Mycobacterium phage Vanisoa

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TE06|A0A5J6TE06_9CAUD Uncharacterized protein OS=Mycobacterium phage Vanisoa OX=2599883 GN=57 PE=4 SV=1
MM1 pKa = 7.58PRR3 pKa = 11.84ATYY6 pKa = 10.97VNFVDD11 pKa = 4.71RR12 pKa = 11.84VQFFAGEE19 pKa = 4.09PMLDD23 pKa = 3.25TSEE26 pKa = 4.14GVLVIQYY33 pKa = 9.85PDD35 pKa = 3.36GSSRR39 pKa = 11.84TLNWDD44 pKa = 3.55FVIDD48 pKa = 4.04FYY50 pKa = 12.12YY51 pKa = 10.26MSEE54 pKa = 4.34EE55 pKa = 4.01EE56 pKa = 4.08TADD59 pKa = 4.8AIQQSAQEE67 pKa = 4.18EE68 pKa = 4.41DD69 pKa = 4.06DD70 pKa = 4.34NEE72 pKa = 4.08

Molecular weight: 8.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TCL1|A0A5J6TCL1_9CAUD Uncharacterized protein OS=Mycobacterium phage Vanisoa OX=2599883 GN=71 PE=4 SV=1
MM1 pKa = 7.35SWADD5 pKa = 2.94SDD7 pKa = 4.61RR8 pKa = 11.84RR9 pKa = 11.84QNLPADD15 pKa = 3.66WEE17 pKa = 4.18QRR19 pKa = 11.84RR20 pKa = 11.84LSVLVDD26 pKa = 3.53AEE28 pKa = 4.27WLCEE32 pKa = 3.89IEE34 pKa = 4.21MAGCTRR40 pKa = 11.84VATDD44 pKa = 2.9VDD46 pKa = 3.91HH47 pKa = 7.0VKK49 pKa = 10.57RR50 pKa = 11.84GNDD53 pKa = 2.94HH54 pKa = 5.92SRR56 pKa = 11.84RR57 pKa = 11.84NLRR60 pKa = 11.84AACGWCHH67 pKa = 6.24DD68 pKa = 4.39RR69 pKa = 11.84KK70 pKa = 10.78SSAEE74 pKa = 3.67GVARR78 pKa = 11.84RR79 pKa = 11.84RR80 pKa = 11.84EE81 pKa = 3.88LKK83 pKa = 10.28ARR85 pKa = 11.84RR86 pKa = 11.84KK87 pKa = 9.71RR88 pKa = 11.84PPEE91 pKa = 3.6RR92 pKa = 11.84HH93 pKa = 5.7PGRR96 pKa = 11.84RR97 pKa = 3.38

Molecular weight: 11.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

16701

40

972

181.5

19.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.353 ± 0.377

0.778 ± 0.116

6.299 ± 0.18

6.419 ± 0.281

3.383 ± 0.168

8.461 ± 0.451

1.838 ± 0.164

5.072 ± 0.165

4.413 ± 0.218

8.149 ± 0.221

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.563 ± 0.171

3.605 ± 0.17

5.239 ± 0.225

3.491 ± 0.185

6.227 ± 0.311

5.646 ± 0.233

6.299 ± 0.234

7.024 ± 0.189

1.952 ± 0.127

2.79 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski