Thin paspalum asymptomatic virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Panicovirus

Average proteome isoelectric point is 7.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S4WHA6|S4WHA6_9TOMB Capsid protein OS=Thin paspalum asymptomatic virus OX=1352511 PE=3 SV=1
MM1 pKa = 7.6EE2 pKa = 5.19SFVSRR7 pKa = 11.84ISEE10 pKa = 4.09ALSSCDD16 pKa = 4.77ANLDD20 pKa = 3.61YY21 pKa = 11.57DD22 pKa = 4.65KK23 pKa = 11.03MLLILKK29 pKa = 8.7HH30 pKa = 6.65CPDD33 pKa = 4.83KK34 pKa = 11.1LLSWASGIEE43 pKa = 3.95GLQTHH48 pKa = 5.38QLKK51 pKa = 10.33VAITHH56 pKa = 5.75LVKK59 pKa = 10.29TEE61 pKa = 3.53FHH63 pKa = 5.84TCKK66 pKa = 10.39HH67 pKa = 6.11LLTTMSATTADD78 pKa = 4.18YY79 pKa = 10.78IKK81 pKa = 10.35MFMEE85 pKa = 4.69LSPEE89 pKa = 3.79QEE91 pKa = 4.3VIINTGGTVSWWPKK105 pKa = 9.94LIRR108 pKa = 11.84DD109 pKa = 3.47IPEE112 pKa = 3.81WFTDD116 pKa = 4.49KK117 pKa = 10.61IPHH120 pKa = 6.57YY121 pKa = 9.81ILQIVDD127 pKa = 5.75FILRR131 pKa = 11.84WVSRR135 pKa = 11.84ITGKK139 pKa = 9.72VRR141 pKa = 11.84PSSPKK146 pKa = 9.64EE147 pKa = 4.08SNSSSTRR154 pKa = 11.84PSWLRR159 pKa = 11.84LLVEE163 pKa = 4.35VGLVAAAWYY172 pKa = 9.66GVYY175 pKa = 10.2KK176 pKa = 10.41YY177 pKa = 10.44ISLSEE182 pKa = 4.18EE183 pKa = 3.5IVRR186 pKa = 11.84RR187 pKa = 11.84DD188 pKa = 3.49QAQTRR193 pKa = 11.84PDD195 pKa = 3.34HH196 pKa = 6.32MRR198 pKa = 11.84QCYY201 pKa = 8.61EE202 pKa = 3.76QVKK205 pKa = 10.36EE206 pKa = 4.36SLEE209 pKa = 3.97EE210 pKa = 3.73QNNRR214 pKa = 11.84FIHH217 pKa = 6.89DD218 pKa = 3.49RR219 pKa = 11.84MEE221 pKa = 4.18EE222 pKa = 4.11EE223 pKa = 3.98LTKK226 pKa = 10.93LVIEE230 pKa = 4.31PAEE233 pKa = 3.92IDD235 pKa = 3.49EE236 pKa = 5.12DD237 pKa = 3.96GKK239 pKa = 10.2EE240 pKa = 4.11VKK242 pKa = 10.46PEE244 pKa = 3.59VSQYY248 pKa = 11.01LVRR251 pKa = 11.84HH252 pKa = 5.82HH253 pKa = 6.64GKK255 pKa = 9.77FVRR258 pKa = 11.84ALVTMAKK265 pKa = 10.16NEE267 pKa = 3.98FAGVPKK273 pKa = 9.12PTEE276 pKa = 4.15ANQLAVWRR284 pKa = 11.84FLYY287 pKa = 9.84RR288 pKa = 11.84QCDD291 pKa = 3.44KK292 pKa = 11.25RR293 pKa = 11.84GVNPTDD299 pKa = 3.45TQKK302 pKa = 11.05SISAALPFVFLPSAYY317 pKa = 10.34DD318 pKa = 3.28QDD320 pKa = 3.67QAITMNCDD328 pKa = 3.21DD329 pKa = 5.01AKK331 pKa = 11.47EE332 pKa = 3.94MLQRR336 pKa = 11.84YY337 pKa = 9.43ADD339 pKa = 3.73TFRR342 pKa = 11.84YY343 pKa = 6.1TTPLQKK349 pKa = 10.41LVHH352 pKa = 6.18NPLMGKK358 pKa = 8.59HH359 pKa = 3.98WVAWARR365 pKa = 11.84SIFISDD371 pKa = 3.99PEE373 pKa = 4.25TGLRR377 pKa = 11.84FAKK380 pKa = 10.5

Molecular weight: 43.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S4WHA6|S4WHA6_9TOMB Capsid protein OS=Thin paspalum asymptomatic virus OX=1352511 PE=3 SV=1
MM1 pKa = 7.18NRR3 pKa = 11.84NNGAANARR11 pKa = 11.84RR12 pKa = 11.84GGKK15 pKa = 9.25RR16 pKa = 11.84APQSGARR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84ARR27 pKa = 11.84GKK29 pKa = 9.63SVEE32 pKa = 4.09RR33 pKa = 11.84RR34 pKa = 11.84AAPIQYY40 pKa = 7.99VTTVGPSQPRR50 pKa = 11.84MGTGQGWQRR59 pKa = 11.84ISHH62 pKa = 5.37QEE64 pKa = 3.6IILQVTASTTSDD76 pKa = 3.0SVQIVPIIPRR86 pKa = 11.84LSLSTAEE93 pKa = 3.99KK94 pKa = 10.16PIYY97 pKa = 9.69QGSAPHH103 pKa = 6.25LRR105 pKa = 11.84QMGDD109 pKa = 2.63AFSIHH114 pKa = 6.17RR115 pKa = 11.84WRR117 pKa = 11.84SLSFEE122 pKa = 4.41WVPSCPTTTPGNLVLRR138 pKa = 11.84FYY140 pKa = 10.52PSYY143 pKa = 10.13STPTPKK149 pKa = 10.8LLTDD153 pKa = 3.39IMDD156 pKa = 4.04SEE158 pKa = 4.87SLVIVPSISGAIYY171 pKa = 9.96RR172 pKa = 11.84PKK174 pKa = 10.11IDD176 pKa = 3.53TRR178 pKa = 11.84AQSPEE183 pKa = 3.83LRR185 pKa = 11.84NISITGFSALSDD197 pKa = 3.53EE198 pKa = 4.82DD199 pKa = 4.22KK200 pKa = 11.61GDD202 pKa = 3.7FSVGRR207 pKa = 11.84LVVGASKK214 pKa = 10.44QAVGLQLGLLRR225 pKa = 11.84MSYY228 pKa = 10.51VIEE231 pKa = 4.12LRR233 pKa = 11.84GATAGSGASAA243 pKa = 3.97

Molecular weight: 26.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1834

59

947

305.7

34.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.143 ± 0.962

1.799 ± 0.37

4.689 ± 0.39

6.107 ± 0.65

3.381 ± 0.347

5.289 ± 0.795

2.944 ± 0.499

5.889 ± 0.375

6.107 ± 1.038

8.615 ± 0.489

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.454 ± 0.239

3.053 ± 0.436

6.107 ± 0.545

4.308 ± 0.364

6.707 ± 0.671

7.797 ± 0.98

5.671 ± 0.629

6.216 ± 0.328

1.963 ± 0.336

3.708 ± 0.577
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski