Amino acid dipepetide frequency for Mycobacterium phage Reindeer

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.858AlaAla: 11.858 ± 1.587
0.85AlaCys: 0.85 ± 0.206
6.843AlaAsp: 6.843 ± 0.554
7.735AlaGlu: 7.735 ± 0.585
2.805AlaPhe: 2.805 ± 0.361
7.013AlaGly: 7.013 ± 0.761
2.083AlaHis: 2.083 ± 0.308
5.355AlaIle: 5.355 ± 0.434
4.633AlaLys: 4.633 ± 0.444
9.393AlaLeu: 9.393 ± 1.012
2.635AlaMet: 2.635 ± 0.309
3.315AlaAsn: 3.315 ± 0.479
4.335AlaPro: 4.335 ± 0.538
4.505AlaGln: 4.505 ± 0.523
5.398AlaArg: 5.398 ± 0.504
4.93AlaSer: 4.93 ± 0.416
5.525AlaThr: 5.525 ± 0.454
7.395AlaVal: 7.395 ± 0.511
1.998AlaTrp: 1.998 ± 0.278
2.508AlaTyr: 2.508 ± 0.287
0.0AlaXaa: 0.0 ± 0.0
Cys
0.893CysAla: 0.893 ± 0.192
0.085CysCys: 0.085 ± 0.062
0.553CysAsp: 0.553 ± 0.166
0.383CysGlu: 0.383 ± 0.135
0.17CysPhe: 0.17 ± 0.088
1.275CysGly: 1.275 ± 0.317
0.34CysHis: 0.34 ± 0.118
0.723CysIle: 0.723 ± 0.173
0.213CysLys: 0.213 ± 0.102
0.68CysLeu: 0.68 ± 0.209
0.298CysMet: 0.298 ± 0.121
0.298CysAsn: 0.298 ± 0.095
0.765CysPro: 0.765 ± 0.181
0.255CysGln: 0.255 ± 0.097
0.553CysArg: 0.553 ± 0.15
0.68CysSer: 0.68 ± 0.173
0.425CysThr: 0.425 ± 0.12
0.893CysVal: 0.893 ± 0.212
0.383CysTrp: 0.383 ± 0.124
0.17CysTyr: 0.17 ± 0.083
0.0CysXaa: 0.0 ± 0.0
Asp
6.418AspAla: 6.418 ± 0.568
0.978AspCys: 0.978 ± 0.263
4.888AspAsp: 4.888 ± 0.559
5.015AspGlu: 5.015 ± 0.604
1.955AspPhe: 1.955 ± 0.272
6.928AspGly: 6.928 ± 0.553
1.445AspHis: 1.445 ± 0.234
2.763AspIle: 2.763 ± 0.317
2.04AspLys: 2.04 ± 0.248
5.653AspLeu: 5.653 ± 0.482
1.87AspMet: 1.87 ± 0.333
1.828AspAsn: 1.828 ± 0.231
4.08AspPro: 4.08 ± 0.337
2.508AspGln: 2.508 ± 0.351
4.293AspArg: 4.293 ± 0.445
2.805AspSer: 2.805 ± 0.353
2.848AspThr: 2.848 ± 0.333
4.165AspVal: 4.165 ± 0.537
1.658AspTrp: 1.658 ± 0.315
1.998AspTyr: 1.998 ± 0.234
0.0AspXaa: 0.0 ± 0.0
Glu
6.545GluAla: 6.545 ± 0.537
0.553GluCys: 0.553 ± 0.172
4.505GluAsp: 4.505 ± 0.558
3.91GluGlu: 3.91 ± 0.474
2.04GluPhe: 2.04 ± 0.292
4.548GluGly: 4.548 ± 0.388
1.19GluHis: 1.19 ± 0.194
3.613GluIle: 3.613 ± 0.5
2.635GluLys: 2.635 ± 0.312
5.78GluLeu: 5.78 ± 0.463
1.743GluMet: 1.743 ± 0.25
2.125GluAsn: 2.125 ± 0.271
2.89GluPro: 2.89 ± 0.439
2.593GluGln: 2.593 ± 0.307
4.505GluArg: 4.505 ± 0.394
3.528GluSer: 3.528 ± 0.388
3.188GluThr: 3.188 ± 0.335
4.25GluVal: 4.25 ± 0.446
2.04GluTrp: 2.04 ± 0.338
1.658GluTyr: 1.658 ± 0.241
0.0GluXaa: 0.0 ± 0.0
Phe
3.358PheAla: 3.358 ± 0.373
0.255PheCys: 0.255 ± 0.108
2.975PheAsp: 2.975 ± 0.35
1.998PheGlu: 1.998 ± 0.252
0.978PhePhe: 0.978 ± 0.193
2.295PheGly: 2.295 ± 0.333
0.68PheHis: 0.68 ± 0.152
1.7PheIle: 1.7 ± 0.238
1.658PheLys: 1.658 ± 0.271
2.125PheLeu: 2.125 ± 0.334
0.638PheMet: 0.638 ± 0.153
1.445PheAsn: 1.445 ± 0.235
1.445PhePro: 1.445 ± 0.267
1.148PheGln: 1.148 ± 0.216
1.785PheArg: 1.785 ± 0.263
1.488PheSer: 1.488 ± 0.218
1.785PheThr: 1.785 ± 0.237
2.21PheVal: 2.21 ± 0.311
0.68PheTrp: 0.68 ± 0.169
0.85PheTyr: 0.85 ± 0.183
0.0PheXaa: 0.0 ± 0.0
Gly
6.97GlyAla: 6.97 ± 0.741
1.063GlyCys: 1.063 ± 0.254
5.143GlyAsp: 5.143 ± 0.562
4.718GlyGlu: 4.718 ± 0.367
3.103GlyPhe: 3.103 ± 0.363
9.52GlyGly: 9.52 ± 1.339
1.403GlyHis: 1.403 ± 0.253
3.188GlyIle: 3.188 ± 0.414
3.485GlyLys: 3.485 ± 0.441
5.908GlyLeu: 5.908 ± 0.685
1.7GlyMet: 1.7 ± 0.31
3.315GlyAsn: 3.315 ± 0.36
3.698GlyPro: 3.698 ± 0.586
3.103GlyGln: 3.103 ± 0.541
4.803GlyArg: 4.803 ± 0.513
4.803GlySer: 4.803 ± 0.477
5.908GlyThr: 5.908 ± 0.458
6.97GlyVal: 6.97 ± 0.697
2.168GlyTrp: 2.168 ± 0.297
2.805GlyTyr: 2.805 ± 0.397
0.0GlyXaa: 0.0 ± 0.0
His
1.785HisAla: 1.785 ± 0.337
0.34HisCys: 0.34 ± 0.134
1.318HisAsp: 1.318 ± 0.226
1.403HisGlu: 1.403 ± 0.249
0.553HisPhe: 0.553 ± 0.14
1.488HisGly: 1.488 ± 0.227
0.68HisHis: 0.68 ± 0.149
1.7HisIle: 1.7 ± 0.265
0.723HisLys: 0.723 ± 0.171
1.488HisLeu: 1.488 ± 0.265
0.51HisMet: 0.51 ± 0.156
0.893HisAsn: 0.893 ± 0.177
1.105HisPro: 1.105 ± 0.291
0.51HisGln: 0.51 ± 0.131
1.615HisArg: 1.615 ± 0.322
0.85HisSer: 0.85 ± 0.223
1.573HisThr: 1.573 ± 0.263
1.7HisVal: 1.7 ± 0.258
0.51HisTrp: 0.51 ± 0.15
0.765HisTyr: 0.765 ± 0.173
0.0HisXaa: 0.0 ± 0.0
Ile
4.718IleAla: 4.718 ± 0.422
0.34IleCys: 0.34 ± 0.123
4.378IleAsp: 4.378 ± 0.384
3.613IleGlu: 3.613 ± 0.335
1.063IlePhe: 1.063 ± 0.211
3.825IleGly: 3.825 ± 0.414
1.233IleHis: 1.233 ± 0.238
2.253IleIle: 2.253 ± 0.318
2.21IleLys: 2.21 ± 0.27
3.528IleLeu: 3.528 ± 0.387
1.148IleMet: 1.148 ± 0.247
1.785IleAsn: 1.785 ± 0.295
3.4IlePro: 3.4 ± 0.422
1.53IleGln: 1.53 ± 0.24
3.273IleArg: 3.273 ± 0.326
2.083IleSer: 2.083 ± 0.302
3.315IleThr: 3.315 ± 0.346
2.763IleVal: 2.763 ± 0.242
0.85IleTrp: 0.85 ± 0.177
1.063IleTyr: 1.063 ± 0.239
0.0IleXaa: 0.0 ± 0.0
Lys
5.058LysAla: 5.058 ± 0.546
0.298LysCys: 0.298 ± 0.112
1.955LysAsp: 1.955 ± 0.273
1.87LysGlu: 1.87 ± 0.342
1.53LysPhe: 1.53 ± 0.322
2.933LysGly: 2.933 ± 0.423
1.02LysHis: 1.02 ± 0.196
1.658LysIle: 1.658 ± 0.249
1.615LysLys: 1.615 ± 0.298
3.485LysLeu: 3.485 ± 0.354
0.935LysMet: 0.935 ± 0.217
1.87LysAsn: 1.87 ± 0.309
1.7LysPro: 1.7 ± 0.299
1.573LysGln: 1.573 ± 0.25
2.805LysArg: 2.805 ± 0.321
2.295LysSer: 2.295 ± 0.305
2.465LysThr: 2.465 ± 0.331
3.4LysVal: 3.4 ± 0.391
1.403LysTrp: 1.403 ± 0.175
1.573LysTyr: 1.573 ± 0.298
0.0LysXaa: 0.0 ± 0.0
Leu
9.223LeuAla: 9.223 ± 0.627
0.553LeuCys: 0.553 ± 0.185
5.525LeuAsp: 5.525 ± 0.451
4.633LeuGlu: 4.633 ± 0.456
2.423LeuPhe: 2.423 ± 0.303
6.63LeuGly: 6.63 ± 0.484
1.318LeuHis: 1.318 ± 0.219
3.783LeuIle: 3.783 ± 0.466
3.485LeuLys: 3.485 ± 0.375
5.78LeuLeu: 5.78 ± 0.438
1.998LeuMet: 1.998 ± 0.292
2.72LeuAsn: 2.72 ± 0.457
4.123LeuPro: 4.123 ± 0.502
3.315LeuGln: 3.315 ± 0.407
5.483LeuArg: 5.483 ± 0.43
4.293LeuSer: 4.293 ± 0.448
5.143LeuThr: 5.143 ± 0.468
5.483LeuVal: 5.483 ± 0.523
1.233LeuTrp: 1.233 ± 0.238
2.593LeuTyr: 2.593 ± 0.397
0.0LeuXaa: 0.0 ± 0.0
Met
3.698MetAla: 3.698 ± 0.339
0.17MetCys: 0.17 ± 0.083
1.233MetAsp: 1.233 ± 0.225
1.36MetGlu: 1.36 ± 0.202
0.553MetPhe: 0.553 ± 0.152
1.53MetGly: 1.53 ± 0.254
0.425MetHis: 0.425 ± 0.146
0.935MetIle: 0.935 ± 0.176
0.765MetLys: 0.765 ± 0.174
1.445MetLeu: 1.445 ± 0.274
0.468MetMet: 0.468 ± 0.132
0.68MetAsn: 0.68 ± 0.152
1.445MetPro: 1.445 ± 0.21
0.893MetGln: 0.893 ± 0.216
1.743MetArg: 1.743 ± 0.307
2.89MetSer: 2.89 ± 0.366
2.423MetThr: 2.423 ± 0.286
1.318MetVal: 1.318 ± 0.195
0.553MetTrp: 0.553 ± 0.161
0.638MetTyr: 0.638 ± 0.201
0.0MetXaa: 0.0 ± 0.0
Asn
3.4AsnAla: 3.4 ± 0.382
0.298AsnCys: 0.298 ± 0.111
1.36AsnAsp: 1.36 ± 0.228
1.913AsnGlu: 1.913 ± 0.24
1.318AsnPhe: 1.318 ± 0.23
3.825AsnGly: 3.825 ± 0.394
0.723AsnHis: 0.723 ± 0.145
1.7AsnIle: 1.7 ± 0.235
1.275AsnLys: 1.275 ± 0.255
2.975AsnLeu: 2.975 ± 0.323
0.765AsnMet: 0.765 ± 0.154
1.148AsnAsn: 1.148 ± 0.23
2.763AsnPro: 2.763 ± 0.331
1.275AsnGln: 1.275 ± 0.242
2.805AsnArg: 2.805 ± 0.325
1.87AsnSer: 1.87 ± 0.244
2.04AsnThr: 2.04 ± 0.267
2.338AsnVal: 2.338 ± 0.271
0.638AsnTrp: 0.638 ± 0.154
0.935AsnTyr: 0.935 ± 0.198
0.0AsnXaa: 0.0 ± 0.0
Pro
5.058ProAla: 5.058 ± 0.818
0.34ProCys: 0.34 ± 0.105
3.315ProAsp: 3.315 ± 0.382
3.783ProGlu: 3.783 ± 0.504
1.743ProPhe: 1.743 ± 0.259
5.015ProGly: 5.015 ± 0.688
0.978ProHis: 0.978 ± 0.209
1.743ProIle: 1.743 ± 0.304
2.04ProLys: 2.04 ± 0.345
3.783ProLeu: 3.783 ± 0.388
1.743ProMet: 1.743 ± 0.24
1.87ProAsn: 1.87 ± 0.31
2.465ProPro: 2.465 ± 0.362
1.53ProGln: 1.53 ± 0.276
2.678ProArg: 2.678 ± 0.413
2.465ProSer: 2.465 ± 0.306
3.188ProThr: 3.188 ± 0.334
3.995ProVal: 3.995 ± 0.343
0.978ProTrp: 0.978 ± 0.225
1.743ProTyr: 1.743 ± 0.278
0.0ProXaa: 0.0 ± 0.0
Gln
3.783GlnAla: 3.783 ± 0.506
0.51GlnCys: 0.51 ± 0.167
1.743GlnAsp: 1.743 ± 0.196
1.615GlnGlu: 1.615 ± 0.224
0.85GlnPhe: 0.85 ± 0.198
2.933GlnGly: 2.933 ± 0.384
0.765GlnHis: 0.765 ± 0.174
2.593GlnIle: 2.593 ± 0.344
1.573GlnLys: 1.573 ± 0.238
3.57GlnLeu: 3.57 ± 0.355
0.723GlnMet: 0.723 ± 0.19
1.233GlnAsn: 1.233 ± 0.248
1.87GlnPro: 1.87 ± 0.31
2.125GlnGln: 2.125 ± 0.319
2.593GlnArg: 2.593 ± 0.325
1.488GlnSer: 1.488 ± 0.231
2.338GlnThr: 2.338 ± 0.332
2.89GlnVal: 2.89 ± 0.392
1.403GlnTrp: 1.403 ± 0.224
0.935GlnTyr: 0.935 ± 0.214
0.0GlnXaa: 0.0 ± 0.0
Arg
6.29ArgAla: 6.29 ± 0.495
0.68ArgCys: 0.68 ± 0.17
3.655ArgAsp: 3.655 ± 0.384
4.208ArgGlu: 4.208 ± 0.402
2.423ArgPhe: 2.423 ± 0.332
4.59ArgGly: 4.59 ± 0.435
0.893ArgHis: 0.893 ± 0.186
4.165ArgIle: 4.165 ± 0.394
3.273ArgLys: 3.273 ± 0.428
5.1ArgLeu: 5.1 ± 0.492
2.083ArgMet: 2.083 ± 0.323
2.635ArgAsn: 2.635 ± 0.292
2.848ArgPro: 2.848 ± 0.362
2.89ArgGln: 2.89 ± 0.297
4.548ArgArg: 4.548 ± 0.529
3.103ArgSer: 3.103 ± 0.312
3.188ArgThr: 3.188 ± 0.341
4.888ArgVal: 4.888 ± 0.364
1.488ArgTrp: 1.488 ± 0.24
2.04ArgTyr: 2.04 ± 0.282
0.0ArgXaa: 0.0 ± 0.0
Ser
4.718SerAla: 4.718 ± 0.527
0.553SerCys: 0.553 ± 0.178
4.208SerAsp: 4.208 ± 0.46
2.763SerGlu: 2.763 ± 0.382
1.955SerPhe: 1.955 ± 0.271
4.59SerGly: 4.59 ± 0.404
1.02SerHis: 1.02 ± 0.205
2.168SerIle: 2.168 ± 0.287
1.955SerLys: 1.955 ± 0.283
3.953SerLeu: 3.953 ± 0.389
1.658SerMet: 1.658 ± 0.311
2.38SerAsn: 2.38 ± 0.323
2.253SerPro: 2.253 ± 0.241
1.658SerGln: 1.658 ± 0.268
3.57SerArg: 3.57 ± 0.345
3.358SerSer: 3.358 ± 0.463
3.91SerThr: 3.91 ± 0.361
3.528SerVal: 3.528 ± 0.443
1.318SerTrp: 1.318 ± 0.236
1.743SerTyr: 1.743 ± 0.215
0.0SerXaa: 0.0 ± 0.0
Thr
6.078ThrAla: 6.078 ± 0.488
0.595ThrCys: 0.595 ± 0.198
3.91ThrAsp: 3.91 ± 0.454
3.953ThrGlu: 3.953 ± 0.351
2.083ThrPhe: 2.083 ± 0.326
5.483ThrGly: 5.483 ± 0.537
1.445ThrHis: 1.445 ± 0.259
3.103ThrIle: 3.103 ± 0.44
2.508ThrLys: 2.508 ± 0.377
4.675ThrLeu: 4.675 ± 0.407
1.318ThrMet: 1.318 ± 0.213
1.785ThrAsn: 1.785 ± 0.236
3.655ThrPro: 3.655 ± 0.459
1.743ThrGln: 1.743 ± 0.242
3.57ThrArg: 3.57 ± 0.405
2.55ThrSer: 2.55 ± 0.365
3.4ThrThr: 3.4 ± 0.344
5.61ThrVal: 5.61 ± 0.568
1.403ThrTrp: 1.403 ± 0.259
2.04ThrTyr: 2.04 ± 0.307
0.0ThrXaa: 0.0 ± 0.0
Val
6.758ValAla: 6.758 ± 0.635
0.85ValCys: 0.85 ± 0.277
5.313ValAsp: 5.313 ± 0.563
4.973ValGlu: 4.973 ± 0.505
2.168ValPhe: 2.168 ± 0.26
5.993ValGly: 5.993 ± 0.626
2.423ValHis: 2.423 ± 0.354
3.145ValIle: 3.145 ± 0.376
3.103ValLys: 3.103 ± 0.316
6.078ValLeu: 6.078 ± 0.509
1.148ValMet: 1.148 ± 0.323
2.21ValAsn: 2.21 ± 0.291
3.358ValPro: 3.358 ± 0.44
2.72ValGln: 2.72 ± 0.378
4.293ValArg: 4.293 ± 0.411
4.888ValSer: 4.888 ± 0.5
4.675ValThr: 4.675 ± 0.671
4.888ValVal: 4.888 ± 0.444
1.36ValTrp: 1.36 ± 0.255
1.785ValTyr: 1.785 ± 0.292
0.0ValXaa: 0.0 ± 0.0
Trp
1.913TrpAla: 1.913 ± 0.245
0.425TrpCys: 0.425 ± 0.146
1.36TrpAsp: 1.36 ± 0.234
1.998TrpGlu: 1.998 ± 0.254
0.808TrpPhe: 0.808 ± 0.213
1.403TrpGly: 1.403 ± 0.266
0.723TrpHis: 0.723 ± 0.201
0.808TrpIle: 0.808 ± 0.146
0.935TrpLys: 0.935 ± 0.158
2.21TrpLeu: 2.21 ± 0.361
0.978TrpMet: 0.978 ± 0.178
0.553TrpAsn: 0.553 ± 0.138
0.935TrpPro: 0.935 ± 0.178
0.723TrpGln: 0.723 ± 0.163
1.87TrpArg: 1.87 ± 0.257
1.318TrpSer: 1.318 ± 0.258
1.403TrpThr: 1.403 ± 0.224
1.615TrpVal: 1.615 ± 0.283
0.68TrpTrp: 0.68 ± 0.176
0.638TrpTyr: 0.638 ± 0.157
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.593TyrAla: 2.593 ± 0.341
0.298TyrCys: 0.298 ± 0.114
1.955TyrAsp: 1.955 ± 0.268
1.998TyrGlu: 1.998 ± 0.287
0.978TyrPhe: 0.978 ± 0.182
1.998TyrGly: 1.998 ± 0.276
0.723TyrHis: 0.723 ± 0.155
1.275TyrIle: 1.275 ± 0.21
1.318TyrLys: 1.318 ± 0.171
2.125TyrLeu: 2.125 ± 0.26
0.765TyrMet: 0.765 ± 0.19
1.275TyrAsn: 1.275 ± 0.247
1.318TyrPro: 1.318 ± 0.288
0.935TyrGln: 0.935 ± 0.231
2.805TyrArg: 2.805 ± 0.337
1.573TyrSer: 1.573 ± 0.233
2.168TyrThr: 2.168 ± 0.381
1.828TyrVal: 1.828 ± 0.232
0.553TyrTrp: 0.553 ± 0.13
1.063TyrTyr: 1.063 ± 0.211
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 140 proteins (23530 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski