Amorphotheca resinae ATCC 22711

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Leotiomycetes incertae sedis; Myxotrichaceae; Amorphotheca; Amorphotheca resinae

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9640 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T3B8W6|A0A2T3B8W6_AMORE Uncharacterized protein (Fragment) OS=Amorphotheca resinae ATCC 22711 OX=857342 GN=M430DRAFT_117406 PE=4 SV=1
MM1 pKa = 7.74PSSLIYY7 pKa = 10.58SNHH10 pKa = 6.74DD11 pKa = 3.32PSPSVTCPICLDD23 pKa = 3.72PVTAEE28 pKa = 4.06AVRR31 pKa = 11.84LSCHH35 pKa = 6.06LQLHH39 pKa = 6.32CSEE42 pKa = 5.55CIGYY46 pKa = 8.88WLGSLVAAAKK56 pKa = 10.1RR57 pKa = 11.84VTCPKK62 pKa = 9.78CRR64 pKa = 11.84EE65 pKa = 4.03AVTVEE70 pKa = 3.87NLRR73 pKa = 11.84APEE76 pKa = 4.41QNVEE80 pKa = 3.91WQDD83 pKa = 3.56PDD85 pKa = 3.54GDD87 pKa = 4.13EE88 pKa = 4.21YY89 pKa = 11.43DD90 pKa = 4.02YY91 pKa = 11.26DD92 pKa = 4.4YY93 pKa = 11.75DD94 pKa = 6.33DD95 pKa = 5.19YY96 pKa = 12.04DD97 pKa = 5.99DD98 pKa = 5.63YY99 pKa = 12.06DD100 pKa = 3.67DD101 pKa = 4.66TEE103 pKa = 4.44VEE105 pKa = 4.12EE106 pKa = 4.29EE107 pKa = 4.15NRR109 pKa = 11.84DD110 pKa = 3.68SEE112 pKa = 4.24EE113 pKa = 4.39DD114 pKa = 3.43QDD116 pKa = 3.71EE117 pKa = 4.67DD118 pKa = 5.23GYY120 pKa = 11.23QSSDD124 pKa = 3.14IEE126 pKa = 5.69DD127 pKa = 3.58GLNYY131 pKa = 10.53YY132 pKa = 10.5GADD135 pKa = 3.28QDD137 pKa = 4.14EE138 pKa = 4.58NEE140 pKa = 4.5DD141 pKa = 4.31LISGEE146 pKa = 4.05MGEE149 pKa = 5.85DD150 pKa = 2.78IDD152 pKa = 5.97AYY154 pKa = 10.46EE155 pKa = 4.36EE156 pKa = 4.37DD157 pKa = 3.41EE158 pKa = 4.54GGIFEE163 pKa = 5.24RR164 pKa = 11.84DD165 pKa = 3.21PDD167 pKa = 3.79EE168 pKa = 7.27DD169 pKa = 4.12EE170 pKa = 5.65DD171 pKa = 4.68QMSDD175 pKa = 3.19EE176 pKa = 4.38MKK178 pKa = 9.62GTEE181 pKa = 4.05SEE183 pKa = 4.31EE184 pKa = 4.17PKK186 pKa = 10.59EE187 pKa = 3.97GLQVTVRR194 pKa = 11.84EE195 pKa = 4.15EE196 pKa = 4.03PPNKK200 pKa = 9.32KK201 pKa = 10.02RR202 pKa = 11.84SISPPIGVSIPRR214 pKa = 11.84QNSQTMKK221 pKa = 10.83DD222 pKa = 3.67GAQQ225 pKa = 3.05

Molecular weight: 25.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T3BFI4|A0A2T3BFI4_AMORE Amidase domain-containing protein OS=Amorphotheca resinae ATCC 22711 OX=857342 GN=M430DRAFT_24495 PE=4 SV=1
MM1 pKa = 6.93NTLATNPPSLFTFRR15 pKa = 11.84PPAPPPLRR23 pKa = 11.84QSVSQSLRR31 pKa = 11.84PSSPTMRR38 pKa = 11.84SSRR41 pKa = 11.84SSLINRR47 pKa = 11.84PPSLPPPP54 pKa = 3.99

Molecular weight: 5.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9640

0

9640

4384405

49

4859

454.8

50.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.366 ± 0.022

1.172 ± 0.009

5.543 ± 0.018

6.454 ± 0.027

3.651 ± 0.018

6.944 ± 0.023

2.282 ± 0.009

5.055 ± 0.02

5.123 ± 0.022

8.829 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.163 ± 0.009

3.689 ± 0.015

6.264 ± 0.027

3.914 ± 0.02

6.175 ± 0.024

8.443 ± 0.03

5.871 ± 0.016

5.892 ± 0.018

1.396 ± 0.009

2.774 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski