Mycobacterium phage Thonko

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bclasvirinae; unclassified Bclasvirinae

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 108 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346FCB2|A0A346FCB2_9CAUD Uncharacterized protein OS=Mycobacterium phage Thonko OX=2282910 GN=65 PE=4 SV=1
MM1 pKa = 7.35TRR3 pKa = 11.84TPLAVLAGAVVLGCGIGYY21 pKa = 10.14AIVGCDD27 pKa = 3.09AAAPDD32 pKa = 5.29DD33 pKa = 3.95IAVPMPAASGEE44 pKa = 4.08PFDD47 pKa = 3.83FAEE50 pKa = 4.29YY51 pKa = 11.01AEE53 pKa = 5.51AITGTDD59 pKa = 3.03RR60 pKa = 11.84AEE62 pKa = 3.8WQPASVYY69 pKa = 11.14DD70 pKa = 4.55LMLGPPPRR78 pKa = 11.84IEE80 pKa = 3.8EE81 pKa = 4.91DD82 pKa = 3.56EE83 pKa = 4.82PGWNCATMGDD93 pKa = 4.29RR94 pKa = 11.84NCGPEE99 pKa = 4.1LPTDD103 pKa = 3.55VFDD106 pKa = 5.31EE107 pKa = 4.21LRR109 pKa = 11.84SWVAAGGSPDD119 pKa = 3.43YY120 pKa = 10.34TLNADD125 pKa = 3.71GTITGPQGVIIRR137 pKa = 11.84LPRR140 pKa = 4.71

Molecular weight: 14.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346FC72|A0A346FC72_9CAUD Tail assembly chaperone OS=Mycobacterium phage Thonko OX=2282910 GN=25 PE=4 SV=1
MM1 pKa = 7.27ARR3 pKa = 11.84SRR5 pKa = 11.84GRR7 pKa = 11.84GSTRR11 pKa = 11.84RR12 pKa = 11.84GTRR15 pKa = 11.84RR16 pKa = 11.84GLAKK20 pKa = 10.24SSPRR24 pKa = 11.84IAQRR28 pKa = 11.84RR29 pKa = 11.84GSAVALSASTGGVGRR44 pKa = 11.84HH45 pKa = 5.14TGVGAFGRR53 pKa = 11.84RR54 pKa = 11.84GGYY57 pKa = 9.88RR58 pKa = 11.84GFKK61 pKa = 10.2SKK63 pKa = 10.65KK64 pKa = 5.56QWKK67 pKa = 7.72WAFASGQPWARR78 pKa = 11.84EE79 pKa = 3.88KK80 pKa = 10.81AHH82 pKa = 5.96KK83 pKa = 9.16TKK85 pKa = 10.63GGPKK89 pKa = 8.92VRR91 pKa = 11.84YY92 pKa = 9.16RR93 pKa = 11.84RR94 pKa = 11.84LPASKK99 pKa = 10.07HH100 pKa = 4.82SGRR103 pKa = 11.84KK104 pKa = 8.73GRR106 pKa = 11.84RR107 pKa = 11.84TPSS110 pKa = 2.8

Molecular weight: 12.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

108

0

108

21953

29

2095

203.3

22.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.176 ± 0.333

1.07 ± 0.174

6.824 ± 0.245

6.263 ± 0.261

2.542 ± 0.105

9.078 ± 0.554

1.859 ± 0.162

4.733 ± 0.149

2.852 ± 0.173

7.94 ± 0.204

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.032 ± 0.088

2.619 ± 0.163

6.291 ± 0.179

3.321 ± 0.282

7.466 ± 0.371

4.765 ± 0.161

6.518 ± 0.229

7.516 ± 0.194

1.89 ± 0.123

2.246 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski