Varibaculum cambriense DORA_20

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Varibaculum; Varibaculum cambriense

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2003 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W1TWR6|W1TWR6_9ACTO PKS_ER domain-containing protein OS=Varibaculum cambriense DORA_20 OX=1403948 GN=Q618_VCMC00001G1334 PE=4 SV=1
MM1 pKa = 7.66TYY3 pKa = 10.9DD4 pKa = 3.46EE5 pKa = 5.75LYY7 pKa = 7.87VTSRR11 pKa = 11.84AFFEE15 pKa = 4.33AHH17 pKa = 6.02ATPEE21 pKa = 3.95QIAFLDD27 pKa = 3.73SHH29 pKa = 6.35EE30 pKa = 4.67KK31 pKa = 10.42CDD33 pKa = 3.54GAGFVDD39 pKa = 5.14SILFFIDD46 pKa = 4.76LFLGDD51 pKa = 3.98MPGVPEE57 pKa = 5.99KK58 pKa = 10.56LANWQASLDD67 pKa = 3.82ALPEE71 pKa = 3.85YY72 pKa = 10.64DD73 pKa = 3.64

Molecular weight: 8.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W1TTC0|W1TTC0_9ACTO Probable nicotinate-nucleotide adenylyltransferase OS=Varibaculum cambriense DORA_20 OX=1403948 GN=nadD PE=3 SV=1
MM1 pKa = 7.4SRR3 pKa = 11.84TFTSFKK9 pKa = 8.25YY10 pKa = 10.34HH11 pKa = 7.13NYY13 pKa = 9.06RR14 pKa = 11.84LWWMSNIFASTATWMQRR31 pKa = 11.84VAQDD35 pKa = 3.21WVVLMVLTDD44 pKa = 3.3QSGFAVGVVTALQFLPQLLLSIPAGMVADD73 pKa = 4.45RR74 pKa = 11.84FNRR77 pKa = 11.84RR78 pKa = 11.84RR79 pKa = 11.84IIQICQCIVALCGLLTGILLLTGVAALWHH108 pKa = 6.62IYY110 pKa = 9.81IIALITGVADD120 pKa = 4.1CLTSPARR127 pKa = 11.84QAFVSEE133 pKa = 4.57LVTKK137 pKa = 10.45EE138 pKa = 4.19DD139 pKa = 3.49LPNAVGLNSTAFNSARR155 pKa = 11.84LIGPGLAGFVIAGIGPGWVFIANFVLFIVPVLGLSLMDD193 pKa = 3.94PARR196 pKa = 11.84LYY198 pKa = 10.82RR199 pKa = 11.84PEE201 pKa = 3.83KK202 pKa = 8.86TSRR205 pKa = 11.84TKK207 pKa = 11.1GMMRR211 pKa = 11.84EE212 pKa = 3.97GLRR215 pKa = 11.84YY216 pKa = 8.65VQNRR220 pKa = 11.84TDD222 pKa = 2.95IKK224 pKa = 10.94AIIAILTVVGALGLNFQLTQAVMATRR250 pKa = 11.84VFGKK254 pKa = 10.62GAGEE258 pKa = 3.92YY259 pKa = 10.51GLLGSIMAIGALAGALQAARR279 pKa = 11.84RR280 pKa = 11.84RR281 pKa = 11.84QPRR284 pKa = 11.84VFTVVVSAVLFGVLEE299 pKa = 4.4GALALAPTYY308 pKa = 10.36EE309 pKa = 4.09IFALLLIPTGFVMLNLLTSANATIQVSTEE338 pKa = 3.55EE339 pKa = 4.32EE340 pKa = 3.49IRR342 pKa = 11.84GRR344 pKa = 11.84VLSIYY349 pKa = 10.44FLFFLGTTPIGAPIIGWVAEE369 pKa = 3.96HH370 pKa = 6.94WGPRR374 pKa = 11.84WSLGAGAIASLLVALIVGIWLWRR397 pKa = 11.84HH398 pKa = 4.39WKK400 pKa = 10.32VDD402 pKa = 3.28VTLRR406 pKa = 11.84ASRR409 pKa = 11.84PFVSIEE415 pKa = 3.96GPRR418 pKa = 11.84EE419 pKa = 3.18RR420 pKa = 11.84AMRR423 pKa = 11.84RR424 pKa = 11.84RR425 pKa = 11.84KK426 pKa = 9.37EE427 pKa = 3.49RR428 pKa = 11.84AEE430 pKa = 3.9RR431 pKa = 11.84QRR433 pKa = 11.84IMDD436 pKa = 3.72NQSRR440 pKa = 11.84DD441 pKa = 3.12TGTT444 pKa = 3.0

Molecular weight: 48.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2003

0

2003

657404

26

2987

328.2

35.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.841 ± 0.066

0.956 ± 0.02

5.376 ± 0.043

6.106 ± 0.06

3.249 ± 0.034

8.017 ± 0.047

1.787 ± 0.026

5.324 ± 0.047

4.578 ± 0.049

9.785 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.16 ± 0.03

3.181 ± 0.039

5.03 ± 0.04

4.203 ± 0.036

5.82 ± 0.056

6.365 ± 0.045

5.745 ± 0.051

7.373 ± 0.045

1.385 ± 0.027

2.528 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski