Amino acid dipepetide frequency for Staphylococcus phage vB_SauM_Romulus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.029AlaAla: 0.029 ± 0.029
0.5AlaCys: 0.5 ± 0.104
2.179AlaAsp: 2.179 ± 0.322
2.856AlaGlu: 2.856 ± 0.344
1.501AlaPhe: 1.501 ± 0.235
1.796AlaGly: 1.796 ± 0.296
1.06AlaHis: 1.06 ± 0.159
3.68AlaIle: 3.68 ± 0.407
4.416AlaLys: 4.416 ± 0.411
3.091AlaLeu: 3.091 ± 0.344
1.119AlaMet: 1.119 ± 0.219
2.473AlaAsn: 2.473 ± 0.322
1.295AlaPro: 1.295 ± 0.213
1.56AlaGln: 1.56 ± 0.275
1.384AlaArg: 1.384 ± 0.174
3.356AlaSer: 3.356 ± 0.414
2.797AlaThr: 2.797 ± 0.274
2.296AlaVal: 2.296 ± 0.278
0.383AlaTrp: 0.383 ± 0.108
2.09AlaTyr: 2.09 ± 0.234
0.0AlaXaa: 0.0 ± 0.0
Cys
0.265CysAla: 0.265 ± 0.073
0.088CysCys: 0.088 ± 0.049
0.294CysAsp: 0.294 ± 0.1
0.442CysGlu: 0.442 ± 0.126
0.353CysPhe: 0.353 ± 0.119
0.559CysGly: 0.559 ± 0.151
0.118CysHis: 0.118 ± 0.053
0.383CysIle: 0.383 ± 0.099
0.972CysLys: 0.972 ± 0.191
0.824CysLeu: 0.824 ± 0.169
0.147CysMet: 0.147 ± 0.06
0.471CysAsn: 0.471 ± 0.134
0.236CysPro: 0.236 ± 0.095
0.147CysGln: 0.147 ± 0.068
0.294CysArg: 0.294 ± 0.085
0.53CysSer: 0.53 ± 0.147
0.177CysThr: 0.177 ± 0.067
0.442CysVal: 0.442 ± 0.117
0.118CysTrp: 0.118 ± 0.049
0.677CysTyr: 0.677 ± 0.143
0.0CysXaa: 0.0 ± 0.0
Asp
2.355AspAla: 2.355 ± 0.326
0.442AspCys: 0.442 ± 0.117
4.092AspAsp: 4.092 ± 0.357
3.974AspGlu: 3.974 ± 0.326
3.179AspPhe: 3.179 ± 0.285
3.798AspGly: 3.798 ± 0.416
0.412AspHis: 0.412 ± 0.115
7.007AspIle: 7.007 ± 0.51
7.183AspLys: 7.183 ± 0.52
6.212AspLeu: 6.212 ± 0.523
1.737AspMet: 1.737 ± 0.189
5.387AspAsn: 5.387 ± 0.319
1.325AspPro: 1.325 ± 0.206
0.795AspGln: 0.795 ± 0.183
2.532AspArg: 2.532 ± 0.271
3.592AspSer: 3.592 ± 0.363
4.239AspThr: 4.239 ± 0.325
4.622AspVal: 4.622 ± 0.381
0.707AspTrp: 0.707 ± 0.153
3.974AspTyr: 3.974 ± 0.347
0.0AspXaa: 0.0 ± 0.0
Glu
3.562GluAla: 3.562 ± 0.366
0.442GluCys: 0.442 ± 0.13
5.564GluAsp: 5.564 ± 0.454
6.683GluGlu: 6.683 ± 0.888
2.532GluPhe: 2.532 ± 0.275
3.503GluGly: 3.503 ± 0.291
1.531GluHis: 1.531 ± 0.218
4.063GluIle: 4.063 ± 0.35
6.948GluLys: 6.948 ± 0.553
7.919GluLeu: 7.919 ± 0.584
1.855GluMet: 1.855 ± 0.2
4.239GluAsn: 4.239 ± 0.411
1.796GluPro: 1.796 ± 0.331
3.974GluGln: 3.974 ± 0.501
2.473GluArg: 2.473 ± 0.279
4.269GluSer: 4.269 ± 0.324
3.444GluThr: 3.444 ± 0.304
6.035GluVal: 6.035 ± 0.396
0.589GluTrp: 0.589 ± 0.125
4.151GluTyr: 4.151 ± 0.392
0.0GluXaa: 0.0 ± 0.0
Phe
1.295PheAla: 1.295 ± 0.183
0.265PheCys: 0.265 ± 0.094
2.237PheAsp: 2.237 ± 0.209
2.885PheGlu: 2.885 ± 0.283
1.178PhePhe: 1.178 ± 0.183
2.267PheGly: 2.267 ± 0.241
0.471PheHis: 0.471 ± 0.114
3.827PheIle: 3.827 ± 0.373
4.151PheLys: 4.151 ± 0.293
2.502PheLeu: 2.502 ± 0.247
0.854PheMet: 0.854 ± 0.154
3.121PheAsn: 3.121 ± 0.286
1.06PhePro: 1.06 ± 0.161
0.765PheGln: 0.765 ± 0.143
1.089PheArg: 1.089 ± 0.198
2.797PheSer: 2.797 ± 0.279
2.12PheThr: 2.12 ± 0.285
1.972PheVal: 1.972 ± 0.278
0.236PheTrp: 0.236 ± 0.092
2.208PheTyr: 2.208 ± 0.286
0.0PheXaa: 0.0 ± 0.0
Gly
2.237GlyAla: 2.237 ± 0.413
0.471GlyCys: 0.471 ± 0.135
3.503GlyAsp: 3.503 ± 0.407
2.944GlyGlu: 2.944 ± 0.285
2.002GlyPhe: 2.002 ± 0.234
3.503GlyGly: 3.503 ± 0.649
0.883GlyHis: 0.883 ± 0.168
4.122GlyIle: 4.122 ± 0.326
5.093GlyLys: 5.093 ± 0.486
3.768GlyLeu: 3.768 ± 0.349
1.089GlyMet: 1.089 ± 0.199
3.621GlyAsn: 3.621 ± 0.338
0.0GlyPro: 0.0 ± 0.0
1.678GlyGln: 1.678 ± 0.207
2.031GlyArg: 2.031 ± 0.236
3.533GlySer: 3.533 ± 0.395
3.709GlyThr: 3.709 ± 0.399
3.15GlyVal: 3.15 ± 0.349
0.648GlyTrp: 0.648 ± 0.153
3.503GlyTyr: 3.503 ± 0.333
0.0GlyXaa: 0.0 ± 0.0
His
0.648HisAla: 0.648 ± 0.123
0.177HisCys: 0.177 ± 0.095
0.883HisAsp: 0.883 ± 0.143
0.854HisGlu: 0.854 ± 0.152
0.648HisPhe: 0.648 ± 0.134
0.942HisGly: 0.942 ± 0.188
0.147HisHis: 0.147 ± 0.075
1.59HisIle: 1.59 ± 0.253
1.619HisLys: 1.619 ± 0.264
1.443HisLeu: 1.443 ± 0.25
0.559HisMet: 0.559 ± 0.131
0.942HisAsn: 0.942 ± 0.182
0.383HisPro: 0.383 ± 0.101
0.589HisGln: 0.589 ± 0.14
0.618HisArg: 0.618 ± 0.148
0.854HisSer: 0.854 ± 0.134
0.942HisThr: 0.942 ± 0.167
1.295HisVal: 1.295 ± 0.183
0.029HisTrp: 0.029 ± 0.028
0.854HisTyr: 0.854 ± 0.151
0.0HisXaa: 0.0 ± 0.0
Ile
2.738IleAla: 2.738 ± 0.283
0.559IleCys: 0.559 ± 0.138
6.094IleAsp: 6.094 ± 0.411
6.447IleGlu: 6.447 ± 0.532
2.179IlePhe: 2.179 ± 0.261
4.063IleGly: 4.063 ± 0.311
1.089IleHis: 1.089 ± 0.175
5.505IleIle: 5.505 ± 0.483
7.507IleLys: 7.507 ± 0.498
5.652IleLeu: 5.652 ± 0.448
1.443IleMet: 1.443 ± 0.227
5.8IleAsn: 5.8 ± 0.445
2.061IlePro: 2.061 ± 0.28
2.767IleGln: 2.767 ± 0.286
2.473IleArg: 2.473 ± 0.284
4.74IleSer: 4.74 ± 0.375
5.917IleThr: 5.917 ± 0.449
4.593IleVal: 4.593 ± 0.379
0.442IleTrp: 0.442 ± 0.117
3.032IleTyr: 3.032 ± 0.346
0.0IleXaa: 0.0 ± 0.0
Lys
4.033LysAla: 4.033 ± 0.397
0.765LysCys: 0.765 ± 0.193
8.449LysAsp: 8.449 ± 0.47
10.098LysGlu: 10.098 ± 0.797
3.003LysPhe: 3.003 ± 0.261
6.388LysGly: 6.388 ± 0.569
2.179LysHis: 2.179 ± 0.281
4.622LysIle: 4.622 ± 0.341
8.449LysLys: 8.449 ± 0.645
7.654LysLeu: 7.654 ± 0.612
1.649LysMet: 1.649 ± 0.23
6.536LysAsn: 6.536 ± 0.468
2.885LysPro: 2.885 ± 0.375
3.886LysGln: 3.886 ± 0.425
4.239LysArg: 4.239 ± 0.392
5.593LysSer: 5.593 ± 0.458
5.505LysThr: 5.505 ± 0.393
6.506LysVal: 6.506 ± 0.438
0.707LysTrp: 0.707 ± 0.143
5.888LysTyr: 5.888 ± 0.444
0.0LysXaa: 0.0 ± 0.0
Leu
3.945LeuAla: 3.945 ± 0.375
0.707LeuCys: 0.707 ± 0.139
6.153LeuAsp: 6.153 ± 0.492
6.329LeuGlu: 6.329 ± 0.43
3.209LeuPhe: 3.209 ± 0.33
3.709LeuGly: 3.709 ± 0.436
1.325LeuHis: 1.325 ± 0.215
5.329LeuIle: 5.329 ± 0.491
8.272LeuLys: 8.272 ± 0.512
6.506LeuLeu: 6.506 ± 0.491
2.061LeuMet: 2.061 ± 0.253
5.505LeuAsn: 5.505 ± 0.447
2.767LeuPro: 2.767 ± 0.261
3.444LeuGln: 3.444 ± 0.308
3.444LeuArg: 3.444 ± 0.383
6.212LeuSer: 6.212 ± 0.452
5.976LeuThr: 5.976 ± 0.448
4.504LeuVal: 4.504 ± 0.333
0.648LeuTrp: 0.648 ± 0.135
3.65LeuTyr: 3.65 ± 0.361
0.0LeuXaa: 0.0 ± 0.0
Met
1.207MetAla: 1.207 ± 0.203
0.147MetCys: 0.147 ± 0.059
1.207MetAsp: 1.207 ± 0.21
1.678MetGlu: 1.678 ± 0.237
0.913MetPhe: 0.913 ± 0.186
1.001MetGly: 1.001 ± 0.191
0.206MetHis: 0.206 ± 0.078
1.678MetIle: 1.678 ± 0.239
2.267MetLys: 2.267 ± 0.262
1.56MetLeu: 1.56 ± 0.198
0.648MetMet: 0.648 ± 0.133
1.178MetAsn: 1.178 ± 0.187
0.589MetPro: 0.589 ± 0.147
0.795MetGln: 0.795 ± 0.143
0.854MetArg: 0.854 ± 0.132
1.972MetSer: 1.972 ± 0.227
1.178MetThr: 1.178 ± 0.198
1.413MetVal: 1.413 ± 0.188
0.236MetTrp: 0.236 ± 0.091
1.207MetTyr: 1.207 ± 0.174
0.0MetXaa: 0.0 ± 0.0
Asn
2.591AsnAla: 2.591 ± 0.291
0.412AsnCys: 0.412 ± 0.107
3.768AsnAsp: 3.768 ± 0.305
4.622AsnGlu: 4.622 ± 0.457
2.267AsnPhe: 2.267 ± 0.269
3.238AsnGly: 3.238 ± 0.258
1.178AsnHis: 1.178 ± 0.175
5.917AsnIle: 5.917 ± 0.517
8.42AsnLys: 8.42 ± 0.545
6.329AsnLeu: 6.329 ± 0.509
1.295AsnMet: 1.295 ± 0.2
6.859AsnAsn: 6.859 ± 0.551
2.355AsnPro: 2.355 ± 0.265
2.561AsnGln: 2.561 ± 0.283
2.708AsnArg: 2.708 ± 0.297
3.474AsnSer: 3.474 ± 0.288
5.535AsnThr: 5.535 ± 0.395
4.21AsnVal: 4.21 ± 0.349
0.618AsnTrp: 0.618 ± 0.158
3.356AsnTyr: 3.356 ± 0.368
0.0AsnXaa: 0.0 ± 0.0
Pro
0.972ProAla: 0.972 ± 0.151
0.177ProCys: 0.177 ± 0.065
1.236ProAsp: 1.236 ± 0.18
2.061ProGlu: 2.061 ± 0.29
1.06ProPhe: 1.06 ± 0.179
0.559ProGly: 0.559 ± 0.177
0.383ProHis: 0.383 ± 0.094
2.267ProIle: 2.267 ± 0.364
2.738ProLys: 2.738 ± 0.249
2.237ProLeu: 2.237 ± 0.233
0.589ProMet: 0.589 ± 0.117
2.237ProAsn: 2.237 ± 0.271
0.589ProPro: 0.589 ± 0.139
1.089ProGln: 1.089 ± 0.25
0.972ProArg: 0.972 ± 0.188
1.59ProSer: 1.59 ± 0.185
2.031ProThr: 2.031 ± 0.262
1.354ProVal: 1.354 ± 0.212
0.059ProTrp: 0.059 ± 0.042
1.737ProTyr: 1.737 ± 0.283
0.0ProXaa: 0.0 ± 0.0
Gln
1.884GlnAla: 1.884 ± 0.271
0.294GlnCys: 0.294 ± 0.095
2.679GlnAsp: 2.679 ± 0.304
3.444GlnGlu: 3.444 ± 0.397
1.178GlnPhe: 1.178 ± 0.195
2.443GlnGly: 2.443 ± 0.274
0.442GlnHis: 0.442 ± 0.112
2.12GlnIle: 2.12 ± 0.246
2.473GlnLys: 2.473 ± 0.299
3.062GlnLeu: 3.062 ± 0.301
0.854GlnMet: 0.854 ± 0.168
1.531GlnAsn: 1.531 ± 0.233
0.795GlnPro: 0.795 ± 0.163
1.855GlnGln: 1.855 ± 0.334
1.443GlnArg: 1.443 ± 0.189
2.326GlnSer: 2.326 ± 0.279
1.649GlnThr: 1.649 ± 0.225
2.591GlnVal: 2.591 ± 0.271
0.294GlnTrp: 0.294 ± 0.089
1.56GlnTyr: 1.56 ± 0.22
0.0GlnXaa: 0.0 ± 0.0
Arg
1.59ArgAla: 1.59 ± 0.2
0.353ArgCys: 0.353 ± 0.126
2.561ArgAsp: 2.561 ± 0.276
2.443ArgGlu: 2.443 ± 0.267
2.002ArgPhe: 2.002 ± 0.24
1.914ArgGly: 1.914 ± 0.248
0.648ArgHis: 0.648 ± 0.15
2.296ArgIle: 2.296 ± 0.217
3.68ArgLys: 3.68 ± 0.365
3.268ArgLeu: 3.268 ± 0.325
1.03ArgMet: 1.03 ± 0.156
2.414ArgAsn: 2.414 ± 0.247
0.913ArgPro: 0.913 ± 0.165
1.266ArgGln: 1.266 ± 0.169
1.766ArgArg: 1.766 ± 0.283
1.737ArgSer: 1.737 ± 0.235
2.591ArgThr: 2.591 ± 0.283
2.65ArgVal: 2.65 ± 0.298
0.265ArgTrp: 0.265 ± 0.096
1.443ArgTyr: 1.443 ± 0.204
0.0ArgXaa: 0.0 ± 0.0
Ser
2.414SerAla: 2.414 ± 0.318
0.265SerCys: 0.265 ± 0.086
4.386SerAsp: 4.386 ± 0.371
4.18SerGlu: 4.18 ± 0.334
2.65SerPhe: 2.65 ± 0.257
2.856SerGly: 2.856 ± 0.394
0.972SerHis: 0.972 ± 0.182
5.535SerIle: 5.535 ± 0.502
6.889SerLys: 6.889 ± 0.456
5.358SerLeu: 5.358 ± 0.417
1.148SerMet: 1.148 ± 0.189
5.623SerAsn: 5.623 ± 0.431
1.266SerPro: 1.266 ± 0.192
1.737SerGln: 1.737 ± 0.2
1.825SerArg: 1.825 ± 0.276
4.769SerSer: 4.769 ± 0.574
4.504SerThr: 4.504 ± 0.428
3.65SerVal: 3.65 ± 0.345
0.648SerTrp: 0.648 ± 0.132
3.827SerTyr: 3.827 ± 0.354
0.0SerXaa: 0.0 ± 0.0
Thr
2.856ThrAla: 2.856 ± 0.36
0.294ThrCys: 0.294 ± 0.113
3.945ThrAsp: 3.945 ± 0.371
4.622ThrGlu: 4.622 ± 0.414
3.003ThrPhe: 3.003 ± 0.278
3.121ThrGly: 3.121 ± 0.336
1.295ThrHis: 1.295 ± 0.174
5.505ThrIle: 5.505 ± 0.393
6.035ThrLys: 6.035 ± 0.391
5.917ThrLeu: 5.917 ± 0.453
1.178ThrMet: 1.178 ± 0.176
4.416ThrAsn: 4.416 ± 0.374
2.65ThrPro: 2.65 ± 0.301
2.296ThrGln: 2.296 ± 0.268
2.208ThrArg: 2.208 ± 0.297
3.974ThrSer: 3.974 ± 0.335
4.004ThrThr: 4.004 ± 0.442
4.269ThrVal: 4.269 ± 0.372
0.589ThrTrp: 0.589 ± 0.143
3.238ThrTyr: 3.238 ± 0.349
0.0ThrXaa: 0.0 ± 0.0
Val
2.797ValAla: 2.797 ± 0.356
0.589ValCys: 0.589 ± 0.126
4.475ValAsp: 4.475 ± 0.328
5.27ValGlu: 5.27 ± 0.367
2.237ValPhe: 2.237 ± 0.261
2.826ValGly: 2.826 ± 0.294
1.001ValHis: 1.001 ± 0.175
4.534ValIle: 4.534 ± 0.358
5.741ValLys: 5.741 ± 0.374
5.122ValLeu: 5.122 ± 0.451
1.266ValMet: 1.266 ± 0.206
4.298ValAsn: 4.298 ± 0.393
1.737ValPro: 1.737 ± 0.237
1.943ValGln: 1.943 ± 0.246
2.267ValArg: 2.267 ± 0.292
5.505ValSer: 5.505 ± 0.433
4.357ValThr: 4.357 ± 0.385
4.18ValVal: 4.18 ± 0.438
0.412ValTrp: 0.412 ± 0.112
3.209ValTyr: 3.209 ± 0.324
0.0ValXaa: 0.0 ± 0.0
Trp
0.442TrpAla: 0.442 ± 0.126
0.059TrpCys: 0.059 ± 0.047
0.53TrpAsp: 0.53 ± 0.144
0.795TrpGlu: 0.795 ± 0.148
0.383TrpPhe: 0.383 ± 0.104
0.618TrpGly: 0.618 ± 0.145
0.029TrpHis: 0.029 ± 0.031
0.53TrpIle: 0.53 ± 0.135
0.913TrpLys: 0.913 ± 0.163
0.765TrpLeu: 0.765 ± 0.175
0.118TrpMet: 0.118 ± 0.073
0.559TrpAsn: 0.559 ± 0.113
0.0TrpPro: 0.0 ± 0.0
0.177TrpGln: 0.177 ± 0.072
0.147TrpArg: 0.147 ± 0.069
0.53TrpSer: 0.53 ± 0.117
0.442TrpThr: 0.442 ± 0.132
0.618TrpVal: 0.618 ± 0.135
0.265TrpTrp: 0.265 ± 0.102
0.589TrpTyr: 0.589 ± 0.16
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.002TyrAla: 2.002 ± 0.206
0.53TyrCys: 0.53 ± 0.14
3.327TyrAsp: 3.327 ± 0.314
2.973TyrGlu: 2.973 ± 0.346
1.825TyrPhe: 1.825 ± 0.255
2.385TyrGly: 2.385 ± 0.321
0.648TyrHis: 0.648 ± 0.127
4.593TyrIle: 4.593 ± 0.41
5.27TyrLys: 5.27 ± 0.34
4.504TyrLeu: 4.504 ± 0.448
1.266TyrMet: 1.266 ± 0.193
4.504TyrAsn: 4.504 ± 0.351
1.236TyrPro: 1.236 ± 0.185
1.59TyrGln: 1.59 ± 0.199
2.061TyrArg: 2.061 ± 0.247
3.15TyrSer: 3.15 ± 0.293
4.298TyrThr: 4.298 ± 0.424
3.415TyrVal: 3.415 ± 0.352
0.618TyrTrp: 0.618 ± 0.16
3.121TyrTyr: 3.121 ± 0.326
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 164 proteins (33969 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski