Amino acid dipepetide frequency for Paenibacillus aquistagni

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.433AlaAla: 8.433 ± 0.097	0.869AlaCys: 0.869 ± 0.026	4.072AlaAsp: 4.072 ± 0.051	5.342AlaGlu: 5.342 ± 0.075	3.357AlaPhe: 3.357 ± 0.049	6.097AlaGly: 6.097 ± 0.06	1.704AlaHis: 1.704 ± 0.035	5.693AlaIle: 5.693 ± 0.073	4.509AlaLys: 4.509 ± 0.056	8.402AlaLeu: 8.402 ± 0.074	2.607AlaMet: 2.607 ± 0.047	2.703AlaAsn: 2.703 ± 0.039	2.66AlaPro: 2.66 ± 0.053	2.812AlaGln: 2.812 ± 0.048	3.524AlaArg: 3.524 ± 0.047	5.419AlaSer: 5.419 ± 0.075	3.292AlaThr: 3.292 ± 0.051	6.137AlaVal: 6.137 ± 0.072	1.04AlaTrp: 1.04 ± 0.029	2.923AlaTyr: 2.923 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	0.585CysAla: 0.585 ± 0.021	0.136CysCys: 0.136 ± 0.011	0.459CysAsp: 0.459 ± 0.019	0.503CysGlu: 0.503 ± 0.02	0.36CysPhe: 0.36 ± 0.015	0.749CysGly: 0.749 ± 0.027	0.219CysHis: 0.219 ± 0.012	0.558CysIle: 0.558 ± 0.02	0.388CysLys: 0.388 ± 0.016	0.792CysLeu: 0.792 ± 0.025	0.271CysMet: 0.271 ± 0.013	0.278CysAsn: 0.278 ± 0.015	0.361CysPro: 0.361 ± 0.016	0.268CysGln: 0.268 ± 0.012	0.407CysArg: 0.407 ± 0.016	0.617CysSer: 0.617 ± 0.023	0.477CysThr: 0.477 ± 0.017	0.501CysVal: 0.501 ± 0.02	0.105CysTrp: 0.105 ± 0.009	0.307CysTyr: 0.307 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	4.015AspAla: 4.015 ± 0.056	0.41AspCys: 0.41 ± 0.019	2.376AspAsp: 2.376 ± 0.046	4.091AspGlu: 4.091 ± 0.057	1.79AspPhe: 1.79 ± 0.034	3.572AspGly: 3.572 ± 0.061	1.327AspHis: 1.327 ± 0.033	3.674AspIle: 3.674 ± 0.048	2.729AspLys: 2.729 ± 0.051	4.717AspLeu: 4.717 ± 0.055	1.635AspMet: 1.635 ± 0.034	1.666AspAsn: 1.666 ± 0.032	2.271AspPro: 2.271 ± 0.043	2.368AspGln: 2.368 ± 0.038	2.707AspArg: 2.707 ± 0.041	2.732AspSer: 2.732 ± 0.045	2.567AspThr: 2.567 ± 0.042	3.559AspVal: 3.559 ± 0.058	0.806AspTrp: 0.806 ± 0.027	2.107AspTyr: 2.107 ± 0.041	0.0AspXaa: 0.0 ± 0.0
Glu	6.613GluAla: 6.613 ± 0.073	0.438GluCys: 0.438 ± 0.016	3.433GluAsp: 3.433 ± 0.052	5.899GluGlu: 5.899 ± 0.074	1.885GluPhe: 1.885 ± 0.037	4.541GluGly: 4.541 ± 0.05	2.043GluHis: 2.043 ± 0.044	3.949GluIle: 3.949 ± 0.054	3.91GluLys: 3.91 ± 0.054	7.569GluLeu: 7.569 ± 0.081	2.138GluMet: 2.138 ± 0.037	2.067GluAsn: 2.067 ± 0.04	2.433GluPro: 2.433 ± 0.046	4.723GluGln: 4.723 ± 0.075	4.475GluArg: 4.475 ± 0.069	3.456GluSer: 3.456 ± 0.054	3.087GluThr: 3.087 ± 0.048	4.599GluVal: 4.599 ± 0.063	1.035GluTrp: 1.035 ± 0.03	2.006GluTyr: 2.006 ± 0.039	0.0GluXaa: 0.0 ± 0.0
Phe	2.948PheAla: 2.948 ± 0.047	0.374PheCys: 0.374 ± 0.016	2.231PheAsp: 2.231 ± 0.036	2.454PheGlu: 2.454 ± 0.043	1.727PhePhe: 1.727 ± 0.045	2.964PheGly: 2.964 ± 0.042	0.941PheHis: 0.941 ± 0.022	2.961PheIle: 2.961 ± 0.049	1.905PheLys: 1.905 ± 0.04	3.564PheLeu: 3.564 ± 0.054	1.227PheMet: 1.227 ± 0.027	1.592PheAsn: 1.592 ± 0.038	1.452PhePro: 1.452 ± 0.032	1.413PheGln: 1.413 ± 0.03	1.706PheArg: 1.706 ± 0.034	2.608PheSer: 2.608 ± 0.043	2.313PheThr: 2.313 ± 0.041	2.982PheVal: 2.982 ± 0.046	0.504PheTrp: 0.504 ± 0.018	1.464PheTyr: 1.464 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	5.298GlyAla: 5.298 ± 0.072	0.711GlyCys: 0.711 ± 0.026	3.129GlyAsp: 3.129 ± 0.05	4.381GlyGlu: 4.381 ± 0.057	3.083GlyPhe: 3.083 ± 0.048	4.809GlyGly: 4.809 ± 0.07	1.537GlyHis: 1.537 ± 0.03	5.361GlyIle: 5.361 ± 0.068	3.925GlyLys: 3.925 ± 0.057	6.804GlyLeu: 6.804 ± 0.07	2.483GlyMet: 2.483 ± 0.046	2.334GlyAsn: 2.334 ± 0.052	1.702GlyPro: 1.702 ± 0.031	2.649GlyGln: 2.649 ± 0.041	3.166GlyArg: 3.166 ± 0.048	4.472GlySer: 4.472 ± 0.057	4.102GlyThr: 4.102 ± 0.054	5.146GlyVal: 5.146 ± 0.06	0.913GlyTrp: 0.913 ± 0.028	2.927GlyTyr: 2.927 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	2.048HisAla: 2.048 ± 0.041	0.239HisCys: 0.239 ± 0.012	1.323HisAsp: 1.323 ± 0.033	1.754HisGlu: 1.754 ± 0.034	0.944HisPhe: 0.944 ± 0.032	1.701HisGly: 1.701 ± 0.035	0.838HisHis: 0.838 ± 0.025	1.709HisIle: 1.709 ± 0.034	1.008HisLys: 1.008 ± 0.028	2.401HisLeu: 2.401 ± 0.057	0.714HisMet: 0.714 ± 0.02	0.779HisAsn: 0.779 ± 0.021	1.296HisPro: 1.296 ± 0.032	1.1HisGln: 1.1 ± 0.029	1.298HisArg: 1.298 ± 0.035	1.374HisSer: 1.374 ± 0.031	1.32HisThr: 1.32 ± 0.031	1.748HisVal: 1.748 ± 0.041	0.335HisTrp: 0.335 ± 0.016	1.088HisTyr: 1.088 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	6.158IleAla: 6.158 ± 0.08	0.625IleCys: 0.625 ± 0.021	3.672IleAsp: 3.672 ± 0.055	4.875IleGlu: 4.875 ± 0.055	2.38IlePhe: 2.38 ± 0.046	5.401IleGly: 5.401 ± 0.063	1.823IleHis: 1.823 ± 0.034	4.52IleIle: 4.52 ± 0.066	3.038IleLys: 3.038 ± 0.053	5.868IleLeu: 5.868 ± 0.082	1.907IleMet: 1.907 ± 0.04	2.178IleAsn: 2.178 ± 0.04	3.158IlePro: 3.158 ± 0.045	2.958IleGln: 2.958 ± 0.041	3.589IleArg: 3.589 ± 0.052	4.2IleSer: 4.2 ± 0.058	3.81IleThr: 3.81 ± 0.056	5.345IleVal: 5.345 ± 0.066	0.741IleTrp: 0.741 ± 0.022	2.107IleTyr: 2.107 ± 0.043	0.0IleXaa: 0.0 ± 0.0
Lys	4.081LysAla: 4.081 ± 0.058	0.294LysCys: 0.294 ± 0.013	3.011LysAsp: 3.011 ± 0.047	4.56LysGlu: 4.56 ± 0.061	1.309LysPhe: 1.309 ± 0.035	3.41LysGly: 3.41 ± 0.055	1.481LysHis: 1.481 ± 0.029	2.795LysIle: 2.795 ± 0.051	3.381LysLys: 3.381 ± 0.059	5.643LysLeu: 5.643 ± 0.063	1.566LysMet: 1.566 ± 0.029	1.754LysAsn: 1.754 ± 0.037	2.32LysPro: 2.32 ± 0.036	3.071LysGln: 3.071 ± 0.043	3.196LysArg: 3.196 ± 0.046	2.853LysSer: 2.853 ± 0.048	2.642LysThr: 2.642 ± 0.038	3.534LysVal: 3.534 ± 0.055	0.736LysTrp: 0.736 ± 0.025	1.587LysTyr: 1.587 ± 0.034	0.0LysXaa: 0.0 ± 0.0
Leu	8.148LeuAla: 8.148 ± 0.083	0.875LeuCys: 0.875 ± 0.025	5.089LeuAsp: 5.089 ± 0.056	6.534LeuGlu: 6.534 ± 0.072	4.405LeuPhe: 4.405 ± 0.066	6.458LeuGly: 6.458 ± 0.075	2.544LeuHis: 2.544 ± 0.052	6.755LeuIle: 6.755 ± 0.094	5.246LeuLys: 5.246 ± 0.06	10.893LeuLeu: 10.893 ± 0.121	2.985LeuMet: 2.985 ± 0.051	3.849LeuAsn: 3.849 ± 0.054	4.349LeuPro: 4.349 ± 0.056	4.41LeuGln: 4.41 ± 0.059	4.971LeuArg: 4.971 ± 0.059	7.052LeuSer: 7.052 ± 0.07	5.521LeuThr: 5.521 ± 0.058	6.385LeuVal: 6.385 ± 0.067	1.052LeuTrp: 1.052 ± 0.029	3.33LeuTyr: 3.33 ± 0.046	0.0LeuXaa: 0.0 ± 0.0
Met	2.242MetAla: 2.242 ± 0.048	0.197MetCys: 0.197 ± 0.011	1.721MetAsp: 1.721 ± 0.033	2.191MetGlu: 2.191 ± 0.038	1.128MetPhe: 1.128 ± 0.024	1.754MetGly: 1.754 ± 0.034	0.632MetHis: 0.632 ± 0.017	2.252MetIle: 2.252 ± 0.045	2.315MetLys: 2.315 ± 0.038	3.429MetLeu: 3.429 ± 0.055	1.134MetMet: 1.134 ± 0.033	1.769MetAsn: 1.769 ± 0.034	1.186MetPro: 1.186 ± 0.034	1.22MetGln: 1.22 ± 0.027	1.435MetArg: 1.435 ± 0.032	2.045MetSer: 2.045 ± 0.035	1.882MetThr: 1.882 ± 0.032	1.907MetVal: 1.907 ± 0.041	0.264MetTrp: 0.264 ± 0.014	0.869MetTyr: 0.869 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	2.747AsnAla: 2.747 ± 0.047	0.246AsnCys: 0.246 ± 0.014	1.819AsnAsp: 1.819 ± 0.043	2.708AsnGlu: 2.708 ± 0.043	1.122AsnPhe: 1.122 ± 0.031	2.864AsnGly: 2.864 ± 0.055	1.003AsnHis: 1.003 ± 0.025	2.294AsnIle: 2.294 ± 0.046	2.042AsnLys: 2.042 ± 0.04	3.045AsnLeu: 3.045 ± 0.044	1.129AsnMet: 1.129 ± 0.027	1.395AsnAsn: 1.395 ± 0.033	1.816AsnPro: 1.816 ± 0.037	1.666AsnGln: 1.666 ± 0.037	2.01AsnArg: 2.01 ± 0.039	1.882AsnSer: 1.882 ± 0.038	1.888AsnThr: 1.888 ± 0.037	2.504AsnVal: 2.504 ± 0.044	0.514AsnTrp: 0.514 ± 0.018	1.205AsnTyr: 1.205 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	3.098ProAla: 3.098 ± 0.05	0.261ProCys: 0.261 ± 0.013	2.371ProAsp: 2.371 ± 0.04	3.11ProGlu: 3.11 ± 0.048	1.868ProPhe: 1.868 ± 0.035	2.24ProGly: 2.24 ± 0.039	1.02ProHis: 1.02 ± 0.025	2.759ProIle: 2.759 ± 0.042	1.855ProLys: 1.855 ± 0.035	3.875ProLeu: 3.875 ± 0.05	1.084ProMet: 1.084 ± 0.03	1.62ProAsn: 1.62 ± 0.035	1.153ProPro: 1.153 ± 0.032	1.32ProGln: 1.32 ± 0.029	1.405ProArg: 1.405 ± 0.03	2.777ProSer: 2.777 ± 0.052	2.006ProThr: 2.006 ± 0.037	2.89ProVal: 2.89 ± 0.045	0.538ProTrp: 0.538 ± 0.019	1.683ProTyr: 1.683 ± 0.04	0.0ProXaa: 0.0 ± 0.0
Gln	4.38GlnAla: 4.38 ± 0.056	0.283GlnCys: 0.283 ± 0.014	2.254GlnAsp: 2.254 ± 0.042	3.415GlnGlu: 3.415 ± 0.057	1.675GlnPhe: 1.675 ± 0.033	2.866GlnGly: 2.866 ± 0.043	1.203GlnHis: 1.203 ± 0.028	2.474GlnIle: 2.474 ± 0.043	1.825GlnLys: 1.825 ± 0.038	4.871GlnLeu: 4.871 ± 0.073	1.411GlnMet: 1.411 ± 0.027	1.137GlnAsn: 1.137 ± 0.028	1.533GlnPro: 1.533 ± 0.031	2.436GlnGln: 2.436 ± 0.044	1.995GlnArg: 1.995 ± 0.037	2.555GlnSer: 2.555 ± 0.047	1.79GlnThr: 1.79 ± 0.035	3.002GlnVal: 3.002 ± 0.049	0.675GlnTrp: 0.675 ± 0.023	1.628GlnTyr: 1.628 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	3.508ArgAla: 3.508 ± 0.048	0.372ArgCys: 0.372 ± 0.018	2.37ArgAsp: 2.37 ± 0.04	3.648ArgGlu: 3.648 ± 0.057	2.063ArgPhe: 2.063 ± 0.038	2.846ArgGly: 2.846 ± 0.049	1.157ArgHis: 1.157 ± 0.027	3.793ArgIle: 3.793 ± 0.051	2.822ArgLys: 2.822 ± 0.051	5.213ArgLeu: 5.213 ± 0.07	1.844ArgMet: 1.844 ± 0.038	1.771ArgAsn: 1.771 ± 0.037	1.682ArgPro: 1.682 ± 0.04	2.073ArgGln: 2.073 ± 0.038	2.509ArgArg: 2.509 ± 0.043	3.159ArgSer: 3.159 ± 0.043	2.677ArgThr: 2.677 ± 0.053	3.25ArgVal: 3.25 ± 0.052	0.688ArgTrp: 0.688 ± 0.021	2.096ArgTyr: 2.096 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	4.427SerAla: 4.427 ± 0.06	0.508SerCys: 0.508 ± 0.02	2.975SerAsp: 2.975 ± 0.05	3.738SerGlu: 3.738 ± 0.059	3.049SerPhe: 3.049 ± 0.05	4.59SerGly: 4.59 ± 0.062	1.455SerHis: 1.455 ± 0.029	4.713SerIle: 4.713 ± 0.063	3.401SerLys: 3.401 ± 0.053	6.381SerLeu: 6.381 ± 0.065	2.145SerMet: 2.145 ± 0.04	2.376SerAsn: 2.376 ± 0.045	2.27SerPro: 2.27 ± 0.039	2.286SerGln: 2.286 ± 0.04	2.927SerArg: 2.927 ± 0.042	4.67SerSer: 4.67 ± 0.075	3.461SerThr: 3.461 ± 0.052	4.128SerVal: 4.128 ± 0.057	0.831SerTrp: 0.831 ± 0.026	2.429SerTyr: 2.429 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	4.411ThrAla: 4.411 ± 0.057	0.425ThrCys: 0.425 ± 0.017	2.544ThrAsp: 2.544 ± 0.048	3.208ThrGlu: 3.208 ± 0.052	2.254ThrPhe: 2.254 ± 0.039	4.086ThrGly: 4.086 ± 0.057	1.113ThrHis: 1.113 ± 0.029	3.915ThrIle: 3.915 ± 0.054	2.624ThrLys: 2.624 ± 0.048	5.459ThrLeu: 5.459 ± 0.068	1.531ThrMet: 1.531 ± 0.032	1.957ThrAsn: 1.957 ± 0.04	2.414ThrPro: 2.414 ± 0.042	1.623ThrGln: 1.623 ± 0.033	2.128ThrArg: 2.128 ± 0.037	3.41ThrSer: 3.41 ± 0.059	2.862ThrThr: 2.862 ± 0.052	3.821ThrVal: 3.821 ± 0.052	0.674ThrTrp: 0.674 ± 0.023	1.9ThrTyr: 1.9 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	4.941ValAla: 4.941 ± 0.058	0.624ValCys: 0.624 ± 0.02	3.665ValAsp: 3.665 ± 0.048	4.613ValGlu: 4.613 ± 0.057	2.796ValPhe: 2.796 ± 0.043	4.446ValGly: 4.446 ± 0.064	1.778ValHis: 1.778 ± 0.032	5.056ValIle: 5.056 ± 0.062	3.883ValLys: 3.883 ± 0.053	7.2ValLeu: 7.2 ± 0.081	2.177ValMet: 2.177 ± 0.046	2.677ValAsn: 2.677 ± 0.045	2.946ValPro: 2.946 ± 0.055	2.927ValGln: 2.927 ± 0.045	3.419ValArg: 3.419 ± 0.05	4.512ValSer: 4.512 ± 0.06	3.993ValThr: 3.993 ± 0.061	5.061ValVal: 5.061 ± 0.06	0.787ValTrp: 0.787 ± 0.022	2.292ValTyr: 2.292 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	0.744TrpAla: 0.744 ± 0.023	0.114TrpCys: 0.114 ± 0.008	0.646TrpAsp: 0.646 ± 0.021	0.806TrpGlu: 0.806 ± 0.024	0.588TrpPhe: 0.588 ± 0.018	0.829TrpGly: 0.829 ± 0.024	0.308TrpHis: 0.308 ± 0.014	0.984TrpIle: 0.984 ± 0.025	0.765TrpLys: 0.765 ± 0.02	1.493TrpLeu: 1.493 ± 0.034	0.516TrpMet: 0.516 ± 0.017	0.694TrpAsn: 0.694 ± 0.022	0.356TrpPro: 0.356 ± 0.017	0.527TrpGln: 0.527 ± 0.024	0.591TrpArg: 0.591 ± 0.022	0.857TrpSer: 0.857 ± 0.03	0.697TrpThr: 0.697 ± 0.02	0.806TrpVal: 0.806 ± 0.024	0.2TrpTrp: 0.2 ± 0.011	0.394TrpTyr: 0.394 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.772TyrAla: 2.772 ± 0.045	0.339TyrCys: 0.339 ± 0.017	1.961TyrAsp: 1.961 ± 0.042	2.469TyrGlu: 2.469 ± 0.045	1.547TyrPhe: 1.547 ± 0.033	2.707TyrGly: 2.707 ± 0.043	0.892TyrHis: 0.892 ± 0.027	2.136TyrIle: 2.136 ± 0.038	1.654TyrLys: 1.654 ± 0.036	3.233TyrLeu: 3.233 ± 0.045	1.067TyrMet: 1.067 ± 0.028	1.306TyrAsn: 1.306 ± 0.028	1.575TyrPro: 1.575 ± 0.036	1.554TyrGln: 1.554 ± 0.036	2.082TyrArg: 2.082 ± 0.042	2.107TyrSer: 2.107 ± 0.044	1.972TyrThr: 1.972 ± 0.036	2.489TyrVal: 2.489 ± 0.04	0.476TyrTrp: 0.476 ± 0.02	1.422TyrTyr: 1.422 ± 0.032	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4778 proteins (1524220 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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