Amino acid dipepetide frequency for Mycobacterium phage Validus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.112AlaAla: 22.112 ± 1.511
1.169AlaCys: 1.169 ± 0.232
8.946AlaAsp: 8.946 ± 0.843
10.776AlaGlu: 10.776 ± 1.052
2.694AlaPhe: 2.694 ± 0.585
9.505AlaGly: 9.505 ± 0.924
3.202AlaHis: 3.202 ± 0.403
5.083AlaIle: 5.083 ± 0.472
3.406AlaLys: 3.406 ± 0.447
11.386AlaLeu: 11.386 ± 0.658
3.66AlaMet: 3.66 ± 0.389
2.542AlaAsn: 2.542 ± 0.428
8.031AlaPro: 8.031 ± 0.698
5.032AlaGln: 5.032 ± 0.632
8.489AlaArg: 8.489 ± 0.791
5.846AlaSer: 5.846 ± 0.777
6.71AlaThr: 6.71 ± 0.809
11.589AlaVal: 11.589 ± 0.983
2.186AlaTrp: 2.186 ± 0.29
2.592AlaTyr: 2.592 ± 0.395
0.0AlaXaa: 0.0 ± 0.0
Cys
1.22CysAla: 1.22 ± 0.277
0.152CysCys: 0.152 ± 0.081
0.559CysAsp: 0.559 ± 0.148
0.915CysGlu: 0.915 ± 0.284
0.203CysPhe: 0.203 ± 0.09
1.423CysGly: 1.423 ± 0.325
0.305CysHis: 0.305 ± 0.139
0.356CysIle: 0.356 ± 0.135
0.305CysLys: 0.305 ± 0.133
0.559CysLeu: 0.559 ± 0.175
0.254CysMet: 0.254 ± 0.122
0.356CysAsn: 0.356 ± 0.12
1.067CysPro: 1.067 ± 0.264
0.407CysGln: 0.407 ± 0.127
0.864CysArg: 0.864 ± 0.229
0.915CysSer: 0.915 ± 0.238
0.407CysThr: 0.407 ± 0.137
0.661CysVal: 0.661 ± 0.184
0.457CysTrp: 0.457 ± 0.158
0.152CysTyr: 0.152 ± 0.08
0.0CysXaa: 0.0 ± 0.0
Asp
8.59AspAla: 8.59 ± 0.79
0.966AspCys: 0.966 ± 0.253
5.337AspAsp: 5.337 ± 0.736
6.049AspGlu: 6.049 ± 0.596
1.423AspPhe: 1.423 ± 0.292
5.693AspGly: 5.693 ± 0.455
1.169AspHis: 1.169 ± 0.274
1.322AspIle: 1.322 ± 0.259
1.83AspLys: 1.83 ± 0.316
5.642AspLeu: 5.642 ± 0.679
1.576AspMet: 1.576 ± 0.339
1.728AspAsn: 1.728 ± 0.268
4.321AspPro: 4.321 ± 0.498
2.033AspGln: 2.033 ± 0.305
5.083AspArg: 5.083 ± 0.651
2.694AspSer: 2.694 ± 0.271
3.101AspThr: 3.101 ± 0.383
3.812AspVal: 3.812 ± 0.433
0.966AspTrp: 0.966 ± 0.235
1.372AspTyr: 1.372 ± 0.27
0.0AspXaa: 0.0 ± 0.0
Glu
7.675GluAla: 7.675 ± 0.84
0.712GluCys: 0.712 ± 0.199
3.101GluAsp: 3.101 ± 0.49
0.915GluGlu: 0.915 ± 0.209
1.83GluPhe: 1.83 ± 0.322
4.778GluGly: 4.778 ± 0.465
2.084GluHis: 2.084 ± 0.33
2.033GluIle: 2.033 ± 0.324
1.474GluLys: 1.474 ± 0.323
6.405GluLeu: 6.405 ± 0.632
1.423GluMet: 1.423 ± 0.291
1.118GluAsn: 1.118 ± 0.227
2.948GluPro: 2.948 ± 0.479
2.542GluGln: 2.542 ± 0.46
5.185GluArg: 5.185 ± 0.598
2.389GluSer: 2.389 ± 0.317
2.999GluThr: 2.999 ± 0.402
5.744GluVal: 5.744 ± 0.695
0.966GluTrp: 0.966 ± 0.268
2.237GluTyr: 2.237 ± 0.365
0.0GluXaa: 0.0 ± 0.0
Phe
2.592PheAla: 2.592 ± 0.463
0.203PheCys: 0.203 ± 0.127
3.152PheAsp: 3.152 ± 0.423
1.271PheGlu: 1.271 ± 0.239
0.61PhePhe: 0.61 ± 0.202
2.745PheGly: 2.745 ± 0.353
0.356PheHis: 0.356 ± 0.121
1.017PheIle: 1.017 ± 0.185
1.017PheLys: 1.017 ± 0.205
1.932PheLeu: 1.932 ± 0.327
0.661PheMet: 0.661 ± 0.18
1.067PheAsn: 1.067 ± 0.321
1.067PhePro: 1.067 ± 0.229
0.762PheGln: 0.762 ± 0.208
1.576PheArg: 1.576 ± 0.254
1.271PheSer: 1.271 ± 0.287
1.627PheThr: 1.627 ± 0.28
2.491PheVal: 2.491 ± 0.346
0.407PheTrp: 0.407 ± 0.147
0.661PheTyr: 0.661 ± 0.215
0.0PheXaa: 0.0 ± 0.0
Gly
12.047GlyAla: 12.047 ± 1.248
1.322GlyCys: 1.322 ± 0.346
4.88GlyAsp: 4.88 ± 0.575
5.083GlyGlu: 5.083 ± 0.477
2.389GlyPhe: 2.389 ± 0.49
8.438GlyGly: 8.438 ± 1.182
1.932GlyHis: 1.932 ± 0.387
1.83GlyIle: 1.83 ± 0.401
3.202GlyLys: 3.202 ± 0.469
8.082GlyLeu: 8.082 ± 0.74
1.525GlyMet: 1.525 ± 0.246
2.745GlyAsn: 2.745 ± 0.342
3.863GlyPro: 3.863 ± 0.585
2.389GlyGln: 2.389 ± 0.463
6.456GlyArg: 6.456 ± 0.612
4.931GlySer: 4.931 ± 0.486
4.981GlyThr: 4.981 ± 0.429
7.116GlyVal: 7.116 ± 0.543
2.186GlyTrp: 2.186 ± 0.274
2.237GlyTyr: 2.237 ± 0.409
0.0GlyXaa: 0.0 ± 0.0
His
2.44HisAla: 2.44 ± 0.404
0.407HisCys: 0.407 ± 0.149
1.525HisAsp: 1.525 ± 0.345
1.576HisGlu: 1.576 ± 0.295
0.559HisPhe: 0.559 ± 0.156
2.186HisGly: 2.186 ± 0.416
0.508HisHis: 0.508 ± 0.166
0.864HisIle: 0.864 ± 0.217
0.661HisLys: 0.661 ± 0.207
1.982HisLeu: 1.982 ± 0.298
0.61HisMet: 0.61 ± 0.2
0.61HisAsn: 0.61 ± 0.176
1.423HisPro: 1.423 ± 0.269
0.457HisGln: 0.457 ± 0.178
1.83HisArg: 1.83 ± 0.341
1.017HisSer: 1.017 ± 0.25
1.423HisThr: 1.423 ± 0.242
2.033HisVal: 2.033 ± 0.377
0.559HisTrp: 0.559 ± 0.148
0.762HisTyr: 0.762 ± 0.198
0.0HisXaa: 0.0 ± 0.0
Ile
5.591IleAla: 5.591 ± 0.538
0.102IleCys: 0.102 ± 0.075
3.101IleAsp: 3.101 ± 0.449
2.897IleGlu: 2.897 ± 0.483
0.661IlePhe: 0.661 ± 0.214
3.558IleGly: 3.558 ± 0.596
0.508IleHis: 0.508 ± 0.178
0.61IleIle: 0.61 ± 0.157
1.271IleLys: 1.271 ± 0.255
2.592IleLeu: 2.592 ± 0.389
0.457IleMet: 0.457 ± 0.165
1.22IleAsn: 1.22 ± 0.248
2.135IlePro: 2.135 ± 0.357
0.813IleGln: 0.813 ± 0.151
2.694IleArg: 2.694 ± 0.355
1.677IleSer: 1.677 ± 0.322
2.186IleThr: 2.186 ± 0.302
2.948IleVal: 2.948 ± 0.5
0.762IleTrp: 0.762 ± 0.213
0.457IleTyr: 0.457 ± 0.197
0.0IleXaa: 0.0 ± 0.0
Lys
3.66LysAla: 3.66 ± 0.388
0.407LysCys: 0.407 ± 0.139
0.966LysAsp: 0.966 ± 0.202
0.559LysGlu: 0.559 ± 0.151
1.118LysPhe: 1.118 ± 0.212
2.237LysGly: 2.237 ± 0.418
0.712LysHis: 0.712 ± 0.245
1.423LysIle: 1.423 ± 0.376
0.508LysLys: 0.508 ± 0.149
2.847LysLeu: 2.847 ± 0.358
0.915LysMet: 0.915 ± 0.234
0.457LysAsn: 0.457 ± 0.195
2.237LysPro: 2.237 ± 0.418
0.508LysGln: 0.508 ± 0.164
3.05LysArg: 3.05 ± 0.448
0.966LysSer: 0.966 ± 0.188
1.932LysThr: 1.932 ± 0.329
2.796LysVal: 2.796 ± 0.352
0.712LysTrp: 0.712 ± 0.193
0.864LysTyr: 0.864 ± 0.233
0.0LysXaa: 0.0 ± 0.0
Leu
12.759LeuAla: 12.759 ± 0.941
0.61LeuCys: 0.61 ± 0.188
7.675LeuAsp: 7.675 ± 0.504
2.389LeuGlu: 2.389 ± 0.407
1.83LeuPhe: 1.83 ± 0.32
7.116LeuGly: 7.116 ± 0.718
1.779LeuHis: 1.779 ± 0.347
3.66LeuIle: 3.66 ± 0.411
2.186LeuLys: 2.186 ± 0.298
5.591LeuLeu: 5.591 ± 0.619
2.084LeuMet: 2.084 ± 0.325
2.135LeuAsn: 2.135 ± 0.315
4.524LeuPro: 4.524 ± 0.635
2.491LeuGln: 2.491 ± 0.358
7.015LeuArg: 7.015 ± 0.585
5.134LeuSer: 5.134 ± 0.529
5.388LeuThr: 5.388 ± 0.506
5.337LeuVal: 5.337 ± 0.387
1.372LeuTrp: 1.372 ± 0.236
1.576LeuTyr: 1.576 ± 0.285
0.0LeuXaa: 0.0 ± 0.0
Met
3.101MetAla: 3.101 ± 0.512
0.254MetCys: 0.254 ± 0.157
0.864MetAsp: 0.864 ± 0.244
0.813MetGlu: 0.813 ± 0.204
0.813MetPhe: 0.813 ± 0.199
1.982MetGly: 1.982 ± 0.352
0.457MetHis: 0.457 ± 0.139
0.864MetIle: 0.864 ± 0.238
0.356MetLys: 0.356 ± 0.136
1.677MetLeu: 1.677 ± 0.332
0.508MetMet: 0.508 ± 0.162
0.508MetAsn: 0.508 ± 0.179
1.576MetPro: 1.576 ± 0.236
0.712MetGln: 0.712 ± 0.173
1.627MetArg: 1.627 ± 0.291
2.44MetSer: 2.44 ± 0.33
1.677MetThr: 1.677 ± 0.272
1.932MetVal: 1.932 ± 0.361
0.254MetTrp: 0.254 ± 0.132
0.305MetTyr: 0.305 ± 0.146
0.0MetXaa: 0.0 ± 0.0
Asn
2.999AsnAla: 2.999 ± 0.455
0.356AsnCys: 0.356 ± 0.143
1.372AsnAsp: 1.372 ± 0.316
1.322AsnGlu: 1.322 ± 0.226
0.661AsnPhe: 0.661 ± 0.167
3.914AsnGly: 3.914 ± 0.537
0.457AsnHis: 0.457 ± 0.133
0.61AsnIle: 0.61 ± 0.171
0.864AsnLys: 0.864 ± 0.239
1.881AsnLeu: 1.881 ± 0.336
0.661AsnMet: 0.661 ± 0.178
0.457AsnAsn: 0.457 ± 0.196
2.287AsnPro: 2.287 ± 0.309
0.457AsnGln: 0.457 ± 0.165
1.322AsnArg: 1.322 ± 0.231
1.169AsnSer: 1.169 ± 0.299
1.728AsnThr: 1.728 ± 0.272
1.982AsnVal: 1.982 ± 0.361
0.254AsnTrp: 0.254 ± 0.103
0.457AsnTyr: 0.457 ± 0.117
0.0AsnXaa: 0.0 ± 0.0
Pro
8.336ProAla: 8.336 ± 0.651
0.508ProCys: 0.508 ± 0.183
3.101ProAsp: 3.101 ± 0.401
4.371ProGlu: 4.371 ± 0.457
1.677ProPhe: 1.677 ± 0.279
6.303ProGly: 6.303 ± 0.519
1.322ProHis: 1.322 ± 0.285
2.033ProIle: 2.033 ± 0.368
1.83ProLys: 1.83 ± 0.257
4.422ProLeu: 4.422 ± 0.532
0.864ProMet: 0.864 ± 0.188
1.017ProAsn: 1.017 ± 0.316
3.558ProPro: 3.558 ± 0.477
1.932ProGln: 1.932 ± 0.433
3.762ProArg: 3.762 ± 0.462
2.694ProSer: 2.694 ± 0.467
3.711ProThr: 3.711 ± 0.419
5.032ProVal: 5.032 ± 0.546
1.118ProTrp: 1.118 ± 0.213
1.423ProTyr: 1.423 ± 0.264
0.0ProXaa: 0.0 ± 0.0
Gln
4.829GlnAla: 4.829 ± 0.522
0.203GlnCys: 0.203 ± 0.096
1.067GlnAsp: 1.067 ± 0.25
0.864GlnGlu: 0.864 ± 0.215
0.559GlnPhe: 0.559 ± 0.151
2.643GlnGly: 2.643 ± 0.405
1.017GlnHis: 1.017 ± 0.203
1.576GlnIle: 1.576 ± 0.292
0.864GlnLys: 0.864 ± 0.189
2.592GlnLeu: 2.592 ± 0.333
1.067GlnMet: 1.067 ± 0.229
0.813GlnAsn: 0.813 ± 0.211
2.389GlnPro: 2.389 ± 0.389
1.474GlnGln: 1.474 ± 0.243
2.745GlnArg: 2.745 ± 0.355
1.22GlnSer: 1.22 ± 0.22
1.576GlnThr: 1.576 ± 0.311
2.796GlnVal: 2.796 ± 0.39
0.661GlnTrp: 0.661 ± 0.178
1.22GlnTyr: 1.22 ± 0.211
0.0GlnXaa: 0.0 ± 0.0
Arg
8.184ArgAla: 8.184 ± 0.921
1.118ArgCys: 1.118 ± 0.271
4.524ArgAsp: 4.524 ± 0.508
5.185ArgGlu: 5.185 ± 0.569
2.592ArgPhe: 2.592 ± 0.451
4.981ArgGly: 4.981 ± 0.461
1.932ArgHis: 1.932 ± 0.341
3.406ArgIle: 3.406 ± 0.439
3.152ArgLys: 3.152 ± 0.391
5.947ArgLeu: 5.947 ± 0.694
2.186ArgMet: 2.186 ± 0.336
2.084ArgAsn: 2.084 ± 0.292
3.863ArgPro: 3.863 ± 0.595
2.948ArgGln: 2.948 ± 0.343
6.913ArgArg: 6.913 ± 0.829
3.711ArgSer: 3.711 ± 0.526
3.863ArgThr: 3.863 ± 0.566
5.337ArgVal: 5.337 ± 0.604
1.83ArgTrp: 1.83 ± 0.345
2.135ArgTyr: 2.135 ± 0.438
0.0ArgXaa: 0.0 ± 0.0
Ser
6.405SerAla: 6.405 ± 0.663
0.61SerCys: 0.61 ± 0.171
2.847SerAsp: 2.847 ± 0.346
2.592SerGlu: 2.592 ± 0.363
1.881SerPhe: 1.881 ± 0.321
4.575SerGly: 4.575 ± 0.59
1.423SerHis: 1.423 ± 0.285
1.982SerIle: 1.982 ± 0.337
1.118SerLys: 1.118 ± 0.228
3.558SerLeu: 3.558 ± 0.329
1.22SerMet: 1.22 ± 0.26
1.474SerAsn: 1.474 ± 0.223
3.101SerPro: 3.101 ± 0.317
1.525SerGln: 1.525 ± 0.268
3.202SerArg: 3.202 ± 0.398
2.948SerSer: 2.948 ± 0.378
3.152SerThr: 3.152 ± 0.442
3.711SerVal: 3.711 ± 0.477
1.169SerTrp: 1.169 ± 0.235
1.067SerTyr: 1.067 ± 0.233
0.0SerXaa: 0.0 ± 0.0
Thr
6.964ThrAla: 6.964 ± 0.76
0.661ThrCys: 0.661 ± 0.168
3.558ThrAsp: 3.558 ± 0.383
3.152ThrGlu: 3.152 ± 0.409
2.338ThrPhe: 2.338 ± 0.323
4.931ThrGly: 4.931 ± 0.555
1.169ThrHis: 1.169 ± 0.271
3.152ThrIle: 3.152 ± 0.34
1.576ThrLys: 1.576 ± 0.279
4.27ThrLeu: 4.27 ± 0.47
0.915ThrMet: 0.915 ± 0.209
1.22ThrAsn: 1.22 ± 0.279
4.066ThrPro: 4.066 ± 0.566
1.525ThrGln: 1.525 ± 0.326
3.507ThrArg: 3.507 ± 0.386
2.999ThrSer: 2.999 ± 0.403
3.863ThrThr: 3.863 ± 0.426
4.524ThrVal: 4.524 ± 0.477
1.474ThrTrp: 1.474 ± 0.246
1.322ThrTyr: 1.322 ± 0.234
0.0ThrXaa: 0.0 ± 0.0
Val
11.03ValAla: 11.03 ± 0.866
0.966ValCys: 0.966 ± 0.189
5.439ValAsp: 5.439 ± 0.507
5.693ValGlu: 5.693 ± 0.633
1.83ValPhe: 1.83 ± 0.361
6.659ValGly: 6.659 ± 0.714
2.338ValHis: 2.338 ± 0.444
3.05ValIle: 3.05 ± 0.365
2.491ValLys: 2.491 ± 0.377
6.761ValLeu: 6.761 ± 0.657
1.474ValMet: 1.474 ± 0.243
2.237ValAsn: 2.237 ± 0.352
5.083ValPro: 5.083 ± 0.465
1.728ValGln: 1.728 ± 0.285
5.693ValArg: 5.693 ± 0.746
3.253ValSer: 3.253 ± 0.421
4.168ValThr: 4.168 ± 0.48
5.896ValVal: 5.896 ± 0.71
2.084ValTrp: 2.084 ± 0.345
1.322ValTyr: 1.322 ± 0.238
0.0ValXaa: 0.0 ± 0.0
Trp
2.338TrpAla: 2.338 ± 0.403
0.305TrpCys: 0.305 ± 0.126
0.661TrpAsp: 0.661 ± 0.202
0.762TrpGlu: 0.762 ± 0.262
0.407TrpPhe: 0.407 ± 0.152
1.576TrpGly: 1.576 ± 0.228
0.559TrpHis: 0.559 ± 0.189
0.813TrpIle: 0.813 ± 0.191
0.203TrpLys: 0.203 ± 0.097
2.796TrpLeu: 2.796 ± 0.417
0.356TrpMet: 0.356 ± 0.147
0.661TrpAsn: 0.661 ± 0.194
0.61TrpPro: 0.61 ± 0.161
1.525TrpGln: 1.525 ± 0.276
2.491TrpArg: 2.491 ± 0.341
1.017TrpSer: 1.017 ± 0.229
0.966TrpThr: 0.966 ± 0.207
1.423TrpVal: 1.423 ± 0.206
0.508TrpTrp: 0.508 ± 0.159
0.61TrpTyr: 0.61 ± 0.155
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.135TyrAla: 2.135 ± 0.314
0.508TyrCys: 0.508 ± 0.15
2.033TyrAsp: 2.033 ± 0.324
1.372TyrGlu: 1.372 ± 0.265
0.407TyrPhe: 0.407 ± 0.123
2.389TyrGly: 2.389 ± 0.309
0.203TyrHis: 0.203 ± 0.104
0.559TyrIle: 0.559 ± 0.183
0.559TyrLys: 0.559 ± 0.172
1.728TyrLeu: 1.728 ± 0.31
0.254TyrMet: 0.254 ± 0.1
0.762TyrAsn: 0.762 ± 0.208
0.864TyrPro: 0.864 ± 0.215
0.915TyrGln: 0.915 ± 0.264
2.389TyrArg: 2.389 ± 0.328
1.271TyrSer: 1.271 ± 0.267
1.728TyrThr: 1.728 ± 0.306
2.033TyrVal: 2.033 ± 0.409
0.661TyrTrp: 0.661 ± 0.21
0.61TyrTyr: 0.61 ± 0.229
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 105 proteins (19674 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski