Amino acid dipepetide frequency for Epulopiscium sp. SCG-B10WGA-EpuloA2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.442AlaAla: 3.442 ± 0.253
0.605AlaCys: 0.605 ± 0.071
3.212AlaAsp: 3.212 ± 0.155
3.298AlaGlu: 3.298 ± 0.177
2.664AlaPhe: 2.664 ± 0.156
4.205AlaGly: 4.205 ± 0.222
1.066AlaHis: 1.066 ± 0.101
5.912AlaIle: 5.912 ± 0.316
4.652AlaLys: 4.652 ± 0.238
6.618AlaLeu: 6.618 ± 0.232
1.426AlaMet: 1.426 ± 0.098
3.579AlaAsn: 3.579 ± 0.208
2.038AlaPro: 2.038 ± 0.119
2.592AlaGln: 2.592 ± 0.208
1.858AlaArg: 1.858 ± 0.137
3.313AlaSer: 3.313 ± 0.163
4.054AlaThr: 4.054 ± 0.308
3.392AlaVal: 3.392 ± 0.177
0.461AlaTrp: 0.461 ± 0.053
2.117AlaTyr: 2.117 ± 0.147
0.0AlaXaa: 0.0 ± 0.0
Cys
0.619CysAla: 0.619 ± 0.075
0.173CysCys: 0.173 ± 0.038
0.583CysAsp: 0.583 ± 0.07
0.742CysGlu: 0.742 ± 0.085
0.626CysPhe: 0.626 ± 0.075
0.807CysGly: 0.807 ± 0.084
0.209CysHis: 0.209 ± 0.042
0.987CysIle: 0.987 ± 0.095
0.756CysLys: 0.756 ± 0.088
0.771CysLeu: 0.771 ± 0.07
0.252CysMet: 0.252 ± 0.043
0.706CysAsn: 0.706 ± 0.092
0.338CysPro: 0.338 ± 0.064
0.31CysGln: 0.31 ± 0.04
0.382CysArg: 0.382 ± 0.057
0.699CysSer: 0.699 ± 0.075
0.684CysThr: 0.684 ± 0.077
0.886CysVal: 0.886 ± 0.093
0.094CysTrp: 0.094 ± 0.027
0.382CysTyr: 0.382 ± 0.047
0.0CysXaa: 0.0 ± 0.0
Asp
3.413AspAla: 3.413 ± 0.22
0.511AspCys: 0.511 ± 0.07
2.967AspAsp: 2.967 ± 0.216
4.566AspGlu: 4.566 ± 0.214
2.736AspPhe: 2.736 ± 0.161
3.939AspGly: 3.939 ± 0.262
0.648AspHis: 0.648 ± 0.079
6.675AspIle: 6.675 ± 0.217
4.969AspLys: 4.969 ± 0.252
4.486AspLeu: 4.486 ± 0.164
1.656AspMet: 1.656 ± 0.133
3.694AspAsn: 3.694 ± 0.206
1.426AspPro: 1.426 ± 0.118
1.015AspGln: 1.015 ± 0.098
1.613AspArg: 1.613 ± 0.138
2.506AspSer: 2.506 ± 0.141
3.392AspThr: 3.392 ± 0.196
3.759AspVal: 3.759 ± 0.252
0.497AspTrp: 0.497 ± 0.066
2.585AspTyr: 2.585 ± 0.157
0.0AspXaa: 0.0 ± 0.0
Glu
4.076GluAla: 4.076 ± 0.176
0.612GluCys: 0.612 ± 0.073
4.011GluAsp: 4.011 ± 0.231
5.919GluGlu: 5.919 ± 0.291
3.161GluPhe: 3.161 ± 0.163
3.781GluGly: 3.781 ± 0.189
1.318GluHis: 1.318 ± 0.097
7.792GluIle: 7.792 ± 0.252
5.919GluLys: 5.919 ± 0.276
7.093GluLeu: 7.093 ± 0.25
1.555GluMet: 1.555 ± 0.116
5.077GluAsn: 5.077 ± 0.21
2.096GluPro: 2.096 ± 0.17
3.075GluGln: 3.075 ± 0.17
2.405GluArg: 2.405 ± 0.187
2.7GluSer: 2.7 ± 0.13
3.478GluThr: 3.478 ± 0.207
4.421GluVal: 4.421 ± 0.212
0.605GluTrp: 0.605 ± 0.065
2.945GluTyr: 2.945 ± 0.157
0.0GluXaa: 0.0 ± 0.0
Phe
2.398PheAla: 2.398 ± 0.138
0.554PheCys: 0.554 ± 0.062
2.996PheAsp: 2.996 ± 0.17
3.017PheGlu: 3.017 ± 0.169
2.045PhePhe: 2.045 ± 0.15
2.736PheGly: 2.736 ± 0.141
0.526PheHis: 0.526 ± 0.064
4.551PheIle: 4.551 ± 0.198
3.341PheLys: 3.341 ± 0.16
3.687PheLeu: 3.687 ± 0.202
1.167PheMet: 1.167 ± 0.112
2.974PheAsn: 2.974 ± 0.163
1.282PhePro: 1.282 ± 0.1
1.015PheGln: 1.015 ± 0.085
1.44PheArg: 1.44 ± 0.099
3.19PheSer: 3.19 ± 0.163
2.549PheThr: 2.549 ± 0.146
3.039PheVal: 3.039 ± 0.167
0.418PheTrp: 0.418 ± 0.057
1.822PheTyr: 1.822 ± 0.137
0.0PheXaa: 0.0 ± 0.0
Gly
4.537GlyAla: 4.537 ± 0.221
0.864GlyCys: 0.864 ± 0.108
3.464GlyAsp: 3.464 ± 0.174
4.335GlyGlu: 4.335 ± 0.212
2.736GlyPhe: 2.736 ± 0.175
4.4GlyGly: 4.4 ± 0.225
1.037GlyHis: 1.037 ± 0.098
6.085GlyIle: 6.085 ± 0.246
4.616GlyLys: 4.616 ± 0.191
5.127GlyLeu: 5.127 ± 0.272
2.002GlyMet: 2.002 ± 0.135
3.277GlyAsn: 3.277 ± 0.18
1.181GlyPro: 1.181 ± 0.105
1.944GlyGln: 1.944 ± 0.124
2.117GlyArg: 2.117 ± 0.138
3.334GlySer: 3.334 ± 0.171
3.55GlyThr: 3.55 ± 0.247
4.429GlyVal: 4.429 ± 0.223
0.533GlyTrp: 0.533 ± 0.069
2.794GlyTyr: 2.794 ± 0.177
0.0GlyXaa: 0.0 ± 0.0
His
0.756HisAla: 0.756 ± 0.068
0.166HisCys: 0.166 ± 0.04
0.879HisAsp: 0.879 ± 0.093
0.821HisGlu: 0.821 ± 0.082
0.735HisPhe: 0.735 ± 0.07
0.943HisGly: 0.943 ± 0.092
0.266HisHis: 0.266 ± 0.045
1.491HisIle: 1.491 ± 0.128
1.08HisLys: 1.08 ± 0.081
1.311HisLeu: 1.311 ± 0.105
0.418HisMet: 0.418 ± 0.05
0.929HisAsn: 0.929 ± 0.082
0.54HisPro: 0.54 ± 0.069
0.49HisGln: 0.49 ± 0.058
0.49HisArg: 0.49 ± 0.057
0.843HisSer: 0.843 ± 0.083
0.785HisThr: 0.785 ± 0.085
0.763HisVal: 0.763 ± 0.082
0.194HisTrp: 0.194 ± 0.042
0.583HisTyr: 0.583 ± 0.075
0.0HisXaa: 0.0 ± 0.0
Ile
6.171IleAla: 6.171 ± 0.247
1.231IleCys: 1.231 ± 0.091
6.078IleAsp: 6.078 ± 0.217
7.741IleGlu: 7.741 ± 0.239
4.501IlePhe: 4.501 ± 0.232
6.625IleGly: 6.625 ± 0.28
1.059IleHis: 1.059 ± 0.089
9.664IleIle: 9.664 ± 0.38
7.518IleLys: 7.518 ± 0.277
8.886IleLeu: 8.886 ± 0.341
2.348IleMet: 2.348 ± 0.145
5.948IleAsn: 5.948 ± 0.2
3.19IlePro: 3.19 ± 0.156
2.412IleGln: 2.412 ± 0.155
2.765IleArg: 2.765 ± 0.155
6.827IleSer: 6.827 ± 0.277
6.056IleThr: 6.056 ± 0.282
5.898IleVal: 5.898 ± 0.263
0.706IleTrp: 0.706 ± 0.083
3.608IleTyr: 3.608 ± 0.144
0.0IleXaa: 0.0 ± 0.0
Lys
3.781LysAla: 3.781 ± 0.192
0.655LysCys: 0.655 ± 0.064
4.638LysAsp: 4.638 ± 0.2
7.086LysGlu: 7.086 ± 0.343
3.118LysPhe: 3.118 ± 0.16
3.968LysGly: 3.968 ± 0.172
1.037LysHis: 1.037 ± 0.1
7.216LysIle: 7.216 ± 0.322
6.805LysLys: 6.805 ± 0.36
6.855LysLeu: 6.855 ± 0.302
2.088LysMet: 2.088 ± 0.127
5.869LysAsn: 5.869 ± 0.255
2.11LysPro: 2.11 ± 0.129
2.729LysGln: 2.729 ± 0.15
2.168LysArg: 2.168 ± 0.152
4.58LysSer: 4.58 ± 0.162
4.501LysThr: 4.501 ± 0.254
4.659LysVal: 4.659 ± 0.234
0.72LysTrp: 0.72 ± 0.128
3.507LysTyr: 3.507 ± 0.168
0.0LysXaa: 0.0 ± 0.0
Leu
5.386LeuAla: 5.386 ± 0.21
1.138LeuCys: 1.138 ± 0.097
5.329LeuAsp: 5.329 ± 0.186
7.028LeuGlu: 7.028 ± 0.281
3.831LeuPhe: 3.831 ± 0.17
5.408LeuGly: 5.408 ± 0.232
1.131LeuHis: 1.131 ± 0.104
7.871LeuIle: 7.871 ± 0.317
7.28LeuLys: 7.28 ± 0.272
7.576LeuLeu: 7.576 ± 0.354
2.146LeuMet: 2.146 ± 0.137
5.264LeuAsn: 5.264 ± 0.264
2.931LeuPro: 2.931 ± 0.164
2.578LeuGln: 2.578 ± 0.134
2.614LeuArg: 2.614 ± 0.155
6.438LeuSer: 6.438 ± 0.278
5.538LeuThr: 5.538 ± 0.228
5.343LeuVal: 5.343 ± 0.251
0.605LeuTrp: 0.605 ± 0.069
3.363LeuTyr: 3.363 ± 0.18
0.0LeuXaa: 0.0 ± 0.0
Met
1.743MetAla: 1.743 ± 0.12
0.216MetCys: 0.216 ± 0.04
1.375MetAsp: 1.375 ± 0.084
1.627MetGlu: 1.627 ± 0.121
1.03MetPhe: 1.03 ± 0.103
1.483MetGly: 1.483 ± 0.105
0.389MetHis: 0.389 ± 0.053
2.088MetIle: 2.088 ± 0.142
1.966MetLys: 1.966 ± 0.131
2.398MetLeu: 2.398 ± 0.148
0.648MetMet: 0.648 ± 0.082
1.375MetAsn: 1.375 ± 0.117
1.109MetPro: 1.109 ± 0.119
0.972MetGln: 0.972 ± 0.09
0.85MetArg: 0.85 ± 0.094
1.678MetSer: 1.678 ± 0.124
1.505MetThr: 1.505 ± 0.111
1.57MetVal: 1.57 ± 0.114
0.187MetTrp: 0.187 ± 0.035
1.066MetTyr: 1.066 ± 0.101
0.0MetXaa: 0.0 ± 0.0
Asn
3.579AsnAla: 3.579 ± 0.295
0.763AsnCys: 0.763 ± 0.079
3.154AsnAsp: 3.154 ± 0.162
4.069AsnGlu: 4.069 ± 0.179
2.873AsnPhe: 2.873 ± 0.162
3.982AsnGly: 3.982 ± 0.266
0.828AsnHis: 0.828 ± 0.086
7.079AsnIle: 7.079 ± 0.259
5.365AsnLys: 5.365 ± 0.281
5.725AsnLeu: 5.725 ± 0.218
1.548AsnMet: 1.548 ± 0.108
4.45AsnAsn: 4.45 ± 0.214
2.088AsnPro: 2.088 ± 0.137
1.829AsnGln: 1.829 ± 0.122
1.591AsnArg: 1.591 ± 0.098
3.572AsnSer: 3.572 ± 0.174
3.961AsnThr: 3.961 ± 0.247
3.687AsnVal: 3.687 ± 0.185
0.497AsnTrp: 0.497 ± 0.067
2.657AsnTyr: 2.657 ± 0.17
0.0AsnXaa: 0.0 ± 0.0
Pro
1.851ProAla: 1.851 ± 0.135
0.317ProCys: 0.317 ± 0.056
1.75ProAsp: 1.75 ± 0.125
2.564ProGlu: 2.564 ± 0.164
1.282ProPhe: 1.282 ± 0.097
1.462ProGly: 1.462 ± 0.111
0.612ProHis: 0.612 ± 0.057
3.06ProIle: 3.06 ± 0.175
2.081ProLys: 2.081 ± 0.114
2.614ProLeu: 2.614 ± 0.147
0.59ProMet: 0.59 ± 0.079
2.254ProAsn: 2.254 ± 0.137
0.85ProPro: 0.85 ± 0.099
0.987ProGln: 0.987 ± 0.099
0.864ProArg: 0.864 ± 0.078
1.959ProSer: 1.959 ± 0.117
2.038ProThr: 2.038 ± 0.133
1.944ProVal: 1.944 ± 0.131
0.259ProTrp: 0.259 ± 0.047
1.462ProTyr: 1.462 ± 0.092
0.0ProXaa: 0.0 ± 0.0
Gln
1.894GlnAla: 1.894 ± 0.173
0.194GlnCys: 0.194 ± 0.038
1.635GlnAsp: 1.635 ± 0.119
2.204GlnGlu: 2.204 ± 0.123
1.152GlnPhe: 1.152 ± 0.118
1.534GlnGly: 1.534 ± 0.105
0.461GlnHis: 0.461 ± 0.054
3.14GlnIle: 3.14 ± 0.162
2.715GlnLys: 2.715 ± 0.159
2.535GlnLeu: 2.535 ± 0.137
1.08GlnMet: 1.08 ± 0.105
2.556GlnAsn: 2.556 ± 0.157
0.907GlnPro: 0.907 ± 0.071
1.368GlnGln: 1.368 ± 0.145
1.095GlnArg: 1.095 ± 0.105
1.642GlnSer: 1.642 ± 0.124
2.002GlnThr: 2.002 ± 0.139
1.995GlnVal: 1.995 ± 0.133
0.23GlnTrp: 0.23 ± 0.064
1.303GlnTyr: 1.303 ± 0.084
0.0GlnXaa: 0.0 ± 0.0
Arg
2.232ArgAla: 2.232 ± 0.133
0.317ArgCys: 0.317 ± 0.054
1.627ArgAsp: 1.627 ± 0.108
2.391ArgGlu: 2.391 ± 0.208
1.584ArgPhe: 1.584 ± 0.101
1.685ArgGly: 1.685 ± 0.142
0.432ArgHis: 0.432 ± 0.061
2.686ArgIle: 2.686 ± 0.136
2.412ArgLys: 2.412 ± 0.163
2.664ArgLeu: 2.664 ± 0.173
0.922ArgMet: 0.922 ± 0.086
1.527ArgAsn: 1.527 ± 0.086
0.994ArgPro: 0.994 ± 0.088
1.239ArgGln: 1.239 ± 0.113
1.087ArgArg: 1.087 ± 0.123
1.339ArgSer: 1.339 ± 0.104
1.555ArgThr: 1.555 ± 0.121
1.879ArgVal: 1.879 ± 0.152
0.23ArgTrp: 0.23 ± 0.04
1.167ArgTyr: 1.167 ± 0.093
0.0ArgXaa: 0.0 ± 0.0
Ser
3.55SerAla: 3.55 ± 0.172
0.619SerCys: 0.619 ± 0.066
3.075SerAsp: 3.075 ± 0.165
3.442SerGlu: 3.442 ± 0.154
2.996SerPhe: 2.996 ± 0.162
3.997SerGly: 3.997 ± 0.19
0.907SerHis: 0.907 ± 0.084
6.409SerIle: 6.409 ± 0.257
4.277SerLys: 4.277 ± 0.18
5.473SerLeu: 5.473 ± 0.24
1.325SerMet: 1.325 ± 0.1
3.442SerAsn: 3.442 ± 0.161
1.75SerPro: 1.75 ± 0.092
1.916SerGln: 1.916 ± 0.133
1.642SerArg: 1.642 ± 0.118
4.126SerSer: 4.126 ± 0.213
3.723SerThr: 3.723 ± 0.218
3.946SerVal: 3.946 ± 0.202
0.504SerTrp: 0.504 ± 0.059
2.513SerTyr: 2.513 ± 0.115
0.0SerXaa: 0.0 ± 0.0
Thr
4.097ThrAla: 4.097 ± 0.541
0.482ThrCys: 0.482 ± 0.055
3.478ThrAsp: 3.478 ± 0.218
3.485ThrGlu: 3.485 ± 0.17
2.636ThrPhe: 2.636 ± 0.186
3.881ThrGly: 3.881 ± 0.227
1.073ThrHis: 1.073 ± 0.103
6.15ThrIle: 6.15 ± 0.241
4.45ThrLys: 4.45 ± 0.224
5.487ThrLeu: 5.487 ± 0.225
1.282ThrMet: 1.282 ± 0.095
3.687ThrAsn: 3.687 ± 0.2
2.225ThrPro: 2.225 ± 0.152
2.11ThrGln: 2.11 ± 0.217
1.375ThrArg: 1.375 ± 0.104
3.809ThrSer: 3.809 ± 0.186
4.061ThrThr: 4.061 ± 0.28
3.795ThrVal: 3.795 ± 0.229
0.346ThrTrp: 0.346 ± 0.075
2.06ThrTyr: 2.06 ± 0.152
0.0ThrXaa: 0.0 ± 0.0
Val
4.155ValAla: 4.155 ± 0.19
0.922ValCys: 0.922 ± 0.11
3.917ValAsp: 3.917 ± 0.242
4.328ValGlu: 4.328 ± 0.191
2.65ValPhe: 2.65 ± 0.154
4.306ValGly: 4.306 ± 0.223
0.778ValHis: 0.778 ± 0.09
5.545ValIle: 5.545 ± 0.219
4.177ValLys: 4.177 ± 0.211
5.494ValLeu: 5.494 ± 0.18
1.491ValMet: 1.491 ± 0.105
3.399ValAsn: 3.399 ± 0.172
2.326ValPro: 2.326 ± 0.136
1.512ValGln: 1.512 ± 0.119
2.031ValArg: 2.031 ± 0.151
4.436ValSer: 4.436 ± 0.207
3.896ValThr: 3.896 ± 0.245
4.306ValVal: 4.306 ± 0.196
0.49ValTrp: 0.49 ± 0.056
2.398ValTyr: 2.398 ± 0.115
0.0ValXaa: 0.0 ± 0.0
Trp
0.504TrpAla: 0.504 ± 0.063
0.13TrpCys: 0.13 ± 0.032
0.482TrpAsp: 0.482 ± 0.063
0.569TrpGlu: 0.569 ± 0.07
0.346TrpPhe: 0.346 ± 0.054
0.713TrpGly: 0.713 ± 0.089
0.122TrpHis: 0.122 ± 0.03
0.691TrpIle: 0.691 ± 0.064
0.497TrpLys: 0.497 ± 0.058
0.482TrpLeu: 0.482 ± 0.063
0.252TrpMet: 0.252 ± 0.043
0.504TrpAsn: 0.504 ± 0.071
0.187TrpPro: 0.187 ± 0.039
0.338TrpGln: 0.338 ± 0.044
0.302TrpArg: 0.302 ± 0.049
0.49TrpSer: 0.49 ± 0.063
0.389TrpThr: 0.389 ± 0.063
0.598TrpVal: 0.598 ± 0.144
0.072TrpTrp: 0.072 ± 0.026
0.346TrpTyr: 0.346 ± 0.059
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.319TyrAla: 2.319 ± 0.131
0.533TyrCys: 0.533 ± 0.056
2.405TyrAsp: 2.405 ± 0.168
2.808TyrGlu: 2.808 ± 0.163
2.045TyrPhe: 2.045 ± 0.122
2.506TyrGly: 2.506 ± 0.137
0.583TyrHis: 0.583 ± 0.07
4.141TyrIle: 4.141 ± 0.198
3.212TyrLys: 3.212 ± 0.159
3.514TyrLeu: 3.514 ± 0.216
1.073TyrMet: 1.073 ± 0.09
2.794TyrAsn: 2.794 ± 0.156
1.231TyrPro: 1.231 ± 0.087
1.21TyrGln: 1.21 ± 0.102
1.282TyrArg: 1.282 ± 0.083
2.153TyrSer: 2.153 ± 0.13
2.254TyrThr: 2.254 ± 0.127
2.247TyrVal: 2.247 ± 0.139
0.367TyrTrp: 0.367 ± 0.051
1.851TyrTyr: 1.851 ± 0.13
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 477 proteins (138868 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski