Amino acid dipepetide frequency for Streptomyces sp. TSRI0107

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.713AlaAla: 21.713 ± 0.164
1.067AlaCys: 1.067 ± 0.022
8.611AlaAsp: 8.611 ± 0.08
9.273AlaGlu: 9.273 ± 0.089
3.541AlaPhe: 3.541 ± 0.042
13.219AlaGly: 13.219 ± 0.1
2.911AlaHis: 2.911 ± 0.039
3.287AlaIle: 3.287 ± 0.04
2.67AlaLys: 2.67 ± 0.049
14.836AlaLeu: 14.836 ± 0.115
2.407AlaMet: 2.407 ± 0.032
1.848AlaAsn: 1.848 ± 0.031
7.286AlaPro: 7.286 ± 0.075
3.66AlaGln: 3.66 ± 0.049
10.669AlaArg: 10.669 ± 0.089
5.645AlaSer: 5.645 ± 0.052
6.873AlaThr: 6.873 ± 0.061
12.675AlaVal: 12.675 ± 0.086
1.948AlaTrp: 1.948 ± 0.031
2.824AlaTyr: 2.824 ± 0.034
0.0AlaXaa: 0.0 ± 0.0
Cys
1.077CysAla: 1.077 ± 0.022
0.089CysCys: 0.089 ± 0.008
0.474CysAsp: 0.474 ± 0.015
0.408CysGlu: 0.408 ± 0.014
0.211CysPhe: 0.211 ± 0.009
0.936CysGly: 0.936 ± 0.02
0.193CysHis: 0.193 ± 0.008
0.129CysIle: 0.129 ± 0.007
0.103CysLys: 0.103 ± 0.007
0.755CysLeu: 0.755 ± 0.017
0.112CysMet: 0.112 ± 0.007
0.127CysAsn: 0.127 ± 0.007
0.475CysPro: 0.475 ± 0.016
0.154CysGln: 0.154 ± 0.007
0.594CysArg: 0.594 ± 0.016
0.399CysSer: 0.399 ± 0.012
0.498CysThr: 0.498 ± 0.015
0.684CysVal: 0.684 ± 0.019
0.131CysTrp: 0.131 ± 0.008
0.14CysTyr: 0.14 ± 0.007
0.0CysXaa: 0.0 ± 0.0
Asp
7.885AspAla: 7.885 ± 0.061
0.429AspCys: 0.429 ± 0.014
3.831AspAsp: 3.831 ± 0.051
4.065AspGlu: 4.065 ± 0.039
1.675AspPhe: 1.675 ± 0.03
6.769AspGly: 6.769 ± 0.071
1.408AspHis: 1.408 ± 0.026
1.881AspIle: 1.881 ± 0.029
1.181AspLys: 1.181 ± 0.031
6.212AspLeu: 6.212 ± 0.058
0.81AspMet: 0.81 ± 0.019
0.912AspAsn: 0.912 ± 0.023
4.688AspPro: 4.688 ± 0.051
1.446AspGln: 1.446 ± 0.024
5.137AspArg: 5.137 ± 0.051
2.495AspSer: 2.495 ± 0.035
3.298AspThr: 3.298 ± 0.036
4.886AspVal: 4.886 ± 0.045
1.107AspTrp: 1.107 ± 0.025
1.125AspTyr: 1.125 ± 0.024
0.0AspXaa: 0.0 ± 0.0
Glu
7.675GluAla: 7.675 ± 0.082
0.371GluCys: 0.371 ± 0.014
3.016GluAsp: 3.016 ± 0.038
3.823GluGlu: 3.823 ± 0.05
1.523GluPhe: 1.523 ± 0.026
4.409GluGly: 4.409 ± 0.049
1.581GluHis: 1.581 ± 0.023
2.209GluIle: 2.209 ± 0.035
1.461GluLys: 1.461 ± 0.033
6.996GluLeu: 6.996 ± 0.064
0.869GluMet: 0.869 ± 0.017
1.051GluAsn: 1.051 ± 0.024
3.618GluPro: 3.618 ± 0.045
2.331GluGln: 2.331 ± 0.035
5.74GluArg: 5.74 ± 0.056
2.51GluSer: 2.51 ± 0.032
3.013GluThr: 3.013 ± 0.034
4.508GluVal: 4.508 ± 0.051
0.791GluTrp: 0.791 ± 0.02
1.143GluTyr: 1.143 ± 0.026
0.0GluXaa: 0.0 ± 0.0
Phe
3.619PheAla: 3.619 ± 0.041
0.254PheCys: 0.254 ± 0.01
1.959PheAsp: 1.959 ± 0.036
1.46PheGlu: 1.46 ± 0.024
0.829PhePhe: 0.829 ± 0.021
3.003PheGly: 3.003 ± 0.045
0.63PheHis: 0.63 ± 0.017
0.631PheIle: 0.631 ± 0.017
0.506PheLys: 0.506 ± 0.016
2.618PheLeu: 2.618 ± 0.041
0.393PheMet: 0.393 ± 0.014
0.527PheAsn: 0.527 ± 0.014
1.397PhePro: 1.397 ± 0.025
0.707PheGln: 0.707 ± 0.018
1.905PheArg: 1.905 ± 0.03
1.373PheSer: 1.373 ± 0.025
2.043PheThr: 2.043 ± 0.028
2.238PheVal: 2.238 ± 0.033
0.43PheTrp: 0.43 ± 0.014
0.562PheTyr: 0.562 ± 0.015
0.0PheXaa: 0.0 ± 0.0
Gly
10.996GlyAla: 10.996 ± 0.083
0.822GlyCys: 0.822 ± 0.019
5.411GlyAsp: 5.411 ± 0.047
5.294GlyGlu: 5.294 ± 0.05
2.864GlyPhe: 2.864 ± 0.037
8.839GlyGly: 8.839 ± 0.082
2.367GlyHis: 2.367 ± 0.038
3.328GlyIle: 3.328 ± 0.039
2.326GlyLys: 2.326 ± 0.038
9.567GlyLeu: 9.567 ± 0.074
1.998GlyMet: 1.998 ± 0.031
1.736GlyAsn: 1.736 ± 0.034
5.124GlyPro: 5.124 ± 0.048
2.573GlyGln: 2.573 ± 0.04
7.923GlyArg: 7.923 ± 0.066
5.126GlySer: 5.126 ± 0.056
6.604GlyThr: 6.604 ± 0.056
7.726GlyVal: 7.726 ± 0.062
1.731GlyTrp: 1.731 ± 0.03
2.311GlyTyr: 2.311 ± 0.034
0.0GlyXaa: 0.0 ± 0.0
His
2.782HisAla: 2.782 ± 0.04
0.212HisCys: 0.212 ± 0.011
1.39HisAsp: 1.39 ± 0.025
1.211HisGlu: 1.211 ± 0.023
0.652HisPhe: 0.652 ± 0.019
2.476HisGly: 2.476 ± 0.034
0.693HisHis: 0.693 ± 0.018
0.661HisIle: 0.661 ± 0.018
0.347HisLys: 0.347 ± 0.012
2.452HisLeu: 2.452 ± 0.036
0.331HisMet: 0.331 ± 0.011
0.359HisAsn: 0.359 ± 0.013
1.85HisPro: 1.85 ± 0.033
0.633HisGln: 0.633 ± 0.016
2.161HisArg: 2.161 ± 0.033
0.982HisSer: 0.982 ± 0.022
1.367HisThr: 1.367 ± 0.024
1.679HisVal: 1.679 ± 0.028
0.377HisTrp: 0.377 ± 0.013
0.47HisTyr: 0.47 ± 0.015
0.0HisXaa: 0.0 ± 0.0
Ile
4.624IleAla: 4.624 ± 0.054
0.269IleCys: 0.269 ± 0.011
2.143IleAsp: 2.143 ± 0.034
1.889IleGlu: 1.889 ± 0.029
0.643IlePhe: 0.643 ± 0.018
3.369IleGly: 3.369 ± 0.043
0.571IleHis: 0.571 ± 0.017
0.788IleIle: 0.788 ± 0.021
0.67IleLys: 0.67 ± 0.018
2.284IleLeu: 2.284 ± 0.036
0.418IleMet: 0.418 ± 0.015
0.624IleAsn: 0.624 ± 0.016
1.728IlePro: 1.728 ± 0.031
0.666IleGln: 0.666 ± 0.018
2.198IleArg: 2.198 ± 0.034
1.552IleSer: 1.552 ± 0.028
2.142IleThr: 2.142 ± 0.034
2.673IleVal: 2.673 ± 0.038
0.362IleTrp: 0.362 ± 0.011
0.49IleTyr: 0.49 ± 0.017
0.0IleXaa: 0.0 ± 0.0
Lys
2.84LysAla: 2.84 ± 0.038
0.105LysCys: 0.105 ± 0.007
1.348LysAsp: 1.348 ± 0.033
1.212LysGlu: 1.212 ± 0.03
0.433LysPhe: 0.433 ± 0.015
1.775LysGly: 1.775 ± 0.03
0.386LysHis: 0.386 ± 0.013
0.777LysIle: 0.777 ± 0.02
0.791LysLys: 0.791 ± 0.029
1.954LysLeu: 1.954 ± 0.037
0.387LysMet: 0.387 ± 0.014
0.468LysAsn: 0.468 ± 0.016
1.329LysPro: 1.329 ± 0.029
0.651LysGln: 0.651 ± 0.019
1.396LysArg: 1.396 ± 0.029
1.065LysSer: 1.065 ± 0.025
1.219LysThr: 1.219 ± 0.033
1.853LysVal: 1.853 ± 0.038
0.251LysTrp: 0.251 ± 0.013
0.433LysTyr: 0.433 ± 0.013
0.0LysXaa: 0.0 ± 0.0
Leu
15.515LeuAla: 15.515 ± 0.111
0.838LeuCys: 0.838 ± 0.019
6.839LeuAsp: 6.839 ± 0.058
4.882LeuGlu: 4.882 ± 0.053
2.618LeuPhe: 2.618 ± 0.035
9.379LeuGly: 9.379 ± 0.069
2.332LeuHis: 2.332 ± 0.034
3.203LeuIle: 3.203 ± 0.048
2.021LeuLys: 2.021 ± 0.037
11.623LeuLeu: 11.623 ± 0.101
1.621LeuMet: 1.621 ± 0.028
1.64LeuAsn: 1.64 ± 0.026
6.602LeuPro: 6.602 ± 0.055
2.146LeuGln: 2.146 ± 0.033
8.893LeuArg: 8.893 ± 0.081
5.08LeuSer: 5.08 ± 0.046
7.107LeuThr: 7.107 ± 0.058
8.836LeuVal: 8.836 ± 0.069
1.323LeuTrp: 1.323 ± 0.025
1.913LeuTyr: 1.913 ± 0.028
0.0LeuXaa: 0.0 ± 0.0
Met
2.235MetAla: 2.235 ± 0.03
0.129MetCys: 0.129 ± 0.008
0.882MetAsp: 0.882 ± 0.018
0.747MetGlu: 0.747 ± 0.021
0.433MetPhe: 0.433 ± 0.014
1.298MetGly: 1.298 ± 0.028
0.333MetHis: 0.333 ± 0.011
0.623MetIle: 0.623 ± 0.017
0.403MetLys: 0.403 ± 0.012
1.605MetLeu: 1.605 ± 0.027
0.282MetMet: 0.282 ± 0.011
0.409MetAsn: 0.409 ± 0.013
1.091MetPro: 1.091 ± 0.021
0.425MetGln: 0.425 ± 0.015
1.397MetArg: 1.397 ± 0.023
1.276MetSer: 1.276 ± 0.025
1.522MetThr: 1.522 ± 0.025
1.183MetVal: 1.183 ± 0.022
0.211MetTrp: 0.211 ± 0.01
0.323MetTyr: 0.323 ± 0.01
0.0MetXaa: 0.0 ± 0.0
Asn
2.121AsnAla: 2.121 ± 0.032
0.154AsnCys: 0.154 ± 0.008
0.913AsnAsp: 0.913 ± 0.022
0.809AsnGlu: 0.809 ± 0.019
0.465AsnPhe: 0.465 ± 0.014
1.837AsnGly: 1.837 ± 0.037
0.39AsnHis: 0.39 ± 0.013
0.595AsnIle: 0.595 ± 0.018
0.393AsnLys: 0.393 ± 0.014
1.58AsnLeu: 1.58 ± 0.026
0.284AsnMet: 0.284 ± 0.011
0.413AsnAsn: 0.413 ± 0.017
1.319AsnPro: 1.319 ± 0.028
0.497AsnGln: 0.497 ± 0.014
1.215AsnArg: 1.215 ± 0.023
0.847AsnSer: 0.847 ± 0.022
1.089AsnThr: 1.089 ± 0.026
1.366AsnVal: 1.366 ± 0.024
0.291AsnTrp: 0.291 ± 0.011
0.399AsnTyr: 0.399 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
8.982ProAla: 8.982 ± 0.083
0.328ProCys: 0.328 ± 0.013
4.694ProAsp: 4.694 ± 0.053
4.651ProGlu: 4.651 ± 0.051
1.561ProPhe: 1.561 ± 0.026
6.768ProGly: 6.768 ± 0.063
1.42ProHis: 1.42 ± 0.029
1.225ProIle: 1.225 ± 0.025
1.119ProLys: 1.119 ± 0.026
5.485ProLeu: 5.485 ± 0.051
0.945ProMet: 0.945 ± 0.02
0.888ProAsn: 0.888 ± 0.022
3.605ProPro: 3.605 ± 0.054
1.748ProGln: 1.748 ± 0.036
4.07ProArg: 4.07 ± 0.049
3.057ProSer: 3.057 ± 0.037
3.078ProThr: 3.078 ± 0.038
5.596ProVal: 5.596 ± 0.054
0.937ProTrp: 0.937 ± 0.023
1.495ProTyr: 1.495 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
3.767GlnAla: 3.767 ± 0.051
0.157GlnCys: 0.157 ± 0.008
1.41GlnAsp: 1.41 ± 0.027
1.444GlnGlu: 1.444 ± 0.026
0.626GlnPhe: 0.626 ± 0.016
2.291GlnGly: 2.291 ± 0.039
0.629GlnHis: 0.629 ± 0.016
1.0GlnIle: 1.0 ± 0.023
0.564GlnLys: 0.564 ± 0.017
2.918GlnLeu: 2.918 ± 0.033
0.465GlnMet: 0.465 ± 0.015
0.487GlnAsn: 0.487 ± 0.016
1.734GlnPro: 1.734 ± 0.04
1.183GlnGln: 1.183 ± 0.032
2.309GlnArg: 2.309 ± 0.028
1.176GlnSer: 1.176 ± 0.023
1.301GlnThr: 1.301 ± 0.024
2.268GlnVal: 2.268 ± 0.032
0.467GlnTrp: 0.467 ± 0.013
0.591GlnTyr: 0.591 ± 0.017
0.0GlnXaa: 0.0 ± 0.0
Arg
10.229ArgAla: 10.229 ± 0.089
0.554ArgCys: 0.554 ± 0.015
4.446ArgAsp: 4.446 ± 0.048
4.933ArgGlu: 4.933 ± 0.057
2.371ArgPhe: 2.371 ± 0.034
5.845ArgGly: 5.845 ± 0.049
2.23ArgHis: 2.23 ± 0.035
3.114ArgIle: 3.114 ± 0.041
1.582ArgLys: 1.582 ± 0.029
9.384ArgLeu: 9.384 ± 0.084
1.706ArgMet: 1.706 ± 0.03
1.291ArgAsn: 1.291 ± 0.026
5.153ArgPro: 5.153 ± 0.056
2.329ArgGln: 2.329 ± 0.034
8.054ArgArg: 8.054 ± 0.084
3.833ArgSer: 3.833 ± 0.044
5.42ArgThr: 5.42 ± 0.049
6.163ArgVal: 6.163 ± 0.054
1.394ArgTrp: 1.394 ± 0.027
1.87ArgTyr: 1.87 ± 0.027
0.0ArgXaa: 0.0 ± 0.0
Ser
6.491SerAla: 6.491 ± 0.063
0.391SerCys: 0.391 ± 0.013
2.545SerAsp: 2.545 ± 0.036
2.346SerGlu: 2.346 ± 0.033
1.429SerPhe: 1.429 ± 0.023
5.606SerGly: 5.606 ± 0.061
0.99SerHis: 0.99 ± 0.023
1.261SerIle: 1.261 ± 0.026
0.953SerLys: 0.953 ± 0.024
4.749SerLeu: 4.749 ± 0.051
0.997SerMet: 0.997 ± 0.021
0.83SerAsn: 0.83 ± 0.021
3.036SerPro: 3.036 ± 0.04
1.147SerGln: 1.147 ± 0.026
3.545SerArg: 3.545 ± 0.042
2.529SerSer: 2.529 ± 0.04
2.849SerThr: 2.849 ± 0.04
4.157SerVal: 4.157 ± 0.044
0.847SerTrp: 0.847 ± 0.019
1.203SerTyr: 1.203 ± 0.024
0.0SerXaa: 0.0 ± 0.0
Thr
9.135ThrAla: 9.135 ± 0.074
0.428ThrCys: 0.428 ± 0.013
3.738ThrAsp: 3.738 ± 0.039
3.316ThrGlu: 3.316 ± 0.038
1.549ThrPhe: 1.549 ± 0.026
6.887ThrGly: 6.887 ± 0.057
1.226ThrHis: 1.226 ± 0.024
1.633ThrIle: 1.633 ± 0.035
1.128ThrLys: 1.128 ± 0.025
5.741ThrLeu: 5.741 ± 0.046
0.876ThrMet: 0.876 ± 0.018
0.96ThrAsn: 0.96 ± 0.023
4.321ThrPro: 4.321 ± 0.053
1.279ThrGln: 1.279 ± 0.022
3.983ThrArg: 3.983 ± 0.043
2.984ThrSer: 2.984 ± 0.042
3.77ThrThr: 3.77 ± 0.046
6.147ThrVal: 6.147 ± 0.055
0.906ThrTrp: 0.906 ± 0.019
1.369ThrTyr: 1.369 ± 0.025
0.0ThrXaa: 0.0 ± 0.0
Val
10.716ValAla: 10.716 ± 0.073
0.767ValCys: 0.767 ± 0.018
5.094ValAsp: 5.094 ± 0.049
4.746ValGlu: 4.746 ± 0.055
2.495ValPhe: 2.495 ± 0.039
6.474ValGly: 6.474 ± 0.059
2.023ValHis: 2.023 ± 0.031
2.748ValIle: 2.748 ± 0.042
1.713ValLys: 1.713 ± 0.033
9.749ValLeu: 9.749 ± 0.065
1.405ValMet: 1.405 ± 0.024
1.611ValAsn: 1.611 ± 0.029
5.426ValPro: 5.426 ± 0.059
1.99ValGln: 1.99 ± 0.029
7.494ValArg: 7.494 ± 0.067
4.146ValSer: 4.146 ± 0.042
5.895ValThr: 5.895 ± 0.058
8.127ValVal: 8.127 ± 0.069
1.229ValTrp: 1.229 ± 0.027
1.64ValTyr: 1.64 ± 0.03
0.0ValXaa: 0.0 ± 0.0
Trp
1.708TrpAla: 1.708 ± 0.029
0.147TrpCys: 0.147 ± 0.008
0.852TrpAsp: 0.852 ± 0.021
0.776TrpGlu: 0.776 ± 0.017
0.523TrpPhe: 0.523 ± 0.016
1.094TrpGly: 1.094 ± 0.026
0.378TrpHis: 0.378 ± 0.014
0.551TrpIle: 0.551 ± 0.015
0.357TrpLys: 0.357 ± 0.014
1.849TrpLeu: 1.849 ± 0.029
0.265TrpMet: 0.265 ± 0.009
0.412TrpAsn: 0.412 ± 0.013
0.818TrpPro: 0.818 ± 0.019
0.636TrpGln: 0.636 ± 0.017
1.381TrpArg: 1.381 ± 0.027
0.902TrpSer: 0.902 ± 0.021
1.084TrpThr: 1.084 ± 0.023
1.005TrpVal: 1.005 ± 0.021
0.355TrpTrp: 0.355 ± 0.014
0.359TrpTyr: 0.359 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.851TyrAla: 2.851 ± 0.033
0.174TyrCys: 0.174 ± 0.01
1.645TyrAsp: 1.645 ± 0.03
1.354TyrGlu: 1.354 ± 0.024
0.646TyrPhe: 0.646 ± 0.018
2.392TyrGly: 2.392 ± 0.033
0.394TyrHis: 0.394 ± 0.013
0.457TyrIle: 0.457 ± 0.013
0.389TyrLys: 0.389 ± 0.013
2.051TyrLeu: 2.051 ± 0.03
0.236TyrMet: 0.236 ± 0.01
0.385TyrAsn: 0.385 ± 0.016
1.054TyrPro: 1.054 ± 0.024
0.587TyrGln: 0.587 ± 0.018
1.837TyrArg: 1.837 ± 0.03
0.92TyrSer: 0.92 ± 0.021
1.202TyrThr: 1.202 ± 0.022
1.72TyrVal: 1.72 ± 0.031
0.361TyrTrp: 0.361 ± 0.012
0.455TyrTyr: 0.455 ± 0.016
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7155 proteins (2402202 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski