Amino acid dipepetide frequency for Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.198AlaAla: 10.198 ± 0.081	0.92AlaCys: 0.92 ± 0.015	4.182AlaAsp: 4.182 ± 0.031	5.428AlaGlu: 5.428 ± 0.054	2.767AlaPhe: 2.767 ± 0.024	6.004AlaGly: 6.004 ± 0.037	1.78AlaHis: 1.78 ± 0.019	3.852AlaIle: 3.852 ± 0.034	4.346AlaLys: 4.346 ± 0.038	7.149AlaLeu: 7.149 ± 0.047	1.944AlaMet: 1.944 ± 0.019	2.961AlaAsn: 2.961 ± 0.028	5.404AlaPro: 5.404 ± 0.048	3.551AlaGln: 3.551 ± 0.037	4.962AlaArg: 4.962 ± 0.038	7.451AlaSer: 7.451 ± 0.048	5.546AlaThr: 5.546 ± 0.035	5.343AlaVal: 5.343 ± 0.038	1.021AlaTrp: 1.021 ± 0.013	1.957AlaTyr: 1.957 ± 0.021	0.0AlaXaa: 0.0 ± 0.0
Cys	0.83CysAla: 0.83 ± 0.016	0.237CysCys: 0.237 ± 0.007	0.577CysAsp: 0.577 ± 0.011	0.548CysGlu: 0.548 ± 0.011	0.47CysPhe: 0.47 ± 0.01	0.865CysGly: 0.865 ± 0.018	0.313CysHis: 0.313 ± 0.008	0.584CysIle: 0.584 ± 0.012	0.5CysLys: 0.5 ± 0.012	1.115CysLeu: 1.115 ± 0.018	0.239CysMet: 0.239 ± 0.007	0.419CysAsn: 0.419 ± 0.01	0.674CysPro: 0.674 ± 0.012	0.413CysGln: 0.413 ± 0.009	0.693CysArg: 0.693 ± 0.013	0.817CysSer: 0.817 ± 0.014	0.622CysThr: 0.622 ± 0.012	0.703CysVal: 0.703 ± 0.013	0.19CysTrp: 0.19 ± 0.008	0.311CysTyr: 0.311 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	4.5AspAla: 4.5 ± 0.034	0.571AspCys: 0.571 ± 0.011	4.743AspAsp: 4.743 ± 0.053	4.781AspGlu: 4.781 ± 0.048	2.053AspPhe: 2.053 ± 0.022	4.117AspGly: 4.117 ± 0.031	1.32AspHis: 1.32 ± 0.016	2.763AspIle: 2.763 ± 0.023	2.575AspLys: 2.575 ± 0.025	4.765AspLeu: 4.765 ± 0.038	1.297AspMet: 1.297 ± 0.017	2.005AspAsn: 2.005 ± 0.02	3.332AspPro: 3.332 ± 0.03	1.905AspGln: 1.905 ± 0.022	3.029AspArg: 3.029 ± 0.035	3.991AspSer: 3.991 ± 0.036	2.8AspThr: 2.8 ± 0.022	3.494AspVal: 3.494 ± 0.028	0.835AspTrp: 0.835 ± 0.013	1.546AspTyr: 1.546 ± 0.02	0.0AspXaa: 0.0 ± 0.0
Glu	5.65GluAla: 5.65 ± 0.051	0.577GluCys: 0.577 ± 0.011	4.496GluAsp: 4.496 ± 0.044	7.01GluGlu: 7.01 ± 0.086	1.85GluPhe: 1.85 ± 0.02	4.133GluGly: 4.133 ± 0.035	1.414GluHis: 1.414 ± 0.019	2.782GluIle: 2.782 ± 0.028	4.144GluLys: 4.144 ± 0.049	5.209GluLeu: 5.209 ± 0.056	1.52GluMet: 1.52 ± 0.019	2.238GluAsn: 2.238 ± 0.019	3.004GluPro: 3.004 ± 0.043	2.779GluGln: 2.779 ± 0.036	4.262GluArg: 4.262 ± 0.047	4.076GluSer: 4.076 ± 0.035	3.439GluThr: 3.439 ± 0.03	3.827GluVal: 3.827 ± 0.042	0.878GluTrp: 0.878 ± 0.014	1.572GluTyr: 1.572 ± 0.019	0.0GluXaa: 0.0 ± 0.0
Phe	2.786PheAla: 2.786 ± 0.024	0.497PheCys: 0.497 ± 0.01	2.107PheAsp: 2.107 ± 0.023	1.992PheGlu: 1.992 ± 0.021	1.442PhePhe: 1.442 ± 0.02	2.689PheGly: 2.689 ± 0.033	0.852PheHis: 0.852 ± 0.015	1.532PheIle: 1.532 ± 0.02	1.448PheLys: 1.448 ± 0.018	3.079PheLeu: 3.079 ± 0.027	0.727PheMet: 0.727 ± 0.011	1.337PheAsn: 1.337 ± 0.016	1.881PhePro: 1.881 ± 0.021	1.232PheGln: 1.232 ± 0.015	1.841PheArg: 1.841 ± 0.019	2.77PheSer: 2.77 ± 0.024	2.008PheThr: 2.008 ± 0.021	2.153PheVal: 2.153 ± 0.024	0.562PheTrp: 0.562 ± 0.012	0.958PheTyr: 0.958 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	5.415GlyAla: 5.415 ± 0.041	0.816GlyCys: 0.816 ± 0.015	3.686GlyAsp: 3.686 ± 0.032	3.945GlyGlu: 3.945 ± 0.032	2.612GlyPhe: 2.612 ± 0.028	7.495GlyGly: 7.495 ± 0.087	1.846GlyHis: 1.846 ± 0.02	3.16GlyIle: 3.16 ± 0.033	3.623GlyLys: 3.623 ± 0.03	5.758GlyLeu: 5.758 ± 0.04	1.672GlyMet: 1.672 ± 0.019	2.786GlyAsn: 2.786 ± 0.027	3.678GlyPro: 3.678 ± 0.035	2.82GlyGln: 2.82 ± 0.03	4.353GlyArg: 4.353 ± 0.035	6.117GlySer: 6.117 ± 0.05	4.144GlyThr: 4.144 ± 0.033	4.306GlyVal: 4.306 ± 0.038	1.12GlyTrp: 1.12 ± 0.015	2.088GlyTyr: 2.088 ± 0.023	0.0GlyXaa: 0.0 ± 0.0
His	1.797HisAla: 1.797 ± 0.019	0.312HisCys: 0.312 ± 0.008	1.355HisAsp: 1.355 ± 0.017	1.345HisGlu: 1.345 ± 0.017	0.859HisPhe: 0.859 ± 0.015	1.846HisGly: 1.846 ± 0.019	1.226HisHis: 1.226 ± 0.021	1.114HisIle: 1.114 ± 0.016	0.961HisLys: 0.961 ± 0.014	2.15HisLeu: 2.15 ± 0.02	0.488HisMet: 0.488 ± 0.009	0.93HisAsn: 0.93 ± 0.015	1.772HisPro: 1.772 ± 0.021	1.277HisGln: 1.277 ± 0.02	1.583HisArg: 1.583 ± 0.019	1.893HisSer: 1.893 ± 0.026	1.269HisThr: 1.269 ± 0.015	1.386HisVal: 1.386 ± 0.018	0.306HisTrp: 0.306 ± 0.007	0.695HisTyr: 0.695 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	3.697IleAla: 3.697 ± 0.032	0.617IleCys: 0.617 ± 0.012	2.595IleAsp: 2.595 ± 0.024	2.648IleGlu: 2.648 ± 0.026	1.678IlePhe: 1.678 ± 0.02	2.751IleGly: 2.751 ± 0.029	1.098IleHis: 1.098 ± 0.015	2.2IleIle: 2.2 ± 0.022	2.213IleLys: 2.213 ± 0.024	3.93IleLeu: 3.93 ± 0.033	0.955IleMet: 0.955 ± 0.015	1.7IleAsn: 1.7 ± 0.02	3.033IlePro: 3.033 ± 0.025	1.754IleGln: 1.754 ± 0.02	2.622IleArg: 2.622 ± 0.026	3.425IleSer: 3.425 ± 0.025	2.764IleThr: 2.764 ± 0.024	2.792IleVal: 2.792 ± 0.027	0.633IleTrp: 0.633 ± 0.013	1.193IleTyr: 1.193 ± 0.018	0.0IleXaa: 0.0 ± 0.0
Lys	4.523LysAla: 4.523 ± 0.042	0.484LysCys: 0.484 ± 0.009	2.857LysAsp: 2.857 ± 0.032	4.01LysGlu: 4.01 ± 0.043	1.426LysPhe: 1.426 ± 0.015	3.265LysGly: 3.265 ± 0.03	1.136LysHis: 1.136 ± 0.015	2.164LysIle: 2.164 ± 0.023	4.062LysLys: 4.062 ± 0.047	4.029LysLeu: 4.029 ± 0.034	1.078LysMet: 1.078 ± 0.016	1.781LysAsn: 1.781 ± 0.022	2.992LysPro: 2.992 ± 0.031	2.01LysGln: 2.01 ± 0.021	3.643LysArg: 3.643 ± 0.029	3.444LysSer: 3.444 ± 0.032	2.975LysThr: 2.975 ± 0.026	2.914LysVal: 2.914 ± 0.031	0.692LysTrp: 0.692 ± 0.014	1.278LysTyr: 1.278 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	7.391LeuAla: 7.391 ± 0.047	1.067LeuCys: 1.067 ± 0.017	4.852LeuAsp: 4.852 ± 0.038	5.304LeuGlu: 5.304 ± 0.047	3.006LeuPhe: 3.006 ± 0.029	5.6LeuGly: 5.6 ± 0.038	2.04LeuHis: 2.04 ± 0.023	3.435LeuIle: 3.435 ± 0.031	4.145LeuLys: 4.145 ± 0.037	7.648LeuLeu: 7.648 ± 0.056	1.688LeuMet: 1.688 ± 0.02	2.984LeuAsn: 2.984 ± 0.026	5.681LeuPro: 5.681 ± 0.069	3.503LeuGln: 3.503 ± 0.035	5.434LeuArg: 5.434 ± 0.038	6.819LeuSer: 6.819 ± 0.049	4.623LeuThr: 4.623 ± 0.036	5.052LeuVal: 5.052 ± 0.036	1.092LeuTrp: 1.092 ± 0.015	2.094LeuTyr: 2.094 ± 0.021	0.0LeuXaa: 0.0 ± 0.0
Met	2.251MetAla: 2.251 ± 0.025	0.225MetCys: 0.225 ± 0.006	1.286MetAsp: 1.286 ± 0.018	1.372MetGlu: 1.372 ± 0.02	0.685MetPhe: 0.685 ± 0.012	1.598MetGly: 1.598 ± 0.019	0.441MetHis: 0.441 ± 0.009	0.881MetIle: 0.881 ± 0.012	1.002MetLys: 1.002 ± 0.015	1.723MetLeu: 1.723 ± 0.021	0.618MetMet: 0.618 ± 0.011	0.756MetAsn: 0.756 ± 0.012	1.312MetPro: 1.312 ± 0.018	0.81MetGln: 0.81 ± 0.014	1.248MetArg: 1.248 ± 0.015	1.89MetSer: 1.89 ± 0.02	1.333MetThr: 1.333 ± 0.016	1.343MetVal: 1.343 ± 0.017	0.25MetTrp: 0.25 ± 0.007	0.487MetTyr: 0.487 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	2.958AsnAla: 2.958 ± 0.026	0.397AsnCys: 0.397 ± 0.011	1.992AsnAsp: 1.992 ± 0.022	1.96AsnGlu: 1.96 ± 0.02	1.274AsnPhe: 1.274 ± 0.016	3.218AsnGly: 3.218 ± 0.031	0.947AsnHis: 0.947 ± 0.016	1.823AsnIle: 1.823 ± 0.02	1.775AsnLys: 1.775 ± 0.02	3.071AsnLeu: 3.071 ± 0.028	0.875AsnMet: 0.875 ± 0.014	2.046AsnAsn: 2.046 ± 0.043	2.611AsnPro: 2.611 ± 0.025	1.413AsnGln: 1.413 ± 0.02	1.983AsnArg: 1.983 ± 0.023	2.817AsnSer: 2.817 ± 0.029	2.253AsnThr: 2.253 ± 0.021	2.156AsnVal: 2.156 ± 0.02	0.518AsnTrp: 0.518 ± 0.011	1.024AsnTyr: 1.024 ± 0.017	0.0AsnXaa: 0.0 ± 0.0
Pro	5.926ProAla: 5.926 ± 0.069	0.511ProCys: 0.511 ± 0.012	3.176ProAsp: 3.176 ± 0.027	4.004ProGlu: 4.004 ± 0.063	1.977ProPhe: 1.977 ± 0.021	4.297ProGly: 4.297 ± 0.039	1.438ProHis: 1.438 ± 0.018	2.582ProIle: 2.582 ± 0.024	2.964ProLys: 2.964 ± 0.03	4.85ProLeu: 4.85 ± 0.036	1.145ProMet: 1.145 ± 0.017	2.344ProAsn: 2.344 ± 0.021	6.495ProPro: 6.495 ± 0.084	2.807ProGln: 2.807 ± 0.038	3.654ProArg: 3.654 ± 0.033	6.783ProSer: 6.783 ± 0.061	4.599ProThr: 4.599 ± 0.04	3.985ProVal: 3.985 ± 0.046	0.708ProTrp: 0.708 ± 0.012	1.525ProTyr: 1.525 ± 0.021	0.0ProXaa: 0.0 ± 0.0
Gln	3.61GlnAla: 3.61 ± 0.037	0.426GlnCys: 0.426 ± 0.01	2.015GlnAsp: 2.015 ± 0.02	2.635GlnGlu: 2.635 ± 0.032	1.225GlnPhe: 1.225 ± 0.014	2.542GlnGly: 2.542 ± 0.027	1.319GlnHis: 1.319 ± 0.024	1.737GlnIle: 1.737 ± 0.019	2.093GlnLys: 2.093 ± 0.025	3.343GlnLeu: 3.343 ± 0.028	0.892GlnMet: 0.892 ± 0.015	1.502GlnAsn: 1.502 ± 0.02	3.089GlnPro: 3.089 ± 0.04	4.343GlnGln: 4.343 ± 0.114	2.738GlnArg: 2.738 ± 0.03	3.079GlnSer: 3.079 ± 0.028	2.357GlnThr: 2.357 ± 0.023	2.208GlnVal: 2.208 ± 0.024	0.518GlnTrp: 0.518 ± 0.01	1.116GlnTyr: 1.116 ± 0.014	0.0GlnXaa: 0.0 ± 0.0
Arg	4.687ArgAla: 4.687 ± 0.035	0.665ArgCys: 0.665 ± 0.011	3.424ArgAsp: 3.424 ± 0.039	4.147ArgGlu: 4.147 ± 0.042	2.034ArgPhe: 2.034 ± 0.021	4.01ArgGly: 4.01 ± 0.036	1.627ArgHis: 1.627 ± 0.021	2.662ArgIle: 2.662 ± 0.028	3.586ArgLys: 3.586 ± 0.029	5.206ArgLeu: 5.206 ± 0.036	1.326ArgMet: 1.326 ± 0.016	2.265ArgAsn: 2.265 ± 0.022	3.805ArgPro: 3.805 ± 0.033	2.749ArgGln: 2.749 ± 0.026	5.331ArgArg: 5.331 ± 0.049	4.934ArgSer: 4.934 ± 0.049	3.287ArgThr: 3.287 ± 0.027	3.268ArgVal: 3.268 ± 0.028	0.871ArgTrp: 0.871 ± 0.013	1.602ArgTyr: 1.602 ± 0.021	0.0ArgXaa: 0.0 ± 0.0
Ser	6.822SerAla: 6.822 ± 0.047	0.792SerCys: 0.792 ± 0.015	4.18SerAsp: 4.18 ± 0.04	4.079SerGlu: 4.079 ± 0.033	2.779SerPhe: 2.779 ± 0.029	5.903SerGly: 5.903 ± 0.051	2.026SerHis: 2.026 ± 0.023	3.557SerIle: 3.557 ± 0.03	3.647SerLys: 3.647 ± 0.035	6.803SerLeu: 6.803 ± 0.043	1.631SerMet: 1.631 ± 0.02	3.087SerAsn: 3.087 ± 0.031	6.217SerPro: 6.217 ± 0.084	3.368SerGln: 3.368 ± 0.033	5.068SerArg: 5.068 ± 0.044	10.257SerSer: 10.257 ± 0.094	5.939SerThr: 5.939 ± 0.043	4.514SerVal: 4.514 ± 0.036	1.017SerTrp: 1.017 ± 0.016	1.919SerTyr: 1.919 ± 0.023	0.0SerXaa: 0.0 ± 0.0
Thr	5.538ThrAla: 5.538 ± 0.037	0.697ThrCys: 0.697 ± 0.015	2.813ThrAsp: 2.813 ± 0.024	3.232ThrGlu: 3.232 ± 0.031	2.038ThrPhe: 2.038 ± 0.023	4.318ThrGly: 4.318 ± 0.036	1.307ThrHis: 1.307 ± 0.016	2.934ThrIle: 2.934 ± 0.024	2.808ThrLys: 2.808 ± 0.026	4.914ThrLeu: 4.914 ± 0.034	1.184ThrMet: 1.184 ± 0.019	2.174ThrAsn: 2.174 ± 0.025	4.857ThrPro: 4.857 ± 0.045	2.178ThrGln: 2.178 ± 0.02	3.194ThrArg: 3.194 ± 0.029	5.745ThrSer: 5.745 ± 0.043	5.566ThrThr: 5.566 ± 0.06	3.673ThrVal: 3.673 ± 0.032	0.759ThrTrp: 0.759 ± 0.014	1.452ThrTyr: 1.452 ± 0.019	0.0ThrXaa: 0.0 ± 0.0
Val	5.182ValAla: 5.182 ± 0.037	0.753ValCys: 0.753 ± 0.011	3.683ValAsp: 3.683 ± 0.032	4.086ValGlu: 4.086 ± 0.044	2.174ValPhe: 2.174 ± 0.023	3.902ValGly: 3.902 ± 0.034	1.345ValHis: 1.345 ± 0.015	2.718ValIle: 2.718 ± 0.024	2.989ValLys: 2.989 ± 0.028	5.089ValLeu: 5.089 ± 0.037	1.314ValMet: 1.314 ± 0.018	2.161ValAsn: 2.161 ± 0.023	3.906ValPro: 3.906 ± 0.043	2.281ValGln: 2.281 ± 0.025	3.404ValArg: 3.404 ± 0.025	4.494ValSer: 4.494 ± 0.033	3.521ValThr: 3.521 ± 0.031	4.288ValVal: 4.288 ± 0.04	0.825ValTrp: 0.825 ± 0.011	1.579ValTyr: 1.579 ± 0.02	0.0ValXaa: 0.0 ± 0.0
Trp	1.042TrpAla: 1.042 ± 0.015	0.197TrpCys: 0.197 ± 0.006	0.839TrpAsp: 0.839 ± 0.013	0.855TrpGlu: 0.855 ± 0.013	0.493TrpPhe: 0.493 ± 0.011	0.895TrpGly: 0.895 ± 0.015	0.331TrpHis: 0.331 ± 0.008	0.637TrpIle: 0.637 ± 0.011	0.784TrpLys: 0.784 ± 0.015	1.208TrpLeu: 1.208 ± 0.017	0.353TrpMet: 0.353 ± 0.009	0.569TrpAsn: 0.569 ± 0.012	0.568TrpPro: 0.568 ± 0.012	0.512TrpGln: 0.512 ± 0.011	0.894TrpArg: 0.894 ± 0.015	0.939TrpSer: 0.939 ± 0.016	0.84TrpThr: 0.84 ± 0.012	0.824TrpVal: 0.824 ± 0.015	0.264TrpTrp: 0.264 ± 0.007	0.392TrpTyr: 0.392 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.96TyrAla: 1.96 ± 0.02	0.36TyrCys: 0.36 ± 0.009	1.565TyrAsp: 1.565 ± 0.019	1.477TyrGlu: 1.477 ± 0.019	1.051TyrPhe: 1.051 ± 0.015	1.994TyrGly: 1.994 ± 0.025	0.76TyrHis: 0.76 ± 0.011	1.184TyrIle: 1.184 ± 0.017	1.097TyrLys: 1.097 ± 0.016	2.414TyrLeu: 2.414 ± 0.025	0.562TyrMet: 0.562 ± 0.011	1.066TyrAsn: 1.066 ± 0.014	1.459TyrPro: 1.459 ± 0.019	1.085TyrGln: 1.085 ± 0.013	1.55TyrArg: 1.55 ± 0.02	1.864TyrSer: 1.864 ± 0.021	1.495TyrThr: 1.495 ± 0.019	1.466TyrVal: 1.466 ± 0.019	0.38TyrTrp: 0.38 ± 0.008	0.855TyrTyr: 0.855 ± 0.012	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 10267 proteins (5305852 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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