Nothophytophthora sp. Chile5

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae; Nothophytophthora; unclassified Nothophytophthora

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 26905 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A662WEA1|A0A662WEA1_9STRA RING-type domain-containing protein OS=Nothophytophthora sp. Chile5 OX=2483409 GN=BBJ28_00020215 PE=4 SV=1
MM1 pKa = 7.41LSAAAFVSAAALLAASVDD19 pKa = 3.15AHH21 pKa = 7.35GYY23 pKa = 7.98MLIPEE28 pKa = 4.29SQFTGSANSAWIVQIDD44 pKa = 4.04PQWSSDD50 pKa = 2.97AWDD53 pKa = 4.26GNNQGSVDD61 pKa = 3.55AFVAAKK67 pKa = 9.88SANGYY72 pKa = 8.0TDD74 pKa = 5.06LRR76 pKa = 11.84TLMDD80 pKa = 3.78DD81 pKa = 3.46TSVYY85 pKa = 10.74GADD88 pKa = 4.3CGFTNPDD95 pKa = 2.78GTAQPVPTDD104 pKa = 3.38SKK106 pKa = 10.66ATFSRR111 pKa = 11.84ALVHH115 pKa = 6.79VGPCEE120 pKa = 3.49IWLDD124 pKa = 3.78DD125 pKa = 3.96TKK127 pKa = 11.53VLAEE131 pKa = 4.5DD132 pKa = 4.14DD133 pKa = 4.46CYY135 pKa = 11.69SLYY138 pKa = 11.16GNEE141 pKa = 4.22DD142 pKa = 3.1QDD144 pKa = 3.45IKK146 pKa = 11.41SIFPVDD152 pKa = 3.68YY153 pKa = 10.5SSCDD157 pKa = 3.33TAGCSMMRR165 pKa = 11.84FYY167 pKa = 10.87WLAFQGVDD175 pKa = 4.97GDD177 pKa = 4.86TVWQSYY183 pKa = 9.94KK184 pKa = 11.04DD185 pKa = 4.16CIPLEE190 pKa = 4.64GGSGTSSSTTTTDD203 pKa = 2.97APASEE208 pKa = 5.14ASATDD213 pKa = 3.62APSTDD218 pKa = 3.96APSTDD223 pKa = 4.13APATDD228 pKa = 4.8APTTDD233 pKa = 4.45APTTDD238 pKa = 4.45APTTDD243 pKa = 3.45APAAAEE249 pKa = 4.27TPEE252 pKa = 4.18VTPAPSDD259 pKa = 3.08KK260 pKa = 10.3CTGRR264 pKa = 11.84KK265 pKa = 8.85RR266 pKa = 11.84RR267 pKa = 11.84NN268 pKa = 3.03

Molecular weight: 28.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A662X0T5|A0A662X0T5_9STRA Uncharacterized protein OS=Nothophytophthora sp. Chile5 OX=2483409 GN=BBJ28_00023662 PE=4 SV=1
MM1 pKa = 7.91PSNGCRR7 pKa = 11.84ISCILATIEE16 pKa = 5.31DD17 pKa = 3.9EE18 pKa = 4.52DD19 pKa = 3.9EE20 pKa = 4.16RR21 pKa = 11.84VATGVMTAFLSGVILLLVRR40 pKa = 11.84ILNLRR45 pKa = 11.84QVTDD49 pKa = 4.29FFSRR53 pKa = 11.84PVMDD57 pKa = 4.4GFISAGGLLIMTCSKK72 pKa = 10.7SRR74 pKa = 11.84WATRR78 pKa = 11.84HH79 pKa = 3.52TRR81 pKa = 11.84RR82 pKa = 11.84RR83 pKa = 11.84MFQFFKK89 pKa = 10.75HH90 pKa = 5.72VGEE93 pKa = 4.66SKK95 pKa = 10.31PAALGLFKK103 pKa = 10.63KK104 pKa = 10.46RR105 pKa = 11.84PQCATRR111 pKa = 11.84HH112 pKa = 5.33FPRR115 pKa = 11.84THH117 pKa = 6.06HH118 pKa = 6.1VLPGLLVVCIGGGIAGYY135 pKa = 10.64LLGPKK140 pKa = 9.53ALKK143 pKa = 10.22LAANVPGGFPPPDD156 pKa = 3.74APWYY160 pKa = 9.96GFTSGLIVGGTILLNASTVSLVVFLSSIAMTKK192 pKa = 10.15RR193 pKa = 11.84LAIQRR198 pKa = 11.84GEE200 pKa = 4.47DD201 pKa = 3.14INTKK205 pKa = 10.0QEE207 pKa = 4.01LTGVGCASIVCGFFRR222 pKa = 11.84TMRR225 pKa = 11.84PTGGVSRR232 pKa = 11.84TAVNLQNAHH241 pKa = 5.6IQVPRR246 pKa = 11.84RR247 pKa = 11.84SLFSPRR253 pKa = 11.84CTFRR257 pKa = 11.84GTLYY261 pKa = 10.05YY262 pKa = 10.53LPRR265 pKa = 11.84AALASIIIATSYY277 pKa = 11.07SLPPMM282 pKa = 4.55

Molecular weight: 30.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

26905

0

26905

13882063

49

10584

516.0

56.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.217 ± 0.018

1.607 ± 0.006

5.597 ± 0.01

6.691 ± 0.015

3.696 ± 0.009

6.376 ± 0.014

2.327 ± 0.006

3.544 ± 0.011

4.439 ± 0.015

9.808 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.306 ± 0.007

3.026 ± 0.007

4.853 ± 0.012

4.33 ± 0.01

6.427 ± 0.016

8.197 ± 0.015

5.751 ± 0.012

7.231 ± 0.011

1.195 ± 0.005

2.382 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski