Thermosporothrix hazakensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Ktedonobacteria; Ktedonobacterales; Thermosporotrichaceae; Thermosporothrix

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6382 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A326U852|A0A326U852_9CHLR Uncharacterized protein OS=Thermosporothrix hazakensis OX=644383 GN=EI42_03543 PE=4 SV=1
MM1 pKa = 6.91EE2 pKa = 3.89QALIEE7 pKa = 4.64LEE9 pKa = 4.13HH10 pKa = 6.63DD11 pKa = 3.85TPVEE15 pKa = 4.19DD16 pKa = 6.21DD17 pKa = 4.01FEE19 pKa = 6.7LDD21 pKa = 3.31IRR23 pKa = 11.84VSAPSSDD30 pKa = 3.31IQPVALATANTCYY43 pKa = 10.3KK44 pKa = 10.62SCGTTCLSVNEE55 pKa = 4.38EE56 pKa = 4.27GG57 pKa = 4.75

Molecular weight: 6.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A326TZZ1|A0A326TZZ1_9CHLR HD domain-containing protein OS=Thermosporothrix hazakensis OX=644383 GN=EI42_05132 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.45RR3 pKa = 11.84TWQPKK8 pKa = 8.26RR9 pKa = 11.84IPRR12 pKa = 11.84KK13 pKa = 9.21RR14 pKa = 11.84EE15 pKa = 3.41HH16 pKa = 7.12GFMKK20 pKa = 10.49RR21 pKa = 11.84MATRR25 pKa = 11.84NGRR28 pKa = 11.84RR29 pKa = 11.84VLKK32 pKa = 10.49ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.39GRR39 pKa = 11.84WKK41 pKa = 9.86LTVV44 pKa = 2.99

Molecular weight: 5.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6382

0

6382

2033648

22

3753

318.7

35.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.314 ± 0.032

1.121 ± 0.011

4.479 ± 0.022

6.613 ± 0.033

3.715 ± 0.019

6.906 ± 0.027

2.533 ± 0.016

5.609 ± 0.022

3.58 ± 0.025

11.535 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.234 ± 0.013

2.838 ± 0.018

5.258 ± 0.024

5.159 ± 0.024

6.604 ± 0.031

5.667 ± 0.024

5.621 ± 0.021

6.677 ± 0.026

1.438 ± 0.012

3.098 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski