Mycobacterium phage Reindeer

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Mclasvirinae; Bongovirus; unclassified Bongovirus

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 140 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G8LI09|A0A7G8LI09_9CAUD DNA helicase OS=Mycobacterium phage Reindeer OX=2762283 GN=71 PE=3 SV=1
MM1 pKa = 7.61IIYY4 pKa = 9.06VAHH7 pKa = 6.9AKK9 pKa = 10.14SDD11 pKa = 3.67SGDD14 pKa = 3.25DD15 pKa = 3.65YY16 pKa = 11.75YY17 pKa = 11.44FVGKK21 pKa = 10.33SEE23 pKa = 4.99AEE25 pKa = 4.19VNTQILDD32 pKa = 3.6VARR35 pKa = 11.84LGWGDD40 pKa = 4.01DD41 pKa = 4.02HH42 pKa = 7.56PDD44 pKa = 3.64FAPDD48 pKa = 3.56PDD50 pKa = 4.33YY51 pKa = 11.49GIHH54 pKa = 7.01TYY56 pKa = 11.24DD57 pKa = 4.12DD58 pKa = 3.38WLMDD62 pKa = 3.97AFQVHH67 pKa = 5.41MEE69 pKa = 3.99KK70 pKa = 10.95FEE72 pKa = 4.12LL73 pKa = 4.33

Molecular weight: 8.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G8LI57|A0A7G8LI57_9CAUD Membrane protein OS=Mycobacterium phage Reindeer OX=2762283 GN=138 PE=4 SV=1
MM1 pKa = 7.69ALALLKK7 pKa = 10.53APRR10 pKa = 11.84VTMPAHH16 pKa = 6.89VYY18 pKa = 8.81QVHH21 pKa = 6.81RR22 pKa = 11.84PTCPNPWKK30 pKa = 9.67TRR32 pKa = 11.84FATAAEE38 pKa = 4.03AHH40 pKa = 6.18RR41 pKa = 11.84VSMDD45 pKa = 2.74ILAKK49 pKa = 10.38NKK51 pKa = 9.48RR52 pKa = 11.84AKK54 pKa = 10.32KK55 pKa = 9.88NRR57 pKa = 11.84TPQGVYY63 pKa = 8.94EE64 pKa = 4.24CRR66 pKa = 11.84CGGFHH71 pKa = 7.37LSDD74 pKa = 3.16ARR76 pKa = 11.84RR77 pKa = 11.84VVVLGGG83 pKa = 3.31

Molecular weight: 9.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

140

0

140

23530

35

2267

168.1

18.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.11 ± 0.59

1.003 ± 0.136

6.613 ± 0.227

6.383 ± 0.282

3.149 ± 0.116

8.381 ± 0.443

2.15 ± 0.209

4.836 ± 0.161

4.144 ± 0.2

7.973 ± 0.18

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.516 ± 0.153

3.536 ± 0.154

5.045 ± 0.244

3.753 ± 0.204

6.498 ± 0.256

5.325 ± 0.152

6.09 ± 0.191

7.212 ± 0.233

2.329 ± 0.147

2.954 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski