Amino acid dipepetide frequency for Humibacillus sp. DSM 29435

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.439AlaAla: 19.439 ± 0.204
0.953AlaCys: 0.953 ± 0.028
8.002AlaAsp: 8.002 ± 0.081
6.598AlaGlu: 6.598 ± 0.082
3.576AlaPhe: 3.576 ± 0.056
12.252AlaGly: 12.252 ± 0.111
2.665AlaHis: 2.665 ± 0.048
4.677AlaIle: 4.677 ± 0.071
2.773AlaLys: 2.773 ± 0.05
13.48AlaLeu: 13.48 ± 0.154
2.666AlaMet: 2.666 ± 0.04
2.306AlaAsn: 2.306 ± 0.044
6.048AlaPro: 6.048 ± 0.072
3.906AlaGln: 3.906 ± 0.055
9.354AlaArg: 9.354 ± 0.091
7.181AlaSer: 7.181 ± 0.079
8.109AlaThr: 8.109 ± 0.082
12.008AlaVal: 12.008 ± 0.107
1.928AlaTrp: 1.928 ± 0.044
2.346AlaTyr: 2.346 ± 0.043
0.0AlaXaa: 0.0 ± 0.0
Cys
0.805CysAla: 0.805 ± 0.022
0.074CysCys: 0.074 ± 0.007
0.48CysAsp: 0.48 ± 0.02
0.335CysGlu: 0.335 ± 0.014
0.231CysPhe: 0.231 ± 0.013
0.854CysGly: 0.854 ± 0.027
0.174CysHis: 0.174 ± 0.011
0.227CysIle: 0.227 ± 0.011
0.093CysLys: 0.093 ± 0.008
0.671CysLeu: 0.671 ± 0.021
0.099CysMet: 0.099 ± 0.008
0.128CysAsn: 0.128 ± 0.009
0.453CysPro: 0.453 ± 0.018
0.155CysGln: 0.155 ± 0.01
0.553CysArg: 0.553 ± 0.021
0.482CysSer: 0.482 ± 0.018
0.463CysThr: 0.463 ± 0.021
0.608CysVal: 0.608 ± 0.022
0.123CysTrp: 0.123 ± 0.011
0.152CysTyr: 0.152 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
7.687AspAla: 7.687 ± 0.08
0.369AspCys: 0.369 ± 0.018
4.586AspAsp: 4.586 ± 0.076
4.069AspGlu: 4.069 ± 0.065
1.624AspPhe: 1.624 ± 0.038
5.892AspGly: 5.892 ± 0.071
1.566AspHis: 1.566 ± 0.031
2.062AspIle: 2.062 ± 0.048
1.056AspLys: 1.056 ± 0.036
7.053AspLeu: 7.053 ± 0.067
0.74AspMet: 0.74 ± 0.024
0.925AspAsn: 0.925 ± 0.023
4.379AspPro: 4.379 ± 0.061
1.825AspGln: 1.825 ± 0.036
4.678AspArg: 4.678 ± 0.059
2.643AspSer: 2.643 ± 0.05
2.949AspThr: 2.949 ± 0.05
5.511AspVal: 5.511 ± 0.059
0.986AspTrp: 0.986 ± 0.029
1.176AspTyr: 1.176 ± 0.032
0.0AspXaa: 0.0 ± 0.0
Glu
6.693GluAla: 6.693 ± 0.083
0.308GluCys: 0.308 ± 0.013
1.855GluAsp: 1.855 ± 0.042
2.189GluGlu: 2.189 ± 0.053
1.328GluPhe: 1.328 ± 0.033
3.837GluGly: 3.837 ± 0.05
1.573GluHis: 1.573 ± 0.034
2.154GluIle: 2.154 ± 0.046
1.145GluLys: 1.145 ± 0.034
5.687GluLeu: 5.687 ± 0.067
0.939GluMet: 0.939 ± 0.026
1.043GluAsn: 1.043 ± 0.027
3.085GluPro: 3.085 ± 0.055
2.305GluGln: 2.305 ± 0.041
4.841GluArg: 4.841 ± 0.063
2.752GluSer: 2.752 ± 0.044
2.786GluThr: 2.786 ± 0.041
4.882GluVal: 4.882 ± 0.064
0.734GluTrp: 0.734 ± 0.021
0.835GluTyr: 0.835 ± 0.026
0.0GluXaa: 0.0 ± 0.0
Phe
3.793PheAla: 3.793 ± 0.049
0.257PheCys: 0.257 ± 0.014
2.179PheAsp: 2.179 ± 0.046
1.632PheGlu: 1.632 ± 0.035
0.95PhePhe: 0.95 ± 0.029
3.148PheGly: 3.148 ± 0.056
0.582PheHis: 0.582 ± 0.021
0.912PheIle: 0.912 ± 0.031
0.473PheLys: 0.473 ± 0.02
2.47PheLeu: 2.47 ± 0.044
0.442PheMet: 0.442 ± 0.018
0.622PheAsn: 0.622 ± 0.021
1.265PhePro: 1.265 ± 0.032
0.624PheGln: 0.624 ± 0.021
1.66PheArg: 1.66 ± 0.032
1.606PheSer: 1.606 ± 0.038
2.041PheThr: 2.041 ± 0.044
2.855PheVal: 2.855 ± 0.045
0.481PheTrp: 0.481 ± 0.02
0.619PheTyr: 0.619 ± 0.021
0.0PheXaa: 0.0 ± 0.0
Gly
10.605GlyAla: 10.605 ± 0.104
0.772GlyCys: 0.772 ± 0.027
5.268GlyAsp: 5.268 ± 0.072
4.53GlyGlu: 4.53 ± 0.057
2.993GlyPhe: 2.993 ± 0.052
8.217GlyGly: 8.217 ± 0.11
2.243GlyHis: 2.243 ± 0.041
3.931GlyIle: 3.931 ± 0.059
2.109GlyLys: 2.109 ± 0.046
9.792GlyLeu: 9.792 ± 0.101
1.883GlyMet: 1.883 ± 0.034
1.788GlyAsn: 1.788 ± 0.037
4.736GlyPro: 4.736 ± 0.074
2.958GlyGln: 2.958 ± 0.052
7.111GlyArg: 7.111 ± 0.08
6.068GlySer: 6.068 ± 0.079
5.489GlyThr: 5.489 ± 0.079
8.036GlyVal: 8.036 ± 0.09
1.836GlyTrp: 1.836 ± 0.041
2.176GlyTyr: 2.176 ± 0.039
0.0GlyXaa: 0.0 ± 0.0
His
2.477HisAla: 2.477 ± 0.047
0.196HisCys: 0.196 ± 0.013
1.747HisAsp: 1.747 ± 0.04
1.307HisGlu: 1.307 ± 0.025
0.582HisPhe: 0.582 ± 0.02
2.197HisGly: 2.197 ± 0.044
0.722HisHis: 0.722 ± 0.022
0.63HisIle: 0.63 ± 0.019
0.278HisLys: 0.278 ± 0.014
2.614HisLeu: 2.614 ± 0.042
0.274HisMet: 0.274 ± 0.014
0.373HisAsn: 0.373 ± 0.014
1.713HisPro: 1.713 ± 0.037
0.676HisGln: 0.676 ± 0.024
1.83HisArg: 1.83 ± 0.037
1.021HisSer: 1.021 ± 0.03
1.16HisThr: 1.16 ± 0.029
1.945HisVal: 1.945 ± 0.039
0.363HisTrp: 0.363 ± 0.015
0.44HisTyr: 0.44 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
5.28IleAla: 5.28 ± 0.065
0.286IleCys: 0.286 ± 0.015
2.985IleAsp: 2.985 ± 0.056
2.414IleGlu: 2.414 ± 0.044
0.955IlePhe: 0.955 ± 0.031
4.008IleGly: 4.008 ± 0.065
0.679IleHis: 0.679 ± 0.022
1.34IleIle: 1.34 ± 0.038
0.758IleLys: 0.758 ± 0.029
2.647IleLeu: 2.647 ± 0.05
0.528IleMet: 0.528 ± 0.021
0.793IleAsn: 0.793 ± 0.023
1.822IlePro: 1.822 ± 0.037
0.737IleGln: 0.737 ± 0.024
2.316IleArg: 2.316 ± 0.044
2.011IleSer: 2.011 ± 0.042
2.46IleThr: 2.46 ± 0.048
3.763IleVal: 3.763 ± 0.059
0.441IleTrp: 0.441 ± 0.016
0.632IleTyr: 0.632 ± 0.022
0.0IleXaa: 0.0 ± 0.0
Lys
2.744LysAla: 2.744 ± 0.056
0.093LysCys: 0.093 ± 0.009
1.009LysAsp: 1.009 ± 0.03
0.864LysGlu: 0.864 ± 0.029
0.462LysPhe: 0.462 ± 0.019
1.747LysGly: 1.747 ± 0.044
0.418LysHis: 0.418 ± 0.017
0.843LysIle: 0.843 ± 0.031
0.733LysLys: 0.733 ± 0.029
1.675LysLeu: 1.675 ± 0.038
0.388LysMet: 0.388 ± 0.019
0.523LysAsn: 0.523 ± 0.021
1.21LysPro: 1.21 ± 0.03
0.732LysGln: 0.732 ± 0.027
1.343LysArg: 1.343 ± 0.031
1.132LysSer: 1.132 ± 0.035
1.321LysThr: 1.321 ± 0.037
1.968LysVal: 1.968 ± 0.045
0.236LysTrp: 0.236 ± 0.013
0.419LysTyr: 0.419 ± 0.018
0.0LysXaa: 0.0 ± 0.0
Leu
14.443LeuAla: 14.443 ± 0.135
0.683LeuCys: 0.683 ± 0.022
6.659LeuAsp: 6.659 ± 0.07
4.986LeuGlu: 4.986 ± 0.068
2.629LeuPhe: 2.629 ± 0.05
9.564LeuGly: 9.564 ± 0.098
2.066LeuHis: 2.066 ± 0.036
3.367LeuIle: 3.367 ± 0.053
1.754LeuLys: 1.754 ± 0.043
10.332LeuLeu: 10.332 ± 0.118
1.683LeuMet: 1.683 ± 0.034
1.67LeuAsn: 1.67 ± 0.038
5.765LeuPro: 5.765 ± 0.07
2.343LeuGln: 2.343 ± 0.038
7.786LeuArg: 7.786 ± 0.083
5.903LeuSer: 5.903 ± 0.078
6.958LeuThr: 6.958 ± 0.067
10.136LeuVal: 10.136 ± 0.101
1.293LeuTrp: 1.293 ± 0.033
1.604LeuTyr: 1.604 ± 0.032
0.0LeuXaa: 0.0 ± 0.0
Met
2.397MetAla: 2.397 ± 0.045
0.12MetCys: 0.12 ± 0.01
0.75MetAsp: 0.75 ± 0.026
0.64MetGlu: 0.64 ± 0.022
0.509MetPhe: 0.509 ± 0.017
1.428MetGly: 1.428 ± 0.03
0.377MetHis: 0.377 ± 0.018
0.747MetIle: 0.747 ± 0.023
0.45MetLys: 0.45 ± 0.018
1.816MetLeu: 1.816 ± 0.038
0.325MetMet: 0.325 ± 0.013
0.465MetAsn: 0.465 ± 0.018
1.145MetPro: 1.145 ± 0.028
0.471MetGln: 0.471 ± 0.018
1.423MetArg: 1.423 ± 0.032
1.495MetSer: 1.495 ± 0.031
1.674MetThr: 1.674 ± 0.031
1.582MetVal: 1.582 ± 0.038
0.189MetTrp: 0.189 ± 0.011
0.268MetTyr: 0.268 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
2.354AsnAla: 2.354 ± 0.047
0.149AsnCys: 0.149 ± 0.011
1.188AsnAsp: 1.188 ± 0.033
0.918AsnGlu: 0.918 ± 0.031
0.53AsnPhe: 0.53 ± 0.021
1.932AsnGly: 1.932 ± 0.044
0.451AsnHis: 0.451 ± 0.016
0.7AsnIle: 0.7 ± 0.024
0.389AsnLys: 0.389 ± 0.02
1.962AsnLeu: 1.962 ± 0.039
0.289AsnMet: 0.289 ± 0.013
0.447AsnAsn: 0.447 ± 0.02
1.519AsnPro: 1.519 ± 0.035
0.606AsnGln: 0.606 ± 0.02
1.36AsnArg: 1.36 ± 0.032
0.852AsnSer: 0.852 ± 0.027
1.106AsnThr: 1.106 ± 0.03
1.655AsnVal: 1.655 ± 0.034
0.308AsnTrp: 0.308 ± 0.016
0.43AsnTyr: 0.43 ± 0.018
0.0AsnXaa: 0.0 ± 0.0
Pro
7.131ProAla: 7.131 ± 0.077
0.304ProCys: 0.304 ± 0.015
4.304ProAsp: 4.304 ± 0.057
3.305ProGlu: 3.305 ± 0.053
1.62ProPhe: 1.62 ± 0.031
5.712ProGly: 5.712 ± 0.067
1.157ProHis: 1.157 ± 0.029
1.739ProIle: 1.739 ± 0.034
1.112ProLys: 1.112 ± 0.034
4.95ProLeu: 4.95 ± 0.063
1.087ProMet: 1.087 ± 0.026
1.131ProAsn: 1.131 ± 0.028
3.003ProPro: 3.003 ± 0.06
1.617ProGln: 1.617 ± 0.036
3.472ProArg: 3.472 ± 0.053
3.633ProSer: 3.633 ± 0.045
3.935ProThr: 3.935 ± 0.053
5.233ProVal: 5.233 ± 0.068
0.918ProTrp: 0.918 ± 0.028
1.046ProTyr: 1.046 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
3.804GlnAla: 3.804 ± 0.055
0.193GlnCys: 0.193 ± 0.012
1.019GlnAsp: 1.019 ± 0.031
1.143GlnGlu: 1.143 ± 0.031
0.812GlnPhe: 0.812 ± 0.027
2.361GlnGly: 2.361 ± 0.044
0.748GlnHis: 0.748 ± 0.022
1.244GlnIle: 1.244 ± 0.031
0.62GlnLys: 0.62 ± 0.022
3.183GlnLeu: 3.183 ± 0.048
0.552GlnMet: 0.552 ± 0.018
0.603GlnAsn: 0.603 ± 0.02
1.75GlnPro: 1.75 ± 0.032
1.312GlnGln: 1.312 ± 0.038
2.497GlnArg: 2.497 ± 0.047
1.566GlnSer: 1.566 ± 0.032
1.658GlnThr: 1.658 ± 0.035
2.901GlnVal: 2.901 ± 0.047
0.563GlnTrp: 0.563 ± 0.021
0.481GlnTyr: 0.481 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
8.787ArgAla: 8.787 ± 0.095
0.53ArgCys: 0.53 ± 0.021
4.417ArgAsp: 4.417 ± 0.069
4.114ArgGlu: 4.114 ± 0.063
2.27ArgPhe: 2.27 ± 0.043
5.645ArgGly: 5.645 ± 0.063
1.934ArgHis: 1.934 ± 0.041
2.83ArgIle: 2.83 ± 0.045
1.294ArgLys: 1.294 ± 0.029
8.262ArgLeu: 8.262 ± 0.094
1.688ArgMet: 1.688 ± 0.036
1.294ArgAsn: 1.294 ± 0.03
4.316ArgPro: 4.316 ± 0.06
2.323ArgGln: 2.323 ± 0.043
6.92ArgArg: 6.92 ± 0.09
4.632ArgSer: 4.632 ± 0.066
4.238ArgThr: 4.238 ± 0.058
6.3ArgVal: 6.3 ± 0.075
1.364ArgTrp: 1.364 ± 0.032
1.555ArgTyr: 1.555 ± 0.034
0.0ArgXaa: 0.0 ± 0.0
Ser
7.111SerAla: 7.111 ± 0.073
0.401SerCys: 0.401 ± 0.019
3.392SerAsp: 3.392 ± 0.052
2.621SerGlu: 2.621 ± 0.046
1.84SerPhe: 1.84 ± 0.04
6.348SerGly: 6.348 ± 0.063
1.207SerHis: 1.207 ± 0.033
2.145SerIle: 2.145 ± 0.043
1.109SerLys: 1.109 ± 0.031
5.672SerLeu: 5.672 ± 0.076
1.389SerMet: 1.389 ± 0.032
1.141SerAsn: 1.141 ± 0.03
3.508SerPro: 3.508 ± 0.045
1.593SerGln: 1.593 ± 0.034
4.156SerArg: 4.156 ± 0.058
3.846SerSer: 3.846 ± 0.058
3.858SerThr: 3.858 ± 0.062
4.965SerVal: 4.965 ± 0.061
0.997SerTrp: 0.997 ± 0.03
1.139SerTyr: 1.139 ± 0.031
0.0SerXaa: 0.0 ± 0.0
Thr
7.857ThrAla: 7.857 ± 0.075
0.448ThrCys: 0.448 ± 0.021
3.858ThrAsp: 3.858 ± 0.053
2.828ThrGlu: 2.828 ± 0.049
1.935ThrPhe: 1.935 ± 0.044
6.25ThrGly: 6.25 ± 0.075
1.316ThrHis: 1.316 ± 0.029
2.481ThrIle: 2.481 ± 0.045
1.318ThrLys: 1.318 ± 0.033
5.85ThrLeu: 5.85 ± 0.061
1.157ThrMet: 1.157 ± 0.029
1.294ThrAsn: 1.294 ± 0.032
4.04ThrPro: 4.04 ± 0.061
1.551ThrGln: 1.551 ± 0.035
4.048ThrArg: 4.048 ± 0.056
4.001ThrSer: 4.001 ± 0.053
4.558ThrThr: 4.558 ± 0.075
6.188ThrVal: 6.188 ± 0.08
1.033ThrTrp: 1.033 ± 0.032
1.294ThrTyr: 1.294 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
12.558ValAla: 12.558 ± 0.118
0.711ValCys: 0.711 ± 0.024
6.038ValAsp: 6.038 ± 0.071
4.905ValGlu: 4.905 ± 0.064
2.545ValPhe: 2.545 ± 0.053
8.218ValGly: 8.218 ± 0.087
1.933ValHis: 1.933 ± 0.038
3.821ValIle: 3.821 ± 0.055
1.782ValLys: 1.782 ± 0.045
9.509ValLeu: 9.509 ± 0.098
1.639ValMet: 1.639 ± 0.037
1.778ValAsn: 1.778 ± 0.04
4.789ValPro: 4.789 ± 0.048
2.142ValGln: 2.142 ± 0.04
6.555ValArg: 6.555 ± 0.067
5.439ValSer: 5.439 ± 0.058
6.617ValThr: 6.617 ± 0.078
10.535ValVal: 10.535 ± 0.113
1.166ValTrp: 1.166 ± 0.032
1.423ValTyr: 1.423 ± 0.034
0.0ValXaa: 0.0 ± 0.0
Trp
1.798TrpAla: 1.798 ± 0.036
0.168TrpCys: 0.168 ± 0.01
0.722TrpAsp: 0.722 ± 0.025
0.574TrpGlu: 0.574 ± 0.022
0.593TrpPhe: 0.593 ± 0.02
1.146TrpGly: 1.146 ± 0.031
0.388TrpHis: 0.388 ± 0.017
0.511TrpIle: 0.511 ± 0.021
0.313TrpLys: 0.313 ± 0.015
1.862TrpLeu: 1.862 ± 0.038
0.302TrpMet: 0.302 ± 0.015
0.419TrpAsn: 0.419 ± 0.019
0.847TrpPro: 0.847 ± 0.026
0.586TrpGln: 0.586 ± 0.022
1.273TrpArg: 1.273 ± 0.029
1.216TrpSer: 1.216 ± 0.03
0.897TrpThr: 0.897 ± 0.03
1.35TrpVal: 1.35 ± 0.034
0.385TrpTrp: 0.385 ± 0.02
0.292TrpTyr: 0.292 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.504TyrAla: 2.504 ± 0.04
0.147TyrCys: 0.147 ± 0.011
1.32TyrAsp: 1.32 ± 0.033
0.995TyrGlu: 0.995 ± 0.025
0.624TyrPhe: 0.624 ± 0.022
1.785TyrGly: 1.785 ± 0.039
0.344TyrHis: 0.344 ± 0.016
0.496TyrIle: 0.496 ± 0.02
0.303TyrLys: 0.303 ± 0.015
2.137TyrLeu: 2.137 ± 0.039
0.2TyrMet: 0.2 ± 0.011
0.401TyrAsn: 0.401 ± 0.021
1.016TyrPro: 1.016 ± 0.027
0.551TyrGln: 0.551 ± 0.02
1.536TyrArg: 1.536 ± 0.034
0.957TyrSer: 0.957 ± 0.027
1.028TyrThr: 1.028 ± 0.029
1.682TyrVal: 1.682 ± 0.037
0.298TyrTrp: 0.298 ± 0.016
0.432TyrTyr: 0.432 ± 0.019
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4370 proteins (1425508 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski