Amino acid dipepetide frequency for Sphingobacterium sp. (strain 21)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.854AlaAla: 5.854 ± 0.076	0.721AlaCys: 0.721 ± 0.022	4.041AlaAsp: 4.041 ± 0.05	4.305AlaGlu: 4.305 ± 0.056	3.63AlaPhe: 3.63 ± 0.048	4.995AlaGly: 4.995 ± 0.066	1.329AlaHis: 1.329 ± 0.027	5.707AlaIle: 5.707 ± 0.073	4.656AlaLys: 4.656 ± 0.062	7.342AlaLeu: 7.342 ± 0.085	1.685AlaMet: 1.685 ± 0.03	3.751AlaAsn: 3.751 ± 0.054	2.295AlaPro: 2.295 ± 0.044	2.836AlaGln: 2.836 ± 0.047	2.893AlaArg: 2.893 ± 0.039	4.781AlaSer: 4.781 ± 0.055	3.806AlaThr: 3.806 ± 0.068	4.706AlaVal: 4.706 ± 0.06	0.942AlaTrp: 0.942 ± 0.023	3.155AlaTyr: 3.155 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	0.503CysAla: 0.503 ± 0.015	0.135CysCys: 0.135 ± 0.009	0.335CysAsp: 0.335 ± 0.013	0.357CysGlu: 0.357 ± 0.016	0.433CysPhe: 0.433 ± 0.015	0.567CysGly: 0.567 ± 0.02	0.192CysHis: 0.192 ± 0.013	0.614CysIle: 0.614 ± 0.017	0.409CysLys: 0.409 ± 0.016	0.866CysLeu: 0.866 ± 0.021	0.173CysMet: 0.173 ± 0.01	0.348CysAsn: 0.348 ± 0.013	0.31CysPro: 0.31 ± 0.012	0.235CysGln: 0.235 ± 0.01	0.326CysArg: 0.326 ± 0.015	0.525CysSer: 0.525 ± 0.017	0.42CysThr: 0.42 ± 0.016	0.441CysVal: 0.441 ± 0.014	0.088CysTrp: 0.088 ± 0.008	0.342CysTyr: 0.342 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	3.785AspAla: 3.785 ± 0.05	0.359AspCys: 0.359 ± 0.015	2.429AspAsp: 2.429 ± 0.039	3.457AspGlu: 3.457 ± 0.049	3.069AspPhe: 3.069 ± 0.038	3.806AspGly: 3.806 ± 0.058	1.071AspHis: 1.071 ± 0.025	3.971AspIle: 3.971 ± 0.05	3.479AspLys: 3.479 ± 0.051	5.19AspLeu: 5.19 ± 0.055	1.129AspMet: 1.129 ± 0.025	2.597AspAsn: 2.597 ± 0.04	2.153AspPro: 2.153 ± 0.036	1.862AspGln: 1.862 ± 0.034	2.602AspArg: 2.602 ± 0.041	2.831AspSer: 2.831 ± 0.046	2.427AspThr: 2.427 ± 0.04	3.253AspVal: 3.253 ± 0.047	0.853AspTrp: 0.853 ± 0.024	2.558AspTyr: 2.558 ± 0.034	0.0AspXaa: 0.0 ± 0.0
Glu	4.708GluAla: 4.708 ± 0.059	0.307GluCys: 0.307 ± 0.016	2.846GluAsp: 2.846 ± 0.041	4.445GluGlu: 4.445 ± 0.07	2.17GluPhe: 2.17 ± 0.035	3.714GluGly: 3.714 ± 0.044	1.268GluHis: 1.268 ± 0.026	4.468GluIle: 4.468 ± 0.057	4.763GluLys: 4.763 ± 0.065	5.876GluLeu: 5.876 ± 0.069	1.45GluMet: 1.45 ± 0.031	3.358GluAsn: 3.358 ± 0.048	1.726GluPro: 1.726 ± 0.033	2.902GluGln: 2.902 ± 0.044	3.212GluArg: 3.212 ± 0.047	3.072GluSer: 3.072 ± 0.039	2.998GluThr: 2.998 ± 0.047	3.91GluVal: 3.91 ± 0.049	0.726GluTrp: 0.726 ± 0.018	1.921GluTyr: 1.921 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	3.24PheAla: 3.24 ± 0.048	0.438PheCys: 0.438 ± 0.016	2.912PheAsp: 2.912 ± 0.046	2.716PheGlu: 2.716 ± 0.044	2.707PhePhe: 2.707 ± 0.046	3.393PheGly: 3.393 ± 0.043	0.921PheHis: 0.921 ± 0.024	3.516PheIle: 3.516 ± 0.05	2.935PheLys: 2.935 ± 0.047	4.636PheLeu: 4.636 ± 0.061	1.132PheMet: 1.132 ± 0.026	2.867PheAsn: 2.867 ± 0.044	1.769PhePro: 1.769 ± 0.029	1.531PheGln: 1.531 ± 0.025	2.045PheArg: 2.045 ± 0.034	3.735PheSer: 3.735 ± 0.054	2.817PheThr: 2.817 ± 0.035	2.885PheVal: 2.885 ± 0.044	0.638PheTrp: 0.638 ± 0.019	2.128PheTyr: 2.128 ± 0.038	0.0PheXaa: 0.0 ± 0.0
Gly	4.697GlyAla: 4.697 ± 0.055	0.552GlyCys: 0.552 ± 0.018	3.303GlyAsp: 3.303 ± 0.045	3.547GlyGlu: 3.547 ± 0.043	3.416GlyPhe: 3.416 ± 0.044	4.813GlyGly: 4.813 ± 0.067	1.248GlyHis: 1.248 ± 0.027	5.224GlyIle: 5.224 ± 0.061	5.11GlyLys: 5.11 ± 0.054	6.494GlyLeu: 6.494 ± 0.067	1.654GlyMet: 1.654 ± 0.028	3.67GlyAsn: 3.67 ± 0.063	1.63GlyPro: 1.63 ± 0.03	2.409GlyGln: 2.409 ± 0.044	2.907GlyArg: 2.907 ± 0.043	4.389GlySer: 4.389 ± 0.065	3.941GlyThr: 3.941 ± 0.057	4.488GlyVal: 4.488 ± 0.052	0.997GlyTrp: 0.997 ± 0.029	3.221GlyTyr: 3.221 ± 0.055	0.0GlyXaa: 0.0 ± 0.0
His	1.34HisAla: 1.34 ± 0.026	0.21HisCys: 0.21 ± 0.013	0.93HisAsp: 0.93 ± 0.023	1.049HisGlu: 1.049 ± 0.024	1.177HisPhe: 1.177 ± 0.028	1.207HisGly: 1.207 ± 0.028	0.585HisHis: 0.585 ± 0.019	1.549HisIle: 1.549 ± 0.031	1.044HisLys: 1.044 ± 0.023	2.151HisLeu: 2.151 ± 0.039	0.395HisMet: 0.395 ± 0.015	0.907HisAsn: 0.907 ± 0.023	1.017HisPro: 1.017 ± 0.028	0.849HisGln: 0.849 ± 0.024	0.96HisArg: 0.96 ± 0.027	1.068HisSer: 1.068 ± 0.024	1.048HisThr: 1.048 ± 0.022	1.129HisVal: 1.129 ± 0.026	0.299HisTrp: 0.299 ± 0.013	0.984HisTyr: 0.984 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	6.019IleAla: 6.019 ± 0.073	0.653IleCys: 0.653 ± 0.019	4.338IleAsp: 4.338 ± 0.05	4.376IleGlu: 4.376 ± 0.056	3.093IlePhe: 3.093 ± 0.051	5.082IleGly: 5.082 ± 0.058	1.431IleHis: 1.431 ± 0.03	4.826IleIle: 4.826 ± 0.062	4.686IleLys: 4.686 ± 0.057	6.359IleLeu: 6.359 ± 0.075	1.348IleMet: 1.348 ± 0.031	4.082IleAsn: 4.082 ± 0.05	3.244IlePro: 3.244 ± 0.044	2.548IleGln: 2.548 ± 0.039	3.343IleArg: 3.343 ± 0.043	4.918IleSer: 4.918 ± 0.057	4.076IleThr: 4.076 ± 0.047	4.222IleVal: 4.222 ± 0.054	0.773IleTrp: 0.773 ± 0.024	2.718IleTyr: 2.718 ± 0.042	0.0IleXaa: 0.0 ± 0.0
Lys	4.902LysAla: 4.902 ± 0.06	0.262LysCys: 0.262 ± 0.012	3.693LysAsp: 3.693 ± 0.055	5.178LysGlu: 5.178 ± 0.066	2.201LysPhe: 2.201 ± 0.038	4.418LysGly: 4.418 ± 0.046	1.387LysHis: 1.387 ± 0.029	4.561LysIle: 4.561 ± 0.061	5.053LysLys: 5.053 ± 0.062	5.897LysLeu: 5.897 ± 0.056	1.591LysMet: 1.591 ± 0.031	3.801LysAsn: 3.801 ± 0.048	2.558LysPro: 2.558 ± 0.043	2.94LysGln: 2.94 ± 0.04	3.246LysArg: 3.246 ± 0.048	3.788LysSer: 3.788 ± 0.05	3.649LysThr: 3.649 ± 0.05	3.995LysVal: 3.995 ± 0.049	0.866LysTrp: 0.866 ± 0.023	2.439LysTyr: 2.439 ± 0.04	0.0LysXaa: 0.0 ± 0.0
Leu	7.445LeuAla: 7.445 ± 0.064	0.829LeuCys: 0.829 ± 0.024	4.931LeuAsp: 4.931 ± 0.055	5.205LeuGlu: 5.205 ± 0.061	5.089LeuPhe: 5.089 ± 0.058	6.156LeuGly: 6.156 ± 0.073	1.955LeuHis: 1.955 ± 0.033	6.841LeuIle: 6.841 ± 0.079	6.83LeuLys: 6.83 ± 0.064	10.447LeuLeu: 10.447 ± 0.105	2.249LeuMet: 2.249 ± 0.034	5.378LeuAsn: 5.378 ± 0.066	4.181LeuPro: 4.181 ± 0.056	3.88LeuGln: 3.88 ± 0.049	4.467LeuArg: 4.467 ± 0.055	7.358LeuSer: 7.358 ± 0.078	5.484LeuThr: 5.484 ± 0.05	5.621LeuVal: 5.621 ± 0.064	1.041LeuTrp: 1.041 ± 0.024	3.614LeuTyr: 3.614 ± 0.045	0.0LeuXaa: 0.0 ± 0.0
Met	1.764MetAla: 1.764 ± 0.032	0.131MetCys: 0.131 ± 0.009	1.203MetAsp: 1.203 ± 0.024	1.489MetGlu: 1.489 ± 0.029	0.764MetPhe: 0.764 ± 0.023	1.542MetGly: 1.542 ± 0.031	0.459MetHis: 0.459 ± 0.018	1.441MetIle: 1.441 ± 0.033	1.828MetLys: 1.828 ± 0.032	2.228MetLeu: 2.228 ± 0.033	0.557MetMet: 0.557 ± 0.017	1.251MetAsn: 1.251 ± 0.027	1.017MetPro: 1.017 ± 0.025	0.928MetGln: 0.928 ± 0.019	1.082MetArg: 1.082 ± 0.029	1.227MetSer: 1.227 ± 0.024	1.095MetThr: 1.095 ± 0.022	1.357MetVal: 1.357 ± 0.029	0.198MetTrp: 0.198 ± 0.01	0.66MetTyr: 0.66 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	3.763AsnAla: 3.763 ± 0.054	0.349AsnCys: 0.349 ± 0.015	2.686AsnAsp: 2.686 ± 0.042	3.157AsnGlu: 3.157 ± 0.042	2.574AsnPhe: 2.574 ± 0.045	3.897AsnGly: 3.897 ± 0.062	1.072AsnHis: 1.072 ± 0.024	3.858AsnIle: 3.858 ± 0.056	3.481AsnLys: 3.481 ± 0.052	5.006AsnLeu: 5.006 ± 0.059	1.204AsnMet: 1.204 ± 0.026	3.023AsnAsn: 3.023 ± 0.052	2.646AsnPro: 2.646 ± 0.045	2.189AsnGln: 2.189 ± 0.038	2.773AsnArg: 2.773 ± 0.04	2.994AsnSer: 2.994 ± 0.049	3.057AsnThr: 3.057 ± 0.046	3.045AsnVal: 3.045 ± 0.05	0.762AsnTrp: 0.762 ± 0.021	2.537AsnTyr: 2.537 ± 0.048	0.0AsnXaa: 0.0 ± 0.0
Pro	2.701ProAla: 2.701 ± 0.042	0.225ProCys: 0.225 ± 0.011	2.364ProAsp: 2.364 ± 0.035	2.674ProGlu: 2.674 ± 0.041	1.984ProPhe: 1.984 ± 0.028	2.537ProGly: 2.537 ± 0.041	0.731ProHis: 0.731 ± 0.019	2.835ProIle: 2.835 ± 0.036	2.216ProLys: 2.216 ± 0.036	3.694ProLeu: 3.694 ± 0.042	0.774ProMet: 0.774 ± 0.023	2.1ProAsn: 2.1 ± 0.035	1.027ProPro: 1.027 ± 0.026	1.421ProGln: 1.421 ± 0.027	1.259ProArg: 1.259 ± 0.026	2.479ProSer: 2.479 ± 0.043	2.1ProThr: 2.1 ± 0.037	2.643ProVal: 2.643 ± 0.044	0.487ProTrp: 0.487 ± 0.017	1.684ProTyr: 1.684 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	2.997GlnAla: 2.997 ± 0.046	0.211GlnCys: 0.211 ± 0.012	1.811GlnAsp: 1.811 ± 0.027	2.547GlnGlu: 2.547 ± 0.042	1.708GlnPhe: 1.708 ± 0.034	2.354GlnGly: 2.354 ± 0.039	0.939GlnHis: 0.939 ± 0.023	2.579GlnIle: 2.579 ± 0.038	2.382GlnLys: 2.382 ± 0.037	4.311GlnLeu: 4.311 ± 0.053	0.872GlnMet: 0.872 ± 0.022	1.893GlnAsn: 1.893 ± 0.032	1.375GlnPro: 1.375 ± 0.029	2.223GlnGln: 2.223 ± 0.042	1.928GlnArg: 1.928 ± 0.038	2.229GlnSer: 2.229 ± 0.036	2.026GlnThr: 2.026 ± 0.034	2.481GlnVal: 2.481 ± 0.038	0.532GlnTrp: 0.532 ± 0.019	1.623GlnTyr: 1.623 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	2.956ArgAla: 2.956 ± 0.045	0.27ArgCys: 0.27 ± 0.012	2.25ArgAsp: 2.25 ± 0.037	2.673ArgGlu: 2.673 ± 0.041	2.49ArgPhe: 2.49 ± 0.039	2.471ArgGly: 2.471 ± 0.039	0.798ArgHis: 0.798 ± 0.02	3.544ArgIle: 3.544 ± 0.047	3.201ArgLys: 3.201 ± 0.045	4.641ArgLeu: 4.641 ± 0.05	1.134ArgMet: 1.134 ± 0.026	2.584ArgAsn: 2.584 ± 0.039	1.582ArgPro: 1.582 ± 0.031	1.728ArgGln: 1.728 ± 0.03	1.999ArgArg: 1.999 ± 0.035	2.733ArgSer: 2.733 ± 0.049	2.302ArgThr: 2.302 ± 0.032	2.731ArgVal: 2.731 ± 0.035	0.688ArgTrp: 0.688 ± 0.022	2.219ArgTyr: 2.219 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	4.366SerAla: 4.366 ± 0.048	0.572SerCys: 0.572 ± 0.019	3.154SerAsp: 3.154 ± 0.044	3.248SerGlu: 3.248 ± 0.043	3.763SerPhe: 3.763 ± 0.054	4.569SerGly: 4.569 ± 0.06	1.135SerHis: 1.135 ± 0.026	4.836SerIle: 4.836 ± 0.047	3.736SerLys: 3.736 ± 0.048	6.801SerLeu: 6.801 ± 0.071	1.37SerMet: 1.37 ± 0.027	3.115SerAsn: 3.115 ± 0.047	2.363SerPro: 2.363 ± 0.033	2.089SerGln: 2.089 ± 0.036	2.569SerArg: 2.569 ± 0.04	4.274SerSer: 4.274 ± 0.052	3.511SerThr: 3.511 ± 0.054	4.024SerVal: 4.024 ± 0.045	0.829SerTrp: 0.829 ± 0.022	2.98SerTyr: 2.98 ± 0.048	0.0SerXaa: 0.0 ± 0.0
Thr	4.216ThrAla: 4.216 ± 0.049	0.363ThrCys: 0.363 ± 0.015	3.167ThrAsp: 3.167 ± 0.04	2.942ThrGlu: 2.942 ± 0.04	2.705ThrPhe: 2.705 ± 0.041	4.287ThrGly: 4.287 ± 0.057	1.0ThrHis: 1.0 ± 0.024	3.976ThrIle: 3.976 ± 0.046	3.156ThrLys: 3.156 ± 0.042	5.427ThrLeu: 5.427 ± 0.058	1.011ThrMet: 1.011 ± 0.025	2.755ThrAsn: 2.755 ± 0.047	2.386ThrPro: 2.386 ± 0.039	1.81ThrGln: 1.81 ± 0.035	1.941ThrArg: 1.941 ± 0.037	3.318ThrSer: 3.318 ± 0.048	3.052ThrThr: 3.052 ± 0.057	3.593ThrVal: 3.593 ± 0.058	0.68ThrTrp: 0.68 ± 0.021	2.287ThrTyr: 2.287 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	4.413ValAla: 4.413 ± 0.055	0.536ValCys: 0.536 ± 0.019	3.501ValAsp: 3.501 ± 0.051	3.451ValGlu: 3.451 ± 0.052	3.125ValPhe: 3.125 ± 0.045	4.016ValGly: 4.016 ± 0.051	1.113ValHis: 1.113 ± 0.029	4.337ValIle: 4.337 ± 0.052	4.031ValLys: 4.031 ± 0.052	6.163ValLeu: 6.163 ± 0.066	1.311ValMet: 1.311 ± 0.029	3.49ValAsn: 3.49 ± 0.047	2.46ValPro: 2.46 ± 0.036	2.127ValGln: 2.127 ± 0.037	2.59ValArg: 2.59 ± 0.042	4.395ValSer: 4.395 ± 0.06	3.248ValThr: 3.248 ± 0.062	4.048ValVal: 4.048 ± 0.061	0.719ValTrp: 0.719 ± 0.021	2.481ValTyr: 2.481 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	0.792TrpAla: 0.792 ± 0.022	0.123TrpCys: 0.123 ± 0.009	0.682TrpAsp: 0.682 ± 0.021	0.798TrpGlu: 0.798 ± 0.019	0.6TrpPhe: 0.6 ± 0.02	0.912TrpGly: 0.912 ± 0.023	0.292TrpHis: 0.292 ± 0.013	0.821TrpIle: 0.821 ± 0.025	0.934TrpLys: 0.934 ± 0.025	1.369TrpLeu: 1.369 ± 0.031	0.359TrpMet: 0.359 ± 0.014	0.708TrpAsn: 0.708 ± 0.022	0.425TrpPro: 0.425 ± 0.017	0.628TrpGln: 0.628 ± 0.02	0.612TrpArg: 0.612 ± 0.02	0.735TrpSer: 0.735 ± 0.02	0.664TrpThr: 0.664 ± 0.019	0.73TrpVal: 0.73 ± 0.02	0.227TrpTrp: 0.227 ± 0.012	0.516TrpTyr: 0.516 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.967TyrAla: 2.967 ± 0.046	0.375TyrCys: 0.375 ± 0.015	2.302TyrAsp: 2.302 ± 0.038	2.223TyrGlu: 2.223 ± 0.036	2.264TyrPhe: 2.264 ± 0.042	2.973TyrGly: 2.973 ± 0.047	0.971TyrHis: 0.971 ± 0.028	2.63TyrIle: 2.63 ± 0.041	2.496TyrLys: 2.496 ± 0.036	4.106TyrLeu: 4.106 ± 0.052	0.824TyrMet: 0.824 ± 0.025	2.395TyrAsn: 2.395 ± 0.038	1.772TyrPro: 1.772 ± 0.032	1.889TyrGln: 1.889 ± 0.033	2.239TyrArg: 2.239 ± 0.038	2.455TyrSer: 2.455 ± 0.041	2.35TyrThr: 2.35 ± 0.039	2.252TyrVal: 2.252 ± 0.038	0.583TyrTrp: 0.583 ± 0.019	1.931TyrTyr: 1.931 ± 0.038	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5132 proteins (1809840 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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