Amino acid dipepetide frequency for Staphylococcus aureus (strain NCTC 8325 / PS 47)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.077AlaAla: 4.077 ± 0.12
0.487AlaCys: 0.487 ± 0.026
3.105AlaAsp: 3.105 ± 0.083
3.542AlaGlu: 3.542 ± 0.082
3.007AlaPhe: 3.007 ± 0.073
3.903AlaGly: 3.903 ± 0.095
1.436AlaHis: 1.436 ± 0.046
6.213AlaIle: 6.213 ± 0.117
4.848AlaLys: 4.848 ± 0.154
6.6AlaLeu: 6.6 ± 0.136
1.841AlaMet: 1.841 ± 0.067
3.152AlaAsn: 3.152 ± 0.121
1.869AlaPro: 1.869 ± 0.053
2.755AlaGln: 2.755 ± 0.115
2.152AlaArg: 2.152 ± 0.063
3.9AlaSer: 3.9 ± 0.093
3.959AlaThr: 3.959 ± 0.14
4.362AlaVal: 4.362 ± 0.089
0.451AlaTrp: 0.451 ± 0.025
2.349AlaTyr: 2.349 ± 0.059
0.0AlaXaa: 0.0 ± 0.0
Cys
0.349CysAla: 0.349 ± 0.021
0.089CysCys: 0.089 ± 0.009
0.356CysAsp: 0.356 ± 0.021
0.334CysGlu: 0.334 ± 0.021
0.285CysPhe: 0.285 ± 0.02
0.581CysGly: 0.581 ± 0.027
0.219CysHis: 0.219 ± 0.017
0.573CysIle: 0.573 ± 0.029
0.371CysLys: 0.371 ± 0.025
0.636CysLeu: 0.636 ± 0.034
0.139CysMet: 0.139 ± 0.014
0.297CysAsn: 0.297 ± 0.018
0.257CysPro: 0.257 ± 0.021
0.199CysGln: 0.199 ± 0.017
0.235CysArg: 0.235 ± 0.018
0.403CysSer: 0.403 ± 0.023
0.382CysThr: 0.382 ± 0.021
0.398CysVal: 0.398 ± 0.024
0.053CysTrp: 0.053 ± 0.009
0.288CysTyr: 0.288 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
3.693AspAla: 3.693 ± 0.117
0.352AspCys: 0.352 ± 0.024
3.5AspAsp: 3.5 ± 0.087
4.438AspGlu: 4.438 ± 0.08
2.686AspPhe: 2.686 ± 0.054
3.212AspGly: 3.212 ± 0.068
1.141AspHis: 1.141 ± 0.038
5.356AspIle: 5.356 ± 0.081
4.873AspLys: 4.873 ± 0.114
5.046AspLeu: 5.046 ± 0.093
1.531AspMet: 1.531 ± 0.047
3.073AspAsn: 3.073 ± 0.069
1.487AspPro: 1.487 ± 0.049
2.144AspGln: 2.144 ± 0.085
1.855AspArg: 1.855 ± 0.053
3.503AspSer: 3.503 ± 0.289
2.971AspThr: 2.971 ± 0.069
4.199AspVal: 4.199 ± 0.075
0.47AspTrp: 0.47 ± 0.028
2.586AspTyr: 2.586 ± 0.074
0.0AspXaa: 0.0 ± 0.0
Glu
4.556GluAla: 4.556 ± 0.095
0.314GluCys: 0.314 ± 0.02
3.737GluAsp: 3.737 ± 0.083
4.713GluGlu: 4.713 ± 0.124
2.336GluPhe: 2.336 ± 0.064
3.279GluGly: 3.279 ± 0.082
1.614GluHis: 1.614 ± 0.052
5.206GluIle: 5.206 ± 0.102
5.399GluLys: 5.399 ± 0.114
5.885GluLeu: 5.885 ± 0.106
1.958GluMet: 1.958 ± 0.053
3.91GluAsn: 3.91 ± 0.074
1.713GluPro: 1.713 ± 0.051
3.288GluGln: 3.288 ± 0.074
2.862GluArg: 2.862 ± 0.072
3.525GluSer: 3.525 ± 0.09
3.649GluThr: 3.649 ± 0.071
4.347GluVal: 4.347 ± 0.101
0.485GluTrp: 0.485 ± 0.024
2.21GluTyr: 2.21 ± 0.062
0.0GluXaa: 0.0 ± 0.0
Phe
2.576PheAla: 2.576 ± 0.065
0.321PheCys: 0.321 ± 0.021
2.807PheAsp: 2.807 ± 0.059
3.061PheGlu: 3.061 ± 0.069
1.996PhePhe: 1.996 ± 0.061
3.124PheGly: 3.124 ± 0.067
0.795PheHis: 0.795 ± 0.035
4.57PheIle: 4.57 ± 0.118
3.691PheLys: 3.691 ± 0.077
3.949PheLeu: 3.949 ± 0.104
1.116PheMet: 1.116 ± 0.041
2.909PheAsn: 2.909 ± 0.062
1.216PhePro: 1.216 ± 0.043
1.268PheGln: 1.268 ± 0.036
1.2PheArg: 1.2 ± 0.039
2.861PheSer: 2.861 ± 0.065
2.414PheThr: 2.414 ± 0.054
3.155PheVal: 3.155 ± 0.084
0.364PheTrp: 0.364 ± 0.021
1.812PheTyr: 1.812 ± 0.053
0.0PheXaa: 0.0 ± 0.0
Gly
4.12GlyAla: 4.12 ± 0.099
0.446GlyCys: 0.446 ± 0.024
3.054GlyAsp: 3.054 ± 0.061
3.361GlyGlu: 3.361 ± 0.09
3.05GlyPhe: 3.05 ± 0.074
4.135GlyGly: 4.135 ± 0.105
1.457GlyHis: 1.457 ± 0.044
5.426GlyIle: 5.426 ± 0.094
4.442GlyLys: 4.442 ± 0.1
5.72GlyLeu: 5.72 ± 0.103
1.62GlyMet: 1.62 ± 0.052
2.711GlyAsn: 2.711 ± 0.079
1.559GlyPro: 1.559 ± 0.058
2.29GlyGln: 2.29 ± 0.058
2.153GlyArg: 2.153 ± 0.061
3.542GlySer: 3.542 ± 0.085
3.481GlyThr: 3.481 ± 0.08
4.475GlyVal: 4.475 ± 0.08
0.525GlyTrp: 0.525 ± 0.03
2.753GlyTyr: 2.753 ± 0.061
0.0GlyXaa: 0.0 ± 0.0
His
1.413HisAla: 1.413 ± 0.05
0.162HisCys: 0.162 ± 0.014
1.378HisAsp: 1.378 ± 0.047
1.262HisGlu: 1.262 ± 0.042
1.272HisPhe: 1.272 ± 0.044
1.347HisGly: 1.347 ± 0.042
0.779HisHis: 0.779 ± 0.036
1.944HisIle: 1.944 ± 0.05
1.293HisLys: 1.293 ± 0.046
2.241HisLeu: 2.241 ± 0.056
0.588HisMet: 0.588 ± 0.03
1.071HisAsn: 1.071 ± 0.035
1.028HisPro: 1.028 ± 0.037
1.19HisGln: 1.19 ± 0.037
0.875HisArg: 0.875 ± 0.033
1.235HisSer: 1.235 ± 0.041
1.175HisThr: 1.175 ± 0.041
1.549HisVal: 1.549 ± 0.045
0.174HisTrp: 0.174 ± 0.016
1.023HisTyr: 1.023 ± 0.038
0.0HisXaa: 0.0 ± 0.0
Ile
6.199IleAla: 6.199 ± 0.105
0.716IleCys: 0.716 ± 0.032
5.524IleAsp: 5.524 ± 0.096
5.952IleGlu: 5.952 ± 0.111
3.839IlePhe: 3.839 ± 0.103
5.673IleGly: 5.673 ± 0.121
1.729IleHis: 1.729 ± 0.051
7.489IleIle: 7.489 ± 0.152
6.186IleLys: 6.186 ± 0.11
7.443IleLeu: 7.443 ± 0.161
2.04IleMet: 2.04 ± 0.068
4.995IleAsn: 4.995 ± 0.115
3.093IlePro: 3.093 ± 0.071
2.993IleGln: 2.993 ± 0.063
2.581IleArg: 2.581 ± 0.07
5.598IleSer: 5.598 ± 0.105
4.903IleThr: 4.903 ± 0.086
6.076IleVal: 6.076 ± 0.133
0.525IleTrp: 0.525 ± 0.032
3.015IleTyr: 3.015 ± 0.075
0.0IleXaa: 0.0 ± 0.0
Lys
4.882LysAla: 4.882 ± 0.118
0.325LysCys: 0.325 ± 0.021
5.359LysAsp: 5.359 ± 0.103
6.061LysGlu: 6.061 ± 0.115
2.445LysPhe: 2.445 ± 0.059
4.24LysGly: 4.24 ± 0.089
1.899LysHis: 1.899 ± 0.049
5.065LysIle: 5.065 ± 0.097
5.795LysLys: 5.795 ± 0.128
6.332LysLeu: 6.332 ± 0.104
2.245LysMet: 2.245 ± 0.058
4.362LysAsn: 4.362 ± 0.088
2.403LysPro: 2.403 ± 0.077
3.74LysGln: 3.74 ± 0.134
3.094LysArg: 3.094 ± 0.069
4.433LysSer: 4.433 ± 0.096
3.856LysThr: 3.856 ± 0.074
5.035LysVal: 5.035 ± 0.081
0.617LysTrp: 0.617 ± 0.034
3.279LysTyr: 3.279 ± 0.081
0.0LysXaa: 0.0 ± 0.0
Leu
5.882LeuAla: 5.882 ± 0.089
0.626LeuCys: 0.626 ± 0.033
4.714LeuAsp: 4.714 ± 0.09
5.424LeuGlu: 5.424 ± 0.096
4.495LeuPhe: 4.495 ± 0.117
5.489LeuGly: 5.489 ± 0.11
1.655LeuHis: 1.655 ± 0.042
8.011LeuIle: 8.011 ± 0.163
6.995LeuLys: 6.995 ± 0.102
8.639LeuLeu: 8.639 ± 0.178
2.459LeuMet: 2.459 ± 0.063
5.918LeuAsn: 5.918 ± 0.145
3.406LeuPro: 3.406 ± 0.066
3.172LeuGln: 3.172 ± 0.076
3.014LeuArg: 3.014 ± 0.058
6.455LeuSer: 6.455 ± 0.128
5.778LeuThr: 5.778 ± 0.083
5.589LeuVal: 5.589 ± 0.115
0.579LeuTrp: 0.579 ± 0.031
3.118LeuTyr: 3.118 ± 0.08
0.0LeuXaa: 0.0 ± 0.0
Met
1.776MetAla: 1.776 ± 0.058
0.172MetCys: 0.172 ± 0.015
1.239MetAsp: 1.239 ± 0.044
1.292MetGlu: 1.292 ± 0.043
1.109MetPhe: 1.109 ± 0.043
1.457MetGly: 1.457 ± 0.05
0.535MetHis: 0.535 ± 0.026
2.502MetIle: 2.502 ± 0.07
2.183MetLys: 2.183 ± 0.057
2.403MetLeu: 2.403 ± 0.063
0.912MetMet: 0.912 ± 0.048
1.729MetAsn: 1.729 ± 0.039
0.953MetPro: 0.953 ± 0.038
0.91MetGln: 0.91 ± 0.035
0.962MetArg: 0.962 ± 0.034
1.971MetSer: 1.971 ± 0.074
2.029MetThr: 2.029 ± 0.05
1.476MetVal: 1.476 ± 0.045
0.167MetTrp: 0.167 ± 0.013
0.869MetTyr: 0.869 ± 0.034
0.0MetXaa: 0.0 ± 0.0
Asn
3.643AsnAla: 3.643 ± 0.229
0.281AsnCys: 0.281 ± 0.016
3.712AsnAsp: 3.712 ± 0.076
4.131AsnGlu: 4.131 ± 0.086
2.113AsnPhe: 2.113 ± 0.046
3.508AsnGly: 3.508 ± 0.1
1.603AsnHis: 1.603 ± 0.045
4.832AsnIle: 4.832 ± 0.098
4.702AsnLys: 4.702 ± 0.09
4.199AsnLeu: 4.199 ± 0.085
1.397AsnMet: 1.397 ± 0.043
3.52AsnAsn: 3.52 ± 0.121
2.103AsnPro: 2.103 ± 0.069
2.849AsnGln: 2.849 ± 0.106
1.968AsnArg: 1.968 ± 0.049
2.763AsnSer: 2.763 ± 0.079
2.856AsnThr: 2.856 ± 0.111
3.83AsnVal: 3.83 ± 0.07
0.477AsnTrp: 0.477 ± 0.028
2.363AsnTyr: 2.363 ± 0.059
0.0AsnXaa: 0.0 ± 0.0
Pro
1.667ProAla: 1.667 ± 0.061
0.154ProCys: 0.154 ± 0.015
1.648ProAsp: 1.648 ± 0.054
2.471ProGlu: 2.471 ± 0.065
1.775ProPhe: 1.775 ± 0.056
1.85ProGly: 1.85 ± 0.066
0.785ProHis: 0.785 ± 0.033
2.72ProIle: 2.72 ± 0.064
2.464ProLys: 2.464 ± 0.069
2.894ProLeu: 2.894 ± 0.068
0.773ProMet: 0.773 ± 0.036
1.949ProAsn: 1.949 ± 0.06
0.72ProPro: 0.72 ± 0.033
1.245ProGln: 1.245 ± 0.039
0.943ProArg: 0.943 ± 0.038
2.023ProSer: 2.023 ± 0.049
2.089ProThr: 2.089 ± 0.064
2.32ProVal: 2.32 ± 0.06
0.257ProTrp: 0.257 ± 0.022
1.371ProTyr: 1.371 ± 0.043
0.0ProXaa: 0.0 ± 0.0
Gln
2.899GlnAla: 2.899 ± 0.194
0.231GlnCys: 0.231 ± 0.017
2.131GlnAsp: 2.131 ± 0.049
2.117GlnGlu: 2.117 ± 0.054
1.975GlnPhe: 1.975 ± 0.048
2.157GlnGly: 2.157 ± 0.054
1.19GlnHis: 1.19 ± 0.045
3.174GlnIle: 3.174 ± 0.072
2.582GlnLys: 2.582 ± 0.069
4.269GlnLeu: 4.269 ± 0.094
1.119GlnMet: 1.119 ± 0.044
2.328GlnAsn: 2.328 ± 0.114
1.316GlnPro: 1.316 ± 0.044
2.577GlnGln: 2.577 ± 0.085
1.711GlnArg: 1.711 ± 0.049
2.701GlnSer: 2.701 ± 0.073
2.271GlnThr: 2.271 ± 0.084
2.415GlnVal: 2.415 ± 0.082
0.349GlnTrp: 0.349 ± 0.02
1.752GlnTyr: 1.752 ± 0.053
0.0GlnXaa: 0.0 ± 0.0
Arg
2.017ArgAla: 2.017 ± 0.057
0.213ArgCys: 0.213 ± 0.019
1.956ArgAsp: 1.956 ± 0.052
2.359ArgGlu: 2.359 ± 0.063
1.691ArgPhe: 1.691 ± 0.039
1.93ArgGly: 1.93 ± 0.055
0.897ArgHis: 0.897 ± 0.031
2.943ArgIle: 2.943 ± 0.059
2.696ArgLys: 2.696 ± 0.066
3.31ArgLeu: 3.31 ± 0.069
1.037ArgMet: 1.037 ± 0.04
1.886ArgAsn: 1.886 ± 0.056
1.13ArgPro: 1.13 ± 0.04
1.757ArgGln: 1.757 ± 0.05
1.606ArgArg: 1.606 ± 0.047
1.741ArgSer: 1.741 ± 0.043
1.847ArgThr: 1.847 ± 0.06
2.191ArgVal: 2.191 ± 0.054
0.275ArgTrp: 0.275 ± 0.018
1.539ArgTyr: 1.539 ± 0.048
0.0ArgXaa: 0.0 ± 0.0
Ser
3.396SerAla: 3.396 ± 0.081
0.336SerCys: 0.336 ± 0.019
3.987SerAsp: 3.987 ± 0.274
3.885SerGlu: 3.885 ± 0.105
2.935SerPhe: 2.935 ± 0.062
3.846SerGly: 3.846 ± 0.094
1.452SerHis: 1.452 ± 0.044
5.376SerIle: 5.376 ± 0.097
4.758SerLys: 4.758 ± 0.091
5.627SerLeu: 5.627 ± 0.126
1.61SerMet: 1.61 ± 0.063
3.588SerAsn: 3.588 ± 0.078
1.718SerPro: 1.718 ± 0.048
2.414SerGln: 2.414 ± 0.068
2.082SerArg: 2.082 ± 0.052
3.703SerSer: 3.703 ± 0.096
3.692SerThr: 3.692 ± 0.289
3.787SerVal: 3.787 ± 0.089
0.456SerTrp: 0.456 ± 0.025
2.402SerTyr: 2.402 ± 0.061
0.0SerXaa: 0.0 ± 0.0
Thr
3.776ThrAla: 3.776 ± 0.153
0.347ThrCys: 0.347 ± 0.023
2.992ThrAsp: 2.992 ± 0.068
3.399ThrGlu: 3.399 ± 0.079
2.819ThrPhe: 2.819 ± 0.064
3.628ThrGly: 3.628 ± 0.08
1.351ThrHis: 1.351 ± 0.047
5.143ThrIle: 5.143 ± 0.088
3.996ThrLys: 3.996 ± 0.08
5.597ThrLeu: 5.597 ± 0.084
1.396ThrMet: 1.396 ± 0.042
3.143ThrAsn: 3.143 ± 0.097
2.348ThrPro: 2.348 ± 0.073
2.027ThrGln: 2.027 ± 0.082
1.78ThrArg: 1.78 ± 0.051
3.753ThrSer: 3.753 ± 0.277
3.741ThrThr: 3.741 ± 0.119
3.984ThrVal: 3.984 ± 0.094
0.488ThrTrp: 0.488 ± 0.023
2.257ThrTyr: 2.257 ± 0.052
0.0ThrXaa: 0.0 ± 0.0
Val
4.441ValAla: 4.441 ± 0.092
0.505ValCys: 0.505 ± 0.022
4.011ValAsp: 4.011 ± 0.078
4.37ValGlu: 4.37 ± 0.091
2.869ValPhe: 2.869 ± 0.08
4.141ValGly: 4.141 ± 0.089
1.229ValHis: 1.229 ± 0.043
6.155ValIle: 6.155 ± 0.113
4.95ValLys: 4.95 ± 0.08
6.018ValLeu: 6.018 ± 0.112
1.719ValMet: 1.719 ± 0.053
3.723ValAsn: 3.723 ± 0.092
2.3ValPro: 2.3 ± 0.06
2.162ValGln: 2.162 ± 0.059
2.132ValArg: 2.132 ± 0.062
4.312ValSer: 4.312 ± 0.075
4.314ValThr: 4.314 ± 0.105
4.787ValVal: 4.787 ± 0.087
0.463ValTrp: 0.463 ± 0.03
2.213ValTyr: 2.213 ± 0.055
0.0ValXaa: 0.0 ± 0.0
Trp
0.395TrpAla: 0.395 ± 0.026
0.066TrpCys: 0.066 ± 0.009
0.336TrpAsp: 0.336 ± 0.022
0.384TrpGlu: 0.384 ± 0.021
0.438TrpPhe: 0.438 ± 0.025
0.459TrpGly: 0.459 ± 0.031
0.171TrpHis: 0.171 ± 0.013
0.681TrpIle: 0.681 ± 0.033
0.472TrpLys: 0.472 ± 0.021
0.933TrpLeu: 0.933 ± 0.042
0.253TrpMet: 0.253 ± 0.016
0.423TrpAsn: 0.423 ± 0.028
0.221TrpPro: 0.221 ± 0.019
0.346TrpGln: 0.346 ± 0.02
0.28TrpArg: 0.28 ± 0.022
0.455TrpSer: 0.455 ± 0.03
0.411TrpThr: 0.411 ± 0.021
0.444TrpVal: 0.444 ± 0.024
0.075TrpTrp: 0.075 ± 0.01
0.322TrpTyr: 0.322 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.251TyrAla: 2.251 ± 0.06
0.3TyrCys: 0.3 ± 0.021
2.572TyrAsp: 2.572 ± 0.068
2.433TyrGlu: 2.433 ± 0.059
2.072TyrPhe: 2.072 ± 0.065
2.361TyrGly: 2.361 ± 0.064
1.062TyrHis: 1.062 ± 0.034
3.192TyrIle: 3.192 ± 0.073
2.77TyrLys: 2.77 ± 0.073
3.676TyrLeu: 3.676 ± 0.087
0.892TyrMet: 0.892 ± 0.034
2.161TyrAsn: 2.161 ± 0.065
1.278TyrPro: 1.278 ± 0.042
1.935TyrGln: 1.935 ± 0.052
1.539TyrArg: 1.539 ± 0.047
2.175TyrSer: 2.175 ± 0.054
2.143TyrThr: 2.143 ± 0.059
2.397TyrVal: 2.397 ± 0.057
0.314TyrTrp: 0.314 ± 0.021
1.653TyrTyr: 1.653 ± 0.053
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2889 proteins (797397 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski