Aspergillus violaceofuscus (strain CBS 115571)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus; Aspergillus violaceofuscus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12074 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2V5HIV6|A0A2V5HIV6_ASPV1 GST N-terminal domain-containing protein OS=Aspergillus violaceofuscus (strain CBS 115571) OX=1450538 GN=BO99DRAFT_400523 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 10.23HH3 pKa = 6.08IDD5 pKa = 3.69KK6 pKa = 10.6LALAAACLPGMVLKK20 pKa = 10.5AAPLSEE26 pKa = 4.33DD27 pKa = 3.4TANAPSQAGEE37 pKa = 4.36TEE39 pKa = 4.21PVDD42 pKa = 3.55QGAEE46 pKa = 4.13STAEE50 pKa = 3.76ASAAAAAATPGFCDD64 pKa = 4.66CEE66 pKa = 4.47SSNCTDD72 pKa = 3.01VEE74 pKa = 4.24AGGRR78 pKa = 11.84GAGAGCDD85 pKa = 3.69GCPGGDD91 pKa = 3.12SVSPDD96 pKa = 2.62GRR98 pKa = 11.84GGYY101 pKa = 7.62NTLGNGTANSTGNGSGDD118 pKa = 3.86CADD121 pKa = 3.8VEE123 pKa = 4.35AGGRR127 pKa = 11.84GDD129 pKa = 3.96GNADD133 pKa = 3.59GTDD136 pKa = 4.07DD137 pKa = 4.71GCDD140 pKa = 3.45DD141 pKa = 4.23ANANAGSSDD150 pKa = 2.86GRR152 pKa = 11.84GGFRR156 pKa = 11.84LLCIGSPDD164 pKa = 4.12ADD166 pKa = 4.57PIATVDD172 pKa = 3.74LFF174 pKa = 4.09

Molecular weight:
16.6 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2V5HJT7|A0A2V5HJT7_ASPV1 Uncharacterized protein OS=Aspergillus violaceofuscus (strain CBS 115571) OX=1450538 GN=BO99DRAFT_321125 PE=4 SV=1
SS1 pKa = 6.85HH2 pKa = 7.5KK3 pKa = 10.71SFRR6 pKa = 11.84TKK8 pKa = 10.45QKK10 pKa = 9.54LAKK13 pKa = 9.74AQRR16 pKa = 11.84QNRR19 pKa = 11.84PIPQWIRR26 pKa = 11.84LRR28 pKa = 11.84TGNTIRR34 pKa = 11.84YY35 pKa = 5.79NAKK38 pKa = 8.89RR39 pKa = 11.84RR40 pKa = 11.84HH41 pKa = 4.14WRR43 pKa = 11.84KK44 pKa = 7.41TRR46 pKa = 11.84LGII49 pKa = 4.46

Molecular weight:
6.05 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12074

0

12074

5671855

49

7770

469.8

51.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.011 ± 0.021

1.326 ± 0.01

5.504 ± 0.016

5.975 ± 0.018

3.691 ± 0.015

6.847 ± 0.02

2.553 ± 0.01

4.726 ± 0.017

4.092 ± 0.017

9.311 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.09 ± 0.008

3.366 ± 0.012

6.125 ± 0.026

4.11 ± 0.018

6.302 ± 0.02

8.143 ± 0.025

6.154 ± 0.016

6.311 ± 0.018

1.517 ± 0.008

2.843 ± 0.012

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski