Amino acid dipepetide frequency for Bacillus sp. HMF5848

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.405AlaAla: 5.405 ± 0.101
0.635AlaCys: 0.635 ± 0.028
3.408AlaAsp: 3.408 ± 0.053
4.296AlaGlu: 4.296 ± 0.072
3.239AlaPhe: 3.239 ± 0.056
4.909AlaGly: 4.909 ± 0.078
1.351AlaHis: 1.351 ± 0.041
6.126AlaIle: 6.126 ± 0.08
4.567AlaLys: 4.567 ± 0.07
7.13AlaLeu: 7.13 ± 0.088
2.018AlaMet: 2.018 ± 0.045
3.085AlaAsn: 3.085 ± 0.054
2.093AlaPro: 2.093 ± 0.052
2.226AlaGln: 2.226 ± 0.055
2.57AlaArg: 2.57 ± 0.047
4.108AlaSer: 4.108 ± 0.063
3.916AlaThr: 3.916 ± 0.065
5.358AlaVal: 5.358 ± 0.069
0.632AlaTrp: 0.632 ± 0.026
2.425AlaTyr: 2.425 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
0.433CysAla: 0.433 ± 0.018
0.096CysCys: 0.096 ± 0.011
0.419CysAsp: 0.419 ± 0.02
0.513CysGlu: 0.513 ± 0.022
0.296CysPhe: 0.296 ± 0.014
0.657CysGly: 0.657 ± 0.027
0.224CysHis: 0.224 ± 0.012
0.564CysIle: 0.564 ± 0.022
0.417CysLys: 0.417 ± 0.021
0.632CysLeu: 0.632 ± 0.025
0.197CysMet: 0.197 ± 0.013
0.334CysAsn: 0.334 ± 0.017
0.369CysPro: 0.369 ± 0.02
0.249CysGln: 0.249 ± 0.014
0.294CysArg: 0.294 ± 0.019
0.546CysSer: 0.546 ± 0.023
0.379CysThr: 0.379 ± 0.018
0.458CysVal: 0.458 ± 0.021
0.067CysTrp: 0.067 ± 0.008
0.25CysTyr: 0.25 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
3.29AspAla: 3.29 ± 0.057
0.383AspCys: 0.383 ± 0.018
2.795AspAsp: 2.795 ± 0.057
4.547AspGlu: 4.547 ± 0.07
2.363AspPhe: 2.363 ± 0.045
3.33AspGly: 3.33 ± 0.06
0.982AspHis: 0.982 ± 0.028
4.953AspIle: 4.953 ± 0.074
3.331AspLys: 3.331 ± 0.051
4.741AspLeu: 4.741 ± 0.064
1.493AspMet: 1.493 ± 0.034
2.312AspAsn: 2.312 ± 0.051
1.788AspPro: 1.788 ± 0.041
1.629AspGln: 1.629 ± 0.036
2.071AspArg: 2.071 ± 0.046
2.789AspSer: 2.789 ± 0.049
2.809AspThr: 2.809 ± 0.05
4.402AspVal: 4.402 ± 0.068
0.641AspTrp: 0.641 ± 0.025
2.167AspTyr: 2.167 ± 0.036
0.0AspXaa: 0.0 ± 0.0
Glu
5.212GluAla: 5.212 ± 0.072
0.372GluCys: 0.372 ± 0.017
3.912GluAsp: 3.912 ± 0.074
6.025GluGlu: 6.025 ± 0.096
2.523GluPhe: 2.523 ± 0.05
4.04GluGly: 4.04 ± 0.06
1.533GluHis: 1.533 ± 0.038
5.158GluIle: 5.158 ± 0.071
5.812GluLys: 5.812 ± 0.076
7.083GluLeu: 7.083 ± 0.085
1.93GluMet: 1.93 ± 0.044
3.451GluAsn: 3.451 ± 0.061
1.922GluPro: 1.922 ± 0.043
3.627GluGln: 3.627 ± 0.065
3.171GluArg: 3.171 ± 0.053
3.422GluSer: 3.422 ± 0.055
3.774GluThr: 3.774 ± 0.063
4.908GluVal: 4.908 ± 0.07
0.747GluTrp: 0.747 ± 0.029
2.35GluTyr: 2.35 ± 0.043
0.0GluXaa: 0.0 ± 0.0
Phe
3.12PheAla: 3.12 ± 0.057
0.349PheCys: 0.349 ± 0.016
2.36PheAsp: 2.36 ± 0.043
2.85PheGlu: 2.85 ± 0.053
2.409PhePhe: 2.409 ± 0.056
3.129PheGly: 3.129 ± 0.067
0.931PheHis: 0.931 ± 0.029
4.09PheIle: 4.09 ± 0.065
2.466PheLys: 2.466 ± 0.045
4.513PheLeu: 4.513 ± 0.07
1.2PheMet: 1.2 ± 0.039
2.113PheAsn: 2.113 ± 0.044
1.564PhePro: 1.564 ± 0.039
1.499PheGln: 1.499 ± 0.034
1.553PheArg: 1.553 ± 0.037
3.316PheSer: 3.316 ± 0.059
2.773PheThr: 2.773 ± 0.055
3.363PheVal: 3.363 ± 0.059
0.471PheTrp: 0.471 ± 0.021
1.753PheTyr: 1.753 ± 0.036
0.0PheXaa: 0.0 ± 0.0
Gly
4.516GlyAla: 4.516 ± 0.089
0.604GlyCys: 0.604 ± 0.022
3.236GlyAsp: 3.236 ± 0.056
4.035GlyGlu: 4.035 ± 0.066
3.325GlyPhe: 3.325 ± 0.056
4.391GlyGly: 4.391 ± 0.074
1.376GlyHis: 1.376 ± 0.04
5.411GlyIle: 5.411 ± 0.069
4.258GlyLys: 4.258 ± 0.063
6.212GlyLeu: 6.212 ± 0.08
1.894GlyMet: 1.894 ± 0.042
2.557GlyAsn: 2.557 ± 0.05
1.665GlyPro: 1.665 ± 0.041
2.122GlyGln: 2.122 ± 0.044
2.494GlyArg: 2.494 ± 0.047
3.537GlySer: 3.537 ± 0.056
3.953GlyThr: 3.953 ± 0.063
5.067GlyVal: 5.067 ± 0.07
0.747GlyTrp: 0.747 ± 0.026
2.837GlyTyr: 2.837 ± 0.049
0.0GlyXaa: 0.0 ± 0.0
His
1.392HisAla: 1.392 ± 0.033
0.206HisCys: 0.206 ± 0.014
1.252HisAsp: 1.252 ± 0.034
1.527HisGlu: 1.527 ± 0.035
1.014HisPhe: 1.014 ± 0.031
1.311HisGly: 1.311 ± 0.029
0.655HisHis: 0.655 ± 0.024
1.814HisIle: 1.814 ± 0.042
1.128HisLys: 1.128 ± 0.033
1.935HisLeu: 1.935 ± 0.04
0.548HisMet: 0.548 ± 0.019
1.01HisAsn: 1.01 ± 0.027
1.055HisPro: 1.055 ± 0.029
0.699HisGln: 0.699 ± 0.024
0.795HisArg: 0.795 ± 0.026
1.219HisSer: 1.219 ± 0.034
1.271HisThr: 1.271 ± 0.039
1.611HisVal: 1.611 ± 0.035
0.229HisTrp: 0.229 ± 0.014
0.9HisTyr: 0.9 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
6.144IleAla: 6.144 ± 0.083
0.659IleCys: 0.659 ± 0.024
4.706IleAsp: 4.706 ± 0.066
5.868IleGlu: 5.868 ± 0.074
3.509IlePhe: 3.509 ± 0.064
5.58IleGly: 5.58 ± 0.085
1.811IleHis: 1.811 ± 0.038
6.419IleIle: 6.419 ± 0.09
4.673IleLys: 4.673 ± 0.07
7.366IleLeu: 7.366 ± 0.098
1.99IleMet: 1.99 ± 0.043
3.565IleAsn: 3.565 ± 0.055
3.26IlePro: 3.26 ± 0.054
3.071IleGln: 3.071 ± 0.054
3.147IleArg: 3.147 ± 0.058
5.143IleSer: 5.143 ± 0.072
4.834IleThr: 4.834 ± 0.074
6.177IleVal: 6.177 ± 0.082
0.679IleTrp: 0.679 ± 0.023
2.583IleTyr: 2.583 ± 0.057
0.0IleXaa: 0.0 ± 0.0
Lys
4.449LysAla: 4.449 ± 0.067
0.308LysCys: 0.308 ± 0.015
3.701LysAsp: 3.701 ± 0.066
5.661LysGlu: 5.661 ± 0.078
1.815LysPhe: 1.815 ± 0.04
4.061LysGly: 4.061 ± 0.056
1.544LysHis: 1.544 ± 0.037
4.603LysIle: 4.603 ± 0.064
5.243LysLys: 5.243 ± 0.08
5.956LysLeu: 5.956 ± 0.071
1.895LysMet: 1.895 ± 0.042
3.186LysAsn: 3.186 ± 0.051
2.132LysPro: 2.132 ± 0.042
3.631LysGln: 3.631 ± 0.06
3.056LysArg: 3.056 ± 0.054
3.438LysSer: 3.438 ± 0.057
3.407LysThr: 3.407 ± 0.058
4.614LysVal: 4.614 ± 0.066
0.741LysTrp: 0.741 ± 0.025
2.302LysTyr: 2.302 ± 0.051
0.0LysXaa: 0.0 ± 0.0
Leu
7.44LeuAla: 7.44 ± 0.096
0.666LeuCys: 0.666 ± 0.023
4.798LeuAsp: 4.798 ± 0.052
6.315LeuGlu: 6.315 ± 0.083
4.78LeuPhe: 4.78 ± 0.078
5.95LeuGly: 5.95 ± 0.074
2.174LeuHis: 2.174 ± 0.048
7.137LeuIle: 7.137 ± 0.107
6.073LeuLys: 6.073 ± 0.078
10.367LeuLeu: 10.367 ± 0.123
2.362LeuMet: 2.362 ± 0.048
4.177LeuAsn: 4.177 ± 0.059
3.807LeuPro: 3.807 ± 0.059
4.408LeuGln: 4.408 ± 0.064
3.725LeuArg: 3.725 ± 0.054
6.672LeuSer: 6.672 ± 0.067
5.748LeuThr: 5.748 ± 0.072
6.419LeuVal: 6.419 ± 0.076
0.782LeuTrp: 0.782 ± 0.03
3.384LeuTyr: 3.384 ± 0.052
0.0LeuXaa: 0.0 ± 0.0
Met
1.948MetAla: 1.948 ± 0.04
0.175MetCys: 0.175 ± 0.012
1.253MetAsp: 1.253 ± 0.03
1.66MetGlu: 1.66 ± 0.036
1.223MetPhe: 1.223 ± 0.036
1.561MetGly: 1.561 ± 0.036
0.503MetHis: 0.503 ± 0.025
2.032MetIle: 2.032 ± 0.041
2.278MetLys: 2.278 ± 0.046
2.797MetLeu: 2.797 ± 0.055
0.847MetMet: 0.847 ± 0.026
1.556MetAsn: 1.556 ± 0.036
0.997MetPro: 0.997 ± 0.03
1.016MetGln: 1.016 ± 0.031
1.183MetArg: 1.183 ± 0.041
1.709MetSer: 1.709 ± 0.035
1.677MetThr: 1.677 ± 0.034
1.585MetVal: 1.585 ± 0.035
0.207MetTrp: 0.207 ± 0.014
0.898MetTyr: 0.898 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
2.86AsnAla: 2.86 ± 0.055
0.328AsnCys: 0.328 ± 0.018
2.483AsnAsp: 2.483 ± 0.045
3.686AsnGlu: 3.686 ± 0.055
1.717AsnPhe: 1.717 ± 0.036
3.131AsnGly: 3.131 ± 0.053
1.01AsnHis: 1.01 ± 0.031
3.911AsnIle: 3.911 ± 0.061
3.148AsnLys: 3.148 ± 0.054
3.798AsnLeu: 3.798 ± 0.056
1.311AsnMet: 1.311 ± 0.033
2.44AsnAsn: 2.44 ± 0.052
2.066AsnPro: 2.066 ± 0.041
1.79AsnGln: 1.79 ± 0.037
1.987AsnArg: 1.987 ± 0.044
2.538AsnSer: 2.538 ± 0.05
2.445AsnThr: 2.445 ± 0.05
3.361AsnVal: 3.361 ± 0.058
0.557AsnTrp: 0.557 ± 0.02
1.664AsnTyr: 1.664 ± 0.043
0.0AsnXaa: 0.0 ± 0.0
Pro
2.213ProAla: 2.213 ± 0.044
0.237ProCys: 0.237 ± 0.014
1.976ProAsp: 1.976 ± 0.042
2.514ProGlu: 2.514 ± 0.047
2.022ProPhe: 2.022 ± 0.04
2.126ProGly: 2.126 ± 0.047
0.824ProHis: 0.824 ± 0.025
3.017ProIle: 3.017 ± 0.053
1.988ProLys: 1.988 ± 0.041
3.372ProLeu: 3.372 ± 0.053
0.802ProMet: 0.802 ± 0.024
1.82ProAsn: 1.82 ± 0.042
1.007ProPro: 1.007 ± 0.03
1.134ProGln: 1.134 ± 0.029
1.084ProArg: 1.084 ± 0.031
2.156ProSer: 2.156 ± 0.044
2.23ProThr: 2.23 ± 0.037
2.633ProVal: 2.633 ± 0.053
0.377ProTrp: 0.377 ± 0.02
1.501ProTyr: 1.501 ± 0.039
0.0ProXaa: 0.0 ± 0.0
Gln
2.97GlnAla: 2.97 ± 0.053
0.231GlnCys: 0.231 ± 0.016
1.76GlnAsp: 1.76 ± 0.041
2.754GlnGlu: 2.754 ± 0.054
1.719GlnPhe: 1.719 ± 0.035
2.09GlnGly: 2.09 ± 0.04
0.882GlnHis: 0.882 ± 0.03
2.793GlnIle: 2.793 ± 0.051
2.491GlnLys: 2.491 ± 0.043
4.272GlnLeu: 4.272 ± 0.065
1.09GlnMet: 1.09 ± 0.036
1.619GlnAsn: 1.619 ± 0.035
1.321GlnPro: 1.321 ± 0.033
2.133GlnGln: 2.133 ± 0.049
1.524GlnArg: 1.524 ± 0.036
2.269GlnSer: 2.269 ± 0.048
2.275GlnThr: 2.275 ± 0.045
2.559GlnVal: 2.559 ± 0.042
0.391GlnTrp: 0.391 ± 0.017
1.589GlnTyr: 1.589 ± 0.036
0.0GlnXaa: 0.0 ± 0.0
Arg
2.511ArgAla: 2.511 ± 0.051
0.272ArgCys: 0.272 ± 0.016
2.226ArgAsp: 2.226 ± 0.044
2.978ArgGlu: 2.978 ± 0.058
1.838ArgPhe: 1.838 ± 0.04
2.274ArgGly: 2.274 ± 0.041
0.845ArgHis: 0.845 ± 0.026
3.07ArgIle: 3.07 ± 0.056
3.007ArgLys: 3.007 ± 0.057
3.851ArgLeu: 3.851 ± 0.063
1.144ArgMet: 1.144 ± 0.033
1.842ArgAsn: 1.842 ± 0.042
1.284ArgPro: 1.284 ± 0.032
1.604ArgGln: 1.604 ± 0.04
1.778ArgArg: 1.778 ± 0.042
2.087ArgSer: 2.087 ± 0.043
2.013ArgThr: 2.013 ± 0.04
2.722ArgVal: 2.722 ± 0.05
0.398ArgTrp: 0.398 ± 0.019
1.51ArgTyr: 1.51 ± 0.044
0.0ArgXaa: 0.0 ± 0.0
Ser
3.548SerAla: 3.548 ± 0.066
0.452SerCys: 0.452 ± 0.02
2.975SerAsp: 2.975 ± 0.045
3.872SerGlu: 3.872 ± 0.062
3.394SerPhe: 3.394 ± 0.06
3.841SerGly: 3.841 ± 0.054
1.311SerHis: 1.311 ± 0.033
5.493SerIle: 5.493 ± 0.083
3.787SerLys: 3.787 ± 0.05
6.169SerLeu: 6.169 ± 0.079
1.787SerMet: 1.787 ± 0.035
2.756SerAsn: 2.756 ± 0.046
1.991SerPro: 1.991 ± 0.043
2.051SerGln: 2.051 ± 0.049
2.198SerArg: 2.198 ± 0.042
3.938SerSer: 3.938 ± 0.069
3.218SerThr: 3.218 ± 0.055
4.19SerVal: 4.19 ± 0.061
0.656SerTrp: 0.656 ± 0.024
2.416SerTyr: 2.416 ± 0.045
0.0SerXaa: 0.0 ± 0.0
Thr
3.886ThrAla: 3.886 ± 0.072
0.4ThrCys: 0.4 ± 0.017
2.98ThrAsp: 2.98 ± 0.055
3.524ThrGlu: 3.524 ± 0.063
2.924ThrPhe: 2.924 ± 0.054
3.902ThrGly: 3.902 ± 0.058
1.152ThrHis: 1.152 ± 0.03
4.959ThrIle: 4.959 ± 0.072
3.464ThrLys: 3.464 ± 0.058
5.587ThrLeu: 5.587 ± 0.066
1.403ThrMet: 1.403 ± 0.036
2.835ThrAsn: 2.835 ± 0.048
2.287ThrPro: 2.287 ± 0.038
1.636ThrGln: 1.636 ± 0.036
1.949ThrArg: 1.949 ± 0.039
3.495ThrSer: 3.495 ± 0.056
3.392ThrThr: 3.392 ± 0.059
4.513ThrVal: 4.513 ± 0.065
0.578ThrTrp: 0.578 ± 0.022
2.246ThrTyr: 2.246 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
5.282ValAla: 5.282 ± 0.071
0.608ValCys: 0.608 ± 0.026
3.843ValAsp: 3.843 ± 0.058
4.924ValGlu: 4.924 ± 0.076
3.357ValPhe: 3.357 ± 0.053
4.754ValGly: 4.754 ± 0.069
1.378ValHis: 1.378 ± 0.034
5.942ValIle: 5.942 ± 0.079
4.632ValLys: 4.632 ± 0.055
7.065ValLeu: 7.065 ± 0.08
1.93ValMet: 1.93 ± 0.04
3.25ValAsn: 3.25 ± 0.057
2.702ValPro: 2.702 ± 0.047
2.408ValGln: 2.408 ± 0.045
2.66ValArg: 2.66 ± 0.055
4.872ValSer: 4.872 ± 0.056
4.562ValThr: 4.562 ± 0.07
5.59ValVal: 5.59 ± 0.073
0.631ValTrp: 0.631 ± 0.022
2.465ValTyr: 2.465 ± 0.046
0.0ValXaa: 0.0 ± 0.0
Trp
0.63TrpAla: 0.63 ± 0.023
0.077TrpCys: 0.077 ± 0.008
0.546TrpAsp: 0.546 ± 0.021
0.632TrpGlu: 0.632 ± 0.02
0.511TrpPhe: 0.511 ± 0.024
0.684TrpGly: 0.684 ± 0.024
0.24TrpHis: 0.24 ± 0.014
0.803TrpIle: 0.803 ± 0.028
0.643TrpLys: 0.643 ± 0.023
1.098TrpLeu: 1.098 ± 0.03
0.309TrpMet: 0.309 ± 0.017
0.512TrpAsn: 0.512 ± 0.023
0.276TrpPro: 0.276 ± 0.017
0.475TrpGln: 0.475 ± 0.022
0.453TrpArg: 0.453 ± 0.021
0.575TrpSer: 0.575 ± 0.024
0.485TrpThr: 0.485 ± 0.022
0.63TrpVal: 0.63 ± 0.024
0.168TrpTrp: 0.168 ± 0.013
0.349TrpTyr: 0.349 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.248TyrAla: 2.248 ± 0.037
0.337TyrCys: 0.337 ± 0.016
2.186TyrAsp: 2.186 ± 0.045
2.788TyrGlu: 2.788 ± 0.058
1.843TyrPhe: 1.843 ± 0.039
2.518TyrGly: 2.518 ± 0.045
0.843TyrHis: 0.843 ± 0.025
2.894TyrIle: 2.894 ± 0.057
2.338TyrLys: 2.338 ± 0.044
3.239TyrLeu: 3.239 ± 0.052
0.955TyrMet: 0.955 ± 0.026
1.813TyrAsn: 1.813 ± 0.036
1.447TyrPro: 1.447 ± 0.037
1.322TyrGln: 1.322 ± 0.034
1.563TyrArg: 1.563 ± 0.039
2.212TyrSer: 2.212 ± 0.043
1.951TyrThr: 1.951 ± 0.029
2.697TyrVal: 2.697 ± 0.05
0.396TyrTrp: 0.396 ± 0.02
1.542TyrTyr: 1.542 ± 0.039
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4048 proteins (1226140 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski