Amino acid dipepetide frequency for Mycobacterium phage Thonko

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.039AlaAla: 18.039 ± 1.252
0.774AlaCys: 0.774 ± 0.22
8.473AlaAsp: 8.473 ± 0.73
10.25AlaGlu: 10.25 ± 0.814
2.597AlaPhe: 2.597 ± 0.319
7.972AlaGly: 7.972 ± 0.961
2.688AlaHis: 2.688 ± 0.383
5.876AlaIle: 5.876 ± 0.516
3.28AlaLys: 3.28 ± 0.447
11.206AlaLeu: 11.206 ± 0.768
2.915AlaMet: 2.915 ± 0.437
2.597AlaAsn: 2.597 ± 0.395
6.286AlaPro: 6.286 ± 0.513
4.419AlaGln: 4.419 ± 0.418
8.837AlaArg: 8.837 ± 0.763
4.191AlaSer: 4.191 ± 0.383
7.38AlaThr: 7.38 ± 0.491
10.067AlaVal: 10.067 ± 0.639
1.685AlaTrp: 1.685 ± 0.27
2.232AlaTyr: 2.232 ± 0.374
0.0AlaXaa: 0.0 ± 0.0
Cys
0.774CysAla: 0.774 ± 0.181
0.091CysCys: 0.091 ± 0.063
1.002CysAsp: 1.002 ± 0.269
0.729CysGlu: 0.729 ± 0.175
0.137CysPhe: 0.137 ± 0.096
1.503CysGly: 1.503 ± 0.296
0.228CysHis: 0.228 ± 0.099
0.364CysIle: 0.364 ± 0.106
0.319CysLys: 0.319 ± 0.125
0.774CysLeu: 0.774 ± 0.164
0.137CysMet: 0.137 ± 0.074
0.456CysAsn: 0.456 ± 0.161
0.547CysPro: 0.547 ± 0.173
0.319CysGln: 0.319 ± 0.116
1.184CysArg: 1.184 ± 0.278
0.41CysSer: 0.41 ± 0.147
0.456CysThr: 0.456 ± 0.121
0.866CysVal: 0.866 ± 0.235
0.228CysTrp: 0.228 ± 0.118
0.182CysTyr: 0.182 ± 0.084
0.0CysXaa: 0.0 ± 0.0
Asp
7.699AspAla: 7.699 ± 0.758
0.638AspCys: 0.638 ± 0.208
5.375AspAsp: 5.375 ± 0.55
4.419AspGlu: 4.419 ± 0.558
2.05AspPhe: 2.05 ± 0.275
6.56AspGly: 6.56 ± 0.533
1.822AspHis: 1.822 ± 0.275
3.325AspIle: 3.325 ± 0.322
1.822AspLys: 1.822 ± 0.3
5.375AspLeu: 5.375 ± 0.502
1.868AspMet: 1.868 ± 0.268
1.23AspAsn: 1.23 ± 0.224
5.603AspPro: 5.603 ± 0.694
2.141AspGln: 2.141 ± 0.379
4.419AspArg: 4.419 ± 0.49
2.733AspSer: 2.733 ± 0.395
3.918AspThr: 3.918 ± 0.37
4.464AspVal: 4.464 ± 0.39
1.002AspTrp: 1.002 ± 0.209
1.777AspTyr: 1.777 ± 0.322
0.0AspXaa: 0.0 ± 0.0
Glu
8.473GluAla: 8.473 ± 0.788
0.547GluCys: 0.547 ± 0.161
3.325GluAsp: 3.325 ± 0.656
2.05GluGlu: 2.05 ± 0.293
1.777GluPhe: 1.777 ± 0.273
5.512GluGly: 5.512 ± 0.451
1.685GluHis: 1.685 ± 0.304
3.052GluIle: 3.052 ± 0.368
1.549GluLys: 1.549 ± 0.273
5.74GluLeu: 5.74 ± 0.558
0.729GluMet: 0.729 ± 0.174
0.957GluAsn: 0.957 ± 0.182
3.963GluPro: 3.963 ± 0.419
3.553GluGln: 3.553 ± 0.517
5.33GluArg: 5.33 ± 0.568
2.323GluSer: 2.323 ± 0.359
3.371GluThr: 3.371 ± 0.377
5.603GluVal: 5.603 ± 0.501
1.685GluTrp: 1.685 ± 0.266
1.412GluTyr: 1.412 ± 0.247
0.0GluXaa: 0.0 ± 0.0
Phe
3.508PheAla: 3.508 ± 0.331
0.364PheCys: 0.364 ± 0.123
3.098PheAsp: 3.098 ± 0.399
0.683PheGlu: 0.683 ± 0.157
0.501PhePhe: 0.501 ± 0.166
2.824PheGly: 2.824 ± 0.382
0.547PheHis: 0.547 ± 0.184
1.002PheIle: 1.002 ± 0.199
0.683PheLys: 0.683 ± 0.149
1.822PheLeu: 1.822 ± 0.288
0.547PheMet: 0.547 ± 0.148
0.957PheAsn: 0.957 ± 0.204
1.184PhePro: 1.184 ± 0.177
0.41PheGln: 0.41 ± 0.129
1.594PheArg: 1.594 ± 0.336
0.683PheSer: 0.683 ± 0.136
2.232PheThr: 2.232 ± 0.316
1.868PheVal: 1.868 ± 0.254
0.364PheTrp: 0.364 ± 0.11
0.547PheTyr: 0.547 ± 0.19
0.0PheXaa: 0.0 ± 0.0
Gly
7.744GlyAla: 7.744 ± 0.765
0.866GlyCys: 0.866 ± 0.206
6.378GlyAsp: 6.378 ± 0.663
5.33GlyGlu: 5.33 ± 0.527
2.323GlyPhe: 2.323 ± 0.33
9.749GlyGly: 9.749 ± 1.846
1.549GlyHis: 1.549 ± 0.319
4.1GlyIle: 4.1 ± 0.409
3.918GlyLys: 3.918 ± 0.432
7.243GlyLeu: 7.243 ± 0.761
1.777GlyMet: 1.777 ± 0.317
2.141GlyAsn: 2.141 ± 0.276
4.328GlyPro: 4.328 ± 0.664
3.827GlyGln: 3.827 ± 0.534
5.922GlyArg: 5.922 ± 0.624
4.601GlySer: 4.601 ± 0.5
6.742GlyThr: 6.742 ± 0.616
7.106GlyVal: 7.106 ± 0.499
2.323GlyTrp: 2.323 ± 0.434
2.824GlyTyr: 2.824 ± 0.345
0.0GlyXaa: 0.0 ± 0.0
His
2.05HisAla: 2.05 ± 0.343
0.091HisCys: 0.091 ± 0.067
1.367HisAsp: 1.367 ± 0.275
0.683HisGlu: 0.683 ± 0.156
0.547HisPhe: 0.547 ± 0.183
1.321HisGly: 1.321 ± 0.265
0.501HisHis: 0.501 ± 0.164
0.957HisIle: 0.957 ± 0.183
0.41HisLys: 0.41 ± 0.121
1.321HisLeu: 1.321 ± 0.237
0.137HisMet: 0.137 ± 0.081
0.592HisAsn: 0.592 ± 0.186
1.321HisPro: 1.321 ± 0.252
0.547HisGln: 0.547 ± 0.178
2.414HisArg: 2.414 ± 0.469
0.866HisSer: 0.866 ± 0.16
1.412HisThr: 1.412 ± 0.291
0.957HisVal: 0.957 ± 0.207
0.501HisTrp: 0.501 ± 0.141
0.592HisTyr: 0.592 ± 0.155
0.0HisXaa: 0.0 ± 0.0
Ile
7.289IleAla: 7.289 ± 0.588
0.638IleCys: 0.638 ± 0.255
4.419IleAsp: 4.419 ± 0.395
5.831IleGlu: 5.831 ± 0.504
0.82IlePhe: 0.82 ± 0.166
5.33IleGly: 5.33 ± 0.43
0.319IleHis: 0.319 ± 0.1
2.323IleIle: 2.323 ± 0.321
1.503IleLys: 1.503 ± 0.272
1.959IleLeu: 1.959 ± 0.291
0.774IleMet: 0.774 ± 0.183
1.913IleAsn: 1.913 ± 0.247
1.777IlePro: 1.777 ± 0.293
0.364IleGln: 0.364 ± 0.119
2.46IleArg: 2.46 ± 0.287
1.685IleSer: 1.685 ± 0.286
3.007IleThr: 3.007 ± 0.352
3.371IleVal: 3.371 ± 0.454
0.501IleTrp: 0.501 ± 0.129
1.048IleTyr: 1.048 ± 0.226
0.0IleXaa: 0.0 ± 0.0
Lys
3.644LysAla: 3.644 ± 0.454
0.182LysCys: 0.182 ± 0.101
1.139LysAsp: 1.139 ± 0.258
0.774LysGlu: 0.774 ± 0.184
0.592LysPhe: 0.592 ± 0.168
2.551LysGly: 2.551 ± 0.342
0.456LysHis: 0.456 ± 0.144
1.412LysIle: 1.412 ± 0.279
0.273LysLys: 0.273 ± 0.124
3.325LysLeu: 3.325 ± 0.341
0.456LysMet: 0.456 ± 0.135
0.456LysAsn: 0.456 ± 0.147
1.913LysPro: 1.913 ± 0.351
1.048LysGln: 1.048 ± 0.22
2.278LysArg: 2.278 ± 0.397
1.685LysSer: 1.685 ± 0.227
1.959LysThr: 1.959 ± 0.325
2.824LysVal: 2.824 ± 0.4
0.82LysTrp: 0.82 ± 0.193
0.729LysTyr: 0.729 ± 0.166
0.0LysXaa: 0.0 ± 0.0
Leu
11.844LeuAla: 11.844 ± 0.929
0.638LeuCys: 0.638 ± 0.167
6.651LeuAsp: 6.651 ± 0.59
2.551LeuGlu: 2.551 ± 0.326
2.187LeuPhe: 2.187 ± 0.358
7.835LeuGly: 7.835 ± 0.651
1.503LeuHis: 1.503 ± 0.324
3.781LeuIle: 3.781 ± 0.478
1.685LeuLys: 1.685 ± 0.34
6.56LeuLeu: 6.56 ± 0.537
1.64LeuMet: 1.64 ± 0.257
3.007LeuAsn: 3.007 ± 0.443
5.694LeuPro: 5.694 ± 0.484
1.868LeuGln: 1.868 ± 0.331
5.375LeuArg: 5.375 ± 0.706
3.781LeuSer: 3.781 ± 0.406
5.649LeuThr: 5.649 ± 0.599
4.783LeuVal: 4.783 ± 0.378
1.23LeuTrp: 1.23 ± 0.195
1.139LeuTyr: 1.139 ± 0.271
0.0LeuXaa: 0.0 ± 0.0
Met
1.777MetAla: 1.777 ± 0.223
0.182MetCys: 0.182 ± 0.086
0.638MetAsp: 0.638 ± 0.132
0.501MetGlu: 0.501 ± 0.152
0.456MetPhe: 0.456 ± 0.123
1.23MetGly: 1.23 ± 0.239
0.501MetHis: 0.501 ± 0.133
1.139MetIle: 1.139 ± 0.223
0.683MetLys: 0.683 ± 0.195
1.913MetLeu: 1.913 ± 0.297
0.501MetMet: 0.501 ± 0.14
0.364MetAsn: 0.364 ± 0.108
1.913MetPro: 1.913 ± 0.316
0.638MetGln: 0.638 ± 0.138
1.594MetArg: 1.594 ± 0.266
1.959MetSer: 1.959 ± 0.291
3.098MetThr: 3.098 ± 0.333
0.592MetVal: 0.592 ± 0.148
0.319MetTrp: 0.319 ± 0.149
0.319MetTyr: 0.319 ± 0.117
0.0MetXaa: 0.0 ± 0.0
Asn
2.961AsnAla: 2.961 ± 0.461
0.364AsnCys: 0.364 ± 0.121
1.139AsnAsp: 1.139 ± 0.241
1.594AsnGlu: 1.594 ± 0.277
0.638AsnPhe: 0.638 ± 0.206
2.369AsnGly: 2.369 ± 0.288
0.456AsnHis: 0.456 ± 0.128
1.23AsnIle: 1.23 ± 0.212
1.002AsnLys: 1.002 ± 0.22
2.323AsnLeu: 2.323 ± 0.424
0.41AsnMet: 0.41 ± 0.133
0.683AsnAsn: 0.683 ± 0.306
2.05AsnPro: 2.05 ± 0.322
0.456AsnGln: 0.456 ± 0.151
1.64AsnArg: 1.64 ± 0.269
1.822AsnSer: 1.822 ± 0.259
1.731AsnThr: 1.731 ± 0.312
2.095AsnVal: 2.095 ± 0.31
0.638AsnTrp: 0.638 ± 0.17
0.592AsnTyr: 0.592 ± 0.188
0.0AsnXaa: 0.0 ± 0.0
Pro
5.603ProAla: 5.603 ± 0.479
0.547ProCys: 0.547 ± 0.173
4.92ProAsp: 4.92 ± 0.515
5.649ProGlu: 5.649 ± 0.503
1.777ProPhe: 1.777 ± 0.266
6.97ProGly: 6.97 ± 0.782
1.23ProHis: 1.23 ± 0.266
2.688ProIle: 2.688 ± 0.375
1.594ProLys: 1.594 ± 0.246
4.1ProLeu: 4.1 ± 0.421
1.184ProMet: 1.184 ± 0.214
1.23ProAsn: 1.23 ± 0.205
5.284ProPro: 5.284 ± 0.42
2.141ProGln: 2.141 ± 0.342
3.143ProArg: 3.143 ± 0.422
3.462ProSer: 3.462 ± 0.419
3.508ProThr: 3.508 ± 0.448
6.013ProVal: 6.013 ± 0.476
1.002ProTrp: 1.002 ± 0.23
0.866ProTyr: 0.866 ± 0.207
0.0ProXaa: 0.0 ± 0.0
Gln
4.874GlnAla: 4.874 ± 0.641
0.319GlnCys: 0.319 ± 0.135
0.911GlnAsp: 0.911 ± 0.23
1.002GlnGlu: 1.002 ± 0.236
1.139GlnPhe: 1.139 ± 0.262
2.505GlnGly: 2.505 ± 0.41
0.41GlnHis: 0.41 ± 0.13
2.232GlnIle: 2.232 ± 0.346
1.048GlnLys: 1.048 ± 0.216
2.597GlnLeu: 2.597 ± 0.337
1.23GlnMet: 1.23 ± 0.22
0.683GlnAsn: 0.683 ± 0.189
2.597GlnPro: 2.597 ± 0.374
1.139GlnGln: 1.139 ± 0.378
3.508GlnArg: 3.508 ± 0.455
1.458GlnSer: 1.458 ± 0.277
2.141GlnThr: 2.141 ± 0.371
2.187GlnVal: 2.187 ± 0.223
0.683GlnTrp: 0.683 ± 0.184
0.547GlnTyr: 0.547 ± 0.157
0.0GlnXaa: 0.0 ± 0.0
Arg
8.017ArgAla: 8.017 ± 0.572
1.276ArgCys: 1.276 ± 0.312
4.738ArgAsp: 4.738 ± 0.498
5.694ArgGlu: 5.694 ± 0.53
1.503ArgPhe: 1.503 ± 0.248
5.375ArgGly: 5.375 ± 0.583
1.64ArgHis: 1.64 ± 0.277
3.325ArgIle: 3.325 ± 0.405
2.187ArgLys: 2.187 ± 0.363
5.831ArgLeu: 5.831 ± 0.582
1.777ArgMet: 1.777 ± 0.246
2.414ArgAsn: 2.414 ± 0.279
3.69ArgPro: 3.69 ± 0.442
2.733ArgGln: 2.733 ± 0.314
7.608ArgArg: 7.608 ± 0.806
4.1ArgSer: 4.1 ± 0.429
4.419ArgThr: 4.419 ± 0.442
4.464ArgVal: 4.464 ± 0.539
1.822ArgTrp: 1.822 ± 0.335
2.004ArgTyr: 2.004 ± 0.414
0.0ArgXaa: 0.0 ± 0.0
Ser
5.466SerAla: 5.466 ± 0.462
0.729SerCys: 0.729 ± 0.204
2.961SerAsp: 2.961 ± 0.393
2.323SerGlu: 2.323 ± 0.318
1.048SerPhe: 1.048 ± 0.181
4.829SerGly: 4.829 ± 0.501
0.729SerHis: 0.729 ± 0.2
2.05SerIle: 2.05 ± 0.307
1.594SerLys: 1.594 ± 0.232
3.644SerLeu: 3.644 ± 0.444
1.048SerMet: 1.048 ± 0.226
1.321SerAsn: 1.321 ± 0.265
3.827SerPro: 3.827 ± 0.449
1.458SerGln: 1.458 ± 0.261
3.827SerArg: 3.827 ± 0.442
1.868SerSer: 1.868 ± 0.405
2.642SerThr: 2.642 ± 0.297
4.145SerVal: 4.145 ± 0.512
0.911SerTrp: 0.911 ± 0.199
1.23SerTyr: 1.23 ± 0.217
0.0SerXaa: 0.0 ± 0.0
Thr
8.427ThrAla: 8.427 ± 0.678
0.729ThrCys: 0.729 ± 0.212
4.555ThrAsp: 4.555 ± 0.443
4.054ThrGlu: 4.054 ± 0.498
2.004ThrPhe: 2.004 ± 0.238
6.423ThrGly: 6.423 ± 0.621
0.774ThrHis: 0.774 ± 0.177
3.28ThrIle: 3.28 ± 0.471
1.822ThrLys: 1.822 ± 0.255
4.237ThrLeu: 4.237 ± 0.383
1.23ThrMet: 1.23 ± 0.226
1.549ThrAsn: 1.549 ± 0.324
4.054ThrPro: 4.054 ± 0.49
1.777ThrGln: 1.777 ± 0.37
4.464ThrArg: 4.464 ± 0.473
3.781ThrSer: 3.781 ± 0.544
4.145ThrThr: 4.145 ± 0.556
5.284ThrVal: 5.284 ± 0.423
0.82ThrTrp: 0.82 ± 0.198
1.777ThrTyr: 1.777 ± 0.298
0.0ThrXaa: 0.0 ± 0.0
Val
8.564ValAla: 8.564 ± 0.581
0.82ValCys: 0.82 ± 0.212
4.692ValAsp: 4.692 ± 0.429
5.831ValGlu: 5.831 ± 0.578
1.959ValPhe: 1.959 ± 0.24
7.152ValGly: 7.152 ± 0.611
0.683ValHis: 0.683 ± 0.173
3.644ValIle: 3.644 ± 0.366
2.232ValLys: 2.232 ± 0.299
6.241ValLeu: 6.241 ± 0.651
1.367ValMet: 1.367 ± 0.225
2.414ValAsn: 2.414 ± 0.329
4.738ValPro: 4.738 ± 0.535
2.414ValGln: 2.414 ± 0.39
4.601ValArg: 4.601 ± 0.538
3.963ValSer: 3.963 ± 0.438
5.239ValThr: 5.239 ± 0.561
5.558ValVal: 5.558 ± 0.488
1.367ValTrp: 1.367 ± 0.283
1.685ValTyr: 1.685 ± 0.286
0.0ValXaa: 0.0 ± 0.0
Trp
2.232TrpAla: 2.232 ± 0.373
0.547TrpCys: 0.547 ± 0.164
1.23TrpAsp: 1.23 ± 0.228
0.729TrpGlu: 0.729 ± 0.169
0.592TrpPhe: 0.592 ± 0.223
0.729TrpGly: 0.729 ± 0.19
0.638TrpHis: 0.638 ± 0.185
0.683TrpIle: 0.683 ± 0.14
0.592TrpLys: 0.592 ± 0.141
1.458TrpLeu: 1.458 ± 0.285
0.273TrpMet: 0.273 ± 0.119
0.729TrpAsn: 0.729 ± 0.215
1.048TrpPro: 1.048 ± 0.198
1.093TrpGln: 1.093 ± 0.239
1.594TrpArg: 1.594 ± 0.216
1.458TrpSer: 1.458 ± 0.248
0.866TrpThr: 0.866 ± 0.2
1.458TrpVal: 1.458 ± 0.294
0.456TrpTrp: 0.456 ± 0.158
0.501TrpTyr: 0.501 ± 0.169
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.779TyrAla: 2.779 ± 0.398
0.364TyrCys: 0.364 ± 0.143
1.23TyrAsp: 1.23 ± 0.244
1.959TyrGlu: 1.959 ± 0.284
0.774TyrPhe: 0.774 ± 0.174
2.05TyrGly: 2.05 ± 0.308
0.228TyrHis: 0.228 ± 0.121
0.866TyrIle: 0.866 ± 0.221
0.319TyrLys: 0.319 ± 0.12
1.731TyrLeu: 1.731 ± 0.343
0.273TyrMet: 0.273 ± 0.105
0.501TyrAsn: 0.501 ± 0.128
1.093TyrPro: 1.093 ± 0.275
0.866TyrGln: 0.866 ± 0.174
2.87TyrArg: 2.87 ± 0.416
0.82TyrSer: 0.82 ± 0.197
1.276TyrThr: 1.276 ± 0.197
1.458TyrVal: 1.458 ± 0.246
0.547TyrTrp: 0.547 ± 0.134
0.456TyrTyr: 0.456 ± 0.149
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 108 proteins (21953 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski