Moraxella phage Mcat15

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 42 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R6PI81|A0A0R6PI81_9CAUD Plate protein OS=Moraxella phage Mcat15 OX=1647530 PE=4 SV=1
MM1 pKa = 7.39IASKK5 pKa = 10.57RR6 pKa = 11.84IFGLLGEE13 pKa = 4.56LVNDD17 pKa = 3.65RR18 pKa = 11.84VYY20 pKa = 11.12PLFVPEE26 pKa = 4.74TADD29 pKa = 3.26STPPFMVYY37 pKa = 9.4TLVSNVPDD45 pKa = 3.65NTLDD49 pKa = 3.51GATGHH54 pKa = 6.12EE55 pKa = 4.34WVGVQIDD62 pKa = 4.68IYY64 pKa = 11.07AADD67 pKa = 4.0YY68 pKa = 11.55DD69 pKa = 4.13EE70 pKa = 5.35TIALAQEE77 pKa = 3.92AVKK80 pKa = 10.44RR81 pKa = 11.84LNTIKK86 pKa = 10.09PSEE89 pKa = 4.32YY90 pKa = 9.31GGVVYY95 pKa = 10.9VHH97 pKa = 7.72DD98 pKa = 5.59DD99 pKa = 3.19GLYY102 pKa = 9.37RR103 pKa = 11.84AIIEE107 pKa = 4.2YY108 pKa = 9.94EE109 pKa = 4.13FWQTIAA115 pKa = 4.44

Molecular weight: 12.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R6PIQ9|A0A0R6PIQ9_9CAUD Uncharacterized protein OS=Moraxella phage Mcat15 OX=1647530 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.65LIGAEE7 pKa = 3.97EE8 pKa = 4.17LGKK11 pKa = 10.51KK12 pKa = 9.91LKK14 pKa = 9.25QARR17 pKa = 11.84EE18 pKa = 4.11EE19 pKa = 4.13ITNKK23 pKa = 9.69QAGSALYY30 pKa = 10.23SSLMYY35 pKa = 10.73ASLPMYY41 pKa = 10.27KK42 pKa = 9.79YY43 pKa = 10.7VRR45 pKa = 11.84SQAPMTKK52 pKa = 9.95APYY55 pKa = 9.03RR56 pKa = 11.84RR57 pKa = 11.84YY58 pKa = 9.39MSYY61 pKa = 10.7GQGDD65 pKa = 3.89GFYY68 pKa = 10.58YY69 pKa = 10.71DD70 pKa = 3.84KK71 pKa = 11.18NGKK74 pKa = 8.5KK75 pKa = 9.5RR76 pKa = 11.84RR77 pKa = 11.84RR78 pKa = 11.84RR79 pKa = 11.84AKK81 pKa = 10.17RR82 pKa = 11.84GTGKK86 pKa = 10.76YY87 pKa = 9.06EE88 pKa = 3.75IQEE91 pKa = 3.88SGLYY95 pKa = 9.66HH96 pKa = 6.87KK97 pKa = 10.33SIKK100 pKa = 9.76RR101 pKa = 11.84RR102 pKa = 11.84RR103 pKa = 11.84LTRR106 pKa = 11.84GKK108 pKa = 9.99SANLDD113 pKa = 3.32GAAIAIYY120 pKa = 10.0VAEE123 pKa = 4.83GSGKK127 pKa = 6.88THH129 pKa = 6.47GSAYY133 pKa = 9.41YY134 pKa = 9.84WFFNEE139 pKa = 5.1YY140 pKa = 7.37GTKK143 pKa = 9.57YY144 pKa = 9.61QAARR148 pKa = 11.84PIFRR152 pKa = 11.84PAFNSGAEE160 pKa = 4.06DD161 pKa = 3.33AADD164 pKa = 3.61RR165 pKa = 11.84FKK167 pKa = 11.69NKK169 pKa = 10.02LGEE172 pKa = 4.61RR173 pKa = 11.84IDD175 pKa = 4.3KK176 pKa = 10.95IMGGG180 pKa = 3.57

Molecular weight: 20.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

42

0

42

9772

46

1631

232.7

25.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.975 ± 0.437

0.88 ± 0.185

5.843 ± 0.274

6.079 ± 0.397

3.541 ± 0.226

6.897 ± 0.368

1.995 ± 0.221

6.672 ± 0.346

7.255 ± 0.47

8.576 ± 0.305

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.446 ± 0.31

5.209 ± 0.381

3.06 ± 0.243

4.513 ± 0.177

4.728 ± 0.282

6.826 ± 0.57

5.905 ± 0.518

6.13 ± 0.245

1.279 ± 0.13

3.193 ± 0.197
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski