Tortoise microvirus 28

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W6T0|A0A4P8W6T0_9VIRU Uncharacterized protein OS=Tortoise microvirus 28 OX=2583130 PE=4 SV=1
MM1 pKa = 7.77RR2 pKa = 11.84LLMEE6 pKa = 4.84AGEE9 pKa = 4.26RR10 pKa = 11.84LEE12 pKa = 3.69AHH14 pKa = 6.33YY15 pKa = 11.05RR16 pKa = 11.84NTYY19 pKa = 9.3PGLDD23 pKa = 3.25GLEE26 pKa = 4.23YY27 pKa = 10.65GSEE30 pKa = 4.13PEE32 pKa = 4.45EE33 pKa = 5.09DD34 pKa = 4.32DD35 pKa = 3.85PCSVTGYY42 pKa = 10.91GIDD45 pKa = 3.8EE46 pKa = 4.19MDD48 pKa = 4.43ALDD51 pKa = 3.67VMRR54 pKa = 11.84NAMYY58 pKa = 9.99RR59 pKa = 11.84HH60 pKa = 5.48EE61 pKa = 4.55AARR64 pKa = 11.84QEE66 pKa = 4.27EE67 pKa = 4.57LQVAASSPRR76 pKa = 11.84EE77 pKa = 4.02RR78 pKa = 11.84EE79 pKa = 3.97SEE81 pKa = 4.28LPTEE85 pKa = 4.81ASPKK89 pKa = 10.61GEE91 pKa = 4.1SEE93 pKa = 4.47TEE95 pKa = 3.88AEE97 pKa = 4.21

Molecular weight: 10.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W9E1|A0A4P8W9E1_9VIRU DNA pilot protein OS=Tortoise microvirus 28 OX=2583130 PE=4 SV=1
MM1 pKa = 7.74GLRR4 pKa = 11.84KK5 pKa = 8.72KK6 pKa = 9.18TGFFYY11 pKa = 10.8KK12 pKa = 10.5KK13 pKa = 9.46SCKK16 pKa = 8.94KK17 pKa = 10.7KK18 pKa = 8.64KK19 pKa = 7.98MCYY22 pKa = 8.92IIYY25 pKa = 8.36MAHH28 pKa = 5.61LQCRR32 pKa = 11.84KK33 pKa = 9.8RR34 pKa = 11.84VFTRR38 pKa = 11.84NGVFGCGHH46 pKa = 6.68CEE48 pKa = 3.7VCRR51 pKa = 11.84LKK53 pKa = 10.43QARR56 pKa = 11.84EE57 pKa = 3.5WSIRR61 pKa = 11.84MYY63 pKa = 10.72HH64 pKa = 5.6EE65 pKa = 4.69WKK67 pKa = 10.79SSDD70 pKa = 3.14HH71 pKa = 6.36KK72 pKa = 11.48ALFVLLTYY80 pKa = 11.01KK81 pKa = 10.85DD82 pKa = 3.61MPKK85 pKa = 10.42NGSLNFDD92 pKa = 3.95DD93 pKa = 4.64LHH95 pKa = 8.24NFFKK99 pKa = 10.98KK100 pKa = 9.71LRR102 pKa = 11.84KK103 pKa = 9.36RR104 pKa = 11.84FSRR107 pKa = 11.84QGRR110 pKa = 11.84KK111 pKa = 8.62FKK113 pKa = 10.55FYY115 pKa = 11.04ASGEE119 pKa = 4.09YY120 pKa = 10.52GEE122 pKa = 4.59RR123 pKa = 11.84RR124 pKa = 11.84TRR126 pKa = 11.84RR127 pKa = 11.84PHH129 pKa = 4.8FHH131 pKa = 6.73VILFGADD138 pKa = 2.72MADD141 pKa = 3.2RR142 pKa = 11.84KK143 pKa = 10.91DD144 pKa = 4.74IFEE147 pKa = 4.39CWGKK151 pKa = 10.41CLDD154 pKa = 3.48IPGCFGCRR162 pKa = 11.84VVNGRR167 pKa = 11.84KK168 pKa = 9.93AFMYY172 pKa = 8.74TAGYY176 pKa = 9.02CSKK179 pKa = 10.65KK180 pKa = 9.87IGSGYY185 pKa = 7.29TYY187 pKa = 10.81KK188 pKa = 10.2VRR190 pKa = 11.84KK191 pKa = 7.71MLKK194 pKa = 9.84KK195 pKa = 10.29EE196 pKa = 4.12PEE198 pKa = 3.85QSRR201 pKa = 11.84MSDD204 pKa = 3.67GIGKK208 pKa = 8.95DD209 pKa = 2.7WCLRR213 pKa = 11.84NLDD216 pKa = 4.67DD217 pKa = 5.0SPFMRR222 pKa = 11.84LDD224 pKa = 3.34GEE226 pKa = 4.87KK227 pKa = 9.77ILKK230 pKa = 8.19PRR232 pKa = 11.84YY233 pKa = 7.81YY234 pKa = 10.58RR235 pKa = 11.84KK236 pKa = 9.59ILSITADD243 pKa = 3.81DD244 pKa = 3.79FSEE247 pKa = 4.88FIKK250 pKa = 10.62DD251 pKa = 3.53YY252 pKa = 10.4EE253 pKa = 4.16EE254 pKa = 5.07KK255 pKa = 10.36IRR257 pKa = 11.84SDD259 pKa = 3.6VIGRR263 pKa = 11.84CPSAVLYY270 pKa = 10.54YY271 pKa = 10.71ADD273 pKa = 5.81DD274 pKa = 3.37IGGRR278 pKa = 11.84GFILTPDD285 pKa = 4.0YY286 pKa = 11.25YY287 pKa = 10.14RR288 pKa = 11.84TLNDD292 pKa = 4.72IGAQDD297 pKa = 4.48CLRR300 pKa = 11.84LFYY303 pKa = 10.57KK304 pKa = 10.27RR305 pKa = 11.84QDD307 pKa = 2.56WVARR311 pKa = 11.84KK312 pKa = 10.07GCII315 pKa = 3.68

Molecular weight: 37.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1326

97

570

265.2

30.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.089 ± 1.703

1.961 ± 0.989

5.053 ± 0.923

6.637 ± 2.095

4.751 ± 1.028

6.787 ± 0.496

2.79 ± 0.228

4.902 ± 0.538

4.374 ± 2.17

7.692 ± 0.526

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.941 ± 0.308

4.148 ± 0.935

5.958 ± 1.549

4.676 ± 1.146

8.296 ± 0.681

6.184 ± 0.396

4.751 ± 0.734

5.128 ± 0.708

1.282 ± 0.345

4.6 ± 0.521
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski