Amino acid dipepetide frequency for Aeromonas phage PVN02

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.341AlaAla: 10.341 ± 1.113
1.745AlaCys: 1.745 ± 0.303
5.235AlaAsp: 5.235 ± 0.526
6.722AlaGlu: 6.722 ± 0.549
2.715AlaPhe: 2.715 ± 0.327
6.722AlaGly: 6.722 ± 0.606
2.198AlaHis: 2.198 ± 0.404
4.783AlaIle: 4.783 ± 0.664
4.847AlaLys: 4.847 ± 0.615
7.627AlaLeu: 7.627 ± 0.897
2.456AlaMet: 2.456 ± 0.371
3.813AlaAsn: 3.813 ± 0.434
3.878AlaPro: 3.878 ± 0.517
3.813AlaGln: 3.813 ± 0.423
5.558AlaArg: 5.558 ± 0.564
3.49AlaSer: 3.49 ± 0.597
4.912AlaThr: 4.912 ± 0.605
8.208AlaVal: 8.208 ± 0.702
0.905AlaTrp: 0.905 ± 0.213
2.198AlaTyr: 2.198 ± 0.4
0.0AlaXaa: 0.0 ± 0.0
Cys
0.388CysAla: 0.388 ± 0.168
0.194CysCys: 0.194 ± 0.13
0.517CysAsp: 0.517 ± 0.15
0.776CysGlu: 0.776 ± 0.344
0.388CysPhe: 0.388 ± 0.195
1.163CysGly: 1.163 ± 0.265
0.323CysHis: 0.323 ± 0.164
1.163CysIle: 1.163 ± 0.433
0.905CysLys: 0.905 ± 0.277
1.099CysLeu: 1.099 ± 0.276
0.323CysMet: 0.323 ± 0.138
0.646CysAsn: 0.646 ± 0.192
0.711CysPro: 0.711 ± 0.202
0.582CysGln: 0.582 ± 0.169
1.034CysArg: 1.034 ± 0.227
0.711CysSer: 0.711 ± 0.218
0.776CysThr: 0.776 ± 0.197
0.711CysVal: 0.711 ± 0.271
0.065CysTrp: 0.065 ± 0.067
0.452CysTyr: 0.452 ± 0.191
0.0CysXaa: 0.0 ± 0.0
Asp
6.011AspAla: 6.011 ± 0.673
0.517AspCys: 0.517 ± 0.2
3.232AspAsp: 3.232 ± 0.558
3.619AspGlu: 3.619 ± 0.462
1.81AspPhe: 1.81 ± 0.316
5.429AspGly: 5.429 ± 0.397
1.293AspHis: 1.293 ± 0.282
3.555AspIle: 3.555 ± 0.589
3.619AspLys: 3.619 ± 0.417
5.171AspLeu: 5.171 ± 0.558
1.68AspMet: 1.68 ± 0.294
2.65AspAsn: 2.65 ± 0.395
3.555AspPro: 3.555 ± 0.636
2.262AspGln: 2.262 ± 0.355
2.585AspArg: 2.585 ± 0.447
2.908AspSer: 2.908 ± 0.379
3.102AspThr: 3.102 ± 0.372
4.589AspVal: 4.589 ± 0.532
1.099AspTrp: 1.099 ± 0.226
1.357AspTyr: 1.357 ± 0.302
0.0AspXaa: 0.0 ± 0.0
Glu
6.334GluAla: 6.334 ± 0.861
0.776GluCys: 0.776 ± 0.257
3.49GluAsp: 3.49 ± 0.527
4.977GluGlu: 4.977 ± 0.702
1.81GluPhe: 1.81 ± 0.323
4.266GluGly: 4.266 ± 0.514
1.551GluHis: 1.551 ± 0.419
4.783GluIle: 4.783 ± 0.57
3.813GluLys: 3.813 ± 0.487
5.494GluLeu: 5.494 ± 0.621
2.65GluMet: 2.65 ± 0.419
2.715GluAsn: 2.715 ± 0.387
2.715GluPro: 2.715 ± 0.398
2.844GluGln: 2.844 ± 0.466
3.555GluArg: 3.555 ± 0.617
3.555GluSer: 3.555 ± 0.391
3.426GluThr: 3.426 ± 0.473
5.171GluVal: 5.171 ± 0.541
1.034GluTrp: 1.034 ± 0.281
3.038GluTyr: 3.038 ± 0.44
0.0GluXaa: 0.0 ± 0.0
Phe
2.585PheAla: 2.585 ± 0.433
0.776PheCys: 0.776 ± 0.214
2.585PheAsp: 2.585 ± 0.375
3.813PheGlu: 3.813 ± 0.586
1.228PhePhe: 1.228 ± 0.312
3.296PheGly: 3.296 ± 0.494
0.517PheHis: 0.517 ± 0.166
1.745PheIle: 1.745 ± 0.429
1.81PheLys: 1.81 ± 0.334
2.391PheLeu: 2.391 ± 0.339
1.228PheMet: 1.228 ± 0.336
1.939PheAsn: 1.939 ± 0.423
1.228PhePro: 1.228 ± 0.26
0.969PheGln: 0.969 ± 0.238
1.487PheArg: 1.487 ± 0.364
2.068PheSer: 2.068 ± 0.382
2.004PheThr: 2.004 ± 0.329
1.357PheVal: 1.357 ± 0.379
0.452PheTrp: 0.452 ± 0.166
0.711PheTyr: 0.711 ± 0.184
0.0PheXaa: 0.0 ± 0.0
Gly
5.817GlyAla: 5.817 ± 0.67
0.84GlyCys: 0.84 ± 0.259
5.041GlyAsp: 5.041 ± 0.628
4.46GlyGlu: 4.46 ± 0.62
3.102GlyPhe: 3.102 ± 0.483
5.365GlyGly: 5.365 ± 1.006
0.84GlyHis: 0.84 ± 0.28
4.654GlyIle: 4.654 ± 0.581
5.171GlyLys: 5.171 ± 0.666
5.752GlyLeu: 5.752 ± 0.583
2.262GlyMet: 2.262 ± 0.365
2.585GlyAsn: 2.585 ± 0.465
2.068GlyPro: 2.068 ± 0.428
2.65GlyGln: 2.65 ± 0.373
4.977GlyArg: 4.977 ± 0.64
3.038GlySer: 3.038 ± 0.402
4.395GlyThr: 4.395 ± 0.541
6.722GlyVal: 6.722 ± 0.971
1.422GlyTrp: 1.422 ± 0.324
3.102GlyTyr: 3.102 ± 0.51
0.0GlyXaa: 0.0 ± 0.0
His
1.293HisAla: 1.293 ± 0.262
0.388HisCys: 0.388 ± 0.146
1.487HisAsp: 1.487 ± 0.332
1.422HisGlu: 1.422 ± 0.228
0.905HisPhe: 0.905 ± 0.236
2.004HisGly: 2.004 ± 0.309
0.259HisHis: 0.259 ± 0.137
1.163HisIle: 1.163 ± 0.223
1.293HisLys: 1.293 ± 0.269
1.487HisLeu: 1.487 ± 0.3
0.452HisMet: 0.452 ± 0.16
1.422HisAsn: 1.422 ± 0.261
1.228HisPro: 1.228 ± 0.273
1.099HisGln: 1.099 ± 0.221
0.969HisArg: 0.969 ± 0.206
1.099HisSer: 1.099 ± 0.296
0.905HisThr: 0.905 ± 0.247
2.198HisVal: 2.198 ± 0.279
0.452HisTrp: 0.452 ± 0.15
0.517HisTyr: 0.517 ± 0.177
0.0HisXaa: 0.0 ± 0.0
Ile
6.334IleAla: 6.334 ± 0.729
0.711IleCys: 0.711 ± 0.214
4.395IleAsp: 4.395 ± 0.393
4.783IleGlu: 4.783 ± 0.558
1.357IlePhe: 1.357 ± 0.315
2.973IleGly: 2.973 ± 0.409
1.487IleHis: 1.487 ± 0.284
2.65IleIle: 2.65 ± 0.546
3.555IleLys: 3.555 ± 0.461
2.715IleLeu: 2.715 ± 0.503
1.228IleMet: 1.228 ± 0.305
2.844IleAsn: 2.844 ± 0.371
2.585IlePro: 2.585 ± 0.375
1.81IleGln: 1.81 ± 0.271
3.296IleArg: 3.296 ± 0.384
2.779IleSer: 2.779 ± 0.418
3.878IleThr: 3.878 ± 0.569
3.749IleVal: 3.749 ± 0.577
0.711IleTrp: 0.711 ± 0.234
0.84IleTyr: 0.84 ± 0.27
0.0IleXaa: 0.0 ± 0.0
Lys
6.269LysAla: 6.269 ± 0.592
0.646LysCys: 0.646 ± 0.204
3.878LysAsp: 3.878 ± 0.567
2.715LysGlu: 2.715 ± 0.519
2.65LysPhe: 2.65 ± 0.293
3.102LysGly: 3.102 ± 0.502
1.228LysHis: 1.228 ± 0.305
3.426LysIle: 3.426 ± 0.415
3.49LysLys: 3.49 ± 0.563
4.395LysLeu: 4.395 ± 0.626
1.939LysMet: 1.939 ± 0.397
1.874LysAsn: 1.874 ± 0.379
2.973LysPro: 2.973 ± 0.437
1.81LysGln: 1.81 ± 0.365
3.167LysArg: 3.167 ± 0.495
4.783LysSer: 4.783 ± 0.608
3.555LysThr: 3.555 ± 0.628
4.266LysVal: 4.266 ± 0.437
0.84LysTrp: 0.84 ± 0.283
2.004LysTyr: 2.004 ± 0.37
0.0LysXaa: 0.0 ± 0.0
Leu
6.528LeuAla: 6.528 ± 0.585
0.969LeuCys: 0.969 ± 0.248
4.718LeuAsp: 4.718 ± 0.546
6.657LeuGlu: 6.657 ± 0.781
1.81LeuPhe: 1.81 ± 0.367
5.3LeuGly: 5.3 ± 0.586
1.228LeuHis: 1.228 ± 0.252
3.813LeuIle: 3.813 ± 0.403
4.266LeuLys: 4.266 ± 0.617
6.269LeuLeu: 6.269 ± 0.777
2.004LeuMet: 2.004 ± 0.314
3.878LeuAsn: 3.878 ± 0.354
3.749LeuPro: 3.749 ± 0.431
4.007LeuGln: 4.007 ± 0.436
5.235LeuArg: 5.235 ± 0.572
5.752LeuSer: 5.752 ± 0.563
3.555LeuThr: 3.555 ± 0.493
4.912LeuVal: 4.912 ± 0.489
0.84LeuTrp: 0.84 ± 0.225
2.133LeuTyr: 2.133 ± 0.415
0.0LeuXaa: 0.0 ± 0.0
Met
2.456MetAla: 2.456 ± 0.409
0.452MetCys: 0.452 ± 0.175
1.68MetAsp: 1.68 ± 0.34
1.68MetGlu: 1.68 ± 0.34
0.905MetPhe: 0.905 ± 0.232
1.745MetGly: 1.745 ± 0.31
0.84MetHis: 0.84 ± 0.173
1.228MetIle: 1.228 ± 0.302
1.874MetLys: 1.874 ± 0.359
2.456MetLeu: 2.456 ± 0.438
0.84MetMet: 0.84 ± 0.185
1.034MetAsn: 1.034 ± 0.279
1.745MetPro: 1.745 ± 0.272
1.357MetGln: 1.357 ± 0.322
1.551MetArg: 1.551 ± 0.345
2.521MetSer: 2.521 ± 0.432
2.133MetThr: 2.133 ± 0.423
1.293MetVal: 1.293 ± 0.251
0.323MetTrp: 0.323 ± 0.146
1.357MetTyr: 1.357 ± 0.279
0.0MetXaa: 0.0 ± 0.0
Asn
4.266AsnAla: 4.266 ± 0.545
0.129AsnCys: 0.129 ± 0.081
2.068AsnAsp: 2.068 ± 0.372
1.745AsnGlu: 1.745 ± 0.39
1.422AsnPhe: 1.422 ± 0.304
4.524AsnGly: 4.524 ± 0.738
0.84AsnHis: 0.84 ± 0.2
1.939AsnIle: 1.939 ± 0.364
2.585AsnLys: 2.585 ± 0.32
2.973AsnLeu: 2.973 ± 0.466
1.034AsnMet: 1.034 ± 0.322
1.745AsnAsn: 1.745 ± 0.323
2.585AsnPro: 2.585 ± 0.47
1.68AsnGln: 1.68 ± 0.343
2.779AsnArg: 2.779 ± 0.372
2.456AsnSer: 2.456 ± 0.329
3.038AsnThr: 3.038 ± 0.398
2.715AsnVal: 2.715 ± 0.45
0.905AsnTrp: 0.905 ± 0.217
1.293AsnTyr: 1.293 ± 0.254
0.0AsnXaa: 0.0 ± 0.0
Pro
4.395ProAla: 4.395 ± 0.506
0.323ProCys: 0.323 ± 0.14
2.004ProAsp: 2.004 ± 0.332
3.813ProGlu: 3.813 ± 0.447
1.487ProPhe: 1.487 ± 0.37
3.813ProGly: 3.813 ± 0.595
1.163ProHis: 1.163 ± 0.32
1.616ProIle: 1.616 ± 0.408
2.844ProLys: 2.844 ± 0.476
3.619ProLeu: 3.619 ± 0.589
1.228ProMet: 1.228 ± 0.38
1.81ProAsn: 1.81 ± 0.317
1.099ProPro: 1.099 ± 0.365
1.939ProGln: 1.939 ± 0.342
1.68ProArg: 1.68 ± 0.339
2.65ProSer: 2.65 ± 0.45
3.038ProThr: 3.038 ± 0.543
3.943ProVal: 3.943 ± 0.42
0.452ProTrp: 0.452 ± 0.188
1.745ProTyr: 1.745 ± 0.438
0.0ProXaa: 0.0 ± 0.0
Gln
3.102GlnAla: 3.102 ± 0.37
0.323GlnCys: 0.323 ± 0.175
1.616GlnAsp: 1.616 ± 0.258
2.262GlnGlu: 2.262 ± 0.386
1.874GlnPhe: 1.874 ± 0.289
2.779GlnGly: 2.779 ± 0.341
1.228GlnHis: 1.228 ± 0.292
1.874GlnIle: 1.874 ± 0.351
2.004GlnLys: 2.004 ± 0.348
3.555GlnLeu: 3.555 ± 0.531
1.228GlnMet: 1.228 ± 0.304
1.616GlnAsn: 1.616 ± 0.307
1.422GlnPro: 1.422 ± 0.396
2.198GlnGln: 2.198 ± 0.452
2.908GlnArg: 2.908 ± 0.397
2.262GlnSer: 2.262 ± 0.471
1.874GlnThr: 1.874 ± 0.35
2.715GlnVal: 2.715 ± 0.465
0.711GlnTrp: 0.711 ± 0.225
1.616GlnTyr: 1.616 ± 0.26
0.0GlnXaa: 0.0 ± 0.0
Arg
5.429ArgAla: 5.429 ± 0.735
1.034ArgCys: 1.034 ± 0.31
3.619ArgAsp: 3.619 ± 0.363
4.072ArgGlu: 4.072 ± 0.488
2.004ArgPhe: 2.004 ± 0.368
3.555ArgGly: 3.555 ± 0.523
1.293ArgHis: 1.293 ± 0.248
3.361ArgIle: 3.361 ± 0.373
3.943ArgLys: 3.943 ± 0.562
4.718ArgLeu: 4.718 ± 0.508
1.81ArgMet: 1.81 ± 0.34
1.745ArgAsn: 1.745 ± 0.316
1.551ArgPro: 1.551 ± 0.442
1.551ArgGln: 1.551 ± 0.322
3.684ArgArg: 3.684 ± 0.499
3.619ArgSer: 3.619 ± 0.478
2.973ArgThr: 2.973 ± 0.436
4.137ArgVal: 4.137 ± 0.476
0.711ArgTrp: 0.711 ± 0.178
1.939ArgTyr: 1.939 ± 0.283
0.0ArgXaa: 0.0 ± 0.0
Ser
4.847SerAla: 4.847 ± 0.512
0.84SerCys: 0.84 ± 0.232
3.749SerAsp: 3.749 ± 0.498
3.296SerGlu: 3.296 ± 0.4
2.327SerPhe: 2.327 ± 0.474
4.718SerGly: 4.718 ± 0.519
1.551SerHis: 1.551 ± 0.482
2.908SerIle: 2.908 ± 0.414
2.65SerLys: 2.65 ± 0.389
4.654SerLeu: 4.654 ± 0.673
1.357SerMet: 1.357 ± 0.354
1.81SerAsn: 1.81 ± 0.314
2.585SerPro: 2.585 ± 0.345
2.327SerGln: 2.327 ± 0.419
2.779SerArg: 2.779 ± 0.451
2.844SerSer: 2.844 ± 0.461
3.167SerThr: 3.167 ± 0.405
4.395SerVal: 4.395 ± 0.469
0.646SerTrp: 0.646 ± 0.188
1.68SerTyr: 1.68 ± 0.286
0.0SerXaa: 0.0 ± 0.0
Thr
5.106ThrAla: 5.106 ± 0.6
0.517ThrCys: 0.517 ± 0.159
3.296ThrAsp: 3.296 ± 0.435
3.102ThrGlu: 3.102 ± 0.481
1.939ThrPhe: 1.939 ± 0.421
5.235ThrGly: 5.235 ± 0.515
0.84ThrHis: 0.84 ± 0.285
3.102ThrIle: 3.102 ± 0.454
3.361ThrLys: 3.361 ± 0.419
4.783ThrLeu: 4.783 ± 0.516
1.422ThrMet: 1.422 ± 0.352
2.521ThrAsn: 2.521 ± 0.472
4.007ThrPro: 4.007 ± 0.566
1.551ThrGln: 1.551 ± 0.286
2.779ThrArg: 2.779 ± 0.454
2.715ThrSer: 2.715 ± 0.437
3.232ThrThr: 3.232 ± 0.638
4.137ThrVal: 4.137 ± 0.541
1.357ThrTrp: 1.357 ± 0.249
2.262ThrTyr: 2.262 ± 0.516
0.0ThrXaa: 0.0 ± 0.0
Val
6.269ValAla: 6.269 ± 0.832
0.84ValCys: 0.84 ± 0.246
4.654ValAsp: 4.654 ± 0.509
4.783ValGlu: 4.783 ± 0.616
3.038ValPhe: 3.038 ± 0.437
4.46ValGly: 4.46 ± 0.502
1.939ValHis: 1.939 ± 0.436
4.072ValIle: 4.072 ± 0.666
4.718ValLys: 4.718 ± 0.489
4.524ValLeu: 4.524 ± 0.606
3.038ValMet: 3.038 ± 0.491
3.426ValAsn: 3.426 ± 0.475
3.102ValPro: 3.102 ± 0.379
2.65ValGln: 2.65 ± 0.458
4.266ValArg: 4.266 ± 0.533
3.296ValSer: 3.296 ± 0.475
4.201ValThr: 4.201 ± 0.669
5.365ValVal: 5.365 ± 0.561
1.293ValTrp: 1.293 ± 0.332
2.521ValTyr: 2.521 ± 0.472
0.0ValXaa: 0.0 ± 0.0
Trp
1.487TrpAla: 1.487 ± 0.317
0.194TrpCys: 0.194 ± 0.102
0.646TrpAsp: 0.646 ± 0.246
1.487TrpGlu: 1.487 ± 0.303
0.388TrpPhe: 0.388 ± 0.192
0.905TrpGly: 0.905 ± 0.253
0.582TrpHis: 0.582 ± 0.175
0.646TrpIle: 0.646 ± 0.193
0.776TrpLys: 0.776 ± 0.239
1.357TrpLeu: 1.357 ± 0.302
0.323TrpMet: 0.323 ± 0.144
1.099TrpAsn: 1.099 ± 0.323
0.323TrpPro: 0.323 ± 0.127
0.969TrpGln: 0.969 ± 0.222
0.776TrpArg: 0.776 ± 0.185
0.517TrpSer: 0.517 ± 0.144
0.646TrpThr: 0.646 ± 0.194
0.776TrpVal: 0.776 ± 0.226
0.129TrpTrp: 0.129 ± 0.072
0.84TrpTyr: 0.84 ± 0.205
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.908TyrAla: 2.908 ± 0.439
0.711TyrCys: 0.711 ± 0.236
2.133TyrAsp: 2.133 ± 0.369
1.81TyrGlu: 1.81 ± 0.29
1.034TyrPhe: 1.034 ± 0.346
2.908TyrGly: 2.908 ± 0.496
0.84TyrHis: 0.84 ± 0.216
2.198TyrIle: 2.198 ± 0.344
1.487TyrLys: 1.487 ± 0.332
2.585TyrLeu: 2.585 ± 0.459
0.905TyrMet: 0.905 ± 0.178
1.551TyrAsn: 1.551 ± 0.301
1.551TyrPro: 1.551 ± 0.318
1.099TyrGln: 1.099 ± 0.331
1.68TyrArg: 1.68 ± 0.357
2.133TyrSer: 2.133 ± 0.454
2.456TyrThr: 2.456 ± 0.387
1.099TyrVal: 1.099 ± 0.267
0.517TyrTrp: 0.517 ± 0.14
0.969TyrTyr: 0.969 ± 0.283
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 64 proteins (15473 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski