Emiliania huxleyi (Pontosphaera huxleyi)

Taxonomy: cellular organisms; Eukaryota; Haptista; Haptophyta; Prymnesiophyceae; Isochrysidales; Noelaerhabdaceae; Emiliania

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35707 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R1F680|R1F680_EMIHU PEX-1N domain-containing protein OS=Emiliania huxleyi OX=2903 GN=EMIHUDRAFT_234582 PE=4 SV=1
MM1 pKa = 7.35MGLFHH6 pKa = 7.17NISYY10 pKa = 10.42FNEE13 pKa = 5.48DD14 pKa = 2.42ISTWDD19 pKa = 3.21TGAVTDD25 pKa = 4.47MEE27 pKa = 5.29SMFYY31 pKa = 10.87DD32 pKa = 3.25AVSFNQDD39 pKa = 3.86LPWDD43 pKa = 3.93TSSVEE48 pKa = 4.07TFKK51 pKa = 11.29QMFAQAVAFNGDD63 pKa = 2.59ISTWDD68 pKa = 5.2DD69 pKa = 2.78ISTWDD74 pKa = 3.53MSNVRR79 pKa = 11.84TTSAMFSGAFAFNQDD94 pKa = 2.25ISTWDD99 pKa = 3.29VHH101 pKa = 6.0NVRR104 pKa = 11.84DD105 pKa = 3.8MTSMFFASPFNQDD118 pKa = 2.05ITGWNVSNVEE128 pKa = 3.83FMNAMFASSNFNRR141 pKa = 11.84TAYY144 pKa = 9.67PLEE147 pKa = 4.42TTGEE151 pKa = 3.77WAEE154 pKa = 4.13RR155 pKa = 11.84LNTTAVVNATCPPQPPDD172 pKa = 4.02DD173 pKa = 3.75FWVISGLGANQQLYY187 pKa = 9.72CGHH190 pKa = 6.3SCCPLTGALRR200 pKa = 11.84PPP202 pKa = 3.86

Molecular weight: 22.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R1DUB5|R1DUB5_EMIHU Uncharacterized protein OS=Emiliania huxleyi OX=2903 GN=EMIHUDRAFT_461318 PE=3 SV=1
TT1 pKa = 7.3RR2 pKa = 11.84PVTRR6 pKa = 11.84PPAIHH11 pKa = 6.49RR12 pKa = 11.84TRR14 pKa = 11.84PVTRR18 pKa = 11.84PPAIHH23 pKa = 6.49RR24 pKa = 11.84TRR26 pKa = 11.84PVTRR30 pKa = 11.84PPAIHH35 pKa = 6.49RR36 pKa = 11.84TRR38 pKa = 11.84PVTRR42 pKa = 11.84PPAIHH47 pKa = 6.49RR48 pKa = 11.84TRR50 pKa = 11.84PVTRR54 pKa = 11.84PPP56 pKa = 3.23

Molecular weight: 6.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

35676

31

35707

12569600

25

21165

352.0

37.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.672 ± 0.033

1.986 ± 0.007

4.783 ± 0.009

6.162 ± 0.018

2.916 ± 0.008

8.95 ± 0.021

2.219 ± 0.006

2.585 ± 0.009

2.84 ± 0.012

10.07 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.803 ± 0.006

1.885 ± 0.008

6.414 ± 0.015

3.059 ± 0.008

7.806 ± 0.017

7.211 ± 0.012

4.638 ± 0.009

6.571 ± 0.012

1.511 ± 0.005

1.92 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski