Amino acid dipepetide frequency for Mycobacterium phage Malithi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.627AlaAla: 17.627 ± 1.584
1.045AlaCys: 1.045 ± 0.261
8.291AlaAsp: 8.291 ± 0.969
10.576AlaGlu: 10.576 ± 1.397
3.003AlaPhe: 3.003 ± 0.418
9.14AlaGly: 9.14 ± 1.011
2.22AlaHis: 2.22 ± 0.394
5.745AlaIle: 5.745 ± 0.629
4.505AlaLys: 4.505 ± 0.66
10.119AlaLeu: 10.119 ± 0.789
2.481AlaMet: 2.481 ± 0.33
3.852AlaAsn: 3.852 ± 0.527
7.443AlaPro: 7.443 ± 0.997
4.635AlaGln: 4.635 ± 0.667
8.422AlaArg: 8.422 ± 0.843
6.529AlaSer: 6.529 ± 0.706
6.594AlaThr: 6.594 ± 0.778
6.072AlaVal: 6.072 ± 0.651
2.22AlaTrp: 2.22 ± 0.468
2.546AlaTyr: 2.546 ± 0.364
0.0AlaXaa: 0.0 ± 0.0
Cys
0.783CysAla: 0.783 ± 0.24
0.131CysCys: 0.131 ± 0.099
0.718CysAsp: 0.718 ± 0.241
0.783CysGlu: 0.783 ± 0.242
0.065CysPhe: 0.065 ± 0.054
1.371CysGly: 1.371 ± 0.341
0.326CysHis: 0.326 ± 0.153
0.392CysIle: 0.392 ± 0.181
0.196CysLys: 0.196 ± 0.111
0.522CysLeu: 0.522 ± 0.186
0.261CysMet: 0.261 ± 0.128
0.261CysAsn: 0.261 ± 0.118
1.11CysPro: 1.11 ± 0.379
0.457CysGln: 0.457 ± 0.194
0.849CysArg: 0.849 ± 0.197
0.588CysSer: 0.588 ± 0.216
0.849CysThr: 0.849 ± 0.233
0.261CysVal: 0.261 ± 0.132
0.131CysTrp: 0.131 ± 0.088
0.196CysTyr: 0.196 ± 0.12
0.0CysXaa: 0.0 ± 0.0
Asp
8.096AspAla: 8.096 ± 0.977
1.045AspCys: 1.045 ± 0.331
6.333AspAsp: 6.333 ± 0.786
5.811AspGlu: 5.811 ± 0.706
1.306AspPhe: 1.306 ± 0.301
6.79AspGly: 6.79 ± 0.743
1.175AspHis: 1.175 ± 0.281
2.154AspIle: 2.154 ± 0.325
1.502AspLys: 1.502 ± 0.265
5.484AspLeu: 5.484 ± 0.63
1.24AspMet: 1.24 ± 0.271
1.306AspAsn: 1.306 ± 0.258
4.505AspPro: 4.505 ± 0.696
1.567AspGln: 1.567 ± 0.336
4.635AspArg: 4.635 ± 0.503
2.742AspSer: 2.742 ± 0.738
3.264AspThr: 3.264 ± 0.464
3.787AspVal: 3.787 ± 0.435
1.567AspTrp: 1.567 ± 0.307
1.567AspTyr: 1.567 ± 0.285
0.0AspXaa: 0.0 ± 0.0
Glu
7.639GluAla: 7.639 ± 0.838
0.914GluCys: 0.914 ± 0.281
2.807GluAsp: 2.807 ± 0.438
2.807GluGlu: 2.807 ± 0.504
1.893GluPhe: 1.893 ± 0.316
3.721GluGly: 3.721 ± 0.55
1.763GluHis: 1.763 ± 0.36
3.917GluIle: 3.917 ± 0.523
1.763GluLys: 1.763 ± 0.411
6.594GluLeu: 6.594 ± 0.764
1.567GluMet: 1.567 ± 0.371
1.371GluAsn: 1.371 ± 0.254
3.46GluPro: 3.46 ± 0.56
2.611GluGln: 2.611 ± 0.405
3.852GluArg: 3.852 ± 0.536
2.938GluSer: 2.938 ± 0.561
3.46GluThr: 3.46 ± 0.498
3.852GluVal: 3.852 ± 0.483
1.697GluTrp: 1.697 ± 0.334
1.436GluTyr: 1.436 ± 0.276
0.0GluXaa: 0.0 ± 0.0
Phe
2.285PheAla: 2.285 ± 0.432
0.131PheCys: 0.131 ± 0.09
2.35PheAsp: 2.35 ± 0.467
2.35PheGlu: 2.35 ± 0.386
0.457PhePhe: 0.457 ± 0.152
3.787PheGly: 3.787 ± 0.539
0.457PheHis: 0.457 ± 0.157
1.567PheIle: 1.567 ± 0.244
0.979PheLys: 0.979 ± 0.234
1.632PheLeu: 1.632 ± 0.342
0.849PheMet: 0.849 ± 0.253
1.175PheAsn: 1.175 ± 0.366
1.828PhePro: 1.828 ± 0.398
1.11PheGln: 1.11 ± 0.269
1.763PheArg: 1.763 ± 0.342
1.175PheSer: 1.175 ± 0.335
1.567PheThr: 1.567 ± 0.341
1.828PheVal: 1.828 ± 0.294
0.261PheTrp: 0.261 ± 0.125
0.522PheTyr: 0.522 ± 0.195
0.0PheXaa: 0.0 ± 0.0
Gly
9.989GlyAla: 9.989 ± 1.299
0.326GlyCys: 0.326 ± 0.15
4.635GlyAsp: 4.635 ± 0.58
4.57GlyGlu: 4.57 ± 0.682
3.525GlyPhe: 3.525 ± 0.62
9.336GlyGly: 9.336 ± 1.594
1.24GlyHis: 1.24 ± 0.286
3.525GlyIle: 3.525 ± 0.462
3.33GlyLys: 3.33 ± 0.414
7.573GlyLeu: 7.573 ± 0.931
1.24GlyMet: 1.24 ± 0.3
2.481GlyAsn: 2.481 ± 0.416
4.244GlyPro: 4.244 ± 0.736
3.983GlyGln: 3.983 ± 0.595
5.615GlyArg: 5.615 ± 0.668
5.811GlySer: 5.811 ± 0.865
5.419GlyThr: 5.419 ± 0.649
7.051GlyVal: 7.051 ± 0.782
1.567GlyTrp: 1.567 ± 0.347
2.416GlyTyr: 2.416 ± 0.383
0.0GlyXaa: 0.0 ± 0.0
His
1.763HisAla: 1.763 ± 0.346
0.131HisCys: 0.131 ± 0.101
1.697HisAsp: 1.697 ± 0.302
1.371HisGlu: 1.371 ± 0.4
0.326HisPhe: 0.326 ± 0.157
0.914HisGly: 0.914 ± 0.265
0.522HisHis: 0.522 ± 0.177
0.979HisIle: 0.979 ± 0.208
0.849HisLys: 0.849 ± 0.217
1.24HisLeu: 1.24 ± 0.254
0.457HisMet: 0.457 ± 0.164
0.718HisAsn: 0.718 ± 0.224
1.175HisPro: 1.175 ± 0.302
0.718HisGln: 0.718 ± 0.234
1.763HisArg: 1.763 ± 0.355
0.718HisSer: 0.718 ± 0.213
1.502HisThr: 1.502 ± 0.387
1.371HisVal: 1.371 ± 0.348
0.392HisTrp: 0.392 ± 0.182
0.653HisTyr: 0.653 ± 0.232
0.0HisXaa: 0.0 ± 0.0
Ile
6.137IleAla: 6.137 ± 0.546
0.392IleCys: 0.392 ± 0.156
3.068IleAsp: 3.068 ± 0.437
3.983IleGlu: 3.983 ± 0.569
0.457IlePhe: 0.457 ± 0.172
3.917IleGly: 3.917 ± 0.588
0.979IleHis: 0.979 ± 0.231
0.783IleIle: 0.783 ± 0.208
1.11IleLys: 1.11 ± 0.255
1.697IleLeu: 1.697 ± 0.297
0.457IleMet: 0.457 ± 0.147
1.045IleAsn: 1.045 ± 0.276
2.742IlePro: 2.742 ± 0.382
1.567IleGln: 1.567 ± 0.317
4.178IleArg: 4.178 ± 0.577
2.546IleSer: 2.546 ± 0.467
3.264IleThr: 3.264 ± 0.515
2.938IleVal: 2.938 ± 0.538
0.588IleTrp: 0.588 ± 0.177
0.914IleTyr: 0.914 ± 0.295
0.0IleXaa: 0.0 ± 0.0
Lys
4.048LysAla: 4.048 ± 0.533
0.326LysCys: 0.326 ± 0.167
1.306LysAsp: 1.306 ± 0.269
1.045LysGlu: 1.045 ± 0.241
1.11LysPhe: 1.11 ± 0.282
1.828LysGly: 1.828 ± 0.427
0.588LysHis: 0.588 ± 0.201
0.914LysIle: 0.914 ± 0.227
0.718LysLys: 0.718 ± 0.205
3.721LysLeu: 3.721 ± 0.425
0.914LysMet: 0.914 ± 0.262
0.653LysAsn: 0.653 ± 0.251
3.003LysPro: 3.003 ± 0.397
0.783LysGln: 0.783 ± 0.252
2.22LysArg: 2.22 ± 0.395
1.567LysSer: 1.567 ± 0.313
2.416LysThr: 2.416 ± 0.464
2.416LysVal: 2.416 ± 0.301
0.196LysTrp: 0.196 ± 0.112
0.718LysTyr: 0.718 ± 0.177
0.0LysXaa: 0.0 ± 0.0
Leu
10.642LeuAla: 10.642 ± 0.824
0.914LeuCys: 0.914 ± 0.26
6.659LeuAsp: 6.659 ± 0.754
3.395LeuGlu: 3.395 ± 0.451
1.502LeuPhe: 1.502 ± 0.28
7.508LeuGly: 7.508 ± 0.774
1.24LeuHis: 1.24 ± 0.284
4.048LeuIle: 4.048 ± 0.502
2.22LeuLys: 2.22 ± 0.369
6.529LeuLeu: 6.529 ± 0.729
1.24LeuMet: 1.24 ± 0.309
2.024LeuAsn: 2.024 ± 0.436
5.68LeuPro: 5.68 ± 0.603
2.416LeuGln: 2.416 ± 0.44
6.268LeuArg: 6.268 ± 0.518
4.44LeuSer: 4.44 ± 0.642
5.223LeuThr: 5.223 ± 0.581
4.766LeuVal: 4.766 ± 0.566
1.24LeuTrp: 1.24 ± 0.283
1.306LeuTyr: 1.306 ± 0.294
0.0LeuXaa: 0.0 ± 0.0
Met
3.264MetAla: 3.264 ± 0.439
0.196MetCys: 0.196 ± 0.105
0.849MetAsp: 0.849 ± 0.227
0.588MetGlu: 0.588 ± 0.216
0.653MetPhe: 0.653 ± 0.204
0.914MetGly: 0.914 ± 0.302
0.261MetHis: 0.261 ± 0.134
0.718MetIle: 0.718 ± 0.277
0.457MetLys: 0.457 ± 0.179
1.436MetLeu: 1.436 ± 0.32
0.588MetMet: 0.588 ± 0.183
0.653MetAsn: 0.653 ± 0.187
1.567MetPro: 1.567 ± 0.33
0.653MetGln: 0.653 ± 0.229
1.632MetArg: 1.632 ± 0.338
2.024MetSer: 2.024 ± 0.382
2.22MetThr: 2.22 ± 0.384
0.783MetVal: 0.783 ± 0.207
0.196MetTrp: 0.196 ± 0.131
0.392MetTyr: 0.392 ± 0.178
0.0MetXaa: 0.0 ± 0.0
Asn
3.656AsnAla: 3.656 ± 0.6
0.261AsnCys: 0.261 ± 0.131
1.436AsnAsp: 1.436 ± 0.358
1.24AsnGlu: 1.24 ± 0.302
0.522AsnPhe: 0.522 ± 0.246
3.395AsnGly: 3.395 ± 0.48
0.718AsnHis: 0.718 ± 0.261
0.979AsnIle: 0.979 ± 0.195
0.457AsnLys: 0.457 ± 0.178
2.024AsnLeu: 2.024 ± 0.354
0.522AsnMet: 0.522 ± 0.168
0.979AsnAsn: 0.979 ± 0.241
3.134AsnPro: 3.134 ± 0.417
0.457AsnGln: 0.457 ± 0.194
2.481AsnArg: 2.481 ± 0.393
2.35AsnSer: 2.35 ± 0.413
2.024AsnThr: 2.024 ± 0.379
1.893AsnVal: 1.893 ± 0.335
0.783AsnTrp: 0.783 ± 0.219
0.326AsnTyr: 0.326 ± 0.149
0.0AsnXaa: 0.0 ± 0.0
Pro
7.312ProAla: 7.312 ± 0.815
0.392ProCys: 0.392 ± 0.18
4.44ProAsp: 4.44 ± 0.564
3.525ProGlu: 3.525 ± 0.56
2.089ProPhe: 2.089 ± 0.347
7.051ProGly: 7.051 ± 0.9
0.653ProHis: 0.653 ± 0.231
2.22ProIle: 2.22 ± 0.348
2.22ProLys: 2.22 ± 0.367
4.831ProLeu: 4.831 ± 0.577
0.783ProMet: 0.783 ± 0.233
2.677ProAsn: 2.677 ± 0.655
3.134ProPro: 3.134 ± 0.497
2.546ProGln: 2.546 ± 0.386
4.766ProArg: 4.766 ± 0.625
3.134ProSer: 3.134 ± 0.447
4.244ProThr: 4.244 ± 0.449
4.635ProVal: 4.635 ± 0.472
1.371ProTrp: 1.371 ± 0.272
1.371ProTyr: 1.371 ± 0.269
0.0ProXaa: 0.0 ± 0.0
Gln
3.983GlnAla: 3.983 ± 0.636
0.261GlnCys: 0.261 ± 0.131
1.306GlnAsp: 1.306 ± 0.338
1.567GlnGlu: 1.567 ± 0.312
1.763GlnPhe: 1.763 ± 0.445
3.003GlnGly: 3.003 ± 0.632
0.653GlnHis: 0.653 ± 0.184
1.893GlnIle: 1.893 ± 0.378
1.436GlnLys: 1.436 ± 0.296
2.481GlnLeu: 2.481 ± 0.433
1.045GlnMet: 1.045 ± 0.259
0.849GlnAsn: 0.849 ± 0.265
2.481GlnPro: 2.481 ± 0.388
2.807GlnGln: 2.807 ± 0.441
2.873GlnArg: 2.873 ± 0.534
1.959GlnSer: 1.959 ± 0.384
2.024GlnThr: 2.024 ± 0.335
3.199GlnVal: 3.199 ± 0.497
1.045GlnTrp: 1.045 ± 0.242
0.718GlnTyr: 0.718 ± 0.199
0.0GlnXaa: 0.0 ± 0.0
Arg
8.814ArgAla: 8.814 ± 1.025
0.914ArgCys: 0.914 ± 0.258
5.288ArgAsp: 5.288 ± 0.637
4.178ArgGlu: 4.178 ± 0.738
2.22ArgPhe: 2.22 ± 0.376
4.57ArgGly: 4.57 ± 0.567
1.828ArgHis: 1.828 ± 0.376
3.003ArgIle: 3.003 ± 0.404
2.938ArgLys: 2.938 ± 0.426
5.158ArgLeu: 5.158 ± 0.544
2.089ArgMet: 2.089 ± 0.316
2.285ArgAsn: 2.285 ± 0.41
3.656ArgPro: 3.656 ± 0.52
2.611ArgGln: 2.611 ± 0.431
6.986ArgArg: 6.986 ± 1.147
3.656ArgSer: 3.656 ± 0.65
4.505ArgThr: 4.505 ± 0.52
5.223ArgVal: 5.223 ± 0.728
1.502ArgTrp: 1.502 ± 0.361
1.763ArgTyr: 1.763 ± 0.366
0.0ArgXaa: 0.0 ± 0.0
Ser
7.182SerAla: 7.182 ± 0.784
0.588SerCys: 0.588 ± 0.213
3.264SerAsp: 3.264 ± 0.513
2.416SerGlu: 2.416 ± 0.476
1.436SerPhe: 1.436 ± 0.354
6.529SerGly: 6.529 ± 1.157
1.045SerHis: 1.045 ± 0.257
2.024SerIle: 2.024 ± 0.436
1.567SerLys: 1.567 ± 0.374
4.113SerLeu: 4.113 ± 0.518
1.371SerMet: 1.371 ± 0.277
1.567SerAsn: 1.567 ± 0.405
2.873SerPro: 2.873 ± 0.456
1.697SerGln: 1.697 ± 0.342
3.917SerArg: 3.917 ± 0.65
2.416SerSer: 2.416 ± 0.516
3.983SerThr: 3.983 ± 0.601
4.244SerVal: 4.244 ± 0.518
0.783SerTrp: 0.783 ± 0.22
1.045SerTyr: 1.045 ± 0.239
0.0SerXaa: 0.0 ± 0.0
Thr
7.051ThrAla: 7.051 ± 0.656
0.392ThrCys: 0.392 ± 0.152
4.113ThrAsp: 4.113 ± 0.579
3.199ThrGlu: 3.199 ± 0.511
1.567ThrPhe: 1.567 ± 0.452
5.484ThrGly: 5.484 ± 0.594
1.175ThrHis: 1.175 ± 0.274
4.113ThrIle: 4.113 ± 0.648
1.828ThrLys: 1.828 ± 0.414
5.288ThrLeu: 5.288 ± 0.513
1.11ThrMet: 1.11 ± 0.332
1.828ThrAsn: 1.828 ± 0.29
5.158ThrPro: 5.158 ± 0.511
2.481ThrGln: 2.481 ± 0.363
3.721ThrArg: 3.721 ± 0.573
2.873ThrSer: 2.873 ± 0.381
4.113ThrThr: 4.113 ± 0.495
6.333ThrVal: 6.333 ± 0.745
1.24ThrTrp: 1.24 ± 0.276
1.697ThrTyr: 1.697 ± 0.376
0.0ThrXaa: 0.0 ± 0.0
Val
8.096ValAla: 8.096 ± 0.797
0.979ValCys: 0.979 ± 0.301
5.484ValAsp: 5.484 ± 0.656
4.635ValGlu: 4.635 ± 0.535
2.22ValPhe: 2.22 ± 0.396
5.549ValGly: 5.549 ± 0.571
1.24ValHis: 1.24 ± 0.234
2.285ValIle: 2.285 ± 0.385
1.567ValLys: 1.567 ± 0.251
5.027ValLeu: 5.027 ± 0.613
1.24ValMet: 1.24 ± 0.26
2.742ValAsn: 2.742 ± 0.33
3.983ValPro: 3.983 ± 0.563
2.154ValGln: 2.154 ± 0.343
4.048ValArg: 4.048 ± 0.593
4.048ValSer: 4.048 ± 0.56
5.288ValThr: 5.288 ± 0.707
6.659ValVal: 6.659 ± 0.957
1.175ValTrp: 1.175 ± 0.254
1.436ValTyr: 1.436 ± 0.315
0.0ValXaa: 0.0 ± 0.0
Trp
2.089TrpAla: 2.089 ± 0.43
0.588TrpCys: 0.588 ± 0.205
0.718TrpAsp: 0.718 ± 0.196
0.653TrpGlu: 0.653 ± 0.177
1.175TrpPhe: 1.175 ± 0.299
1.11TrpGly: 1.11 ± 0.248
0.392TrpHis: 0.392 ± 0.168
0.849TrpIle: 0.849 ± 0.281
0.326TrpLys: 0.326 ± 0.131
1.632TrpLeu: 1.632 ± 0.297
0.261TrpMet: 0.261 ± 0.136
0.588TrpAsn: 0.588 ± 0.221
1.045TrpPro: 1.045 ± 0.308
1.24TrpGln: 1.24 ± 0.248
1.632TrpArg: 1.632 ± 0.339
1.306TrpSer: 1.306 ± 0.26
1.175TrpThr: 1.175 ± 0.301
1.045TrpVal: 1.045 ± 0.242
0.522TrpTrp: 0.522 ± 0.173
0.457TrpTyr: 0.457 ± 0.179
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.611TyrAla: 2.611 ± 0.415
0.326TyrCys: 0.326 ± 0.135
1.11TyrAsp: 1.11 ± 0.289
1.371TyrGlu: 1.371 ± 0.236
0.783TyrPhe: 0.783 ± 0.202
1.959TyrGly: 1.959 ± 0.384
0.783TyrHis: 0.783 ± 0.253
0.653TyrIle: 0.653 ± 0.27
0.522TyrLys: 0.522 ± 0.217
2.154TyrLeu: 2.154 ± 0.39
0.261TyrMet: 0.261 ± 0.122
0.588TyrAsn: 0.588 ± 0.171
1.175TyrPro: 1.175 ± 0.326
0.849TyrGln: 0.849 ± 0.277
1.567TyrArg: 1.567 ± 0.3
1.24TyrSer: 1.24 ± 0.256
1.632TyrThr: 1.632 ± 0.364
1.502TyrVal: 1.502 ± 0.321
0.392TyrTrp: 0.392 ± 0.143
0.457TyrTyr: 0.457 ± 0.168
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 79 proteins (15318 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski