Circoviridae 14 LDMD-2013

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; environmental samples

Average proteome isoelectric point is 7.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5TMX4|S5TMX4_9CIRC ATP-dependent helicase Rep OS=Circoviridae 14 LDMD-2013 OX=1379718 PE=3 SV=1
MM1 pKa = 7.19YY2 pKa = 10.55LVIWLEE8 pKa = 3.73WAAKK12 pKa = 9.51HH13 pKa = 5.22KK14 pKa = 10.62QKK16 pKa = 10.56EE17 pKa = 4.09LSQLTSKK24 pKa = 10.25QMNYY28 pKa = 9.83LVPGAWISEE37 pKa = 4.17LSRR40 pKa = 11.84EE41 pKa = 4.59VAWTMSSEE49 pKa = 4.63RR50 pKa = 11.84IPDD53 pKa = 3.57VFVDD57 pKa = 4.59PGPDD61 pKa = 3.67DD62 pKa = 5.64DD63 pKa = 5.33LASNTVDD70 pKa = 3.21QSNKK74 pKa = 7.38RR75 pKa = 11.84TFWDD79 pKa = 3.38WVRR82 pKa = 11.84SMDD85 pKa = 5.13SDD87 pKa = 4.58DD88 pKa = 4.46FDD90 pKa = 4.31EE91 pKa = 6.6GDD93 pKa = 3.71VKK95 pKa = 10.87KK96 pKa = 10.7LKK98 pKa = 10.42QVTEE102 pKa = 4.38SMCMPTEE109 pKa = 3.67EE110 pKa = 4.56VNRR113 pKa = 11.84HH114 pKa = 4.39NHH116 pKa = 5.22PP117 pKa = 3.62

Molecular weight: 13.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5TNE8|S5TNE8_9CIRC Uncharacterized protein OS=Circoviridae 14 LDMD-2013 OX=1379718 PE=4 SV=1
MM1 pKa = 7.45PRR3 pKa = 11.84YY4 pKa = 9.32GKK6 pKa = 9.84RR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84YY10 pKa = 9.03RR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 9.89RR14 pKa = 11.84YY15 pKa = 8.13NKK17 pKa = 9.88KK18 pKa = 9.63YY19 pKa = 8.77MSRR22 pKa = 11.84FLRR25 pKa = 11.84AMLRR29 pKa = 11.84LGRR32 pKa = 11.84VRR34 pKa = 11.84TSKK37 pKa = 10.68GQSFGILTAGAGKK50 pKa = 9.91QDD52 pKa = 4.93AITVPLSARR61 pKa = 11.84IDD63 pKa = 3.83EE64 pKa = 4.91IYY66 pKa = 11.21NLLNRR71 pKa = 11.84KK72 pKa = 8.97GKK74 pKa = 10.28EE75 pKa = 3.8GEE77 pKa = 3.64IWPKK81 pKa = 10.89GFAFTIGAATVSSATHH97 pKa = 5.85ANFYY101 pKa = 10.86HH102 pKa = 7.06LGTIRR107 pKa = 11.84KK108 pKa = 7.63YY109 pKa = 8.06TFKK112 pKa = 11.22NQMNCPIEE120 pKa = 4.54AIFYY124 pKa = 9.07KK125 pKa = 10.48VQYY128 pKa = 9.83KK129 pKa = 10.44KK130 pKa = 10.7SFCSGEE136 pKa = 4.1LGSAASQAAFEE147 pKa = 4.39NFLMNAPVVLWNEE160 pKa = 4.36YY161 pKa = 9.71IGHH164 pKa = 5.3QVSTGTTASEE174 pKa = 4.23FVGVQSNAPSTVDD187 pKa = 3.27LTGDD191 pKa = 3.36YY192 pKa = 10.0DD193 pKa = 4.28AKK195 pKa = 11.4DD196 pKa = 3.9MEE198 pKa = 5.0TDD200 pKa = 3.4PQDD203 pKa = 3.59VQGEE207 pKa = 4.32DD208 pKa = 3.56VEE210 pKa = 6.33DD211 pKa = 4.36DD212 pKa = 3.93ANEE215 pKa = 4.24GGLPSGYY222 pKa = 8.76EE223 pKa = 3.41QHH225 pKa = 6.96KK226 pKa = 10.48YY227 pKa = 9.8YY228 pKa = 11.03GSGPGSSKK236 pKa = 10.65YY237 pKa = 9.48FRR239 pKa = 11.84EE240 pKa = 4.4YY241 pKa = 10.55INVIKK246 pKa = 9.78VQKK249 pKa = 10.64VVMRR253 pKa = 11.84TNQLVNISHH262 pKa = 7.4ISKK265 pKa = 10.6KK266 pKa = 7.39MRR268 pKa = 11.84KK269 pKa = 9.08YY270 pKa = 10.45DD271 pKa = 3.71AEE273 pKa = 4.05EE274 pKa = 3.86LKK276 pKa = 11.11AFANAYY282 pKa = 9.69QGASSNVGAGARR294 pKa = 11.84IHH296 pKa = 6.91RR297 pKa = 11.84KK298 pKa = 9.39GDD300 pKa = 3.11VDD302 pKa = 3.71VMVVVRR308 pKa = 11.84GLPGVRR314 pKa = 11.84TITAADD320 pKa = 3.56VTEE323 pKa = 5.17DD324 pKa = 3.15KK325 pKa = 11.34AQIGWEE331 pKa = 4.25SGVHH335 pKa = 5.05EE336 pKa = 4.56PAVATLNSNIVTTSPASLVWTHH358 pKa = 6.4QIYY361 pKa = 10.67HH362 pKa = 6.19KK363 pKa = 10.22VGWMPTGGYY372 pKa = 7.26PQHH375 pKa = 7.09SYY377 pKa = 11.18GSRR380 pKa = 11.84FYY382 pKa = 11.03YY383 pKa = 8.52PTGTYY388 pKa = 9.95EE389 pKa = 3.79VDD391 pKa = 3.14ADD393 pKa = 3.93GADD396 pKa = 3.29AHH398 pKa = 7.01LSLSTFNATKK408 pKa = 10.45VDD410 pKa = 3.73SS411 pKa = 3.97

Molecular weight: 45.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

808

117

411

269.3

30.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.054 ± 1.382

0.619 ± 0.1

5.446 ± 1.352

5.941 ± 0.789

3.837 ± 0.407

7.302 ± 1.679

2.599 ± 0.051

5.074 ± 0.83

7.054 ± 0.341

6.683 ± 1.203

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.351 ± 0.805

5.322 ± 0.601

4.332 ± 0.467

3.96 ± 0.092

5.322 ± 0.319

7.054 ± 0.801

6.188 ± 0.355

6.807 ± 0.895

1.98 ± 0.942

5.074 ± 0.995
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski