Acyrthosiphon pisum (Pea aphid)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Paraneoptera; Hemiptera; Sternorrhyncha; Aphidomorpha; Aphidoidea; Aphididae;

Average proteome isoelectric point is 7.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35819 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J9JKK1|J9JKK1_ACYPI Uncharacterized protein OS=Acyrthosiphon pisum OX=7029 GN=100159313 PE=3 SV=2
MM1 pKa = 7.63DD2 pKa = 4.36TLASFNEE9 pKa = 4.01EE10 pKa = 4.17DD11 pKa = 4.74EE12 pKa = 5.26SIQHH16 pKa = 6.3CEE18 pKa = 3.83PNVTFIVDD26 pKa = 3.27EE27 pKa = 4.22ATFGKK32 pKa = 8.7GTLYY36 pKa = 10.56VAEE39 pKa = 4.74SKK41 pKa = 10.95LYY43 pKa = 9.1WKK45 pKa = 10.61NDD47 pKa = 2.88ATNQIISIDD56 pKa = 3.97YY57 pKa = 10.77KK58 pKa = 10.47SMCVFGTCNHH68 pKa = 6.38PVVHH72 pKa = 6.82EE73 pKa = 4.57KK74 pKa = 10.57PCLQIIVDD82 pKa = 4.24FSYY85 pKa = 10.86KK86 pKa = 10.16PSDD89 pKa = 3.76SIQPEE94 pKa = 3.7NGQNLNGDD102 pKa = 3.39NHH104 pKa = 7.56INEE107 pKa = 5.26DD108 pKa = 4.32DD109 pKa = 4.33NDD111 pKa = 4.32SEE113 pKa = 5.37DD114 pKa = 4.49DD115 pKa = 4.01NEE117 pKa = 3.98VDD119 pKa = 3.53EE120 pKa = 5.96NEE122 pKa = 4.27DD123 pKa = 3.62EE124 pKa = 4.76EE125 pKa = 4.35EE126 pKa = 5.72GEE128 pKa = 4.23MKK130 pKa = 10.64SKK132 pKa = 10.58IKK134 pKa = 10.43LVPDD138 pKa = 3.32TPEE141 pKa = 3.88YY142 pKa = 11.13LNEE145 pKa = 3.95IYY147 pKa = 10.81LAFTRR152 pKa = 11.84VQLLHH157 pKa = 6.1NTNDD161 pKa = 3.36SDD163 pKa = 4.31TEE165 pKa = 4.18EE166 pKa = 4.46EE167 pKa = 4.55DD168 pKa = 3.17GDD170 pKa = 4.27YY171 pKa = 11.47YY172 pKa = 11.61NEE174 pKa = 3.8NDD176 pKa = 3.38EE177 pKa = 4.84FEE179 pKa = 6.12DD180 pKa = 3.82YY181 pKa = 11.24DD182 pKa = 4.61EE183 pKa = 5.71YY184 pKa = 11.69DD185 pKa = 4.27DD186 pKa = 4.96EE187 pKa = 4.57QLLRR191 pKa = 11.84NN192 pKa = 3.99

Molecular weight: 22.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|X1X430|X1X430_ACYPI DNA-directed DNA polymerase OS=Acyrthosiphon pisum OX=7029 PE=4 SV=1
QQ1 pKa = 7.27LWRR4 pKa = 11.84TLLQPTRR11 pKa = 11.84THH13 pKa = 5.29TRR15 pKa = 11.84LTHH18 pKa = 6.01RR19 pKa = 11.84LTRR22 pKa = 11.84TRR24 pKa = 11.84TRR26 pKa = 11.84TQLHH30 pKa = 5.49TRR32 pKa = 11.84PLTPLTRR39 pKa = 11.84ATLTLTMTASTGQANTRR56 pKa = 11.84QRR58 pKa = 11.84HH59 pKa = 4.32TTQLTSPPTPRR70 pKa = 11.84FTTTEE75 pKa = 3.79HH76 pKa = 6.34TLPTTLSKK84 pKa = 11.12SSGNTRR90 pKa = 11.84SFF92 pKa = 3.22

Molecular weight: 10.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

35816

3

35819

13890312

19

20627

387.8

43.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.353 ± 0.02

2.165 ± 0.018

5.397 ± 0.01

5.872 ± 0.018

4.102 ± 0.011

4.809 ± 0.015

2.546 ± 0.008

6.708 ± 0.015

7.188 ± 0.022

9.072 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.268 ± 0.008

6.134 ± 0.016

4.617 ± 0.017

3.894 ± 0.013

5.112 ± 0.017

8.069 ± 0.016

6.082 ± 0.015

6.026 ± 0.011

1.138 ± 0.005

3.384 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski