Tobacco necrosis virus (strain D) (TNV-D)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Betanecrovirus

Average proteome isoelectric point is 7.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O41350|O41350_TNVD 7 kDa protein OS=Tobacco necrosis virus (strain D) OX=12056 PE=4 SV=1
MM1 pKa = 7.54EE2 pKa = 4.64NSEE5 pKa = 4.18NVRR8 pKa = 11.84SGRR11 pKa = 11.84QNRR14 pKa = 11.84EE15 pKa = 3.42YY16 pKa = 11.11SRR18 pKa = 11.84DD19 pKa = 3.53RR20 pKa = 11.84QQEE23 pKa = 3.88GGYY26 pKa = 10.15KK27 pKa = 9.59EE28 pKa = 3.77ISKK31 pKa = 10.45AAVRR35 pKa = 11.84KK36 pKa = 10.06EE37 pKa = 3.77GDD39 pKa = 3.58VKK41 pKa = 11.06QDD43 pKa = 3.29MGPSVSMTVVGEE55 pKa = 4.19KK56 pKa = 10.73VEE58 pKa = 4.53FTQHH62 pKa = 4.45FHH64 pKa = 6.38FF65 pKa = 5.57

Molecular weight: 7.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O41349|O41349_TNVD 7 kDa protein OS=Tobacco necrosis virus (strain D) OX=12056 PE=4 SV=1
MM1 pKa = 7.2KK2 pKa = 9.47THH4 pKa = 6.06QFHH7 pKa = 7.07LKK9 pKa = 9.89IEE11 pKa = 4.34SRR13 pKa = 11.84WKK15 pKa = 8.12ILKK18 pKa = 9.52MSVVVGKK25 pKa = 8.42TEE27 pKa = 4.42SIVGIGSRR35 pKa = 11.84RR36 pKa = 11.84AAIRR40 pKa = 11.84KK41 pKa = 7.87LARR44 pKa = 11.84LPCVKK49 pKa = 10.14RR50 pKa = 11.84VTLSKK55 pKa = 10.2IWVHH59 pKa = 5.6QCLL62 pKa = 3.64

Molecular weight: 7.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1388

62

725

231.3

25.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.205 ± 1.195

2.089 ± 0.387

4.035 ± 0.552

6.052 ± 0.845

4.251 ± 0.38

6.124 ± 0.634

2.089 ± 0.491

5.908 ± 1.349

5.98 ± 0.568

7.781 ± 1.374

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.17 ± 0.297

3.242 ± 0.574

5.115 ± 0.511

3.602 ± 0.755

6.988 ± 0.792

7.493 ± 0.674

5.764 ± 0.805

8.718 ± 0.537

1.585 ± 0.181

2.738 ± 0.339
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski