Amino acid dipepetide frequency for Acyrthosiphon pisum (Pea aphid)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.624AlaAla: 4.624 ± 0.051	1.059AlaCys: 1.059 ± 0.015	2.856AlaAsp: 2.856 ± 0.015	3.053AlaGlu: 3.053 ± 0.018	2.108AlaPhe: 2.108 ± 0.014	2.72AlaGly: 2.72 ± 0.031	1.263AlaHis: 1.263 ± 0.012	3.477AlaIle: 3.477 ± 0.027	3.377AlaLys: 3.377 ± 0.02	4.932AlaLeu: 4.932 ± 0.034	1.282AlaMet: 1.282 ± 0.012	2.51AlaAsn: 2.51 ± 0.014	2.34AlaPro: 2.34 ± 0.027	1.767AlaGln: 1.767 ± 0.013	2.755AlaArg: 2.755 ± 0.028	3.945AlaSer: 3.945 ± 0.022	3.342AlaThr: 3.342 ± 0.025	3.878AlaVal: 3.878 ± 0.03	0.571AlaTrp: 0.571 ± 0.006	1.668AlaTyr: 1.668 ± 0.017	0.002AlaXaa: 0.002 ± 0.0
Cys	1.033CysAla: 1.033 ± 0.012	0.551CysCys: 0.551 ± 0.008	1.386CysAsp: 1.386 ± 0.023	1.146CysGlu: 1.146 ± 0.012	0.941CysPhe: 0.941 ± 0.009	1.295CysGly: 1.295 ± 0.023	0.538CysHis: 0.538 ± 0.008	1.311CysIle: 1.311 ± 0.017	1.39CysLys: 1.39 ± 0.016	2.021CysLeu: 2.021 ± 0.024	0.451CysMet: 0.451 ± 0.006	1.234CysAsn: 1.234 ± 0.015	1.069CysPro: 1.069 ± 0.022	0.786CysGln: 0.786 ± 0.013	1.154CysArg: 1.154 ± 0.019	1.843CysSer: 1.843 ± 0.027	1.25CysThr: 1.25 ± 0.015	1.312CysVal: 1.312 ± 0.017	0.235CysTrp: 0.235 ± 0.004	0.701CysTyr: 0.701 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	2.635AspAla: 2.635 ± 0.024	1.119AspCys: 1.119 ± 0.014	3.964AspAsp: 3.964 ± 0.03	3.923AspGlu: 3.923 ± 0.026	2.329AspPhe: 2.329 ± 0.022	2.969AspGly: 2.969 ± 0.021	1.292AspHis: 1.292 ± 0.01	4.047AspIle: 4.047 ± 0.024	3.42AspLys: 3.42 ± 0.022	4.705AspLeu: 4.705 ± 0.022	1.194AspMet: 1.194 ± 0.011	3.247AspAsn: 3.247 ± 0.028	2.081AspPro: 2.081 ± 0.02	1.808AspGln: 1.808 ± 0.012	2.405AspArg: 2.405 ± 0.02	3.984AspSer: 3.984 ± 0.023	2.924AspThr: 2.924 ± 0.02	3.468AspVal: 3.468 ± 0.023	0.597AspTrp: 0.597 ± 0.008	1.857AspTyr: 1.857 ± 0.013	0.001AspXaa: 0.001 ± 0.0
Glu	2.932GluAla: 2.932 ± 0.019	1.193GluCys: 1.193 ± 0.022	3.355GluAsp: 3.355 ± 0.018	4.49GluGlu: 4.49 ± 0.04	2.309GluPhe: 2.309 ± 0.013	2.102GluGly: 2.102 ± 0.014	1.406GluHis: 1.406 ± 0.012	4.271GluIle: 4.271 ± 0.028	4.782GluLys: 4.782 ± 0.031	5.325GluLeu: 5.325 ± 0.026	1.429GluMet: 1.429 ± 0.011	4.267GluAsn: 4.267 ± 0.028	2.066GluPro: 2.066 ± 0.017	2.284GluGln: 2.284 ± 0.018	2.838GluArg: 2.838 ± 0.021	4.073GluSer: 4.073 ± 0.022	3.561GluThr: 3.561 ± 0.026	3.345GluVal: 3.345 ± 0.02	0.683GluTrp: 0.683 ± 0.008	2.005GluTyr: 2.005 ± 0.013	0.001GluXaa: 0.001 ± 0.0
Phe	1.915PheAla: 1.915 ± 0.013	0.905PheCys: 0.905 ± 0.009	2.34PheAsp: 2.34 ± 0.013	2.4PheGlu: 2.4 ± 0.014	1.717PhePhe: 1.717 ± 0.014	2.285PheGly: 2.285 ± 0.017	0.967PheHis: 0.967 ± 0.008	2.796PheIle: 2.796 ± 0.025	3.0PheLys: 3.0 ± 0.019	3.753PheLeu: 3.753 ± 0.02	0.907PheMet: 0.907 ± 0.008	2.576PheAsn: 2.576 ± 0.019	1.76PhePro: 1.76 ± 0.024	1.494PheGln: 1.494 ± 0.011	1.93PheArg: 1.93 ± 0.014	3.512PheSer: 3.512 ± 0.02	2.265PheThr: 2.265 ± 0.014	2.575PheVal: 2.575 ± 0.015	0.478PheTrp: 0.478 ± 0.007	1.44PheTyr: 1.44 ± 0.013	0.001PheXaa: 0.001 ± 0.0
Gly	2.563GlyAla: 2.563 ± 0.019	1.149GlyCys: 1.149 ± 0.025	2.465GlyAsp: 2.465 ± 0.017	2.461GlyGlu: 2.461 ± 0.02	2.088GlyPhe: 2.088 ± 0.015	3.46GlyGly: 3.46 ± 0.044	1.545GlyHis: 1.545 ± 0.026	3.119GlyIle: 3.119 ± 0.017	3.1GlyLys: 3.1 ± 0.019	4.145GlyLeu: 4.145 ± 0.019	0.998GlyMet: 0.998 ± 0.01	2.466GlyAsn: 2.466 ± 0.018	2.096GlyPro: 2.096 ± 0.024	1.717GlyGln: 1.717 ± 0.013	2.715GlyArg: 2.715 ± 0.031	3.843GlySer: 3.843 ± 0.027	2.762GlyThr: 2.762 ± 0.018	3.1GlyVal: 3.1 ± 0.026	0.572GlyTrp: 0.572 ± 0.007	1.721GlyTyr: 1.721 ± 0.017	0.002GlyXaa: 0.002 ± 0.0
His	1.085HisAla: 1.085 ± 0.023	0.579HisCys: 0.579 ± 0.006	1.11HisAsp: 1.11 ± 0.01	1.221HisGlu: 1.221 ± 0.012	1.241HisPhe: 1.241 ± 0.011	1.307HisGly: 1.307 ± 0.025	1.001HisHis: 1.001 ± 0.017	1.761HisIle: 1.761 ± 0.024	1.622HisLys: 1.622 ± 0.013	2.519HisLeu: 2.519 ± 0.017	0.585HisMet: 0.585 ± 0.007	1.429HisAsn: 1.429 ± 0.01	1.192HisPro: 1.192 ± 0.01	1.144HisGln: 1.144 ± 0.014	1.512HisArg: 1.512 ± 0.015	1.981HisSer: 1.981 ± 0.015	1.565HisThr: 1.565 ± 0.02	1.415HisVal: 1.415 ± 0.011	0.301HisTrp: 0.301 ± 0.004	0.887HisTyr: 0.887 ± 0.009	0.001HisXaa: 0.001 ± 0.0
Ile	3.31IleAla: 3.31 ± 0.017	1.487IleCys: 1.487 ± 0.026	3.897IleAsp: 3.897 ± 0.022	4.16IleGlu: 4.16 ± 0.024	2.785IlePhe: 2.785 ± 0.028	2.996IleGly: 2.996 ± 0.017	1.616IleHis: 1.616 ± 0.011	4.767IleIle: 4.767 ± 0.027	5.21IleLys: 5.21 ± 0.032	6.078IleLeu: 6.078 ± 0.031	1.479IleMet: 1.479 ± 0.013	4.42IleAsn: 4.42 ± 0.025	3.141IlePro: 3.141 ± 0.02	2.676IleGln: 2.676 ± 0.018	3.116IleArg: 3.116 ± 0.019	5.24IleSer: 5.24 ± 0.025	3.925IleThr: 3.925 ± 0.021	3.975IleVal: 3.975 ± 0.021	0.655IleTrp: 0.655 ± 0.021	2.146IleTyr: 2.146 ± 0.015	0.002IleXaa: 0.002 ± 0.0
Lys	3.143LysAla: 3.143 ± 0.02	1.711LysCys: 1.711 ± 0.019	3.307LysAsp: 3.307 ± 0.02	4.142LysGlu: 4.142 ± 0.027	2.663LysPhe: 2.663 ± 0.015	2.546LysGly: 2.546 ± 0.019	1.864LysHis: 1.864 ± 0.016	5.202LysIle: 5.202 ± 0.03	6.385LysLys: 6.385 ± 0.042	6.8LysLeu: 6.8 ± 0.035	1.78LysMet: 1.78 ± 0.014	4.952LysAsn: 4.952 ± 0.031	3.189LysPro: 3.189 ± 0.028	2.738LysGln: 2.738 ± 0.016	4.021LysArg: 4.021 ± 0.026	5.702LysSer: 5.702 ± 0.031	4.497LysThr: 4.497 ± 0.026	3.625LysVal: 3.625 ± 0.02	0.867LysTrp: 0.867 ± 0.009	2.749LysTyr: 2.749 ± 0.017	0.002LysXaa: 0.002 ± 0.0
Leu	4.941LeuAla: 4.941 ± 0.034	1.903LeuCys: 1.903 ± 0.013	4.688LeuAsp: 4.688 ± 0.023	5.303LeuGlu: 5.303 ± 0.029	3.591LeuPhe: 3.591 ± 0.02	3.878LeuGly: 3.878 ± 0.021	2.284LeuHis: 2.284 ± 0.025	5.439LeuIle: 5.439 ± 0.023	7.429LeuLys: 7.429 ± 0.034	8.596LeuLeu: 8.596 ± 0.043	2.153LeuMet: 2.153 ± 0.014	5.714LeuAsn: 5.714 ± 0.028	4.488LeuPro: 4.488 ± 0.025	4.002LeuGln: 4.002 ± 0.027	4.702LeuArg: 4.702 ± 0.029	7.45LeuSer: 7.45 ± 0.03	5.281LeuThr: 5.281 ± 0.023	4.98LeuVal: 4.98 ± 0.025	1.005LeuTrp: 1.005 ± 0.009	2.892LeuTyr: 2.892 ± 0.018	0.003LeuXaa: 0.003 ± 0.001
Met	1.503MetAla: 1.503 ± 0.012	0.524MetCys: 0.524 ± 0.006	1.351MetAsp: 1.351 ± 0.012	1.359MetGlu: 1.359 ± 0.011	1.026MetPhe: 1.026 ± 0.009	0.966MetGly: 0.966 ± 0.009	0.513MetHis: 0.513 ± 0.007	1.322MetIle: 1.322 ± 0.011	1.611MetLys: 1.611 ± 0.013	1.971MetLeu: 1.971 ± 0.014	0.607MetMet: 0.607 ± 0.008	1.285MetAsn: 1.285 ± 0.011	0.991MetPro: 0.991 ± 0.01	0.781MetGln: 0.781 ± 0.008	1.016MetArg: 1.016 ± 0.009	1.993MetSer: 1.993 ± 0.014	1.363MetThr: 1.363 ± 0.011	1.399MetVal: 1.399 ± 0.01	0.254MetTrp: 0.254 ± 0.005	0.846MetTyr: 0.846 ± 0.009	0.001MetXaa: 0.001 ± 0.0
Asn	2.889AsnAla: 2.889 ± 0.018	1.325AsnCys: 1.325 ± 0.012	3.435AsnAsp: 3.435 ± 0.023	3.839AsnGlu: 3.839 ± 0.022	2.465AsnPhe: 2.465 ± 0.022	3.065AsnGly: 3.065 ± 0.025	1.461AsnHis: 1.461 ± 0.014	4.966AsnIle: 4.966 ± 0.031	4.386AsnLys: 4.386 ± 0.025	5.215AsnLeu: 5.215 ± 0.026	1.399AsnMet: 1.399 ± 0.01	4.688AsnAsn: 4.688 ± 0.032	2.298AsnPro: 2.298 ± 0.026	2.166AsnGln: 2.166 ± 0.016	2.721AsnArg: 2.721 ± 0.016	4.782AsnSer: 4.782 ± 0.026	3.585AsnThr: 3.585 ± 0.023	3.748AsnVal: 3.748 ± 0.019	0.666AsnTrp: 0.666 ± 0.008	2.238AsnTyr: 2.238 ± 0.015	0.002AsnXaa: 0.002 ± 0.0
Pro	2.621ProAla: 2.621 ± 0.031	0.813ProCys: 0.813 ± 0.029	2.311ProAsp: 2.311 ± 0.026	2.687ProGlu: 2.687 ± 0.02	1.667ProPhe: 1.667 ± 0.014	2.319ProGly: 2.319 ± 0.039	1.047ProHis: 1.047 ± 0.011	2.976ProIle: 2.976 ± 0.017	2.947ProLys: 2.947 ± 0.019	3.93ProLeu: 3.93 ± 0.02	0.92ProMet: 0.92 ± 0.01	2.552ProAsn: 2.552 ± 0.017	3.227ProPro: 3.227 ± 0.038	1.799ProGln: 1.799 ± 0.015	2.288ProArg: 2.288 ± 0.029	3.975ProSer: 3.975 ± 0.024	2.988ProThr: 2.988 ± 0.02	3.051ProVal: 3.051 ± 0.02	0.501ProTrp: 0.501 ± 0.011	1.554ProTyr: 1.554 ± 0.016	0.002ProXaa: 0.002 ± 0.0
Gln	1.835GlnAla: 1.835 ± 0.015	0.812GlnCys: 0.812 ± 0.014	1.62GlnAsp: 1.62 ± 0.012	2.013GlnGlu: 2.013 ± 0.016	1.618GlnPhe: 1.618 ± 0.012	1.504GlnGly: 1.504 ± 0.013	1.166GlnHis: 1.166 ± 0.013	2.58GlnIle: 2.58 ± 0.014	2.656GlnLys: 2.656 ± 0.015	3.807GlnLeu: 3.807 ± 0.023	0.946GlnMet: 0.946 ± 0.008	2.559GlnAsn: 2.559 ± 0.021	1.893GlnPro: 1.893 ± 0.021	2.815GlnGln: 2.815 ± 0.061	2.032GlnArg: 2.032 ± 0.016	2.991GlnSer: 2.991 ± 0.019	2.255GlnThr: 2.255 ± 0.015	2.063GlnVal: 2.063 ± 0.023	0.475GlnTrp: 0.475 ± 0.006	1.298GlnTyr: 1.298 ± 0.01	0.001GlnXaa: 0.001 ± 0.0
Arg	2.687ArgAla: 2.687 ± 0.067	1.112ArgCys: 1.112 ± 0.013	2.269ArgAsp: 2.269 ± 0.017	2.544ArgGlu: 2.544 ± 0.018	2.086ArgPhe: 2.086 ± 0.013	2.428ArgGly: 2.428 ± 0.031	1.467ArgHis: 1.467 ± 0.013	3.004ArgIle: 3.004 ± 0.016	3.75ArgLys: 3.75 ± 0.022	4.914ArgLeu: 4.914 ± 0.029	1.103ArgMet: 1.103 ± 0.009	2.8ArgAsn: 2.8 ± 0.016	2.627ArgPro: 2.627 ± 0.022	2.089ArgGln: 2.089 ± 0.016	4.037ArgArg: 4.037 ± 0.036	3.976ArgSer: 3.976 ± 0.023	2.984ArgThr: 2.984 ± 0.024	2.825ArgVal: 2.825 ± 0.017	0.763ArgTrp: 0.763 ± 0.009	1.655ArgTyr: 1.655 ± 0.011	0.002ArgXaa: 0.002 ± 0.0
Ser	4.3SerAla: 4.3 ± 0.024	1.575SerCys: 1.575 ± 0.022	4.412SerAsp: 4.412 ± 0.026	4.492SerGlu: 4.492 ± 0.023	3.216SerPhe: 3.216 ± 0.02	4.023SerGly: 4.023 ± 0.024	1.839SerHis: 1.839 ± 0.025	5.278SerIle: 5.278 ± 0.027	5.456SerLys: 5.456 ± 0.03	6.904SerLeu: 6.904 ± 0.028	1.741SerMet: 1.741 ± 0.012	4.918SerAsn: 4.918 ± 0.027	3.837SerPro: 3.837 ± 0.028	2.799SerGln: 2.799 ± 0.017	3.845SerArg: 3.845 ± 0.022	8.222SerSer: 8.222 ± 0.046	5.546SerThr: 5.546 ± 0.029	4.896SerVal: 4.896 ± 0.024	0.819SerTrp: 0.819 ± 0.008	2.572SerTyr: 2.572 ± 0.02	0.003SerXaa: 0.003 ± 0.0
Thr	3.638ThrAla: 3.638 ± 0.023	1.294ThrCys: 1.294 ± 0.017	3.23ThrAsp: 3.23 ± 0.02	3.466ThrGlu: 3.466 ± 0.025	2.49ThrPhe: 2.49 ± 0.015	3.084ThrGly: 3.084 ± 0.021	1.455ThrHis: 1.455 ± 0.018	4.13ThrIle: 4.13 ± 0.02	3.975ThrLys: 3.975 ± 0.021	5.279ThrLeu: 5.279 ± 0.023	1.324ThrMet: 1.324 ± 0.01	3.592ThrAsn: 3.592 ± 0.02	3.014ThrPro: 3.014 ± 0.017	2.016ThrGln: 2.016 ± 0.023	2.705ThrArg: 2.705 ± 0.018	5.198ThrSer: 5.198 ± 0.023	4.416ThrThr: 4.416 ± 0.034	3.966ThrVal: 3.966 ± 0.021	0.658ThrTrp: 0.658 ± 0.008	1.893ThrTyr: 1.893 ± 0.012	0.001ThrXaa: 0.001 ± 0.0
Val	3.526ValAla: 3.526 ± 0.021	1.46ValCys: 1.46 ± 0.021	3.508ValAsp: 3.508 ± 0.024	3.637ValGlu: 3.637 ± 0.025	2.59ValPhe: 2.59 ± 0.016	2.844ValGly: 2.844 ± 0.02	1.55ValHis: 1.55 ± 0.011	3.773ValIle: 3.773 ± 0.02	4.057ValLys: 4.057 ± 0.021	5.58ValLeu: 5.58 ± 0.025	1.349ValMet: 1.349 ± 0.012	3.265ValAsn: 3.265 ± 0.018	2.915ValPro: 2.915 ± 0.027	2.377ValGln: 2.377 ± 0.016	2.88ValArg: 2.88 ± 0.018	4.5ValSer: 4.5 ± 0.023	3.691ValThr: 3.691 ± 0.02	4.106ValVal: 4.106 ± 0.03	0.681ValTrp: 0.681 ± 0.008	1.969ValTyr: 1.969 ± 0.014	0.002ValXaa: 0.002 ± 0.0
Trp	0.573TrpAla: 0.573 ± 0.007	0.245TrpCys: 0.245 ± 0.004	0.584TrpAsp: 0.584 ± 0.008	0.536TrpGlu: 0.536 ± 0.007	0.476TrpPhe: 0.476 ± 0.007	0.489TrpGly: 0.489 ± 0.007	0.286TrpHis: 0.286 ± 0.005	0.689TrpIle: 0.689 ± 0.007	0.958TrpLys: 0.958 ± 0.009	1.081TrpLeu: 1.081 ± 0.009	0.275TrpMet: 0.275 ± 0.004	0.738TrpAsn: 0.738 ± 0.022	0.515TrpPro: 0.515 ± 0.006	0.437TrpGln: 0.437 ± 0.006	0.69TrpArg: 0.69 ± 0.008	0.935TrpSer: 0.935 ± 0.009	0.747TrpThr: 0.747 ± 0.009	0.569TrpVal: 0.569 ± 0.007	0.18TrpTrp: 0.18 ± 0.004	0.371TrpTyr: 0.371 ± 0.006	0.001TrpXaa: 0.001 ± 0.0
Tyr	1.776TyrAla: 1.776 ± 0.018	0.827TyrCys: 0.827 ± 0.01	1.881TyrAsp: 1.881 ± 0.015	1.845TyrGlu: 1.845 ± 0.013	1.608TyrPhe: 1.608 ± 0.013	1.805TyrGly: 1.805 ± 0.015	0.895TyrHis: 0.895 ± 0.009	2.168TyrIle: 2.168 ± 0.013	2.372TyrLys: 2.372 ± 0.017	3.165TyrLeu: 3.165 ± 0.018	0.76TyrMet: 0.76 ± 0.007	2.123TyrAsn: 2.123 ± 0.016	1.434TyrPro: 1.434 ± 0.017	1.239TyrGln: 1.239 ± 0.01	1.761TyrArg: 1.761 ± 0.014	2.546TyrSer: 2.546 ± 0.02	1.877TyrThr: 1.877 ± 0.014	1.964TyrVal: 1.964 ± 0.014	0.415TyrTrp: 0.415 ± 0.006	1.374TyrTyr: 1.374 ± 0.013	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.0	0.001XaaGlu: 0.001 ± 0.0	0.003XaaPhe: 0.003 ± 0.0	0.001XaaGly: 0.001 ± 0.0	0.001XaaHis: 0.001 ± 0.0	0.004XaaIle: 0.004 ± 0.001	0.002XaaLys: 0.002 ± 0.0	0.003XaaLeu: 0.003 ± 0.001	0.001XaaMet: 0.001 ± 0.0	0.002XaaAsn: 0.002 ± 0.0	0.001XaaPro: 0.001 ± 0.0	0.001XaaGln: 0.001 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.003XaaSer: 0.003 ± 0.0	0.002XaaThr: 0.002 ± 0.0	0.002XaaVal: 0.002 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	0.613XaaXaa: 0.613 ± 0.04

Statistics based on 35819 proteins (13890312 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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