Amino acid dipepetide frequency for Synechococcus phage S-MbCM100

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.949AlaAla: 5.949 ± 0.494
0.676AlaCys: 0.676 ± 0.135
4.598AlaAsp: 4.598 ± 0.34
3.903AlaGlu: 3.903 ± 0.342
3.059AlaPhe: 3.059 ± 0.224
6.775AlaGly: 6.775 ± 0.675
0.769AlaHis: 0.769 ± 0.133
3.96AlaIle: 3.96 ± 0.289
3.453AlaLys: 3.453 ± 0.346
4.617AlaLeu: 4.617 ± 0.329
1.332AlaMet: 1.332 ± 0.217
4.26AlaAsn: 4.26 ± 0.355
2.496AlaPro: 2.496 ± 0.221
2.44AlaGln: 2.44 ± 0.25
2.834AlaArg: 2.834 ± 0.303
5.705AlaSer: 5.705 ± 0.411
5.574AlaThr: 5.574 ± 0.461
4.054AlaVal: 4.054 ± 0.318
0.582AlaTrp: 0.582 ± 0.114
2.046AlaTyr: 2.046 ± 0.191
0.0AlaXaa: 0.0 ± 0.0
Cys
0.619CysAla: 0.619 ± 0.108
0.094CysCys: 0.094 ± 0.042
0.638CysAsp: 0.638 ± 0.131
0.488CysGlu: 0.488 ± 0.112
0.525CysPhe: 0.525 ± 0.109
0.544CysGly: 0.544 ± 0.105
0.225CysHis: 0.225 ± 0.065
0.525CysIle: 0.525 ± 0.098
0.657CysLys: 0.657 ± 0.118
0.638CysLeu: 0.638 ± 0.096
0.206CysMet: 0.206 ± 0.067
0.469CysAsn: 0.469 ± 0.098
0.338CysPro: 0.338 ± 0.07
0.375CysGln: 0.375 ± 0.091
0.319CysArg: 0.319 ± 0.091
0.601CysSer: 0.601 ± 0.111
0.544CysThr: 0.544 ± 0.092
0.582CysVal: 0.582 ± 0.121
0.094CysTrp: 0.094 ± 0.04
0.3CysTyr: 0.3 ± 0.068
0.0CysXaa: 0.0 ± 0.0
Asp
5.442AspAla: 5.442 ± 0.386
0.638AspCys: 0.638 ± 0.125
4.56AspAsp: 4.56 ± 0.407
3.96AspGlu: 3.96 ± 0.328
2.909AspPhe: 2.909 ± 0.244
6.625AspGly: 6.625 ± 0.419
0.92AspHis: 0.92 ± 0.155
4.185AspIle: 4.185 ± 0.34
3.153AspLys: 3.153 ± 0.347
5.104AspLeu: 5.104 ± 0.294
1.389AspMet: 1.389 ± 0.208
3.472AspAsn: 3.472 ± 0.262
3.021AspPro: 3.021 ± 0.263
1.989AspGln: 1.989 ± 0.199
2.214AspArg: 2.214 ± 0.212
4.523AspSer: 4.523 ± 0.269
4.936AspThr: 4.936 ± 0.41
4.241AspVal: 4.241 ± 0.249
1.088AspTrp: 1.088 ± 0.157
3.096AspTyr: 3.096 ± 0.274
0.0AspXaa: 0.0 ± 0.0
Glu
2.909GluAla: 2.909 ± 0.277
0.694GluCys: 0.694 ± 0.128
3.81GluAsp: 3.81 ± 0.28
4.222GluGlu: 4.222 ± 0.442
2.909GluPhe: 2.909 ± 0.285
3.697GluGly: 3.697 ± 0.322
0.732GluHis: 0.732 ± 0.144
4.204GluIle: 4.204 ± 0.277
3.415GluLys: 3.415 ± 0.384
4.954GluLeu: 4.954 ± 0.345
1.839GluMet: 1.839 ± 0.288
2.965GluAsn: 2.965 ± 0.274
1.614GluPro: 1.614 ± 0.16
2.139GluGln: 2.139 ± 0.212
2.533GluArg: 2.533 ± 0.252
4.466GluSer: 4.466 ± 0.36
4.016GluThr: 4.016 ± 0.279
4.26GluVal: 4.26 ± 0.292
0.788GluTrp: 0.788 ± 0.121
2.759GluTyr: 2.759 ± 0.208
0.0GluXaa: 0.0 ± 0.0
Phe
2.984PheAla: 2.984 ± 0.275
0.525PheCys: 0.525 ± 0.107
3.603PheAsp: 3.603 ± 0.314
2.552PheGlu: 2.552 ± 0.234
1.877PhePhe: 1.877 ± 0.187
2.984PheGly: 2.984 ± 0.257
0.488PheHis: 0.488 ± 0.106
2.89PheIle: 2.89 ± 0.264
2.44PheLys: 2.44 ± 0.281
2.909PheLeu: 2.909 ± 0.266
1.051PheMet: 1.051 ± 0.204
2.89PheAsn: 2.89 ± 0.223
1.37PhePro: 1.37 ± 0.194
1.764PheGln: 1.764 ± 0.171
1.614PheArg: 1.614 ± 0.189
3.734PheSer: 3.734 ± 0.259
3.228PheThr: 3.228 ± 0.247
2.383PheVal: 2.383 ± 0.229
0.357PheTrp: 0.357 ± 0.089
1.708PheTyr: 1.708 ± 0.16
0.0PheXaa: 0.0 ± 0.0
Gly
5.949GlyAla: 5.949 ± 0.503
0.544GlyCys: 0.544 ± 0.08
5.555GlyAsp: 5.555 ± 0.38
4.166GlyGlu: 4.166 ± 0.3
3.172GlyPhe: 3.172 ± 0.24
8.182GlyGly: 8.182 ± 1.283
0.92GlyHis: 0.92 ± 0.163
4.541GlyIle: 4.541 ± 0.289
3.547GlyLys: 3.547 ± 0.315
4.598GlyLeu: 4.598 ± 0.276
1.633GlyMet: 1.633 ± 0.266
4.016GlyAsn: 4.016 ± 0.312
2.027GlyPro: 2.027 ± 0.22
2.721GlyGln: 2.721 ± 0.246
2.965GlyArg: 2.965 ± 0.271
7.169GlySer: 7.169 ± 0.645
7.563GlyThr: 7.563 ± 0.602
5.086GlyVal: 5.086 ± 0.303
1.088GlyTrp: 1.088 ± 0.145
3.922GlyTyr: 3.922 ± 0.317
0.0GlyXaa: 0.0 ± 0.0
His
0.582HisAla: 0.582 ± 0.111
0.15HisCys: 0.15 ± 0.055
0.788HisAsp: 0.788 ± 0.12
0.826HisGlu: 0.826 ± 0.14
0.676HisPhe: 0.676 ± 0.134
0.938HisGly: 0.938 ± 0.155
0.244HisHis: 0.244 ± 0.078
0.863HisIle: 0.863 ± 0.113
0.788HisLys: 0.788 ± 0.155
0.92HisLeu: 0.92 ± 0.15
0.375HisMet: 0.375 ± 0.086
0.657HisAsn: 0.657 ± 0.133
0.826HisPro: 0.826 ± 0.13
0.394HisGln: 0.394 ± 0.086
0.507HisArg: 0.507 ± 0.1
0.882HisSer: 0.882 ± 0.136
0.882HisThr: 0.882 ± 0.14
0.957HisVal: 0.957 ± 0.16
0.15HisTrp: 0.15 ± 0.059
0.751HisTyr: 0.751 ± 0.135
0.0HisXaa: 0.0 ± 0.0
Ile
4.054IleAla: 4.054 ± 0.318
0.732IleCys: 0.732 ± 0.123
4.992IleAsp: 4.992 ± 0.345
4.222IleGlu: 4.222 ± 0.263
2.721IlePhe: 2.721 ± 0.233
4.673IleGly: 4.673 ± 0.349
0.619IleHis: 0.619 ± 0.119
3.791IleIle: 3.791 ± 0.295
3.997IleLys: 3.997 ± 0.361
4.598IleLeu: 4.598 ± 0.314
1.032IleMet: 1.032 ± 0.183
3.96IleAsn: 3.96 ± 0.287
2.777IlePro: 2.777 ± 0.252
2.74IleGln: 2.74 ± 0.203
2.327IleArg: 2.327 ± 0.257
4.992IleSer: 4.992 ± 0.401
5.499IleThr: 5.499 ± 0.492
4.147IleVal: 4.147 ± 0.3
0.601IleTrp: 0.601 ± 0.108
2.027IleTyr: 2.027 ± 0.211
0.0IleXaa: 0.0 ± 0.0
Lys
3.153LysAla: 3.153 ± 0.326
0.563LysCys: 0.563 ± 0.099
3.34LysAsp: 3.34 ± 0.299
3.772LysGlu: 3.772 ± 0.489
2.552LysPhe: 2.552 ± 0.245
3.491LysGly: 3.491 ± 0.274
0.92LysHis: 0.92 ± 0.156
3.828LysIle: 3.828 ± 0.339
4.335LysLys: 4.335 ± 0.598
4.485LysLeu: 4.485 ± 0.35
1.445LysMet: 1.445 ± 0.252
3.059LysAsn: 3.059 ± 0.311
1.82LysPro: 1.82 ± 0.216
1.933LysGln: 1.933 ± 0.235
2.289LysArg: 2.289 ± 0.231
3.791LysSer: 3.791 ± 0.286
3.472LysThr: 3.472 ± 0.259
3.903LysVal: 3.903 ± 0.257
0.657LysTrp: 0.657 ± 0.111
2.552LysTyr: 2.552 ± 0.282
0.0LysXaa: 0.0 ± 0.0
Leu
4.71LeuAla: 4.71 ± 0.392
0.732LeuCys: 0.732 ± 0.152
5.517LeuAsp: 5.517 ± 0.439
4.354LeuGlu: 4.354 ± 0.38
2.946LeuPhe: 2.946 ± 0.216
4.748LeuGly: 4.748 ± 0.452
1.145LeuHis: 1.145 ± 0.171
4.354LeuIle: 4.354 ± 0.31
4.504LeuLys: 4.504 ± 0.331
5.48LeuLeu: 5.48 ± 0.421
1.314LeuMet: 1.314 ± 0.202
4.485LeuAsn: 4.485 ± 0.296
3.021LeuPro: 3.021 ± 0.351
3.134LeuGln: 3.134 ± 0.227
3.247LeuArg: 3.247 ± 0.287
5.367LeuSer: 5.367 ± 0.292
5.517LeuThr: 5.517 ± 0.431
4.279LeuVal: 4.279 ± 0.335
0.751LeuTrp: 0.751 ± 0.147
3.04LeuTyr: 3.04 ± 0.252
0.0LeuXaa: 0.0 ± 0.0
Met
1.576MetAla: 1.576 ± 0.246
0.094MetCys: 0.094 ± 0.042
1.182MetAsp: 1.182 ± 0.189
1.107MetGlu: 1.107 ± 0.193
0.976MetPhe: 0.976 ± 0.158
1.182MetGly: 1.182 ± 0.204
0.432MetHis: 0.432 ± 0.102
1.332MetIle: 1.332 ± 0.21
1.464MetLys: 1.464 ± 0.232
1.689MetLeu: 1.689 ± 0.243
0.638MetMet: 0.638 ± 0.116
1.164MetAsn: 1.164 ± 0.18
0.844MetPro: 0.844 ± 0.131
0.938MetGln: 0.938 ± 0.18
0.844MetArg: 0.844 ± 0.15
1.501MetSer: 1.501 ± 0.212
1.689MetThr: 1.689 ± 0.284
0.901MetVal: 0.901 ± 0.142
0.3MetTrp: 0.3 ± 0.077
0.582MetTyr: 0.582 ± 0.111
0.0MetXaa: 0.0 ± 0.0
Asn
3.697AsnAla: 3.697 ± 0.279
0.45AsnCys: 0.45 ± 0.09
3.434AsnAsp: 3.434 ± 0.255
3.397AsnGlu: 3.397 ± 0.239
2.402AsnPhe: 2.402 ± 0.208
4.579AsnGly: 4.579 ± 0.351
0.694AsnHis: 0.694 ± 0.131
4.185AsnIle: 4.185 ± 0.268
2.928AsnLys: 2.928 ± 0.283
4.579AsnLeu: 4.579 ± 0.386
0.826AsnMet: 0.826 ± 0.17
3.453AsnAsn: 3.453 ± 0.338
3.153AsnPro: 3.153 ± 0.288
1.895AsnGln: 1.895 ± 0.167
2.327AsnArg: 2.327 ± 0.192
4.373AsnSer: 4.373 ± 0.381
4.091AsnThr: 4.091 ± 0.355
4.541AsnVal: 4.541 ± 0.285
0.638AsnTrp: 0.638 ± 0.123
2.383AsnTyr: 2.383 ± 0.222
0.0AsnXaa: 0.0 ± 0.0
Pro
2.759ProAla: 2.759 ± 0.237
0.338ProCys: 0.338 ± 0.096
2.458ProAsp: 2.458 ± 0.25
2.646ProGlu: 2.646 ± 0.229
1.614ProPhe: 1.614 ± 0.183
3.172ProGly: 3.172 ± 0.291
0.638ProHis: 0.638 ± 0.12
2.402ProIle: 2.402 ± 0.259
2.083ProLys: 2.083 ± 0.242
2.233ProLeu: 2.233 ± 0.251
0.638ProMet: 0.638 ± 0.154
2.365ProAsn: 2.365 ± 0.199
1.614ProPro: 1.614 ± 0.212
1.37ProGln: 1.37 ± 0.14
1.314ProArg: 1.314 ± 0.154
3.265ProSer: 3.265 ± 0.291
3.528ProThr: 3.528 ± 0.244
2.496ProVal: 2.496 ± 0.24
0.563ProTrp: 0.563 ± 0.099
1.445ProTyr: 1.445 ± 0.189
0.0ProXaa: 0.0 ± 0.0
Gln
2.027GlnAla: 2.027 ± 0.193
0.357GlnCys: 0.357 ± 0.083
2.158GlnAsp: 2.158 ± 0.195
2.346GlnGlu: 2.346 ± 0.231
1.614GlnPhe: 1.614 ± 0.156
2.44GlnGly: 2.44 ± 0.219
0.469GlnHis: 0.469 ± 0.083
2.496GlnIle: 2.496 ± 0.248
2.196GlnLys: 2.196 ± 0.294
3.19GlnLeu: 3.19 ± 0.244
0.807GlnMet: 0.807 ± 0.142
1.614GlnAsn: 1.614 ± 0.214
1.276GlnPro: 1.276 ± 0.156
1.426GlnGln: 1.426 ± 0.16
1.558GlnArg: 1.558 ± 0.179
2.702GlnSer: 2.702 ± 0.225
2.308GlnThr: 2.308 ± 0.239
2.852GlnVal: 2.852 ± 0.228
0.507GlnTrp: 0.507 ± 0.097
2.046GlnTyr: 2.046 ± 0.21
0.0GlnXaa: 0.0 ± 0.0
Arg
2.477ArgAla: 2.477 ± 0.212
0.319ArgCys: 0.319 ± 0.074
1.989ArgAsp: 1.989 ± 0.17
2.139ArgGlu: 2.139 ± 0.253
1.745ArgPhe: 1.745 ± 0.18
2.759ArgGly: 2.759 ± 0.24
0.732ArgHis: 0.732 ± 0.146
3.003ArgIle: 3.003 ± 0.241
2.308ArgLys: 2.308 ± 0.302
3.228ArgLeu: 3.228 ± 0.267
0.901ArgMet: 0.901 ± 0.143
2.102ArgAsn: 2.102 ± 0.188
1.445ArgPro: 1.445 ± 0.17
1.558ArgGln: 1.558 ± 0.139
1.67ArgArg: 1.67 ± 0.261
2.365ArgSer: 2.365 ± 0.243
2.496ArgThr: 2.496 ± 0.31
2.759ArgVal: 2.759 ± 0.239
0.45ArgTrp: 0.45 ± 0.104
2.158ArgTyr: 2.158 ± 0.201
0.0ArgXaa: 0.0 ± 0.0
Ser
6.005SerAla: 6.005 ± 0.375
0.507SerCys: 0.507 ± 0.106
4.654SerAsp: 4.654 ± 0.25
3.734SerGlu: 3.734 ± 0.333
3.678SerPhe: 3.678 ± 0.313
7.338SerGly: 7.338 ± 0.652
0.901SerHis: 0.901 ± 0.114
5.179SerIle: 5.179 ± 0.515
3.828SerLys: 3.828 ± 0.311
5.273SerLeu: 5.273 ± 0.241
1.445SerMet: 1.445 ± 0.206
4.56SerAsn: 4.56 ± 0.324
3.078SerPro: 3.078 ± 0.336
2.665SerGln: 2.665 ± 0.238
2.44SerArg: 2.44 ± 0.212
5.968SerSer: 5.968 ± 0.426
6.512SerThr: 6.512 ± 0.484
5.255SerVal: 5.255 ± 0.51
0.751SerTrp: 0.751 ± 0.125
3.003SerTyr: 3.003 ± 0.246
0.0SerXaa: 0.0 ± 0.0
Thr
6.549ThrAla: 6.549 ± 0.596
0.432ThrCys: 0.432 ± 0.088
4.448ThrAsp: 4.448 ± 0.306
4.147ThrGlu: 4.147 ± 0.335
3.359ThrPhe: 3.359 ± 0.375
7.187ThrGly: 7.187 ± 0.652
0.882ThrHis: 0.882 ± 0.142
5.179ThrIle: 5.179 ± 0.366
3.566ThrLys: 3.566 ± 0.252
6.381ThrLeu: 6.381 ± 0.411
1.088ThrMet: 1.088 ± 0.15
4.523ThrAsn: 4.523 ± 0.486
3.415ThrPro: 3.415 ± 0.276
2.515ThrGln: 2.515 ± 0.191
2.533ThrArg: 2.533 ± 0.279
6.212ThrSer: 6.212 ± 0.56
6.268ThrThr: 6.268 ± 0.625
5.949ThrVal: 5.949 ± 0.553
0.882ThrTrp: 0.882 ± 0.129
2.571ThrTyr: 2.571 ± 0.198
0.0ThrXaa: 0.0 ± 0.0
Val
4.41ValAla: 4.41 ± 0.376
0.338ValCys: 0.338 ± 0.076
5.33ValAsp: 5.33 ± 0.369
4.054ValGlu: 4.054 ± 0.277
2.552ValPhe: 2.552 ± 0.203
5.086ValGly: 5.086 ± 0.392
0.582ValHis: 0.582 ± 0.112
4.222ValIle: 4.222 ± 0.355
3.303ValLys: 3.303 ± 0.246
4.147ValLeu: 4.147 ± 0.272
1.314ValMet: 1.314 ± 0.161
4.429ValAsn: 4.429 ± 0.289
3.134ValPro: 3.134 ± 0.234
2.477ValGln: 2.477 ± 0.204
2.496ValArg: 2.496 ± 0.234
5.273ValSer: 5.273 ± 0.371
6.062ValThr: 6.062 ± 0.46
5.161ValVal: 5.161 ± 0.372
0.676ValTrp: 0.676 ± 0.106
2.421ValTyr: 2.421 ± 0.265
0.0ValXaa: 0.0 ± 0.0
Trp
0.863TrpAla: 0.863 ± 0.114
0.15TrpCys: 0.15 ± 0.052
0.751TrpAsp: 0.751 ± 0.137
0.525TrpGlu: 0.525 ± 0.136
0.413TrpPhe: 0.413 ± 0.1
0.638TrpGly: 0.638 ± 0.101
0.3TrpHis: 0.3 ± 0.086
0.713TrpIle: 0.713 ± 0.125
0.92TrpLys: 0.92 ± 0.159
0.676TrpLeu: 0.676 ± 0.114
0.394TrpMet: 0.394 ± 0.094
0.863TrpAsn: 0.863 ± 0.147
0.188TrpPro: 0.188 ± 0.064
0.394TrpGln: 0.394 ± 0.07
0.432TrpArg: 0.432 ± 0.108
0.938TrpSer: 0.938 ± 0.144
0.938TrpThr: 0.938 ± 0.133
0.732TrpVal: 0.732 ± 0.109
0.169TrpTrp: 0.169 ± 0.055
0.45TrpTyr: 0.45 ± 0.074
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.365TyrAla: 2.365 ± 0.178
0.45TyrCys: 0.45 ± 0.077
3.678TyrAsp: 3.678 ± 0.302
2.365TyrGlu: 2.365 ± 0.279
1.651TyrPhe: 1.651 ± 0.156
2.327TyrGly: 2.327 ± 0.209
0.544TyrHis: 0.544 ± 0.086
2.646TyrIle: 2.646 ± 0.226
2.477TyrLys: 2.477 ± 0.244
2.946TyrLeu: 2.946 ± 0.291
0.751TyrMet: 0.751 ± 0.145
2.721TyrAsn: 2.721 ± 0.252
1.483TyrPro: 1.483 ± 0.171
1.52TyrGln: 1.52 ± 0.152
2.139TyrArg: 2.139 ± 0.199
2.946TyrSer: 2.946 ± 0.274
2.965TyrThr: 2.965 ± 0.335
2.928TyrVal: 2.928 ± 0.234
0.357TyrTrp: 0.357 ± 0.097
1.952TyrTyr: 1.952 ± 0.19
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 210 proteins (53288 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski