Amino acid dipepetide frequency for Mycobacterium phage Jaan

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.11AlaAla: 11.11 ± 1.115
0.366AlaCys: 0.366 ± 0.156
6.105AlaAsp: 6.105 ± 0.656
7.02AlaGlu: 7.02 ± 0.803
3.48AlaPhe: 3.48 ± 0.508
8.424AlaGly: 8.424 ± 0.754
1.709AlaHis: 1.709 ± 0.286
4.151AlaIle: 4.151 ± 0.571
5.372AlaLys: 5.372 ± 0.691
8.73AlaLeu: 8.73 ± 0.819
2.503AlaMet: 2.503 ± 0.44
2.381AlaAsn: 2.381 ± 0.36
4.945AlaPro: 4.945 ± 0.789
3.541AlaGln: 3.541 ± 0.653
7.326AlaArg: 7.326 ± 0.787
4.273AlaSer: 4.273 ± 0.609
5.128AlaThr: 5.128 ± 0.607
7.57AlaVal: 7.57 ± 0.695
1.831AlaTrp: 1.831 ± 0.331
2.808AlaTyr: 2.808 ± 0.429
0.0AlaXaa: 0.0 ± 0.0
Cys
0.549CysAla: 0.549 ± 0.167
0.061CysCys: 0.061 ± 0.063
0.427CysAsp: 0.427 ± 0.156
0.366CysGlu: 0.366 ± 0.149
0.366CysPhe: 0.366 ± 0.151
0.916CysGly: 0.916 ± 0.241
0.244CysHis: 0.244 ± 0.104
0.061CysIle: 0.061 ± 0.068
0.488CysLys: 0.488 ± 0.174
0.549CysLeu: 0.549 ± 0.181
0.122CysMet: 0.122 ± 0.085
0.488CysAsn: 0.488 ± 0.152
0.549CysPro: 0.549 ± 0.184
0.244CysGln: 0.244 ± 0.126
0.488CysArg: 0.488 ± 0.178
0.427CysSer: 0.427 ± 0.187
0.733CysThr: 0.733 ± 0.241
0.427CysVal: 0.427 ± 0.146
0.549CysTrp: 0.549 ± 0.186
0.366CysTyr: 0.366 ± 0.153
0.0CysXaa: 0.0 ± 0.0
Asp
6.715AspAla: 6.715 ± 0.725
0.427AspCys: 0.427 ± 0.18
3.663AspAsp: 3.663 ± 0.464
4.334AspGlu: 4.334 ± 0.602
3.052AspPhe: 3.052 ± 0.54
5.983AspGly: 5.983 ± 0.682
1.648AspHis: 1.648 ± 0.345
3.602AspIle: 3.602 ± 0.421
2.686AspLys: 2.686 ± 0.395
5.616AspLeu: 5.616 ± 0.604
1.343AspMet: 1.343 ± 0.276
1.77AspAsn: 1.77 ± 0.32
4.578AspPro: 4.578 ± 0.498
1.526AspGln: 1.526 ± 0.287
2.503AspArg: 2.503 ± 0.368
3.663AspSer: 3.663 ± 0.393
3.113AspThr: 3.113 ± 0.435
3.846AspVal: 3.846 ± 0.441
1.343AspTrp: 1.343 ± 0.29
1.831AspTyr: 1.831 ± 0.282
0.0AspXaa: 0.0 ± 0.0
Glu
7.265GluAla: 7.265 ± 0.893
0.305GluCys: 0.305 ± 0.118
4.945GluAsp: 4.945 ± 0.652
5.006GluGlu: 5.006 ± 0.656
2.625GluPhe: 2.625 ± 0.448
5.128GluGly: 5.128 ± 0.617
2.076GluHis: 2.076 ± 0.378
3.846GluIle: 3.846 ± 0.405
2.869GluLys: 2.869 ± 0.565
6.349GluLeu: 6.349 ± 0.763
2.869GluMet: 2.869 ± 0.448
2.32GluAsn: 2.32 ± 0.341
2.747GluPro: 2.747 ± 0.412
2.503GluGln: 2.503 ± 0.359
4.64GluArg: 4.64 ± 0.492
2.198GluSer: 2.198 ± 0.337
3.235GluThr: 3.235 ± 0.392
3.968GluVal: 3.968 ± 0.53
1.404GluTrp: 1.404 ± 0.331
1.831GluTyr: 1.831 ± 0.301
0.0GluXaa: 0.0 ± 0.0
Phe
3.297PheAla: 3.297 ± 0.472
0.366PheCys: 0.366 ± 0.14
2.259PheAsp: 2.259 ± 0.392
3.052PheGlu: 3.052 ± 0.409
0.916PhePhe: 0.916 ± 0.291
2.808PheGly: 2.808 ± 0.414
0.733PheHis: 0.733 ± 0.245
1.282PheIle: 1.282 ± 0.274
1.404PheLys: 1.404 ± 0.259
2.259PheLeu: 2.259 ± 0.444
0.488PheMet: 0.488 ± 0.18
1.953PheAsn: 1.953 ± 0.335
1.953PhePro: 1.953 ± 0.33
0.855PheGln: 0.855 ± 0.286
2.076PheArg: 2.076 ± 0.367
1.953PheSer: 1.953 ± 0.385
2.381PheThr: 2.381 ± 0.422
2.93PheVal: 2.93 ± 0.5
0.488PheTrp: 0.488 ± 0.173
0.855PheTyr: 0.855 ± 0.209
0.0PheXaa: 0.0 ± 0.0
Gly
7.02GlyAla: 7.02 ± 0.769
0.733GlyCys: 0.733 ± 0.215
5.921GlyAsp: 5.921 ± 0.879
5.799GlyGlu: 5.799 ± 0.555
3.358GlyPhe: 3.358 ± 0.456
9.157GlyGly: 9.157 ± 1.564
1.831GlyHis: 1.831 ± 0.406
4.212GlyIle: 4.212 ± 0.879
3.663GlyLys: 3.663 ± 0.455
6.837GlyLeu: 6.837 ± 0.748
1.77GlyMet: 1.77 ± 0.309
3.48GlyAsn: 3.48 ± 0.664
4.823GlyPro: 4.823 ± 1.744
3.724GlyGln: 3.724 ± 0.446
4.701GlyArg: 4.701 ± 0.457
4.517GlySer: 4.517 ± 0.683
5.006GlyThr: 5.006 ± 0.555
6.044GlyVal: 6.044 ± 0.596
2.198GlyTrp: 2.198 ± 0.328
2.442GlyTyr: 2.442 ± 0.344
0.0GlyXaa: 0.0 ± 0.0
His
2.015HisAla: 2.015 ± 0.355
0.305HisCys: 0.305 ± 0.12
1.404HisAsp: 1.404 ± 0.307
1.77HisGlu: 1.77 ± 0.305
0.794HisPhe: 0.794 ± 0.204
1.892HisGly: 1.892 ± 0.391
0.61HisHis: 0.61 ± 0.201
1.099HisIle: 1.099 ± 0.28
0.916HisLys: 0.916 ± 0.263
1.404HisLeu: 1.404 ± 0.307
0.366HisMet: 0.366 ± 0.128
0.488HisAsn: 0.488 ± 0.159
1.038HisPro: 1.038 ± 0.235
0.916HisGln: 0.916 ± 0.205
1.892HisArg: 1.892 ± 0.394
0.977HisSer: 0.977 ± 0.275
0.855HisThr: 0.855 ± 0.181
1.221HisVal: 1.221 ± 0.318
0.488HisTrp: 0.488 ± 0.178
0.794HisTyr: 0.794 ± 0.252
0.0HisXaa: 0.0 ± 0.0
Ile
5.433IleAla: 5.433 ± 0.59
0.488IleCys: 0.488 ± 0.176
3.846IleAsp: 3.846 ± 0.36
4.64IleGlu: 4.64 ± 0.58
1.404IlePhe: 1.404 ± 0.278
4.029IleGly: 4.029 ± 0.442
1.038IleHis: 1.038 ± 0.274
2.076IleIle: 2.076 ± 0.353
2.442IleLys: 2.442 ± 0.425
4.212IleLeu: 4.212 ± 0.458
0.549IleMet: 0.549 ± 0.195
2.015IleAsn: 2.015 ± 0.33
3.113IlePro: 3.113 ± 0.467
1.465IleGln: 1.465 ± 0.335
3.419IleArg: 3.419 ± 0.443
2.076IleSer: 2.076 ± 0.323
3.541IleThr: 3.541 ± 0.385
3.663IleVal: 3.663 ± 0.487
0.855IleTrp: 0.855 ± 0.288
0.916IleTyr: 0.916 ± 0.204
0.0IleXaa: 0.0 ± 0.0
Lys
4.823LysAla: 4.823 ± 0.59
0.305LysCys: 0.305 ± 0.13
2.564LysAsp: 2.564 ± 0.447
2.442LysGlu: 2.442 ± 0.505
1.038LysPhe: 1.038 ± 0.247
4.09LysGly: 4.09 ± 0.681
0.977LysHis: 0.977 ± 0.245
3.052LysIle: 3.052 ± 0.496
3.174LysLys: 3.174 ± 0.595
3.907LysLeu: 3.907 ± 0.567
0.855LysMet: 0.855 ± 0.21
1.709LysAsn: 1.709 ± 0.353
3.663LysPro: 3.663 ± 0.604
1.709LysGln: 1.709 ± 0.37
3.663LysArg: 3.663 ± 0.497
2.808LysSer: 2.808 ± 0.47
2.503LysThr: 2.503 ± 0.418
3.541LysVal: 3.541 ± 0.469
0.916LysTrp: 0.916 ± 0.25
1.038LysTyr: 1.038 ± 0.248
0.0LysXaa: 0.0 ± 0.0
Leu
8.363LeuAla: 8.363 ± 0.789
0.733LeuCys: 0.733 ± 0.219
4.884LeuAsp: 4.884 ± 0.537
5.067LeuGlu: 5.067 ± 0.592
2.442LeuPhe: 2.442 ± 0.277
5.311LeuGly: 5.311 ± 0.456
1.709LeuHis: 1.709 ± 0.351
5.067LeuIle: 5.067 ± 0.578
4.334LeuLys: 4.334 ± 0.77
4.762LeuLeu: 4.762 ± 0.585
2.625LeuMet: 2.625 ± 0.381
2.32LeuAsn: 2.32 ± 0.404
4.884LeuPro: 4.884 ± 0.534
2.625LeuGln: 2.625 ± 0.385
5.921LeuArg: 5.921 ± 0.586
5.86LeuSer: 5.86 ± 0.525
4.762LeuThr: 4.762 ± 0.533
4.273LeuVal: 4.273 ± 0.496
1.404LeuTrp: 1.404 ± 0.27
2.564LeuTyr: 2.564 ± 0.406
0.0LeuXaa: 0.0 ± 0.0
Met
3.297MetAla: 3.297 ± 0.493
0.122MetCys: 0.122 ± 0.096
0.977MetAsp: 0.977 ± 0.218
1.099MetGlu: 1.099 ± 0.232
0.61MetPhe: 0.61 ± 0.166
2.32MetGly: 2.32 ± 0.366
0.733MetHis: 0.733 ± 0.179
1.77MetIle: 1.77 ± 0.366
1.099MetLys: 1.099 ± 0.302
1.77MetLeu: 1.77 ± 0.375
0.672MetMet: 0.672 ± 0.215
0.794MetAsn: 0.794 ± 0.221
1.16MetPro: 1.16 ± 0.238
0.61MetGln: 0.61 ± 0.185
1.221MetArg: 1.221 ± 0.297
2.381MetSer: 2.381 ± 0.363
1.831MetThr: 1.831 ± 0.325
1.404MetVal: 1.404 ± 0.284
0.366MetTrp: 0.366 ± 0.133
0.794MetTyr: 0.794 ± 0.219
0.0MetXaa: 0.0 ± 0.0
Asn
3.541AsnAla: 3.541 ± 0.455
0.305AsnCys: 0.305 ± 0.157
1.526AsnAsp: 1.526 ± 0.291
2.503AsnGlu: 2.503 ± 0.336
0.916AsnPhe: 0.916 ± 0.285
3.602AsnGly: 3.602 ± 0.58
0.794AsnHis: 0.794 ± 0.219
1.587AsnIle: 1.587 ± 0.329
1.282AsnLys: 1.282 ± 0.264
2.93AsnLeu: 2.93 ± 0.447
0.794AsnMet: 0.794 ± 0.177
0.855AsnAsn: 0.855 ± 0.235
2.503AsnPro: 2.503 ± 0.418
0.916AsnGln: 0.916 ± 0.235
2.076AsnArg: 2.076 ± 0.387
1.099AsnSer: 1.099 ± 0.196
1.526AsnThr: 1.526 ± 0.287
2.808AsnVal: 2.808 ± 0.438
0.794AsnTrp: 0.794 ± 0.213
1.038AsnTyr: 1.038 ± 0.247
0.0AsnXaa: 0.0 ± 0.0
Pro
5.311ProAla: 5.311 ± 0.65
0.427ProCys: 0.427 ± 0.2
4.029ProAsp: 4.029 ± 0.437
4.517ProGlu: 4.517 ± 0.597
2.137ProPhe: 2.137 ± 0.373
5.677ProGly: 5.677 ± 0.838
1.099ProHis: 1.099 ± 0.244
2.869ProIle: 2.869 ± 0.419
3.113ProLys: 3.113 ± 0.665
2.991ProLeu: 2.991 ± 0.474
1.221ProMet: 1.221 ± 0.281
2.137ProAsn: 2.137 ± 0.404
3.174ProPro: 3.174 ± 0.542
2.259ProGln: 2.259 ± 0.833
3.541ProArg: 3.541 ± 0.557
2.381ProSer: 2.381 ± 0.351
3.663ProThr: 3.663 ± 0.39
3.419ProVal: 3.419 ± 0.443
0.977ProTrp: 0.977 ± 0.318
1.587ProTyr: 1.587 ± 0.333
0.0ProXaa: 0.0 ± 0.0
Gln
4.395GlnAla: 4.395 ± 0.487
0.366GlnCys: 0.366 ± 0.134
1.465GlnAsp: 1.465 ± 0.284
2.625GlnGlu: 2.625 ± 0.384
1.282GlnPhe: 1.282 ± 0.269
3.602GlnGly: 3.602 ± 1.267
0.366GlnHis: 0.366 ± 0.155
2.32GlnIle: 2.32 ± 0.331
1.526GlnLys: 1.526 ± 0.296
2.625GlnLeu: 2.625 ± 0.378
0.733GlnMet: 0.733 ± 0.21
0.916GlnAsn: 0.916 ± 0.301
1.221GlnPro: 1.221 ± 0.248
1.709GlnGln: 1.709 ± 0.419
2.32GlnArg: 2.32 ± 0.4
1.465GlnSer: 1.465 ± 0.32
1.831GlnThr: 1.831 ± 0.389
2.564GlnVal: 2.564 ± 0.403
0.733GlnTrp: 0.733 ± 0.206
0.61GlnTyr: 0.61 ± 0.147
0.0GlnXaa: 0.0 ± 0.0
Arg
5.921ArgAla: 5.921 ± 0.687
0.672ArgCys: 0.672 ± 0.276
3.174ArgAsp: 3.174 ± 0.482
4.517ArgGlu: 4.517 ± 0.552
2.381ArgPhe: 2.381 ± 0.397
5.25ArgGly: 5.25 ± 0.776
1.953ArgHis: 1.953 ± 0.357
3.419ArgIle: 3.419 ± 0.435
3.968ArgLys: 3.968 ± 0.546
6.044ArgLeu: 6.044 ± 0.598
2.259ArgMet: 2.259 ± 0.389
1.831ArgAsn: 1.831 ± 0.335
2.503ArgPro: 2.503 ± 0.398
2.137ArgGln: 2.137 ± 0.353
5.372ArgArg: 5.372 ± 0.67
2.869ArgSer: 2.869 ± 0.398
2.442ArgThr: 2.442 ± 0.43
4.701ArgVal: 4.701 ± 0.605
1.465ArgTrp: 1.465 ± 0.301
2.381ArgTyr: 2.381 ± 0.394
0.0ArgXaa: 0.0 ± 0.0
Ser
3.968SerAla: 3.968 ± 0.469
0.427SerCys: 0.427 ± 0.156
3.602SerAsp: 3.602 ± 0.551
3.052SerGlu: 3.052 ± 0.379
2.015SerPhe: 2.015 ± 0.403
4.517SerGly: 4.517 ± 0.691
0.61SerHis: 0.61 ± 0.18
2.381SerIle: 2.381 ± 0.396
2.32SerLys: 2.32 ± 0.423
4.64SerLeu: 4.64 ± 0.566
1.587SerMet: 1.587 ± 0.361
1.282SerAsn: 1.282 ± 0.273
2.808SerPro: 2.808 ± 0.38
2.137SerGln: 2.137 ± 0.362
3.235SerArg: 3.235 ± 0.431
2.625SerSer: 2.625 ± 0.376
3.174SerThr: 3.174 ± 0.458
3.663SerVal: 3.663 ± 0.464
0.977SerTrp: 0.977 ± 0.242
1.709SerTyr: 1.709 ± 0.284
0.0SerXaa: 0.0 ± 0.0
Thr
5.128ThrAla: 5.128 ± 0.442
0.488ThrCys: 0.488 ± 0.152
3.541ThrAsp: 3.541 ± 0.441
2.93ThrGlu: 2.93 ± 0.346
2.076ThrPhe: 2.076 ± 0.379
5.616ThrGly: 5.616 ± 0.696
1.038ThrHis: 1.038 ± 0.264
2.198ThrIle: 2.198 ± 0.451
2.991ThrLys: 2.991 ± 0.393
5.006ThrLeu: 5.006 ± 0.553
1.709ThrMet: 1.709 ± 0.312
1.404ThrAsn: 1.404 ± 0.282
4.273ThrPro: 4.273 ± 0.581
2.259ThrGln: 2.259 ± 0.34
2.564ThrArg: 2.564 ± 0.376
2.198ThrSer: 2.198 ± 0.4
3.052ThrThr: 3.052 ± 0.515
4.395ThrVal: 4.395 ± 0.573
1.343ThrTrp: 1.343 ± 0.351
1.77ThrTyr: 1.77 ± 0.318
0.0ThrXaa: 0.0 ± 0.0
Val
6.227ValAla: 6.227 ± 0.742
0.855ValCys: 0.855 ± 0.196
5.555ValAsp: 5.555 ± 0.596
4.64ValGlu: 4.64 ± 0.574
2.015ValPhe: 2.015 ± 0.363
5.067ValGly: 5.067 ± 0.565
1.038ValHis: 1.038 ± 0.241
3.785ValIle: 3.785 ± 0.459
3.174ValLys: 3.174 ± 0.399
5.128ValLeu: 5.128 ± 0.631
1.282ValMet: 1.282 ± 0.291
3.052ValAsn: 3.052 ± 0.461
3.724ValPro: 3.724 ± 0.542
1.77ValGln: 1.77 ± 0.32
4.456ValArg: 4.456 ± 0.54
4.64ValSer: 4.64 ± 0.623
4.151ValThr: 4.151 ± 0.6
4.823ValVal: 4.823 ± 0.484
1.282ValTrp: 1.282 ± 0.244
1.709ValTyr: 1.709 ± 0.33
0.0ValXaa: 0.0 ± 0.0
Trp
1.648TrpAla: 1.648 ± 0.341
0.427TrpCys: 0.427 ± 0.159
1.343TrpAsp: 1.343 ± 0.268
0.916TrpGlu: 0.916 ± 0.248
0.672TrpPhe: 0.672 ± 0.21
1.343TrpGly: 1.343 ± 0.307
0.61TrpHis: 0.61 ± 0.223
1.038TrpIle: 1.038 ± 0.216
0.855TrpLys: 0.855 ± 0.221
1.77TrpLeu: 1.77 ± 0.317
0.672TrpMet: 0.672 ± 0.177
1.343TrpAsn: 1.343 ± 0.389
0.916TrpPro: 0.916 ± 0.215
0.794TrpGln: 0.794 ± 0.234
1.343TrpArg: 1.343 ± 0.242
0.916TrpSer: 0.916 ± 0.206
1.648TrpThr: 1.648 ± 0.28
1.038TrpVal: 1.038 ± 0.255
0.549TrpTrp: 0.549 ± 0.19
0.549TrpTyr: 0.549 ± 0.171
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.747TyrAla: 2.747 ± 0.438
0.244TyrCys: 0.244 ± 0.124
2.015TyrAsp: 2.015 ± 0.317
1.831TyrGlu: 1.831 ± 0.355
0.733TyrPhe: 0.733 ± 0.186
2.503TyrGly: 2.503 ± 0.333
0.427TyrHis: 0.427 ± 0.16
1.404TyrIle: 1.404 ± 0.253
1.099TyrLys: 1.099 ± 0.253
2.381TyrLeu: 2.381 ± 0.359
0.549TyrMet: 0.549 ± 0.201
0.855TyrAsn: 0.855 ± 0.181
1.953TyrPro: 1.953 ± 0.356
1.038TyrGln: 1.038 ± 0.196
2.503TyrArg: 2.503 ± 0.397
1.404TyrSer: 1.404 ± 0.303
1.404TyrThr: 1.404 ± 0.308
2.076TyrVal: 2.076 ± 0.373
0.427TyrTrp: 0.427 ± 0.158
0.794TyrTyr: 0.794 ± 0.237
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 90 proteins (16382 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski