Mycoplasma sp. (ex Biomphalaria glabrata)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; unclassified Mycoplasma

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 555 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U3MRP5|A0A0U3MRP5_9MOLU YqgFc domain-containing protein OS=Mycoplasma sp. (ex Biomphalaria glabrata) OX=1749074 GN=ASO20_00455 PE=4 SV=1
MM1 pKa = 7.22NKK3 pKa = 9.66KK4 pKa = 10.13FKK6 pKa = 10.83LLALLATLTTVTLVPISSFMNRR28 pKa = 11.84NTNEE32 pKa = 3.61QQISKK37 pKa = 9.4NHH39 pKa = 5.27SQQTQTTSQTFNSTTQQVDD58 pKa = 3.7YY59 pKa = 10.33ASKK62 pKa = 10.61SIKK65 pKa = 9.9EE66 pKa = 3.96FNANVSPFLNQFEE79 pKa = 4.61FFLKK83 pKa = 10.55NGLYY87 pKa = 10.25FVDD90 pKa = 4.05LTSAYY95 pKa = 9.32EE96 pKa = 4.08NQATPSEE103 pKa = 4.26KK104 pKa = 10.53DD105 pKa = 3.47AIDD108 pKa = 5.8KK109 pKa = 10.51ISLWIGKK116 pKa = 9.78SSDD119 pKa = 3.3YY120 pKa = 10.72RR121 pKa = 11.84FSPMTNEE128 pKa = 3.93QLVAAATANFPEE140 pKa = 4.91DD141 pKa = 4.01LNPDD145 pKa = 3.48EE146 pKa = 5.03LATVIDD152 pKa = 4.31NFASIQSIAAKK163 pKa = 9.33TLIDD167 pKa = 3.56LTYY170 pKa = 10.61SKK172 pKa = 9.91WIQTMEE178 pKa = 4.02GTEE181 pKa = 5.63DD182 pKa = 3.05GHH184 pKa = 6.2QWGWYY189 pKa = 7.24NQNNLSNMFPAYY201 pKa = 10.69VIDD204 pKa = 4.15SKK206 pKa = 11.81NGLTFDD212 pKa = 4.69NKK214 pKa = 10.8DD215 pKa = 3.77DD216 pKa = 5.08NIANPGVGLQNLLAKK231 pKa = 10.6FHH233 pKa = 7.5DD234 pKa = 4.27SLEE237 pKa = 4.37AGNPEE242 pKa = 4.73RR243 pKa = 11.84LTEE246 pKa = 4.43PYY248 pKa = 10.06LNPVSGSAIEE258 pKa = 4.33TAFHH262 pKa = 7.66EE263 pKa = 4.46YY264 pKa = 8.07QTWGKK269 pKa = 8.85NQFSYY274 pKa = 11.06YY275 pKa = 7.43VQKK278 pKa = 10.71IINQYY283 pKa = 9.33NSFYY287 pKa = 11.04YY288 pKa = 10.8GFVDD292 pKa = 5.76LNSQNQQYY300 pKa = 8.82WNYY303 pKa = 9.7YY304 pKa = 10.12LDD306 pKa = 5.09DD307 pKa = 3.88ATLNPLTSVDD317 pKa = 3.69EE318 pKa = 5.04VISKK322 pKa = 8.81WINVMKK328 pKa = 10.52DD329 pKa = 3.4PSWNASTVINFLFSTDD345 pKa = 2.58VWAGLSVDD353 pKa = 3.84SVGGKK358 pKa = 9.8DD359 pKa = 3.38ATIEE363 pKa = 3.92QYY365 pKa = 11.29EE366 pKa = 4.43FVATADD372 pKa = 3.81FNNFTLINTNSFKK385 pKa = 11.01LATNTLYY392 pKa = 11.11NQFLEE397 pKa = 4.24NNYY400 pKa = 10.1FGYY403 pKa = 10.97ADD405 pKa = 3.76PNGVFNVSQYY415 pKa = 10.9FSGTPTQIVTMQEE428 pKa = 4.08FKK430 pKa = 11.2DD431 pKa = 4.07SFIHH435 pKa = 5.86TAATYY440 pKa = 10.22IEE442 pKa = 4.07LSKK445 pKa = 11.13YY446 pKa = 9.68FIWTSEE452 pKa = 3.43GSYY455 pKa = 10.51YY456 pKa = 10.51RR457 pKa = 11.84PAEE460 pKa = 3.88NQNFLNYY467 pKa = 9.84VFYY470 pKa = 10.56QQSSLEE476 pKa = 3.86QDD478 pKa = 3.48FQSIIDD484 pKa = 4.15LVSPSYY490 pKa = 10.86YY491 pKa = 10.68GYY493 pKa = 11.45ASLANNRR500 pKa = 11.84LTTYY504 pKa = 10.16IFQNLEE510 pKa = 3.66VEE512 pKa = 4.59KK513 pKa = 10.98QMSAADD519 pKa = 3.6LADD522 pKa = 3.78YY523 pKa = 10.04FILNHH528 pKa = 6.82PATSDD533 pKa = 3.25EE534 pKa = 4.27YY535 pKa = 11.3LGVWATKK542 pKa = 11.08SMIDD546 pKa = 3.55SLKK549 pKa = 10.96SNPEE553 pKa = 3.68LGLNDD558 pKa = 3.52WVNDD562 pKa = 3.9QKK564 pKa = 9.85TQLNNDD570 pKa = 3.52LQKK573 pKa = 10.78IIDD576 pKa = 4.44KK577 pKa = 10.95IKK579 pKa = 10.46DD580 pKa = 3.44NYY582 pKa = 9.66FGWVTTTSGFGLLVSNVDD600 pKa = 3.28KK601 pKa = 11.21LLVNFPVNTEE611 pKa = 4.39LSPEE615 pKa = 4.49AIAATITMEE624 pKa = 4.21SLPTGLTGLYY634 pKa = 9.33EE635 pKa = 3.96IKK637 pKa = 10.82GFLKK641 pKa = 11.02LITSDD646 pKa = 5.5DD647 pKa = 3.45INTFKK652 pKa = 11.26NNEE655 pKa = 4.08SKK657 pKa = 11.08YY658 pKa = 10.06LSQEE662 pKa = 3.07ISRR665 pKa = 11.84IVPLFEE671 pKa = 3.56NTYY674 pKa = 8.89YY675 pKa = 10.83GYY677 pKa = 10.32SASPEE682 pKa = 3.86FGANAIYY689 pKa = 10.1QVIFYY694 pKa = 7.21THH696 pKa = 6.69RR697 pKa = 11.84TNTPPNEE704 pKa = 4.01QMIVDD709 pKa = 4.41AFKK712 pKa = 10.87QGLSVSAEE720 pKa = 4.16GFPKK724 pKa = 10.29PYY726 pKa = 10.21LVRR729 pKa = 11.84QFLGNITIEE738 pKa = 4.21NTDD741 pKa = 3.64FQAFVQDD748 pKa = 3.26QRR750 pKa = 11.84KK751 pKa = 8.21QVIDD755 pKa = 3.75HH756 pKa = 6.35FTTIIGMIEE765 pKa = 3.78PYY767 pKa = 10.4YY768 pKa = 10.84YY769 pKa = 10.93GFEE772 pKa = 3.83RR773 pKa = 11.84LANNSVSDD781 pKa = 4.08YY782 pKa = 11.46VLLGYY787 pKa = 9.2TDD789 pKa = 4.19GTPLDD794 pKa = 3.94PVVLANYY801 pKa = 10.58LMDD804 pKa = 4.79NFPSTPTIDD813 pKa = 5.92LPGKK817 pKa = 8.79WYY819 pKa = 11.06LNGFLTTLITDD830 pKa = 3.89KK831 pKa = 10.77TAINEE836 pKa = 4.06FLTDD840 pKa = 3.84QSNSLQKK847 pKa = 10.55AWNPIYY853 pKa = 10.78DD854 pKa = 3.66EE855 pKa = 4.42MVNNYY860 pKa = 9.72YY861 pKa = 10.88GYY863 pKa = 7.02TTEE866 pKa = 4.03MDD868 pKa = 3.71SLVSRR873 pKa = 11.84TLLIIQNDD881 pKa = 4.29GTPLDD886 pKa = 3.94SVALLKK892 pKa = 10.76LFKK895 pKa = 10.91QNVIDD900 pKa = 3.56MHH902 pKa = 6.05GTQDD906 pKa = 3.34FPFSYY911 pKa = 10.21GVKK914 pKa = 9.53VWLEE918 pKa = 3.82TLVNEE923 pKa = 4.42NKK925 pKa = 10.02ISGEE929 pKa = 3.92FKK931 pKa = 10.71DD932 pKa = 3.98EE933 pKa = 3.69QRR935 pKa = 11.84TALSYY940 pKa = 10.7DD941 pKa = 3.05IEE943 pKa = 4.52HH944 pKa = 7.41AVRR947 pKa = 11.84EE948 pKa = 4.26YY949 pKa = 11.43NNAYY953 pKa = 8.81WGYY956 pKa = 11.09NNDD959 pKa = 3.46TTLPNYY965 pKa = 10.15FIEE968 pKa = 4.44YY969 pKa = 10.4FLGSDD974 pKa = 3.52HH975 pKa = 6.61KK976 pKa = 9.92TPLTNAQIEE985 pKa = 4.77SNILDD990 pKa = 3.86AAKK993 pKa = 10.16GWGFYY998 pKa = 10.33KK999 pKa = 10.21IYY1001 pKa = 10.88DD1002 pKa = 3.93FFNSGISGGVFYY1014 pKa = 11.02DD1015 pKa = 3.37LFIFSEE1021 pKa = 4.28SQEE1024 pKa = 4.26VIAKK1028 pKa = 10.15GDD1030 pKa = 3.5LRR1032 pKa = 11.84NILEE1036 pKa = 5.07LLVQYY1041 pKa = 11.37YY1042 pKa = 10.33NDD1044 pKa = 3.49KK1045 pKa = 11.05DD1046 pKa = 3.81FNYY1049 pKa = 8.78TQTPSPFTGANHH1061 pKa = 6.48IWTLFICKK1069 pKa = 10.06NGSTYY1074 pKa = 11.07GLEE1077 pKa = 4.24SNVDD1081 pKa = 3.3GLYY1084 pKa = 10.19TLFKK1088 pKa = 10.78AQIAQTDD1095 pKa = 4.81LINRR1099 pKa = 11.84ITHH1102 pKa = 5.3YY1103 pKa = 10.86SVVAEE1108 pKa = 4.15YY1109 pKa = 10.84LKK1111 pKa = 10.84SLYY1114 pKa = 11.13VNDD1117 pKa = 4.78KK1118 pKa = 9.33GTLSAAGEE1126 pKa = 4.04LLNWKK1131 pKa = 8.55EE1132 pKa = 4.03WEE1134 pKa = 4.34GQQ1136 pKa = 3.33

Molecular weight: 129.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U3P426|A0A0U3P426_9MOLU DNA topoisomerase 1 OS=Mycoplasma sp. (ex Biomphalaria glabrata) OX=1749074 GN=topA PE=3 SV=1
MM1 pKa = 7.47LGKK4 pKa = 10.29NITNQRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.6LRR15 pKa = 11.84LRR17 pKa = 11.84KK18 pKa = 9.49RR19 pKa = 11.84LIGTSNQPRR28 pKa = 11.84LCVTVSNKK36 pKa = 10.26YY37 pKa = 10.05ISAQIIDD44 pKa = 4.64DD45 pKa = 4.41SNHH48 pKa = 5.35HH49 pKa = 5.98TLAFATTQTMEE60 pKa = 4.55LANKK64 pKa = 9.94NNIEE68 pKa = 4.01AATKK72 pKa = 10.38LGEE75 pKa = 4.18VLANRR80 pKa = 11.84AKK82 pKa = 10.26EE83 pKa = 3.99KK84 pKa = 10.68NITSIIFDD92 pKa = 3.57RR93 pKa = 11.84AGRR96 pKa = 11.84KK97 pKa = 7.37YY98 pKa = 10.09HH99 pKa = 6.58GKK101 pKa = 9.03VQAFADD107 pKa = 3.6ACRR110 pKa = 11.84ANGLIFF116 pKa = 4.37

Molecular weight: 13.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

555

0

555

197015

37

3066

355.0

40.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.957 ± 0.086

0.897 ± 0.038

5.24 ± 0.095

6.11 ± 0.12

5.479 ± 0.1

5.233 ± 0.084

1.772 ± 0.052

10.132 ± 0.14

8.864 ± 0.131

9.258 ± 0.084

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.945 ± 0.044

7.919 ± 0.13

2.911 ± 0.035

3.302 ± 0.064

2.865 ± 0.069

6.837 ± 0.107

5.572 ± 0.118

5.501 ± 0.08

1.077 ± 0.047

4.125 ± 0.083
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski