Amino acid dipepetide frequency for Chthoniobacter flavus Ellin428

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.202AlaAla: 13.202 ± 0.114	1.014AlaCys: 1.014 ± 0.025	5.465AlaAsp: 5.465 ± 0.052	6.638AlaGlu: 6.638 ± 0.083	3.939AlaPhe: 3.939 ± 0.047	8.909AlaGly: 8.909 ± 0.093	2.323AlaHis: 2.323 ± 0.041	5.206AlaIle: 5.206 ± 0.054	4.491AlaLys: 4.491 ± 0.06	11.088AlaLeu: 11.088 ± 0.108	2.338AlaMet: 2.338 ± 0.04	3.523AlaAsn: 3.523 ± 0.075	5.621AlaPro: 5.621 ± 0.067	4.075AlaGln: 4.075 ± 0.058	6.689AlaArg: 6.689 ± 0.082	6.064AlaSer: 6.064 ± 0.067	6.51AlaThr: 6.51 ± 0.089	7.198AlaVal: 7.198 ± 0.061	1.596AlaTrp: 1.596 ± 0.03	2.202AlaTyr: 2.202 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	1.058CysAla: 1.058 ± 0.025	0.148CysCys: 0.148 ± 0.009	0.522CysAsp: 0.522 ± 0.017	0.549CysGlu: 0.549 ± 0.017	0.442CysPhe: 0.442 ± 0.016	0.966CysGly: 0.966 ± 0.022	0.385CysHis: 0.385 ± 0.015	0.419CysIle: 0.419 ± 0.015	0.262CysLys: 0.262 ± 0.011	0.975CysLeu: 0.975 ± 0.022	0.162CysMet: 0.162 ± 0.009	0.245CysAsn: 0.245 ± 0.011	0.494CysPro: 0.494 ± 0.016	0.321CysGln: 0.321 ± 0.012	0.643CysArg: 0.643 ± 0.018	0.549CysSer: 0.549 ± 0.017	0.472CysThr: 0.472 ± 0.014	0.692CysVal: 0.692 ± 0.019	0.162CysTrp: 0.162 ± 0.008	0.287CysTyr: 0.287 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	5.571AspAla: 5.571 ± 0.055	0.468AspCys: 0.468 ± 0.014	2.708AspAsp: 2.708 ± 0.041	3.041AspGlu: 3.041 ± 0.052	2.511AspPhe: 2.511 ± 0.04	4.99AspGly: 4.99 ± 0.06	1.302AspHis: 1.302 ± 0.03	2.368AspIle: 2.368 ± 0.031	1.963AspLys: 1.963 ± 0.039	5.23AspLeu: 5.23 ± 0.056	0.854AspMet: 0.854 ± 0.019	1.501AspAsn: 1.501 ± 0.036	3.356AspPro: 3.356 ± 0.043	1.729AspGln: 1.729 ± 0.028	3.141AspArg: 3.141 ± 0.046	2.757AspSer: 2.757 ± 0.037	2.667AspThr: 2.667 ± 0.038	3.474AspVal: 3.474 ± 0.037	1.028AspTrp: 1.028 ± 0.023	1.565AspTyr: 1.565 ± 0.025	0.0AspXaa: 0.0 ± 0.0
Glu	6.069GluAla: 6.069 ± 0.081	0.456GluCys: 0.456 ± 0.015	2.469GluAsp: 2.469 ± 0.044	3.483GluGlu: 3.483 ± 0.066	2.247GluPhe: 2.247 ± 0.033	3.687GluGly: 3.687 ± 0.047	1.422GluHis: 1.422 ± 0.03	3.367GluIle: 3.367 ± 0.046	3.298GluLys: 3.298 ± 0.052	5.615GluLeu: 5.615 ± 0.075	1.421GluMet: 1.421 ± 0.029	1.89GluAsn: 1.89 ± 0.027	2.762GluPro: 2.762 ± 0.046	2.274GluGln: 2.274 ± 0.04	4.051GluArg: 4.051 ± 0.068	2.954GluSer: 2.954 ± 0.043	3.249GluThr: 3.249 ± 0.054	3.836GluVal: 3.836 ± 0.051	0.899GluTrp: 0.899 ± 0.021	1.305GluTyr: 1.305 ± 0.028	0.0GluXaa: 0.0 ± 0.0
Phe	4.361PheAla: 4.361 ± 0.041	0.498PheCys: 0.498 ± 0.017	2.52PheAsp: 2.52 ± 0.035	2.101PheGlu: 2.101 ± 0.038	1.742PhePhe: 1.742 ± 0.033	3.449PheGly: 3.449 ± 0.042	1.012PheHis: 1.012 ± 0.025	1.747PheIle: 1.747 ± 0.034	1.214PheLys: 1.214 ± 0.028	3.935PheLeu: 3.935 ± 0.048	0.622PheMet: 0.622 ± 0.017	1.359PheAsn: 1.359 ± 0.034	1.983PhePro: 1.983 ± 0.033	1.354PheGln: 1.354 ± 0.024	2.493PheArg: 2.493 ± 0.04	2.577PheSer: 2.577 ± 0.041	2.74PheThr: 2.74 ± 0.051	2.834PheVal: 2.834 ± 0.038	0.628PheTrp: 0.628 ± 0.017	1.127PheTyr: 1.127 ± 0.025	0.0PheXaa: 0.0 ± 0.0
Gly	8.057GlyAla: 8.057 ± 0.119	0.849GlyCys: 0.849 ± 0.023	4.146GlyAsp: 4.146 ± 0.053	4.411GlyGlu: 4.411 ± 0.054	3.379GlyPhe: 3.379 ± 0.042	7.445GlyGly: 7.445 ± 0.155	1.958GlyHis: 1.958 ± 0.031	3.933GlyIle: 3.933 ± 0.047	4.062GlyLys: 4.062 ± 0.053	7.273GlyLeu: 7.273 ± 0.066	1.765GlyMet: 1.765 ± 0.029	3.158GlyAsn: 3.158 ± 0.1	3.192GlyPro: 3.192 ± 0.043	2.981GlyGln: 2.981 ± 0.043	4.86GlyArg: 4.86 ± 0.059	5.206GlySer: 5.206 ± 0.116	5.875GlyThr: 5.875 ± 0.219	5.661GlyVal: 5.661 ± 0.064	1.447GlyTrp: 1.447 ± 0.031	2.299GlyTyr: 2.299 ± 0.037	0.0GlyXaa: 0.0 ± 0.0
His	2.408HisAla: 2.408 ± 0.038	0.285HisCys: 0.285 ± 0.012	1.374HisAsp: 1.374 ± 0.027	1.365HisGlu: 1.365 ± 0.031	1.195HisPhe: 1.195 ± 0.025	2.133HisGly: 2.133 ± 0.034	0.801HisHis: 0.801 ± 0.022	0.982HisIle: 0.982 ± 0.021	0.618HisLys: 0.618 ± 0.016	2.335HisLeu: 2.335 ± 0.04	0.407HisMet: 0.407 ± 0.013	0.635HisAsn: 0.635 ± 0.016	1.573HisPro: 1.573 ± 0.031	0.736HisGln: 0.736 ± 0.02	1.633HisArg: 1.633 ± 0.035	1.284HisSer: 1.284 ± 0.028	1.2HisThr: 1.2 ± 0.028	1.553HisVal: 1.553 ± 0.036	0.475HisTrp: 0.475 ± 0.014	0.734HisTyr: 0.734 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	5.591IleAla: 5.591 ± 0.056	0.569IleCys: 0.569 ± 0.017	2.904IleAsp: 2.904 ± 0.035	3.061IleGlu: 3.061 ± 0.044	1.983IlePhe: 1.983 ± 0.03	4.044IleGly: 4.044 ± 0.054	1.156IleHis: 1.156 ± 0.025	2.244IleIle: 2.244 ± 0.037	1.593IleLys: 1.593 ± 0.031	4.258IleLeu: 4.258 ± 0.046	0.717IleMet: 0.717 ± 0.018	1.635IleAsn: 1.635 ± 0.057	2.589IlePro: 2.589 ± 0.043	1.522IleGln: 1.522 ± 0.023	2.864IleArg: 2.864 ± 0.045	3.019IleSer: 3.019 ± 0.047	3.154IleThr: 3.154 ± 0.066	3.749IleVal: 3.749 ± 0.045	0.61IleTrp: 0.61 ± 0.017	1.324IleTyr: 1.324 ± 0.024	0.0IleXaa: 0.0 ± 0.0
Lys	4.154LysAla: 4.154 ± 0.055	0.282LysCys: 0.282 ± 0.013	2.203LysAsp: 2.203 ± 0.04	2.395LysGlu: 2.395 ± 0.042	1.378LysPhe: 1.378 ± 0.027	2.747LysGly: 2.747 ± 0.043	0.858LysHis: 0.858 ± 0.023	2.246LysIle: 2.246 ± 0.033	2.22LysLys: 2.22 ± 0.047	4.134LysLeu: 4.134 ± 0.054	1.17LysMet: 1.17 ± 0.025	1.335LysAsn: 1.335 ± 0.026	2.715LysPro: 2.715 ± 0.053	1.444LysGln: 1.444 ± 0.03	2.31LysArg: 2.31 ± 0.041	2.326LysSer: 2.326 ± 0.038	2.484LysThr: 2.484 ± 0.033	2.76LysVal: 2.76 ± 0.044	0.591LysTrp: 0.591 ± 0.017	0.941LysTyr: 0.941 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	11.74LeuAla: 11.74 ± 0.091	1.129LeuCys: 1.129 ± 0.025	5.276LeuAsp: 5.276 ± 0.052	5.174LeuGlu: 5.174 ± 0.069	3.737LeuPhe: 3.737 ± 0.045	7.603LeuGly: 7.603 ± 0.071	2.437LeuHis: 2.437 ± 0.04	4.37LeuIle: 4.37 ± 0.046	3.784LeuLys: 3.784 ± 0.052	10.067LeuLeu: 10.067 ± 0.107	1.696LeuMet: 1.696 ± 0.03	3.27LeuAsn: 3.27 ± 0.073	5.88LeuPro: 5.88 ± 0.06	3.548LeuGln: 3.548 ± 0.042	7.041LeuArg: 7.041 ± 0.082	5.929LeuSer: 5.929 ± 0.06	6.298LeuThr: 6.298 ± 0.113	6.493LeuVal: 6.493 ± 0.053	1.328LeuTrp: 1.328 ± 0.03	2.13LeuTyr: 2.13 ± 0.031	0.0LeuXaa: 0.0 ± 0.0
Met	1.981MetAla: 1.981 ± 0.03	0.152MetCys: 0.152 ± 0.009	0.977MetAsp: 0.977 ± 0.022	1.053MetGlu: 1.053 ± 0.025	0.609MetPhe: 0.609 ± 0.017	1.361MetGly: 1.361 ± 0.024	0.488MetHis: 0.488 ± 0.015	1.013MetIle: 1.013 ± 0.026	1.257MetLys: 1.257 ± 0.025	1.984MetLeu: 1.984 ± 0.03	0.413MetMet: 0.413 ± 0.014	0.909MetAsn: 0.909 ± 0.02	1.332MetPro: 1.332 ± 0.025	0.751MetGln: 0.751 ± 0.021	1.368MetArg: 1.368 ± 0.03	1.306MetSer: 1.306 ± 0.025	1.274MetThr: 1.274 ± 0.021	1.227MetVal: 1.227 ± 0.024	0.251MetTrp: 0.251 ± 0.012	0.295MetTyr: 0.295 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	3.642AsnAla: 3.642 ± 0.07	0.302AsnCys: 0.302 ± 0.013	1.641AsnAsp: 1.641 ± 0.033	1.55AsnGlu: 1.55 ± 0.027	1.4AsnPhe: 1.4 ± 0.03	3.659AsnGly: 3.659 ± 0.12	0.724AsnHis: 0.724 ± 0.018	1.629AsnIle: 1.629 ± 0.042	0.944AsnLys: 0.944 ± 0.023	3.253AsnLeu: 3.253 ± 0.06	0.505AsnMet: 0.505 ± 0.014	1.32AsnAsn: 1.32 ± 0.066	2.302AsnPro: 2.302 ± 0.036	1.117AsnGln: 1.117 ± 0.029	1.831AsnArg: 1.831 ± 0.03	2.1AsnSer: 2.1 ± 0.073	2.112AsnThr: 2.112 ± 0.091	2.327AsnVal: 2.327 ± 0.043	0.545AsnTrp: 0.545 ± 0.015	0.955AsnTyr: 0.955 ± 0.022	0.0AsnXaa: 0.0 ± 0.0
Pro	6.556ProAla: 6.556 ± 0.071	0.396ProCys: 0.396 ± 0.013	3.381ProAsp: 3.381 ± 0.049	3.945ProGlu: 3.945 ± 0.061	1.979ProPhe: 1.979 ± 0.031	4.534ProGly: 4.534 ± 0.054	1.246ProHis: 1.246 ± 0.026	2.459ProIle: 2.459 ± 0.035	2.373ProLys: 2.373 ± 0.046	4.928ProLeu: 4.928 ± 0.059	1.093ProMet: 1.093 ± 0.027	1.986ProAsn: 1.986 ± 0.037	3.412ProPro: 3.412 ± 0.067	2.02ProGln: 2.02 ± 0.037	2.955ProArg: 2.955 ± 0.049	3.269ProSer: 3.269 ± 0.048	3.303ProThr: 3.303 ± 0.044	4.071ProVal: 4.071 ± 0.05	0.843ProTrp: 0.843 ± 0.022	1.203ProTyr: 1.203 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	3.599GlnAla: 3.599 ± 0.052	0.32GlnCys: 0.32 ± 0.011	1.401GlnAsp: 1.401 ± 0.029	1.703GlnGlu: 1.703 ± 0.033	1.481GlnPhe: 1.481 ± 0.026	2.382GlnGly: 2.382 ± 0.04	0.896GlnHis: 0.896 ± 0.024	2.028GlnIle: 2.028 ± 0.03	1.646GlnLys: 1.646 ± 0.033	3.703GlnLeu: 3.703 ± 0.045	0.891GlnMet: 0.891 ± 0.019	1.184GlnAsn: 1.184 ± 0.024	2.275GlnPro: 2.275 ± 0.033	1.671GlnGln: 1.671 ± 0.046	2.818GlnArg: 2.818 ± 0.044	2.013GlnSer: 2.013 ± 0.033	2.13GlnThr: 2.13 ± 0.039	2.373GlnVal: 2.373 ± 0.029	0.626GlnTrp: 0.626 ± 0.017	0.795GlnTyr: 0.795 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	6.261ArgAla: 6.261 ± 0.077	0.694ArgCys: 0.694 ± 0.019	3.48ArgAsp: 3.48 ± 0.048	4.329ArgGlu: 4.329 ± 0.064	2.735ArgPhe: 2.735 ± 0.044	4.372ArgGly: 4.372 ± 0.051	1.54ArgHis: 1.54 ± 0.029	3.219ArgIle: 3.219 ± 0.04	2.501ArgLys: 2.501 ± 0.042	6.669ArgLeu: 6.669 ± 0.075	1.52ArgMet: 1.52 ± 0.03	1.881ArgAsn: 1.881 ± 0.029	3.071ArgPro: 3.071 ± 0.045	2.512ArgGln: 2.512 ± 0.044	4.78ArgArg: 4.78 ± 0.071	3.315ArgSer: 3.315 ± 0.054	3.264ArgThr: 3.264 ± 0.045	4.312ArgVal: 4.312 ± 0.054	1.164ArgTrp: 1.164 ± 0.023	1.71ArgTyr: 1.71 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	6.242SerAla: 6.242 ± 0.06	0.499SerCys: 0.499 ± 0.018	2.901SerAsp: 2.901 ± 0.034	2.924SerGlu: 2.924 ± 0.046	2.428SerPhe: 2.428 ± 0.033	5.932SerGly: 5.932 ± 0.135	1.286SerHis: 1.286 ± 0.028	2.771SerIle: 2.771 ± 0.047	2.023SerLys: 2.023 ± 0.036	5.786SerLeu: 5.786 ± 0.057	1.219SerMet: 1.219 ± 0.024	2.02SerAsn: 2.02 ± 0.06	3.503SerPro: 3.503 ± 0.045	1.886SerGln: 1.886 ± 0.029	3.321SerArg: 3.321 ± 0.045	3.852SerSer: 3.852 ± 0.072	3.761SerThr: 3.761 ± 0.086	4.008SerVal: 4.008 ± 0.056	0.865SerTrp: 0.865 ± 0.022	1.473SerTyr: 1.473 ± 0.03	0.0SerXaa: 0.0 ± 0.0
Thr	6.597ThrAla: 6.597 ± 0.099	0.464ThrCys: 0.464 ± 0.015	2.909ThrAsp: 2.909 ± 0.04	2.798ThrGlu: 2.798 ± 0.042	2.574ThrPhe: 2.574 ± 0.06	5.786ThrGly: 5.786 ± 0.158	1.285ThrHis: 1.285 ± 0.026	3.291ThrIle: 3.291 ± 0.08	2.166ThrLys: 2.166 ± 0.033	6.626ThrLeu: 6.626 ± 0.145	1.076ThrMet: 1.076 ± 0.023	2.128ThrAsn: 2.128 ± 0.082	4.213ThrPro: 4.213 ± 0.054	1.881ThrGln: 1.881 ± 0.036	3.095ThrArg: 3.095 ± 0.042	3.599ThrSer: 3.599 ± 0.079	4.092ThrThr: 4.092 ± 0.116	4.653ThrVal: 4.653 ± 0.084	0.939ThrTrp: 0.939 ± 0.024	1.559ThrTyr: 1.559 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	7.424ValAla: 7.424 ± 0.058	0.755ValCys: 0.755 ± 0.02	3.683ValAsp: 3.683 ± 0.046	4.11ValGlu: 4.11 ± 0.055	2.739ValPhe: 2.739 ± 0.038	4.899ValGly: 4.899 ± 0.06	1.491ValHis: 1.491 ± 0.028	3.552ValIle: 3.552 ± 0.042	2.746ValLys: 2.746 ± 0.045	6.82ValLeu: 6.82 ± 0.06	1.367ValMet: 1.367 ± 0.028	2.413ValAsn: 2.413 ± 0.058	3.672ValPro: 3.672 ± 0.052	2.373ValGln: 2.373 ± 0.032	4.346ValArg: 4.346 ± 0.056	4.177ValSer: 4.177 ± 0.044	4.808ValThr: 4.808 ± 0.075	5.163ValVal: 5.163 ± 0.058	0.989ValTrp: 0.989 ± 0.02	1.547ValTyr: 1.547 ± 0.03	0.0ValXaa: 0.0 ± 0.0
Trp	1.219TrpAla: 1.219 ± 0.027	0.193TrpCys: 0.193 ± 0.01	0.763TrpAsp: 0.763 ± 0.018	0.775TrpGlu: 0.775 ± 0.019	0.635TrpPhe: 0.635 ± 0.02	0.958TrpGly: 0.958 ± 0.023	0.46TrpHis: 0.46 ± 0.016	0.773TrpIle: 0.773 ± 0.019	0.816TrpLys: 0.816 ± 0.023	1.801TrpLeu: 1.801 ± 0.035	0.414TrpMet: 0.414 ± 0.015	0.641TrpAsn: 0.641 ± 0.019	0.797TrpPro: 0.797 ± 0.019	0.773TrpGln: 0.773 ± 0.021	1.222TrpArg: 1.222 ± 0.027	0.957TrpSer: 0.957 ± 0.021	0.912TrpThr: 0.912 ± 0.023	0.972TrpVal: 0.972 ± 0.022	0.313TrpTrp: 0.313 ± 0.014	0.352TrpTyr: 0.352 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.36TyrAla: 2.36 ± 0.035	0.28TyrCys: 0.28 ± 0.011	1.502TyrAsp: 1.502 ± 0.033	1.349TyrGlu: 1.349 ± 0.028	1.162TyrPhe: 1.162 ± 0.026	2.098TyrGly: 2.098 ± 0.036	0.654TyrHis: 0.654 ± 0.019	0.967TyrIle: 0.967 ± 0.022	0.717TyrLys: 0.717 ± 0.019	2.449TyrLeu: 2.449 ± 0.041	0.349TyrMet: 0.349 ± 0.013	0.826TyrAsn: 0.826 ± 0.025	1.21TyrPro: 1.21 ± 0.028	0.97TyrGln: 0.97 ± 0.022	1.867TyrArg: 1.867 ± 0.036	1.447TyrSer: 1.447 ± 0.027	1.428TyrThr: 1.428 ± 0.033	1.719TyrVal: 1.719 ± 0.029	0.448TyrTrp: 0.448 ± 0.014	0.855TyrTyr: 0.855 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6705 proteins (2302626 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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