Amazona aestiva (Blue-fronted Amazon parrot)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria;

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16092 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q3LXV2|A0A0Q3LXV2_AMAAE Selenoprotein P OS=Amazona aestiva OX=12930 GN=AAES_247708 PE=4 SV=1
MM1 pKa = 7.05MSEE4 pKa = 4.22AGEE7 pKa = 4.25EE8 pKa = 4.34SSSSSDD14 pKa = 3.18AEE16 pKa = 4.26SSVEE20 pKa = 3.93EE21 pKa = 4.29PSICGIVPTVYY32 pKa = 10.68SQDD35 pKa = 3.48EE36 pKa = 4.45GSTDD40 pKa = 3.52VTASPAFQCLDD51 pKa = 4.28EE52 pKa = 6.1IDD54 pKa = 4.29EE55 pKa = 4.62LLISDD60 pKa = 3.8SRR62 pKa = 11.84KK63 pKa = 10.35DD64 pKa = 3.46VNQNSGSTGNTEE76 pKa = 3.54QSLNVYY82 pKa = 9.7HH83 pKa = 7.25AMNNTVTEE91 pKa = 4.49TSQANVAFADD101 pKa = 3.57LMLIHH106 pKa = 6.93VDD108 pKa = 3.52PLAVRR113 pKa = 11.84TLKK116 pKa = 10.32SWNDD120 pKa = 3.17AYY122 pKa = 10.98LQCLNVAA129 pKa = 4.46

Molecular weight: 13.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q3PC69|A0A0Q3PC69_AMAAE Single-stranded DNA-binding protein OS=Amazona aestiva OX=12930 GN=AAES_114409 PE=3 SV=1
MM1 pKa = 7.46SLLGTTPVAVPWAGTSLHH19 pKa = 6.48SLGTXGDD26 pKa = 4.83LSPHH30 pKa = 6.0TGSXGSKK37 pKa = 10.3PEE39 pKa = 4.26GKK41 pKa = 10.44GSWGGRR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84RR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84KK68 pKa = 7.87GRR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 11.84RR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 11.84RR76 pKa = 11.84RR77 pKa = 11.84RR78 pKa = 11.84RR79 pKa = 11.84RR80 pKa = 11.84RR81 pKa = 11.84KK82 pKa = 8.32RR83 pKa = 11.84RR84 pKa = 11.84RR85 pKa = 11.84RR86 pKa = 11.84KK87 pKa = 8.65RR88 pKa = 11.84KK89 pKa = 7.6IRR91 pKa = 11.84RR92 pKa = 11.84RR93 pKa = 11.84RR94 pKa = 11.84RR95 pKa = 11.84WRR97 pKa = 11.84KK98 pKa = 7.38RR99 pKa = 11.84RR100 pKa = 11.84RR101 pKa = 11.84RR102 pKa = 11.84RR103 pKa = 11.84RR104 pKa = 11.84RR105 pKa = 11.84RR106 pKa = 11.84RR107 pKa = 11.84RR108 pKa = 11.84RR109 pKa = 11.84RR110 pKa = 11.84RR111 pKa = 11.84RR112 pKa = 11.84RR113 pKa = 11.84RR114 pKa = 11.84RR115 pKa = 11.84RR116 pKa = 11.84RR117 pKa = 11.84RR118 pKa = 11.84RR119 pKa = 11.84RR120 pKa = 11.84RR121 pKa = 11.84RR122 pKa = 11.84KK123 pKa = 7.87GRR125 pKa = 11.84RR126 pKa = 11.84RR127 pKa = 11.84RR128 pKa = 11.84RR129 pKa = 11.84RR130 pKa = 11.84RR131 pKa = 11.84RR132 pKa = 11.84RR133 pKa = 11.84RR134 pKa = 11.84RR135 pKa = 11.84RR136 pKa = 11.84RR137 pKa = 11.84RR138 pKa = 11.84RR139 pKa = 11.84RR140 pKa = 11.84RR141 pKa = 11.84RR142 pKa = 11.84RR143 pKa = 11.84RR144 pKa = 11.84RR145 pKa = 11.84KK146 pKa = 10.05GGGKK150 pKa = 10.37GEE152 pKa = 4.14GKK154 pKa = 9.48EE155 pKa = 3.98RR156 pKa = 11.84QRR158 pKa = 11.84KK159 pKa = 7.12RR160 pKa = 11.84RR161 pKa = 11.84KK162 pKa = 9.64RR163 pKa = 11.84RR164 pKa = 11.84WKK166 pKa = 9.05RR167 pKa = 11.84RR168 pKa = 11.84GRR170 pKa = 11.84EE171 pKa = 3.79GSGEE175 pKa = 3.93GTEE178 pKa = 4.54EE179 pKa = 3.8GRR181 pKa = 11.84AMGRR185 pKa = 11.84AMGRR189 pKa = 11.84AEE191 pKa = 4.56LRR193 pKa = 11.84GEE195 pKa = 4.04

Molecular weight: 25.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16092

0

16092

8336878

66

7549

518.1

57.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.843 ± 0.018

2.216 ± 0.014

4.957 ± 0.012

7.297 ± 0.027

3.657 ± 0.013

6.141 ± 0.025

2.502 ± 0.009

4.656 ± 0.015

6.167 ± 0.023

9.688 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.331 ± 0.009

3.873 ± 0.013

5.525 ± 0.023

4.688 ± 0.015

5.315 ± 0.016

8.25 ± 0.023

5.274 ± 0.014

6.186 ± 0.015

1.185 ± 0.007

2.812 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski