Aspergillus kawachii (strain NBRC 4308) (White koji mold) (Aspergillus awamori var. kawachi)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus; Aspergillus luchuensis

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11491 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G7XZJ1|G7XZJ1_ASPKW Uncharacterized protein OS=Aspergillus kawachii (strain NBRC 4308) OX=1033177 GN=AKAW_10463 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.34SYY4 pKa = 10.81GIWSTLATLGSFTLSSAKK22 pKa = 9.29TLNFVAHH29 pKa = 7.12EE30 pKa = 4.56DD31 pKa = 3.72DD32 pKa = 5.65DD33 pKa = 5.68LLFLSPDD40 pKa = 4.47LIRR43 pKa = 11.84DD44 pKa = 3.6ILSGEE49 pKa = 4.39PIRR52 pKa = 11.84TVFLTAGDD60 pKa = 3.85AGMGSDD66 pKa = 3.55YY67 pKa = 10.75WNSRR71 pKa = 11.84EE72 pKa = 4.11DD73 pKa = 3.59GSRR76 pKa = 11.84AAYY79 pKa = 10.58ARR81 pKa = 11.84MAVASNNWDD90 pKa = 3.14EE91 pKa = 4.21DD92 pKa = 4.09VIYY95 pKa = 11.19VGDD98 pKa = 3.94KK99 pKa = 10.37TISLYY104 pKa = 10.02TLQDD108 pKa = 3.76DD109 pKa = 5.22DD110 pKa = 5.76DD111 pKa = 5.1ISLAFMHH118 pKa = 6.84LPDD121 pKa = 4.93GNMDD125 pKa = 3.31GTGFGGTDD133 pKa = 3.76QNDD136 pKa = 3.81SLQKK140 pKa = 10.82LWDD143 pKa = 3.55GDD145 pKa = 3.14IDD147 pKa = 4.19VIRR150 pKa = 11.84TIDD153 pKa = 3.56GSGVQYY159 pKa = 10.69TRR161 pKa = 11.84DD162 pKa = 3.58EE163 pKa = 4.42LLDD166 pKa = 4.16TIAALIDD173 pKa = 4.58DD174 pKa = 5.25FDD176 pKa = 5.85PDD178 pKa = 3.61EE179 pKa = 4.89VKK181 pKa = 10.28TGDD184 pKa = 3.6YY185 pKa = 11.43VNDD188 pKa = 4.35FGDD191 pKa = 4.38GDD193 pKa = 4.1HH194 pKa = 7.19SDD196 pKa = 4.11HH197 pKa = 6.56YY198 pKa = 9.99ATGYY202 pKa = 10.41FVDD205 pKa = 4.5NALQQADD212 pKa = 3.87SDD214 pKa = 4.31ADD216 pKa = 3.51LTGYY220 pKa = 10.02YY221 pKa = 10.2GYY223 pKa = 9.34PIQNMDD229 pKa = 3.52VNLDD233 pKa = 3.65DD234 pKa = 6.3SDD236 pKa = 5.69IEE238 pKa = 4.29DD239 pKa = 3.65KK240 pKa = 11.42TNIFYY245 pKa = 10.61EE246 pKa = 4.16YY247 pKa = 10.63AGYY250 pKa = 8.18DD251 pKa = 3.49TATCATDD258 pKa = 3.84DD259 pKa = 3.99ACSSRR264 pKa = 11.84PEE266 pKa = 4.27SAWLQRR272 pKa = 11.84EE273 pKa = 4.34YY274 pKa = 11.09EE275 pKa = 4.28VV276 pKa = 4.24

Molecular weight: 30.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G7Y2E8|G7Y2E8_ASPKW Zinc knuckle domain protein (Fragment) OS=Aspergillus kawachii (strain NBRC 4308) OX=1033177 GN=AKAW_11469 PE=4 SV=1
MM1 pKa = 7.19FVGSGGAAKK10 pKa = 10.21ISAVCPRR17 pKa = 11.84CWLVSLPISLPISLFLPSPSRR38 pKa = 11.84SPTIFRR44 pKa = 11.84APSVGSLSANTPAHH58 pKa = 6.45WGRR61 pKa = 11.84PRR63 pKa = 11.84SQCRR67 pKa = 11.84VRR69 pKa = 11.84SVLNGCIGRR78 pKa = 11.84EE79 pKa = 3.83THH81 pKa = 5.77VRR83 pKa = 11.84SSKK86 pKa = 9.32TLQEE90 pKa = 3.77SRR92 pKa = 11.84ADD94 pKa = 3.69GRR96 pKa = 11.84SLAMTRR102 pKa = 11.84LSEE105 pKa = 4.08EE106 pKa = 4.25CSWRR110 pKa = 11.84LKK112 pKa = 10.11MGLYY116 pKa = 8.32WWKK119 pKa = 11.03KK120 pKa = 7.53MDD122 pKa = 4.39GPTTPLSARR131 pKa = 11.84EE132 pKa = 4.1ASVSVYY138 pKa = 9.53FVHH141 pKa = 7.31WAPCAHH147 pKa = 6.36SVPTLSQPKK156 pKa = 9.32TGLCGNGIEE165 pKa = 4.33VRR167 pKa = 11.84SRR169 pKa = 11.84VAGAFVCRR177 pKa = 11.84WHH179 pKa = 5.93MSSRR183 pKa = 11.84ASTATDD189 pKa = 3.57LQAPRR194 pKa = 11.84RR195 pKa = 11.84QPEE198 pKa = 4.09LHH200 pKa = 6.71CSTRR204 pKa = 11.84LLLGGCRR211 pKa = 11.84ARR213 pKa = 11.84ASSTGFPAALYY224 pKa = 10.7SDD226 pKa = 4.92ADD228 pKa = 3.43QFTRR232 pKa = 11.84SSAVDD237 pKa = 3.1AHH239 pKa = 5.91TAGAVVFRR247 pKa = 11.84IPRR250 pKa = 11.84PLWHH254 pKa = 6.88IPVPPADD261 pKa = 3.29WLGPGDD267 pKa = 4.11SLVGG271 pKa = 3.57

Molecular weight: 29.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11488

3

11491

5648345

48

6297

491.5

54.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.504 ± 0.021

1.246 ± 0.009

5.673 ± 0.015

6.194 ± 0.024

3.709 ± 0.014

6.791 ± 0.022

2.433 ± 0.011

4.956 ± 0.015

4.458 ± 0.019

9.12 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.181 ± 0.008

3.611 ± 0.011

6.015 ± 0.028

4.041 ± 0.017

6.064 ± 0.019

8.253 ± 0.025

6.063 ± 0.015

6.265 ± 0.016

1.496 ± 0.008

2.926 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski