Amino acid dipepetide frequency for Cytophaga hutchinsonii (strain ATCC 33406 / DSM 1761 / CIP 103989 / NBRC 15051 / NCIMB 9469 / D465)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.652AlaAla: 6.652 ± 0.122
0.886AlaCys: 0.886 ± 0.025
3.998AlaAsp: 3.998 ± 0.061
3.916AlaGlu: 3.916 ± 0.071
3.573AlaPhe: 3.573 ± 0.06
5.752AlaGly: 5.752 ± 0.121
1.322AlaHis: 1.322 ± 0.032
5.161AlaIle: 5.161 ± 0.076
4.089AlaLys: 4.089 ± 0.064
6.673AlaLeu: 6.673 ± 0.086
1.548AlaMet: 1.548 ± 0.039
3.394AlaAsn: 3.394 ± 0.07
2.411AlaPro: 2.411 ± 0.07
2.633AlaGln: 2.633 ± 0.041
2.116AlaArg: 2.116 ± 0.043
5.045AlaSer: 5.045 ± 0.102
4.817AlaThr: 4.817 ± 0.142
4.937AlaVal: 4.937 ± 0.082
0.731AlaTrp: 0.731 ± 0.028
2.935AlaTyr: 2.935 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
0.753CysAla: 0.753 ± 0.034
0.161CysCys: 0.161 ± 0.012
0.474CysAsp: 0.474 ± 0.02
0.487CysGlu: 0.487 ± 0.021
0.517CysPhe: 0.517 ± 0.022
0.787CysGly: 0.787 ± 0.028
0.203CysHis: 0.203 ± 0.015
0.935CysIle: 0.935 ± 0.03
0.655CysLys: 0.655 ± 0.024
0.88CysLeu: 0.88 ± 0.025
0.271CysMet: 0.271 ± 0.014
0.557CysAsn: 0.557 ± 0.027
0.398CysPro: 0.398 ± 0.026
0.248CysGln: 0.248 ± 0.013
0.309CysArg: 0.309 ± 0.016
0.915CysSer: 0.915 ± 0.034
0.73CysThr: 0.73 ± 0.032
0.629CysVal: 0.629 ± 0.024
0.106CysTrp: 0.106 ± 0.009
0.431CysTyr: 0.431 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
3.955AspAla: 3.955 ± 0.064
0.445AspCys: 0.445 ± 0.026
2.139AspAsp: 2.139 ± 0.047
2.931AspGlu: 2.931 ± 0.053
2.789AspPhe: 2.789 ± 0.05
3.461AspGly: 3.461 ± 0.06
0.85AspHis: 0.85 ± 0.027
4.069AspIle: 4.069 ± 0.068
3.649AspLys: 3.649 ± 0.059
4.684AspLeu: 4.684 ± 0.071
1.123AspMet: 1.123 ± 0.03
2.475AspAsn: 2.475 ± 0.046
1.905AspPro: 1.905 ± 0.038
1.624AspGln: 1.624 ± 0.035
1.762AspArg: 1.762 ± 0.039
3.283AspSer: 3.283 ± 0.066
3.158AspThr: 3.158 ± 0.062
3.412AspVal: 3.412 ± 0.053
0.662AspTrp: 0.662 ± 0.022
2.313AspTyr: 2.313 ± 0.043
0.0AspXaa: 0.0 ± 0.0
Glu
3.96GluAla: 3.96 ± 0.077
0.426GluCys: 0.426 ± 0.017
2.671GluAsp: 2.671 ± 0.055
3.907GluGlu: 3.907 ± 0.087
2.421GluPhe: 2.421 ± 0.043
3.132GluGly: 3.132 ± 0.052
1.133GluHis: 1.133 ± 0.03
4.691GluIle: 4.691 ± 0.074
4.998GluLys: 4.998 ± 0.092
5.546GluLeu: 5.546 ± 0.092
1.465GluMet: 1.465 ± 0.036
3.135GluAsn: 3.135 ± 0.048
1.448GluPro: 1.448 ± 0.036
2.297GluGln: 2.297 ± 0.053
2.195GluArg: 2.195 ± 0.04
3.2GluSer: 3.2 ± 0.055
3.215GluThr: 3.215 ± 0.053
3.517GluVal: 3.517 ± 0.058
0.656GluTrp: 0.656 ± 0.023
2.415GluTyr: 2.415 ± 0.053
0.0GluXaa: 0.0 ± 0.0
Phe
2.988PheAla: 2.988 ± 0.06
0.596PheCys: 0.596 ± 0.026
2.773PheAsp: 2.773 ± 0.047
2.753PheGlu: 2.753 ± 0.051
2.671PhePhe: 2.671 ± 0.063
2.978PheGly: 2.978 ± 0.054
0.856PheHis: 0.856 ± 0.025
4.075PheIle: 4.075 ± 0.072
3.077PheLys: 3.077 ± 0.057
4.493PheLeu: 4.493 ± 0.076
1.075PheMet: 1.075 ± 0.026
2.837PheAsn: 2.837 ± 0.05
1.661PhePro: 1.661 ± 0.035
1.488PheGln: 1.488 ± 0.038
1.586PheArg: 1.586 ± 0.034
3.964PheSer: 3.964 ± 0.056
3.334PheThr: 3.334 ± 0.056
3.076PheVal: 3.076 ± 0.055
0.529PheTrp: 0.529 ± 0.024
2.304PheTyr: 2.304 ± 0.053
0.0PheXaa: 0.0 ± 0.0
Gly
4.478GlyAla: 4.478 ± 0.081
0.816GlyCys: 0.816 ± 0.036
3.019GlyAsp: 3.019 ± 0.053
3.056GlyGlu: 3.056 ± 0.059
3.288GlyPhe: 3.288 ± 0.06
4.504GlyGly: 4.504 ± 0.097
1.016GlyHis: 1.016 ± 0.034
5.5GlyIle: 5.5 ± 0.063
4.636GlyLys: 4.636 ± 0.071
5.505GlyLeu: 5.505 ± 0.075
1.722GlyMet: 1.722 ± 0.039
3.747GlyAsn: 3.747 ± 0.085
1.53GlyPro: 1.53 ± 0.041
1.862GlyGln: 1.862 ± 0.037
1.997GlyArg: 1.997 ± 0.043
4.567GlySer: 4.567 ± 0.106
5.214GlyThr: 5.214 ± 0.163
4.153GlyVal: 4.153 ± 0.064
0.77GlyTrp: 0.77 ± 0.026
2.945GlyTyr: 2.945 ± 0.052
0.0GlyXaa: 0.0 ± 0.0
His
1.464HisAla: 1.464 ± 0.034
0.206HisCys: 0.206 ± 0.013
0.816HisAsp: 0.816 ± 0.028
0.975HisGlu: 0.975 ± 0.031
1.023HisPhe: 1.023 ± 0.028
1.031HisGly: 1.031 ± 0.03
0.429HisHis: 0.429 ± 0.021
1.555HisIle: 1.555 ± 0.039
1.157HisLys: 1.157 ± 0.034
1.739HisLeu: 1.739 ± 0.042
0.417HisMet: 0.417 ± 0.015
0.862HisAsn: 0.862 ± 0.023
0.894HisPro: 0.894 ± 0.027
0.617HisGln: 0.617 ± 0.021
0.66HisArg: 0.66 ± 0.024
1.196HisSer: 1.196 ± 0.03
1.352HisThr: 1.352 ± 0.037
1.231HisVal: 1.231 ± 0.038
0.197HisTrp: 0.197 ± 0.012
0.835HisTyr: 0.835 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
5.411IleAla: 5.411 ± 0.087
1.032IleCys: 1.032 ± 0.035
4.578IleAsp: 4.578 ± 0.062
4.91IleGlu: 4.91 ± 0.07
3.405IlePhe: 3.405 ± 0.066
4.912IleGly: 4.912 ± 0.076
1.748IleHis: 1.748 ± 0.038
5.69IleIle: 5.69 ± 0.087
5.391IleLys: 5.391 ± 0.073
6.878IleLeu: 6.878 ± 0.09
1.346IleMet: 1.346 ± 0.035
4.183IleAsn: 4.183 ± 0.059
3.445IlePro: 3.445 ± 0.06
3.057IleGln: 3.057 ± 0.049
2.969IleArg: 2.969 ± 0.052
5.675IleSer: 5.675 ± 0.068
5.446IleThr: 5.446 ± 0.102
4.802IleVal: 4.802 ± 0.077
0.717IleTrp: 0.717 ± 0.023
3.088IleTyr: 3.088 ± 0.058
0.0IleXaa: 0.0 ± 0.0
Lys
4.737LysAla: 4.737 ± 0.075
0.387LysCys: 0.387 ± 0.019
3.909LysAsp: 3.909 ± 0.07
5.218LysGlu: 5.218 ± 0.087
2.284LysPhe: 2.284 ± 0.047
4.152LysGly: 4.152 ± 0.066
1.433LysHis: 1.433 ± 0.037
5.543LysIle: 5.543 ± 0.079
6.205LysLys: 6.205 ± 0.111
5.603LysLeu: 5.603 ± 0.08
1.95LysMet: 1.95 ± 0.045
4.595LysAsn: 4.595 ± 0.068
2.528LysPro: 2.528 ± 0.05
2.696LysGln: 2.696 ± 0.055
2.613LysArg: 2.613 ± 0.053
3.996LysSer: 3.996 ± 0.053
4.384LysThr: 4.384 ± 0.068
3.974LysVal: 3.974 ± 0.067
0.739LysTrp: 0.739 ± 0.026
2.868LysTyr: 2.868 ± 0.05
0.0LysXaa: 0.0 ± 0.0
Leu
5.944LeuAla: 5.944 ± 0.077
0.964LeuCys: 0.964 ± 0.027
4.251LeuAsp: 4.251 ± 0.064
4.722LeuGlu: 4.722 ± 0.073
5.008LeuPhe: 5.008 ± 0.081
5.043LeuGly: 5.043 ± 0.073
1.813LeuHis: 1.813 ± 0.042
6.942LeuIle: 6.942 ± 0.102
6.832LeuLys: 6.832 ± 0.089
9.242LeuLeu: 9.242 ± 0.115
1.973LeuMet: 1.973 ± 0.043
5.2LeuAsn: 5.2 ± 0.06
3.817LeuPro: 3.817 ± 0.062
3.316LeuGln: 3.316 ± 0.062
3.056LeuArg: 3.056 ± 0.052
6.995LeuSer: 6.995 ± 0.083
5.973LeuThr: 5.973 ± 0.105
5.282LeuVal: 5.282 ± 0.083
0.857LeuTrp: 0.857 ± 0.027
3.663LeuTyr: 3.663 ± 0.068
0.0LeuXaa: 0.0 ± 0.0
Met
1.453MetAla: 1.453 ± 0.038
0.211MetCys: 0.211 ± 0.012
1.142MetAsp: 1.142 ± 0.032
1.265MetGlu: 1.265 ± 0.037
0.933MetPhe: 0.933 ± 0.031
1.342MetGly: 1.342 ± 0.034
0.556MetHis: 0.556 ± 0.023
1.636MetIle: 1.636 ± 0.04
2.012MetLys: 2.012 ± 0.039
2.17MetLeu: 2.17 ± 0.045
0.535MetMet: 0.535 ± 0.022
1.385MetAsn: 1.385 ± 0.035
0.975MetPro: 0.975 ± 0.027
0.881MetGln: 0.881 ± 0.028
0.912MetArg: 0.912 ± 0.024
1.392MetSer: 1.392 ± 0.034
1.141MetThr: 1.141 ± 0.034
1.291MetVal: 1.291 ± 0.034
0.158MetTrp: 0.158 ± 0.01
0.974MetTyr: 0.974 ± 0.029
0.0MetXaa: 0.0 ± 0.0
Asn
4.046AsnAla: 4.046 ± 0.082
0.485AsnCys: 0.485 ± 0.023
2.609AsnAsp: 2.609 ± 0.059
2.888AsnGlu: 2.888 ± 0.059
2.321AsnPhe: 2.321 ± 0.046
4.027AsnGly: 4.027 ± 0.098
0.911AsnHis: 0.911 ± 0.027
4.491AsnIle: 4.491 ± 0.062
3.95AsnLys: 3.95 ± 0.063
4.419AsnLeu: 4.419 ± 0.077
1.239AsnMet: 1.239 ± 0.029
3.547AsnAsn: 3.547 ± 0.071
2.61AsnPro: 2.61 ± 0.051
2.036AsnGln: 2.036 ± 0.046
1.948AsnArg: 1.948 ± 0.039
3.362AsnSer: 3.362 ± 0.064
4.106AsnThr: 4.106 ± 0.082
3.329AsnVal: 3.329 ± 0.059
0.697AsnTrp: 0.697 ± 0.024
2.566AsnTyr: 2.566 ± 0.05
0.0AsnXaa: 0.0 ± 0.0
Pro
3.521ProAla: 3.521 ± 0.08
0.329ProCys: 0.329 ± 0.017
2.225ProAsp: 2.225 ± 0.044
2.515ProGlu: 2.515 ± 0.052
1.901ProPhe: 1.901 ± 0.038
2.295ProGly: 2.295 ± 0.055
0.638ProHis: 0.638 ± 0.022
2.399ProIle: 2.399 ± 0.048
1.949ProLys: 1.949 ± 0.042
3.071ProLeu: 3.071 ± 0.051
0.715ProMet: 0.715 ± 0.024
1.967ProAsn: 1.967 ± 0.042
0.924ProPro: 0.924 ± 0.037
1.124ProGln: 1.124 ± 0.034
0.868ProArg: 0.868 ± 0.03
2.468ProSer: 2.468 ± 0.056
2.16ProThr: 2.16 ± 0.072
3.368ProVal: 3.368 ± 0.063
0.324ProTrp: 0.324 ± 0.015
1.646ProTyr: 1.646 ± 0.04
0.0ProXaa: 0.0 ± 0.0
Gln
2.585GlnAla: 2.585 ± 0.044
0.257GlnCys: 0.257 ± 0.017
1.447GlnAsp: 1.447 ± 0.039
1.97GlnGlu: 1.97 ± 0.048
1.555GlnPhe: 1.555 ± 0.038
1.805GlnGly: 1.805 ± 0.036
0.777GlnHis: 0.777 ± 0.022
2.545GlnIle: 2.545 ± 0.045
2.534GlnLys: 2.534 ± 0.062
3.565GlnLeu: 3.565 ± 0.058
0.819GlnMet: 0.819 ± 0.024
1.803GlnAsn: 1.803 ± 0.042
1.222GlnPro: 1.222 ± 0.035
1.72GlnGln: 1.72 ± 0.04
1.299GlnArg: 1.299 ± 0.033
2.149GlnSer: 2.149 ± 0.049
2.345GlnThr: 2.345 ± 0.046
2.204GlnVal: 2.204 ± 0.039
0.45GlnTrp: 0.45 ± 0.025
1.538GlnTyr: 1.538 ± 0.036
0.0GlnXaa: 0.0 ± 0.0
Arg
2.028ArgAla: 2.028 ± 0.04
0.281ArgCys: 0.281 ± 0.014
1.616ArgAsp: 1.616 ± 0.041
1.912ArgGlu: 1.912 ± 0.045
1.929ArgPhe: 1.929 ± 0.039
1.677ArgGly: 1.677 ± 0.038
0.555ArgHis: 0.555 ± 0.02
3.143ArgIle: 3.143 ± 0.052
2.616ArgLys: 2.616 ± 0.054
3.274ArgLeu: 3.274 ± 0.061
1.015ArgMet: 1.015 ± 0.027
1.967ArgAsn: 1.967 ± 0.04
1.096ArgPro: 1.096 ± 0.03
1.053ArgGln: 1.053 ± 0.031
1.216ArgArg: 1.216 ± 0.032
2.059ArgSer: 2.059 ± 0.041
1.973ArgThr: 1.973 ± 0.041
2.092ArgVal: 2.092 ± 0.04
0.411ArgTrp: 0.411 ± 0.017
1.604ArgTyr: 1.604 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
5.003SerAla: 5.003 ± 0.103
0.85SerCys: 0.85 ± 0.033
3.464SerAsp: 3.464 ± 0.053
3.36SerGlu: 3.36 ± 0.063
3.756SerPhe: 3.756 ± 0.057
5.443SerGly: 5.443 ± 0.111
1.06SerHis: 1.06 ± 0.031
6.041SerIle: 6.041 ± 0.073
4.193SerLys: 4.193 ± 0.069
6.091SerLeu: 6.091 ± 0.071
1.528SerMet: 1.528 ± 0.034
3.781SerAsn: 3.781 ± 0.077
2.196SerPro: 2.196 ± 0.05
1.859SerGln: 1.859 ± 0.035
2.085SerArg: 2.085 ± 0.042
4.759SerSer: 4.759 ± 0.098
4.511SerThr: 4.511 ± 0.11
4.784SerVal: 4.784 ± 0.073
0.728SerTrp: 0.728 ± 0.026
3.198SerTyr: 3.198 ± 0.059
0.0SerXaa: 0.0 ± 0.0
Thr
5.577ThrAla: 5.577 ± 0.18
0.718ThrCys: 0.718 ± 0.025
3.668ThrAsp: 3.668 ± 0.063
3.359ThrGlu: 3.359 ± 0.051
3.31ThrPhe: 3.31 ± 0.058
5.356ThrGly: 5.356 ± 0.155
1.147ThrHis: 1.147 ± 0.032
5.414ThrIle: 5.414 ± 0.107
3.622ThrLys: 3.622 ± 0.051
5.965ThrLeu: 5.965 ± 0.081
1.067ThrMet: 1.067 ± 0.028
3.434ThrAsn: 3.434 ± 0.072
3.052ThrPro: 3.052 ± 0.091
1.92ThrGln: 1.92 ± 0.042
1.749ThrArg: 1.749 ± 0.035
4.587ThrSer: 4.587 ± 0.109
4.622ThrThr: 4.622 ± 0.159
4.92ThrVal: 4.92 ± 0.137
0.706ThrTrp: 0.706 ± 0.034
3.196ThrTyr: 3.196 ± 0.085
0.0ThrXaa: 0.0 ± 0.0
Val
4.511ValAla: 4.511 ± 0.074
0.842ValCys: 0.842 ± 0.026
3.075ValAsp: 3.075 ± 0.05
3.51ValGlu: 3.51 ± 0.054
3.45ValPhe: 3.45 ± 0.056
3.43ValGly: 3.43 ± 0.065
1.205ValHis: 1.205 ± 0.032
4.858ValIle: 4.858 ± 0.065
4.248ValLys: 4.248 ± 0.065
6.222ValLeu: 6.222 ± 0.069
1.315ValMet: 1.315 ± 0.032
3.455ValAsn: 3.455 ± 0.059
2.413ValPro: 2.413 ± 0.048
2.113ValGln: 2.113 ± 0.044
2.139ValArg: 2.139 ± 0.042
4.998ValSer: 4.998 ± 0.078
4.828ValThr: 4.828 ± 0.14
4.429ValVal: 4.429 ± 0.068
0.735ValTrp: 0.735 ± 0.025
2.918ValTyr: 2.918 ± 0.047
0.0ValXaa: 0.0 ± 0.0
Trp
0.631TrpAla: 0.631 ± 0.027
0.103TrpCys: 0.103 ± 0.009
0.557TrpAsp: 0.557 ± 0.024
0.631TrpGlu: 0.631 ± 0.021
0.555TrpPhe: 0.555 ± 0.025
0.715TrpGly: 0.715 ± 0.033
0.203TrpHis: 0.203 ± 0.013
0.767TrpIle: 0.767 ± 0.024
0.85TrpLys: 0.85 ± 0.031
1.016TrpLeu: 1.016 ± 0.031
0.326TrpMet: 0.326 ± 0.016
0.72TrpAsn: 0.72 ± 0.024
0.257TrpPro: 0.257 ± 0.015
0.443TrpGln: 0.443 ± 0.021
0.337TrpArg: 0.337 ± 0.016
0.744TrpSer: 0.744 ± 0.032
0.694TrpThr: 0.694 ± 0.036
0.642TrpVal: 0.642 ± 0.023
0.123TrpTrp: 0.123 ± 0.011
0.53TrpTyr: 0.53 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.894TyrAla: 2.894 ± 0.055
0.451TyrCys: 0.451 ± 0.022
2.258TyrAsp: 2.258 ± 0.047
2.145TyrGlu: 2.145 ± 0.039
2.428TyrPhe: 2.428 ± 0.049
2.485TyrGly: 2.485 ± 0.041
0.797TyrHis: 0.797 ± 0.025
3.228TyrIle: 3.228 ± 0.049
3.151TyrLys: 3.151 ± 0.057
3.858TyrLeu: 3.858 ± 0.056
0.915TyrMet: 0.915 ± 0.031
2.543TyrAsn: 2.543 ± 0.052
1.624TyrPro: 1.624 ± 0.038
1.601TyrGln: 1.601 ± 0.039
1.698TyrArg: 1.698 ± 0.042
3.337TyrSer: 3.337 ± 0.058
3.384TyrThr: 3.384 ± 0.08
2.622TyrVal: 2.622 ± 0.044
0.549TyrTrp: 0.549 ± 0.021
2.313TyrTyr: 2.313 ± 0.058
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3771 proteins (1325175 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski