Amino acid dipepetide frequency for Escherichia phage Qbeta

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.087AlaAla: 4.087 ± 1.456
1.362AlaCys: 1.362 ± 0.879
2.725AlaAsp: 2.725 ± 1.988
3.406AlaGlu: 3.406 ± 1.478
2.725AlaPhe: 2.725 ± 1.717
2.725AlaGly: 2.725 ± 1.185
0.0AlaHis: 0.0 ± 0.0
5.45AlaIle: 5.45 ± 1.786
2.725AlaLys: 2.725 ± 0.649
12.262AlaLeu: 12.262 ± 3.249
0.0AlaMet: 0.0 ± 0.0
3.406AlaAsn: 3.406 ± 0.597
2.044AlaPro: 2.044 ± 0.999
0.681AlaGln: 0.681 ± 0.439
1.362AlaArg: 1.362 ± 0.879
2.725AlaSer: 2.725 ± 0.649
5.45AlaThr: 5.45 ± 1.525
6.812AlaVal: 6.812 ± 0.846
2.044AlaTrp: 2.044 ± 0.707
5.45AlaTyr: 5.45 ± 2.619
0.0AlaXaa: 0.0 ± 0.0
Cys
1.362CysAla: 1.362 ± 0.486
0.0CysCys: 0.0 ± 0.0
2.044CysAsp: 2.044 ± 0.943
0.681CysGlu: 0.681 ± 0.439
0.0CysPhe: 0.0 ± 0.0
0.681CysGly: 0.681 ± 0.439
0.0CysHis: 0.0 ± 0.0
2.044CysIle: 2.044 ± 1.318
0.0CysLys: 0.0 ± 0.0
0.0CysLeu: 0.0 ± 0.0
0.0CysMet: 0.0 ± 0.0
0.0CysAsn: 0.0 ± 0.0
1.362CysPro: 1.362 ± 1.371
0.0CysGln: 0.0 ± 0.0
0.681CysArg: 0.681 ± 0.439
2.725CysSer: 2.725 ± 1.174
4.087CysThr: 4.087 ± 0.864
0.0CysVal: 0.0 ± 0.0
0.0CysTrp: 0.0 ± 0.0
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
3.406AspAla: 3.406 ± 0.997
0.681AspCys: 0.681 ± 0.439
2.044AspAsp: 2.044 ± 0.421
2.725AspGlu: 2.725 ± 1.309
4.768AspPhe: 4.768 ± 1.703
7.493AspGly: 7.493 ± 1.526
0.0AspHis: 0.0 ± 0.0
6.812AspIle: 6.812 ± 1.917
1.362AspLys: 1.362 ± 0.486
6.812AspLeu: 6.812 ± 1.976
0.0AspMet: 0.0 ± 0.0
2.725AspAsn: 2.725 ± 1.185
5.45AspPro: 5.45 ± 2.436
1.362AspGln: 1.362 ± 0.879
1.362AspArg: 1.362 ± 0.879
6.812AspSer: 6.812 ± 1.963
1.362AspThr: 1.362 ± 0.486
5.45AspVal: 5.45 ± 1.786
3.406AspTrp: 3.406 ± 1.898
0.0AspTyr: 0.0 ± 0.0
0.0AspXaa: 0.0 ± 0.0
Glu
4.087GluAla: 4.087 ± 1.456
2.044GluCys: 2.044 ± 1.318
2.044GluAsp: 2.044 ± 0.707
2.044GluGlu: 2.044 ± 0.943
2.725GluPhe: 2.725 ± 0.829
3.406GluGly: 3.406 ± 0.597
0.0GluHis: 0.0 ± 0.0
2.044GluIle: 2.044 ± 0.999
4.087GluLys: 4.087 ± 1.705
8.174GluLeu: 8.174 ± 0.667
0.681GluMet: 0.681 ± 0.6
0.681GluAsn: 0.681 ± 0.439
2.044GluPro: 2.044 ± 0.83
0.681GluGln: 0.681 ± 0.6
3.406GluArg: 3.406 ± 1.313
2.044GluSer: 2.044 ± 0.707
1.362GluThr: 1.362 ± 0.486
2.044GluVal: 2.044 ± 0.999
0.681GluTrp: 0.681 ± 0.6
0.681GluTyr: 0.681 ± 0.686
0.0GluXaa: 0.0 ± 0.0
Phe
2.725PheAla: 2.725 ± 1.072
0.0PheCys: 0.0 ± 0.0
4.768PheAsp: 4.768 ± 1.9
1.362PheGlu: 1.362 ± 0.879
2.044PhePhe: 2.044 ± 0.707
2.044PheGly: 2.044 ± 0.421
0.681PheHis: 0.681 ± 0.6
2.044PheIle: 2.044 ± 1.799
2.044PheLys: 2.044 ± 0.707
1.362PheLeu: 1.362 ± 0.486
0.681PheMet: 0.681 ± 0.439
3.406PheAsn: 3.406 ± 1.478
0.681PhePro: 0.681 ± 0.439
1.362PheGln: 1.362 ± 0.486
6.812PheArg: 6.812 ± 1.195
8.174PheSer: 8.174 ± 1.322
2.044PheThr: 2.044 ± 0.943
0.681PheVal: 0.681 ± 0.686
0.681PheTrp: 0.681 ± 0.686
1.362PheTyr: 1.362 ± 0.486
0.0PheXaa: 0.0 ± 0.0
Gly
4.768GlyAla: 4.768 ± 1.826
0.0GlyCys: 0.0 ± 0.0
7.493GlyAsp: 7.493 ± 0.736
3.406GlyGlu: 3.406 ± 2.163
2.044GlyPhe: 2.044 ± 0.83
2.725GlyGly: 2.725 ± 0.404
2.044GlyHis: 2.044 ± 0.999
5.45GlyIle: 5.45 ± 0.89
3.406GlyLys: 3.406 ± 1.313
3.406GlyLeu: 3.406 ± 2.998
1.362GlyMet: 1.362 ± 0.486
3.406GlyAsn: 3.406 ± 0.876
2.725GlyPro: 2.725 ± 1.029
0.0GlyGln: 0.0 ± 0.0
2.044GlyArg: 2.044 ± 0.421
8.856GlySer: 8.856 ± 1.484
3.406GlyThr: 3.406 ± 0.997
7.493GlyVal: 7.493 ± 1.299
1.362GlyTrp: 1.362 ± 0.879
2.725GlyTyr: 2.725 ± 0.829
0.0GlyXaa: 0.0 ± 0.0
His
0.0HisAla: 0.0 ± 0.0
1.362HisCys: 1.362 ± 0.486
0.681HisAsp: 0.681 ± 0.6
1.362HisGlu: 1.362 ± 0.486
0.681HisPhe: 0.681 ± 0.6
1.362HisGly: 1.362 ± 1.199
0.0HisHis: 0.0 ± 0.0
0.0HisIle: 0.0 ± 0.0
0.681HisLys: 0.681 ± 0.6
0.681HisLeu: 0.681 ± 0.439
0.681HisMet: 0.681 ± 0.439
0.0HisAsn: 0.0 ± 0.0
0.681HisPro: 0.681 ± 0.439
0.0HisGln: 0.0 ± 0.0
2.044HisArg: 2.044 ± 0.707
1.362HisSer: 1.362 ± 1.371
0.681HisThr: 0.681 ± 0.6
0.681HisVal: 0.681 ± 0.6
0.0HisTrp: 0.0 ± 0.0
0.681HisTyr: 0.681 ± 0.439
0.0HisXaa: 0.0 ± 0.0
Ile
2.044IleAla: 2.044 ± 0.707
0.0IleCys: 0.0 ± 0.0
8.174IleAsp: 8.174 ± 1.304
2.725IleGlu: 2.725 ± 0.404
0.681IlePhe: 0.681 ± 0.439
3.406IleGly: 3.406 ± 0.82
0.681IleHis: 0.681 ± 0.439
4.087IleIle: 4.087 ± 1.458
2.044IleLys: 2.044 ± 1.799
3.406IleLeu: 3.406 ± 1.478
0.681IleMet: 0.681 ± 0.439
4.087IleAsn: 4.087 ± 1.127
3.406IlePro: 3.406 ± 1.384
2.725IleGln: 2.725 ± 1.299
3.406IleArg: 3.406 ± 1.453
5.45IleSer: 5.45 ± 1.618
5.45IleThr: 5.45 ± 0.879
6.131IleVal: 6.131 ± 1.461
0.681IleTrp: 0.681 ± 0.686
1.362IleTyr: 1.362 ± 1.371
0.0IleXaa: 0.0 ± 0.0
Lys
3.406LysAla: 3.406 ± 0.625
0.681LysCys: 0.681 ± 0.686
1.362LysAsp: 1.362 ± 0.486
1.362LysGlu: 1.362 ± 1.199
2.725LysPhe: 2.725 ± 1.174
1.362LysGly: 1.362 ± 1.199
1.362LysHis: 1.362 ± 0.486
4.087LysIle: 4.087 ± 0.571
2.044LysLys: 2.044 ± 1.318
4.768LysLeu: 4.768 ± 0.605
0.681LysMet: 0.681 ± 0.408
3.406LysAsn: 3.406 ± 1.887
0.681LysPro: 0.681 ± 0.6
0.681LysGln: 0.681 ± 0.6
2.725LysArg: 2.725 ± 1.299
2.044LysSer: 2.044 ± 0.421
2.725LysThr: 2.725 ± 1.758
2.725LysVal: 2.725 ± 1.084
0.0LysTrp: 0.0 ± 0.0
4.087LysTyr: 4.087 ± 1.127
0.0LysXaa: 0.0 ± 0.0
Leu
8.174LeuAla: 8.174 ± 1.86
0.681LeuCys: 0.681 ± 0.439
2.725LeuAsp: 2.725 ± 1.029
4.087LeuGlu: 4.087 ± 0.83
2.044LeuPhe: 2.044 ± 1.799
4.768LeuGly: 4.768 ± 1.985
1.362LeuHis: 1.362 ± 0.486
4.087LeuIle: 4.087 ± 0.864
4.768LeuLys: 4.768 ± 0.609
8.856LeuLeu: 8.856 ± 0.709
1.362LeuMet: 1.362 ± 1.188
6.131LeuAsn: 6.131 ± 2.828
3.406LeuPro: 3.406 ± 1.478
3.406LeuGln: 3.406 ± 1.359
8.856LeuArg: 8.856 ± 2.409
9.537LeuSer: 9.537 ± 1.874
4.087LeuThr: 4.087 ± 1.127
5.45LeuVal: 5.45 ± 1.11
2.725LeuTrp: 2.725 ± 0.972
3.406LeuTyr: 3.406 ± 1.131
0.0LeuXaa: 0.0 ± 0.0
Met
2.044MetAla: 2.044 ± 0.943
0.0MetCys: 0.0 ± 0.0
2.044MetAsp: 2.044 ± 0.999
0.681MetGlu: 0.681 ± 0.6
0.681MetPhe: 0.681 ± 0.439
2.044MetGly: 2.044 ± 0.707
0.0MetHis: 0.0 ± 0.0
0.681MetIle: 0.681 ± 0.439
0.0MetLys: 0.0 ± 0.0
2.044MetLeu: 2.044 ± 0.943
0.0MetMet: 0.0 ± 0.0
0.0MetAsn: 0.0 ± 0.0
0.681MetPro: 0.681 ± 0.6
0.681MetGln: 0.681 ± 0.686
1.362MetArg: 1.362 ± 1.199
1.362MetSer: 1.362 ± 0.879
0.0MetThr: 0.0 ± 0.0
0.0MetVal: 0.0 ± 0.0
0.0MetTrp: 0.0 ± 0.0
0.681MetTyr: 0.681 ± 0.439
0.0MetXaa: 0.0 ± 0.0
Asn
3.406AsnAla: 3.406 ± 1.898
0.0AsnCys: 0.0 ± 0.0
2.725AsnAsp: 2.725 ± 1.072
2.044AsnGlu: 2.044 ± 1.318
0.681AsnPhe: 0.681 ± 0.439
6.812AsnGly: 6.812 ± 1.501
0.0AsnHis: 0.0 ± 0.0
2.044AsnIle: 2.044 ± 0.999
2.044AsnLys: 2.044 ± 0.421
5.45AsnLeu: 5.45 ± 1.524
2.044AsnMet: 2.044 ± 0.707
0.681AsnAsn: 0.681 ± 0.6
6.812AsnPro: 6.812 ± 4.108
1.362AsnGln: 1.362 ± 0.879
4.087AsnArg: 4.087 ± 1.282
4.087AsnSer: 4.087 ± 1.138
2.044AsnThr: 2.044 ± 0.83
1.362AsnVal: 1.362 ± 0.486
0.0AsnTrp: 0.0 ± 0.0
2.044AsnTyr: 2.044 ± 0.943
0.0AsnXaa: 0.0 ± 0.0
Pro
4.768ProAla: 4.768 ± 1.524
1.362ProCys: 1.362 ± 0.486
3.406ProAsp: 3.406 ± 1.566
1.362ProGlu: 1.362 ± 1.199
6.131ProPhe: 6.131 ± 2.732
4.087ProGly: 4.087 ± 1.699
0.681ProHis: 0.681 ± 0.6
1.362ProIle: 1.362 ± 0.76
2.044ProLys: 2.044 ± 0.707
2.044ProLeu: 2.044 ± 1.184
2.044ProMet: 2.044 ± 0.943
2.044ProAsn: 2.044 ± 0.421
2.725ProPro: 2.725 ± 1.896
0.681ProGln: 0.681 ± 0.6
6.131ProArg: 6.131 ± 1.227
6.812ProSer: 6.812 ± 2.626
3.406ProThr: 3.406 ± 1.814
2.725ProVal: 2.725 ± 0.829
0.0ProTrp: 0.0 ± 0.0
2.044ProTyr: 2.044 ± 0.707
0.0ProXaa: 0.0 ± 0.0
Gln
3.406GlnAla: 3.406 ± 0.997
0.0GlnCys: 0.0 ± 0.0
0.681GlnAsp: 0.681 ± 0.686
1.362GlnGlu: 1.362 ± 0.486
0.681GlnPhe: 0.681 ± 0.439
0.0GlnGly: 0.0 ± 0.0
0.0GlnHis: 0.0 ± 0.0
0.0GlnIle: 0.0 ± 0.0
1.362GlnLys: 1.362 ± 1.199
1.362GlnLeu: 1.362 ± 0.879
0.681GlnMet: 0.681 ± 0.439
2.044GlnAsn: 2.044 ± 0.915
2.044GlnPro: 2.044 ± 0.943
1.362GlnGln: 1.362 ± 0.486
2.044GlnArg: 2.044 ± 1.799
0.681GlnSer: 0.681 ± 0.439
0.681GlnThr: 0.681 ± 0.439
2.044GlnVal: 2.044 ± 0.943
0.681GlnTrp: 0.681 ± 0.6
1.362GlnTyr: 1.362 ± 0.994
0.0GlnXaa: 0.0 ± 0.0
Arg
6.131ArgAla: 6.131 ± 0.902
0.681ArgCys: 0.681 ± 0.439
2.725ArgAsp: 2.725 ± 1.029
4.087ArgGlu: 4.087 ± 0.643
2.725ArgPhe: 2.725 ± 0.829
6.131ArgGly: 6.131 ± 0.918
4.087ArgHis: 4.087 ± 1.413
5.45ArgIle: 5.45 ± 1.525
4.087ArgLys: 4.087 ± 0.83
5.45ArgLeu: 5.45 ± 0.912
1.362ArgMet: 1.362 ± 1.098
3.406ArgAsn: 3.406 ± 0.625
2.725ArgPro: 2.725 ± 0.829
0.0ArgGln: 0.0 ± 0.0
8.174ArgArg: 8.174 ± 2.717
4.768ArgSer: 4.768 ± 0.605
2.725ArgThr: 2.725 ± 0.649
5.45ArgVal: 5.45 ± 1.618
2.044ArgTrp: 2.044 ± 0.999
2.044ArgTyr: 2.044 ± 1.184
0.0ArgXaa: 0.0 ± 0.0
Ser
3.406SerAla: 3.406 ± 0.625
2.725SerCys: 2.725 ± 1.084
6.131SerAsp: 6.131 ± 0.826
3.406SerGlu: 3.406 ± 1.313
5.45SerPhe: 5.45 ± 1.534
7.493SerGly: 7.493 ± 1.781
1.362SerHis: 1.362 ± 0.76
7.493SerIle: 7.493 ± 2.819
2.725SerLys: 2.725 ± 1.174
10.218SerLeu: 10.218 ± 2.21
0.681SerMet: 0.681 ± 0.439
2.725SerAsn: 2.725 ± 0.829
4.768SerPro: 4.768 ± 1.985
1.362SerGln: 1.362 ± 0.994
5.45SerArg: 5.45 ± 2.017
7.493SerSer: 7.493 ± 1.752
2.725SerThr: 2.725 ± 0.649
8.856SerVal: 8.856 ± 1.501
0.681SerTrp: 0.681 ± 0.686
4.087SerTyr: 4.087 ± 0.643
0.0SerXaa: 0.0 ± 0.0
Thr
4.087ThrAla: 4.087 ± 1.584
2.725ThrCys: 2.725 ± 1.309
3.406ThrAsp: 3.406 ± 1.313
2.044ThrGlu: 2.044 ± 0.943
4.768ThrPhe: 4.768 ± 0.609
1.362ThrGly: 1.362 ± 0.76
0.681ThrHis: 0.681 ± 0.6
3.406ThrIle: 3.406 ± 1.359
1.362ThrLys: 1.362 ± 0.879
5.45ThrLeu: 5.45 ± 1.81
0.0ThrMet: 0.0 ± 0.0
5.45ThrAsn: 5.45 ± 1.618
4.087ThrPro: 4.087 ± 1.998
2.044ThrGln: 2.044 ± 0.915
2.725ThrArg: 2.725 ± 0.649
5.45ThrSer: 5.45 ± 1.65
2.725ThrThr: 2.725 ± 0.404
3.406ThrVal: 3.406 ± 1.131
0.0ThrTrp: 0.0 ± 0.0
2.044ThrTyr: 2.044 ± 0.707
0.0ThrXaa: 0.0 ± 0.0
Val
2.725ValAla: 2.725 ± 0.649
0.681ValCys: 0.681 ± 0.439
3.406ValAsp: 3.406 ± 1.453
3.406ValGlu: 3.406 ± 0.732
0.681ValPhe: 0.681 ± 0.439
4.768ValGly: 4.768 ± 0.925
0.0ValHis: 0.0 ± 0.0
2.044ValIle: 2.044 ± 0.421
2.725ValLys: 2.725 ± 0.649
4.087ValLeu: 4.087 ± 1.899
1.362ValMet: 1.362 ± 1.199
1.362ValAsn: 1.362 ± 0.994
6.812ValPro: 6.812 ± 1.579
2.725ValGln: 2.725 ± 0.649
7.493ValArg: 7.493 ± 1.596
4.768ValSer: 4.768 ± 0.605
8.856ValThr: 8.856 ± 3.65
4.087ValVal: 4.087 ± 1.865
2.044ValTrp: 2.044 ± 0.707
3.406ValTyr: 3.406 ± 0.732
0.0ValXaa: 0.0 ± 0.0
Trp
0.681TrpAla: 0.681 ± 0.439
0.0TrpCys: 0.0 ± 0.0
3.406TrpAsp: 3.406 ± 1.697
1.362TrpGlu: 1.362 ± 0.486
1.362TrpPhe: 1.362 ± 0.486
0.681TrpGly: 0.681 ± 0.6
0.0TrpHis: 0.0 ± 0.0
0.681TrpIle: 0.681 ± 0.439
1.362TrpLys: 1.362 ± 1.199
0.681TrpLeu: 0.681 ± 0.6
0.0TrpMet: 0.0 ± 0.0
2.044TrpAsn: 2.044 ± 0.707
0.0TrpPro: 0.0 ± 0.0
0.0TrpGln: 0.0 ± 0.0
1.362TrpArg: 1.362 ± 1.371
0.681TrpSer: 0.681 ± 0.439
0.681TrpThr: 0.681 ± 0.686
0.681TrpVal: 0.681 ± 0.439
0.0TrpTrp: 0.0 ± 0.0
2.044TrpTyr: 2.044 ± 1.799
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.725TyrAla: 2.725 ± 1.185
0.681TyrCys: 0.681 ± 0.686
2.044TyrAsp: 2.044 ± 0.707
2.044TyrGlu: 2.044 ± 0.83
1.362TyrPhe: 1.362 ± 0.486
4.768TyrGly: 4.768 ± 1.591
0.681TyrHis: 0.681 ± 0.686
1.362TyrIle: 1.362 ± 0.622
2.044TyrLys: 2.044 ± 0.915
3.406TyrLeu: 3.406 ± 1.828
0.0TyrMet: 0.0 ± 0.769
2.725TyrAsn: 2.725 ± 0.829
2.725TyrPro: 2.725 ± 1.575
1.362TyrGln: 1.362 ± 1.199
2.725TyrArg: 2.725 ± 1.758
3.406TyrSer: 3.406 ± 0.82
2.725TyrThr: 2.725 ± 1.029
1.362TyrVal: 1.362 ± 0.879
0.681TyrTrp: 0.681 ± 0.686
0.0TyrTyr: 0.0 ± 0.0
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4 proteins (1469 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski