Cytophaga hutchinsonii (strain ATCC 33406 / DSM 1761 / CIP 103989 / NBRC 15051 / NCIMB 9469 / D465)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Cytophaga; Cytophaga hutchinsonii

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3771 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N4SRP6|A0A6N4SRP6_CYTH3 Isochorismate synthase OS=Cytophaga hutchinsonii (strain ATCC 33406 / DSM 1761 / CIP 103989 / NBRC 15051 / NCIMB 9469 / D465) OX=269798 GN=entC PE=4 SV=1
MM1 pKa = 7.9IIQDD5 pKa = 4.37YY6 pKa = 10.19IDD8 pKa = 3.57YY9 pKa = 10.26WNEE12 pKa = 3.2QDD14 pKa = 5.4IEE16 pKa = 4.13LDD18 pKa = 3.76KK19 pKa = 11.28IKK21 pKa = 10.78LEE23 pKa = 4.26SVHH26 pKa = 7.08AIPLPVDD33 pKa = 2.9ILEE36 pKa = 4.28FLSMVGLPKK45 pKa = 10.17EE46 pKa = 3.96AAPYY50 pKa = 8.76LTFNITSFPAFVSPDD65 pKa = 3.55DD66 pKa = 5.44LYY68 pKa = 11.63DD69 pKa = 4.64LEE71 pKa = 5.31DD72 pKa = 5.16RR73 pKa = 11.84PDD75 pKa = 3.52LSDD78 pKa = 2.94WFIIGEE84 pKa = 4.43TGNSDD89 pKa = 4.91PICIDD94 pKa = 2.83PMGGYY99 pKa = 9.67RR100 pKa = 11.84ICYY103 pKa = 9.55VEE105 pKa = 4.31TEE107 pKa = 4.24NEE109 pKa = 3.87FNAVFMNSSLDD120 pKa = 3.4KK121 pKa = 11.0LAICIYY127 pKa = 10.0LYY129 pKa = 10.96EE130 pKa = 4.35KK131 pKa = 10.48FIEE134 pKa = 4.36KK135 pKa = 10.39FSTEE139 pKa = 3.65EE140 pKa = 4.03SEE142 pKa = 4.59NLVFNDD148 pKa = 4.86ADD150 pKa = 3.38WEE152 pKa = 4.28YY153 pKa = 11.44LKK155 pKa = 10.89KK156 pKa = 10.52QFMDD160 pKa = 3.61IDD162 pKa = 4.05PEE164 pKa = 4.23ALSEE168 pKa = 4.29GSFWDD173 pKa = 3.91YY174 pKa = 11.01YY175 pKa = 11.39LEE177 pKa = 4.12YY178 pKa = 11.13LLVDD182 pKa = 4.03RR183 pKa = 11.84DD184 pKa = 3.6

Molecular weight: 21.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N4SQ95|A0A6N4SQ95_CYTH3 HTH cro/C1-type domain-containing protein OS=Cytophaga hutchinsonii (strain ATCC 33406 / DSM 1761 / CIP 103989 / NBRC 15051 / NCIMB 9469 / D465) OX=269798 GN=CHU_1178 PE=4 SV=1
MM1 pKa = 8.03RR2 pKa = 11.84IFLTEE7 pKa = 3.89QPCWITATPAGGTTGKK23 pKa = 9.86SLCATTDD30 pKa = 2.21IFMYY34 pKa = 9.43LTFSKK39 pKa = 9.25NTLSIFPQPFTQNVVLSLKK58 pKa = 10.36NNPINPLLFSICRR71 pKa = 11.84VFNRR75 pKa = 11.84IPSRR79 pKa = 11.84YY80 pKa = 7.66RR81 pKa = 11.84HH82 pKa = 5.9LRR84 pKa = 11.84NTIRR88 pKa = 11.84GIFTT92 pKa = 3.62

Molecular weight: 10.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3771

0

3771

1325175

49

5453

351.4

39.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.259 ± 0.053

1.045 ± 0.016

5.069 ± 0.029

5.643 ± 0.058

4.912 ± 0.037

6.433 ± 0.048

1.865 ± 0.025

7.868 ± 0.043

6.981 ± 0.062

9.089 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.236 ± 0.025

5.559 ± 0.042

3.641 ± 0.027

3.459 ± 0.022

3.352 ± 0.032

6.869 ± 0.045

6.739 ± 0.107

6.469 ± 0.033

1.084 ± 0.013

4.428 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski