Eubacterium uniforme

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2409 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1T4W1Q3|A0A1T4W1Q3_9FIRM Uncharacterized protein OS=Eubacterium uniforme OX=39495 GN=SAMN02745111_02153 PE=3 SV=1
MM1 pKa = 7.93YY2 pKa = 10.48EE3 pKa = 4.57EE4 pKa = 5.14ILMACKK10 pKa = 9.75EE11 pKa = 4.51WIYY14 pKa = 10.67NIQILYY20 pKa = 10.4ADD22 pKa = 4.61KK23 pKa = 11.13LDD25 pKa = 4.1IDD27 pKa = 4.27IEE29 pKa = 4.21VDD31 pKa = 3.13NAKK34 pKa = 10.36EE35 pKa = 3.74LVVNIDD41 pKa = 3.05SDD43 pKa = 3.9NYY45 pKa = 10.75LGQLCISGPDD55 pKa = 3.32FRR57 pKa = 11.84PYY59 pKa = 11.13NFVEE63 pKa = 4.42FTILDD68 pKa = 3.55INQDD72 pKa = 3.26IGKK75 pKa = 9.21VPAFWYY81 pKa = 10.52GDD83 pKa = 3.62KK84 pKa = 10.75EE85 pKa = 4.17GNAAQEE91 pKa = 4.61IIDD94 pKa = 3.91NLNIGLEE101 pKa = 4.12LLLQEE106 pKa = 4.62

Molecular weight: 12.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1T4VEV6|A0A1T4VEV6_9FIRM tRNA modification GTPase MnmE OS=Eubacterium uniforme OX=39495 GN=mnmE PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.61KK9 pKa = 7.54RR10 pKa = 11.84QRR12 pKa = 11.84SRR14 pKa = 11.84VHH16 pKa = 6.29GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSVGGRR28 pKa = 11.84KK29 pKa = 8.97VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.61GRR39 pKa = 11.84KK40 pKa = 8.87KK41 pKa = 10.59LSAA44 pKa = 3.95

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2409

0

2409

853700

40

3521

354.4

40.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.026 ± 0.054

1.33 ± 0.018

6.765 ± 0.046

7.598 ± 0.051

4.233 ± 0.04

6.538 ± 0.052

1.357 ± 0.018

8.339 ± 0.044

9.275 ± 0.059

7.797 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.708 ± 0.028

5.881 ± 0.057

2.591 ± 0.026

2.015 ± 0.022

3.424 ± 0.035

6.157 ± 0.044

5.258 ± 0.051

7.117 ± 0.038

0.756 ± 0.014

4.837 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski