
0.698ArgCys: 0.698 ± 0.182
3.807ArgAsp: 3.807 ± 0.469
3.981ArgGlu: 3.981 ± 0.365
1.956ArgPhe: 1.956 ± 0.257
4.68ArgGly: 4.68 ± 0.403
2.305ArgHis: 2.305 ± 0.313
2.829ArgIle: 2.829 ± 0.304
1.676ArgLys: 1.676 ± 0.217
6.007ArgLeu: 6.007 ± 0.488
1.781ArgMet: 1.781 ± 0.248
2.06ArgAsn: 2.06 ± 0.231
4.54ArgPro: 4.54 ± 0.438
3.038ArgGln: 3.038 ± 0.341
5.902ArgArg: 5.902 ± 0.826
3.492ArgSer: 3.492 ± 0.394
4.715ArgThr: 4.715 ± 0.452
4.47ArgVal: 4.47 ± 0.355
1.327ArgTrp: 1.327 ± 0.204
1.606ArgTyr: 1.606 ± 0.238
0.0ArgXaa: 0.0 ± 0.0
Ser
8.067SerAla: 8.067 ± 0.556
0.175SerCys: 0.175 ± 0.078
3.108SerAsp: 3.108 ± 0.334
3.213SerGlu: 3.213 ± 0.348
1.467SerPhe: 1.467 ± 0.213
5.169SerGly: 5.169 ± 0.608
0.873SerHis: 0.873 ± 0.156
2.2SerIle: 2.2 ± 0.22
1.083SerLys: 1.083 ± 0.215
3.283SerLeu: 3.283 ± 0.302
1.676SerMet: 1.676 ± 0.248
1.222SerAsn: 1.222 ± 0.202
3.632SerPro: 3.632 ± 0.352
2.375SerGln: 2.375 ± 0.265
3.702SerArg: 3.702 ± 0.363
3.422SerSer: 3.422 ± 0.491
3.318SerThr: 3.318 ± 0.294
4.226SerVal: 4.226 ± 0.339
1.222SerTrp: 1.222 ± 0.212
1.013SerTyr: 1.013 ± 0.199
0.0SerXaa: 0.0 ± 0.0
Thr
8.172ThrAla: 8.172 ± 0.561
0.489ThrCys: 0.489 ± 0.156
4.191ThrAsp: 4.191 ± 0.373
3.143ThrGlu: 3.143 ± 0.35
1.711ThrPhe: 1.711 ± 0.245
5.029ThrGly: 5.029 ± 0.393
1.432ThrHis: 1.432 ± 0.2
2.41ThrIle: 2.41 ± 0.253
1.327ThrLys: 1.327 ± 0.239
4.47ThrLeu: 4.47 ± 0.39
1.257ThrMet: 1.257 ± 0.237
1.606ThrAsn: 1.606 ± 0.248
4.4ThrPro: 4.4 ± 0.472
2.06ThrGln: 2.06 ± 0.272
4.4ThrArg: 4.4 ± 0.348
3.772ThrSer: 3.772 ± 0.413
3.911ThrThr: 3.911 ± 0.353
4.784ThrVal: 4.784 ± 0.414
0.768ThrTrp: 0.768 ± 0.161
1.327ThrTyr: 1.327 ± 0.243
0.0ThrXaa: 0.0 ± 0.0
Val
8.87ValAla: 8.87 ± 0.63
0.629ValCys: 0.629 ± 0.189
4.261ValAsp: 4.261 ± 0.415
3.213ValGlu: 3.213 ± 0.336
2.165ValPhe: 2.165 ± 0.285
6.007ValGly: 6.007 ± 0.599
1.991ValHis: 1.991 ± 0.322
3.772ValIle: 3.772 ± 0.395
1.921ValLys: 1.921 ± 0.228
5.972ValLeu: 5.972 ± 0.537
1.606ValMet: 1.606 ± 0.198
2.27ValAsn: 2.27 ± 0.238
3.946ValPro: 3.946 ± 0.391
2.619ValGln: 2.619 ± 0.302
4.819ValArg: 4.819 ± 0.39
4.54ValSer: 4.54 ± 0.415
4.819ValThr: 4.819 ± 0.412
4.505ValVal: 4.505 ± 0.458
1.222ValTrp: 1.222 ± 0.246
1.746ValTyr: 1.746 ± 0.285
0.0ValXaa: 0.0 ± 0.0
Trp
2.095TrpAla: 2.095 ± 0.268
0.279TrpCys: 0.279 ± 0.095
1.083TrpAsp: 1.083 ± 0.203
0.873TrpGlu: 0.873 ± 0.165
0.698TrpPhe: 0.698 ± 0.145
0.768TrpGly: 0.768 ± 0.189
0.244TrpHis: 0.244 ± 0.084
0.838TrpIle: 0.838 ± 0.174
0.489TrpLys: 0.489 ± 0.152
1.606TrpLeu: 1.606 ± 0.245
0.419TrpMet: 0.419 ± 0.126
0.559TrpAsn: 0.559 ± 0.135
1.292TrpPro: 1.292 ± 0.2
0.803TrpGln: 0.803 ± 0.16
1.467TrpArg: 1.467 ± 0.194
1.432TrpSer: 1.432 ± 0.187
0.733TrpThr: 0.733 ± 0.165
1.676TrpVal: 1.676 ± 0.292
0.524TrpTrp: 0.524 ± 0.11
0.454TrpTyr: 0.454 ± 0.136
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.899TyrAla: 2.899 ± 0.218
0.349TyrCys: 0.349 ± 0.123
1.152TyrAsp: 1.152 ± 0.217
1.537TyrGlu: 1.537 ± 0.256
0.559TyrPhe: 0.559 ± 0.133
1.781TyrGly: 1.781 ± 0.227
0.733TyrHis: 0.733 ± 0.146
0.698TyrIle: 0.698 ± 0.162
0.384TyrLys: 0.384 ± 0.102
1.956TyrLeu: 1.956 ± 0.288
0.489TyrMet: 0.489 ± 0.137
0.664TyrAsn: 0.664 ± 0.157
1.118TyrPro: 1.118 ± 0.187
0.873TyrGln: 0.873 ± 0.169
1.711TyrArg: 1.711 ± 0.226
0.873TyrSer: 0.873 ± 0.17
1.676TyrThr: 1.676 ± 0.232
2.025TyrVal: 2.025 ± 0.293
0.489TyrTrp: 0.489 ± 0.134
0.419TyrTyr: 0.419 ± 0.124
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 125 proteins (28636 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski