Amino acid dipepetide frequency for Varibaculum cambriense DORA_20

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.238AlaAla: 13.238 ± 0.226	1.112AlaCys: 1.112 ± 0.043	6.039AlaAsp: 6.039 ± 0.093	6.371AlaGlu: 6.371 ± 0.121	3.239AlaPhe: 3.239 ± 0.087	9.702AlaGly: 9.702 ± 0.146	1.869AlaHis: 1.869 ± 0.055	5.537AlaIle: 5.537 ± 0.114	5.249AlaLys: 5.249 ± 0.104	11.168AlaLeu: 11.168 ± 0.172	2.288AlaMet: 2.288 ± 0.063	3.252AlaAsn: 3.252 ± 0.083	4.6AlaPro: 4.6 ± 0.103	4.906AlaGln: 4.906 ± 0.108	6.304AlaArg: 6.304 ± 0.131	6.434AlaSer: 6.434 ± 0.107	5.738AlaThr: 5.738 ± 0.106	7.58AlaVal: 7.58 ± 0.13	1.392AlaTrp: 1.392 ± 0.056	2.355AlaTyr: 2.355 ± 0.058	0.041AlaXaa: 0.041 ± 0.007
Cys	1.139CysAla: 1.139 ± 0.045	0.116CysCys: 0.116 ± 0.016	0.502CysAsp: 0.502 ± 0.028	0.538CysGlu: 0.538 ± 0.027	0.357CysPhe: 0.357 ± 0.024	1.01CysGly: 1.01 ± 0.036	0.234CysHis: 0.234 ± 0.02	0.38CysIle: 0.38 ± 0.025	0.298CysLys: 0.298 ± 0.021	0.958CysLeu: 0.958 ± 0.044	0.186CysMet: 0.186 ± 0.018	0.249CysAsn: 0.249 ± 0.02	0.598CysPro: 0.598 ± 0.032	0.371CysGln: 0.371 ± 0.026	0.552CysArg: 0.552 ± 0.031	0.648CysSer: 0.648 ± 0.029	0.487CysThr: 0.487 ± 0.028	0.546CysVal: 0.546 ± 0.03	0.137CysTrp: 0.137 ± 0.018	0.254CysTyr: 0.254 ± 0.02	0.0CysXaa: 0.0 ± 0.0
Asp	5.208AspAla: 5.208 ± 0.092	0.488AspCys: 0.488 ± 0.025	2.364AspAsp: 2.364 ± 0.072	3.383AspGlu: 3.383 ± 0.096	2.128AspPhe: 2.128 ± 0.067	4.048AspGly: 4.048 ± 0.096	1.007AspHis: 1.007 ± 0.042	2.718AspIle: 2.718 ± 0.068	2.508AspLys: 2.508 ± 0.089	5.873AspLeu: 5.873 ± 0.111	1.126AspMet: 1.126 ± 0.045	1.695AspAsn: 1.695 ± 0.061	3.526AspPro: 3.526 ± 0.08	2.478AspGln: 2.478 ± 0.067	2.919AspArg: 2.919 ± 0.071	3.518AspSer: 3.518 ± 0.074	2.969AspThr: 2.969 ± 0.07	3.199AspVal: 3.199 ± 0.072	0.878AspTrp: 0.878 ± 0.044	1.714AspTyr: 1.714 ± 0.062	0.008AspXaa: 0.008 ± 0.003
Glu	6.199GluAla: 6.199 ± 0.137	0.406GluCys: 0.406 ± 0.025	3.601GluAsp: 3.601 ± 0.097	4.224GluGlu: 4.224 ± 0.097	1.725GluPhe: 1.725 ± 0.055	4.008GluGly: 4.008 ± 0.08	1.063GluHis: 1.063 ± 0.046	3.949GluIle: 3.949 ± 0.086	3.62GluLys: 3.62 ± 0.084	5.919GluLeu: 5.919 ± 0.104	1.374GluMet: 1.374 ± 0.045	2.604GluAsn: 2.604 ± 0.061	2.431GluPro: 2.431 ± 0.066	2.974GluGln: 2.974 ± 0.073	3.543GluArg: 3.543 ± 0.069	3.106GluSer: 3.106 ± 0.073	3.333GluThr: 3.333 ± 0.072	4.884GluVal: 4.884 ± 0.102	0.611GluTrp: 0.611 ± 0.031	1.48GluTyr: 1.48 ± 0.058	0.008GluXaa: 0.008 ± 0.003
Phe	3.395PheAla: 3.395 ± 0.081	0.399PheCys: 0.399 ± 0.025	1.939PheAsp: 1.939 ± 0.056	1.812PheGlu: 1.812 ± 0.061	1.29PhePhe: 1.29 ± 0.052	2.787PheGly: 2.787 ± 0.077	0.558PheHis: 0.558 ± 0.028	1.62PheIle: 1.62 ± 0.056	1.226PheLys: 1.226 ± 0.052	3.045PheLeu: 3.045 ± 0.073	0.601PheMet: 0.601 ± 0.03	1.037PheAsn: 1.037 ± 0.041	1.504PhePro: 1.504 ± 0.049	1.13PheGln: 1.13 ± 0.042	1.606PheArg: 1.606 ± 0.053	2.674PheSer: 2.674 ± 0.061	2.358PheThr: 2.358 ± 0.079	2.149PheVal: 2.149 ± 0.057	0.478PheTrp: 0.478 ± 0.029	0.876PheTyr: 0.876 ± 0.03	0.005PheXaa: 0.005 ± 0.003
Gly	8.056GlyAla: 8.056 ± 0.119	0.796GlyCys: 0.796 ± 0.034	3.987GlyAsp: 3.987 ± 0.096	4.947GlyGlu: 4.947 ± 0.098	2.899GlyPhe: 2.899 ± 0.074	6.206GlyGly: 6.206 ± 0.129	1.424GlyHis: 1.424 ± 0.049	5.099GlyIle: 5.099 ± 0.105	5.196GlyLys: 5.196 ± 0.139	7.193GlyLeu: 7.193 ± 0.119	2.064GlyMet: 2.064 ± 0.061	2.68GlyAsn: 2.68 ± 0.064	2.335GlyPro: 2.335 ± 0.061	3.441GlyGln: 3.441 ± 0.084	4.423GlyArg: 4.423 ± 0.095	4.997GlySer: 4.997 ± 0.096	4.807GlyThr: 4.807 ± 0.11	5.904GlyVal: 5.904 ± 0.103	1.266GlyTrp: 1.266 ± 0.052	2.414GlyTyr: 2.414 ± 0.061	0.035GlyXaa: 0.035 ± 0.008
His	1.637HisAla: 1.637 ± 0.049	0.192HisCys: 0.192 ± 0.017	0.937HisAsp: 0.937 ± 0.039	0.963HisGlu: 0.963 ± 0.039	0.584HisPhe: 0.584 ± 0.026	1.378HisGly: 1.378 ± 0.051	0.482HisHis: 0.482 ± 0.028	0.817HisIle: 0.817 ± 0.035	0.54HisLys: 0.54 ± 0.027	2.061HisLeu: 2.061 ± 0.056	0.386HisMet: 0.386 ± 0.023	0.532HisAsn: 0.532 ± 0.031	1.354HisPro: 1.354 ± 0.048	0.799HisGln: 0.799 ± 0.034	1.19HisArg: 1.19 ± 0.05	1.123HisSer: 1.123 ± 0.037	0.972HisThr: 0.972 ± 0.042	1.153HisVal: 1.153 ± 0.043	0.274HisTrp: 0.274 ± 0.021	0.499HisTyr: 0.499 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	6.231IleAla: 6.231 ± 0.102	0.747IleCys: 0.747 ± 0.036	3.184IleAsp: 3.184 ± 0.059	3.185IleGlu: 3.185 ± 0.073	1.783IlePhe: 1.783 ± 0.063	4.493IleGly: 4.493 ± 0.095	0.891IleHis: 0.891 ± 0.039	2.714IleIle: 2.714 ± 0.086	2.187IleLys: 2.187 ± 0.063	4.475IleLeu: 4.475 ± 0.107	1.024IleMet: 1.024 ± 0.04	1.828IleAsn: 1.828 ± 0.056	3.033IlePro: 3.033 ± 0.07	1.378IleGln: 1.378 ± 0.048	2.677IleArg: 2.677 ± 0.065	3.921IleSer: 3.921 ± 0.091	3.398IleThr: 3.398 ± 0.08	4.042IleVal: 4.042 ± 0.088	0.677IleTrp: 0.677 ± 0.035	1.368IleTyr: 1.368 ± 0.049	0.002IleXaa: 0.002 ± 0.002
Lys	4.859LysAla: 4.859 ± 0.136	0.329LysCys: 0.329 ± 0.024	2.619LysAsp: 2.619 ± 0.083	3.013LysGlu: 3.013 ± 0.08	1.156LysPhe: 1.156 ± 0.051	2.937LysGly: 2.937 ± 0.078	0.811LysHis: 0.811 ± 0.037	2.629LysIle: 2.629 ± 0.069	2.429LysLys: 2.429 ± 0.08	4.055LysLeu: 4.055 ± 0.088	1.261LysMet: 1.261 ± 0.048	1.803LysAsn: 1.803 ± 0.063	2.56LysPro: 2.56 ± 0.075	1.988LysGln: 1.988 ± 0.059	2.466LysArg: 2.466 ± 0.065	2.519LysSer: 2.519 ± 0.063	3.03LysThr: 3.03 ± 0.084	3.602LysVal: 3.602 ± 0.091	0.538LysTrp: 0.538 ± 0.027	1.179LysTyr: 1.179 ± 0.052	0.002LysXaa: 0.002 ± 0.001
Leu	12.038LeuAla: 12.038 ± 0.171	0.926LeuCys: 0.926 ± 0.039	5.643LeuAsp: 5.643 ± 0.11	5.887LeuGlu: 5.887 ± 0.117	2.873LeuPhe: 2.873 ± 0.079	8.105LeuGly: 8.105 ± 0.144	1.571LeuHis: 1.571 ± 0.048	4.947LeuIle: 4.947 ± 0.111	4.025LeuLys: 4.025 ± 0.088	9.189LeuLeu: 9.189 ± 0.166	1.882LeuMet: 1.882 ± 0.061	2.676LeuAsn: 2.676 ± 0.061	5.671LeuPro: 5.671 ± 0.094	3.555LeuGln: 3.555 ± 0.083	5.64LeuArg: 5.64 ± 0.114	6.62LeuSer: 6.62 ± 0.108	5.967LeuThr: 5.967 ± 0.106	7.574LeuVal: 7.574 ± 0.139	1.059LeuTrp: 1.059 ± 0.047	1.996LeuTyr: 1.996 ± 0.057	0.006LeuXaa: 0.006 ± 0.003
Met	2.353MetAla: 2.353 ± 0.072	0.173MetCys: 0.173 ± 0.018	1.066MetAsp: 1.066 ± 0.042	1.17MetGlu: 1.17 ± 0.042	0.608MetPhe: 0.608 ± 0.032	1.682MetGly: 1.682 ± 0.055	0.4MetHis: 0.4 ± 0.026	1.147MetIle: 1.147 ± 0.046	0.928MetLys: 0.928 ± 0.042	2.073MetLeu: 2.073 ± 0.066	0.511MetMet: 0.511 ± 0.035	0.814MetAsn: 0.814 ± 0.039	1.098MetPro: 1.098 ± 0.045	0.761MetGln: 0.761 ± 0.034	1.515MetArg: 1.515 ± 0.05	1.669MetSer: 1.669 ± 0.047	1.331MetThr: 1.331 ± 0.045	1.736MetVal: 1.736 ± 0.057	0.198MetTrp: 0.198 ± 0.019	0.37MetTyr: 0.37 ± 0.023	0.002MetXaa: 0.002 ± 0.001
Asn	3.077AsnAla: 3.077 ± 0.073	0.298AsnCys: 0.298 ± 0.018	1.48AsnAsp: 1.48 ± 0.057	1.666AsnGlu: 1.666 ± 0.045	0.913AsnPhe: 0.913 ± 0.041	2.365AsnGly: 2.365 ± 0.086	0.604AsnHis: 0.604 ± 0.034	1.599AsnIle: 1.599 ± 0.048	1.278AsnLys: 1.278 ± 0.054	3.464AsnLeu: 3.464 ± 0.075	0.747AsnMet: 0.747 ± 0.04	1.129AsnAsn: 1.129 ± 0.055	2.586AsnPro: 2.586 ± 0.072	1.6AsnGln: 1.6 ± 0.053	1.851AsnArg: 1.851 ± 0.054	1.994AsnSer: 1.994 ± 0.05	1.71AsnThr: 1.71 ± 0.071	1.946AsnVal: 1.946 ± 0.058	0.578AsnTrp: 0.578 ± 0.028	0.922AsnTyr: 0.922 ± 0.044	0.003AsnXaa: 0.003 ± 0.002
Pro	5.855ProAla: 5.855 ± 0.123	0.335ProCys: 0.335 ± 0.022	2.978ProAsp: 2.978 ± 0.07	3.97ProGlu: 3.97 ± 0.081	1.5ProPhe: 1.5 ± 0.044	4.48ProGly: 4.48 ± 0.09	0.999ProHis: 0.999 ± 0.036	2.256ProIle: 2.256 ± 0.053	2.093ProLys: 2.093 ± 0.063	4.676ProLeu: 4.676 ± 0.09	0.831ProMet: 0.831 ± 0.037	1.526ProAsn: 1.526 ± 0.063	2.081ProPro: 2.081 ± 0.078	2.443ProGln: 2.443 ± 0.074	2.621ProArg: 2.621 ± 0.073	3.007ProSer: 3.007 ± 0.068	2.84ProThr: 2.84 ± 0.071	3.848ProVal: 3.848 ± 0.096	0.662ProTrp: 0.662 ± 0.032	1.291ProTyr: 1.291 ± 0.043	0.012ProXaa: 0.012 ± 0.004
Gln	5.234GlnAla: 5.234 ± 0.094	0.251GlnCys: 0.251 ± 0.018	1.976GlnAsp: 1.976 ± 0.059	2.502GlnGlu: 2.502 ± 0.06	1.063GlnPhe: 1.063 ± 0.042	2.778GlnGly: 2.778 ± 0.066	0.59GlnHis: 0.59 ± 0.029	2.838GlnIle: 2.838 ± 0.071	1.848GlnLys: 1.848 ± 0.067	4.58GlnLeu: 4.58 ± 0.098	1.072GlnMet: 1.072 ± 0.047	1.206GlnAsn: 1.206 ± 0.041	1.787GlnPro: 1.787 ± 0.065	1.813GlnGln: 1.813 ± 0.064	2.467GlnArg: 2.467 ± 0.078	1.968GlnSer: 1.968 ± 0.063	2.169GlnThr: 2.169 ± 0.078	4.355GlnVal: 4.355 ± 0.095	0.59GlnTrp: 0.59 ± 0.034	0.928GlnTyr: 0.928 ± 0.04	0.008GlnXaa: 0.008 ± 0.003
Arg	5.537ArgAla: 5.537 ± 0.103	0.557ArgCys: 0.557 ± 0.031	3.108ArgAsp: 3.108 ± 0.082	4.064ArgGlu: 4.064 ± 0.093	2.05ArgPhe: 2.05 ± 0.057	4.122ArgGly: 4.122 ± 0.092	1.152ArgHis: 1.152 ± 0.045	3.322ArgIle: 3.322 ± 0.07	2.648ArgLys: 2.648 ± 0.071	5.747ArgLeu: 5.747 ± 0.106	1.368ArgMet: 1.368 ± 0.05	1.59ArgAsn: 1.59 ± 0.055	2.689ArgPro: 2.689 ± 0.074	2.787ArgGln: 2.787 ± 0.074	4.395ArgArg: 4.395 ± 0.111	3.532ArgSer: 3.532 ± 0.07	2.858ArgThr: 2.858 ± 0.07	4.087ArgVal: 4.087 ± 0.098	0.869ArgTrp: 0.869 ± 0.037	1.717ArgTyr: 1.717 ± 0.046	0.006ArgXaa: 0.006 ± 0.003
Ser	6.81SerAla: 6.81 ± 0.11	0.552SerCys: 0.552 ± 0.033	3.216SerAsp: 3.216 ± 0.067	3.987SerGlu: 3.987 ± 0.08	2.268SerPhe: 2.268 ± 0.055	6.38SerGly: 6.38 ± 0.111	1.138SerHis: 1.138 ± 0.042	2.662SerIle: 2.662 ± 0.076	2.554SerLys: 2.554 ± 0.066	6.358SerLeu: 6.358 ± 0.1	1.281SerMet: 1.281 ± 0.041	1.72SerAsn: 1.72 ± 0.052	3.292SerPro: 3.292 ± 0.078	2.972SerGln: 2.972 ± 0.071	3.991SerArg: 3.991 ± 0.089	4.493SerSer: 4.493 ± 0.1	3.252SerThr: 3.252 ± 0.061	3.873SerVal: 3.873 ± 0.078	1.135SerTrp: 1.135 ± 0.048	1.71SerTyr: 1.71 ± 0.053	0.011SerXaa: 0.011 ± 0.004
Thr	5.826ThrAla: 5.826 ± 0.102	0.631ThrCys: 0.631 ± 0.035	3.076ThrAsp: 3.076 ± 0.084	2.934ThrGlu: 2.934 ± 0.07	1.941ThrPhe: 1.941 ± 0.067	5.099ThrGly: 5.099 ± 0.101	1.054ThrHis: 1.054 ± 0.042	3.083ThrIle: 3.083 ± 0.068	2.27ThrLys: 2.27 ± 0.076	5.517ThrLeu: 5.517 ± 0.095	1.086ThrMet: 1.086 ± 0.041	1.761ThrAsn: 1.761 ± 0.059	3.59ThrPro: 3.59 ± 0.102	2.2ThrGln: 2.2 ± 0.063	3.1ThrArg: 3.1 ± 0.07	3.716ThrSer: 3.716 ± 0.083	3.296ThrThr: 3.296 ± 0.078	4.82ThrVal: 4.82 ± 0.118	0.945ThrTrp: 0.945 ± 0.04	1.489ThrTyr: 1.489 ± 0.051	0.011ThrXaa: 0.011 ± 0.004
Val	8.238ValAla: 8.238 ± 0.122	0.771ValCys: 0.771 ± 0.039	3.923ValAsp: 3.923 ± 0.092	4.249ValGlu: 4.249 ± 0.091	2.647ValPhe: 2.647 ± 0.07	5.458ValGly: 5.458 ± 0.11	1.214ValHis: 1.214 ± 0.041	4.232ValIle: 4.232 ± 0.089	3.445ValLys: 3.445 ± 0.087	7.087ValLeu: 7.087 ± 0.131	1.681ValMet: 1.681 ± 0.058	2.35ValAsn: 2.35 ± 0.062	3.62ValPro: 3.62 ± 0.078	2.244ValGln: 2.244 ± 0.059	4.046ValArg: 4.046 ± 0.094	5.318ValSer: 5.318 ± 0.09	4.941ValThr: 4.941 ± 0.108	5.745ValVal: 5.745 ± 0.122	0.873ValTrp: 0.873 ± 0.043	1.637ValTyr: 1.637 ± 0.051	0.012ValXaa: 0.012 ± 0.004
Trp	1.234TrpAla: 1.234 ± 0.046	0.154TrpCys: 0.154 ± 0.018	0.788TrpAsp: 0.788 ± 0.035	0.846TrpGlu: 0.846 ± 0.04	0.475TrpPhe: 0.475 ± 0.03	1.063TrpGly: 1.063 ± 0.047	0.301TrpHis: 0.301 ± 0.024	0.747TrpIle: 0.747 ± 0.034	0.636TrpLys: 0.636 ± 0.033	1.447TrpLeu: 1.447 ± 0.053	0.362TrpMet: 0.362 ± 0.026	0.563TrpAsn: 0.563 ± 0.035	0.526TrpPro: 0.526 ± 0.029	0.754TrpGln: 0.754 ± 0.037	0.94TrpArg: 0.94 ± 0.041	0.733TrpSer: 0.733 ± 0.034	0.662TrpThr: 0.662 ± 0.032	1.024TrpVal: 1.024 ± 0.044	0.268TrpTrp: 0.268 ± 0.02	0.324TrpTyr: 0.324 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.227TyrAla: 2.227 ± 0.063	0.329TyrCys: 0.329 ± 0.023	1.323TyrAsp: 1.323 ± 0.055	1.343TyrGlu: 1.343 ± 0.055	0.986TyrPhe: 0.986 ± 0.041	2.049TyrGly: 2.049 ± 0.059	0.503TyrHis: 0.503 ± 0.03	0.937TyrIle: 0.937 ± 0.04	0.799TyrLys: 0.799 ± 0.038	2.951TyrLeu: 2.951 ± 0.076	0.469TyrMet: 0.469 ± 0.026	0.786TyrAsn: 0.786 ± 0.038	1.401TyrPro: 1.401 ± 0.047	1.43TyrGln: 1.43 ± 0.053	1.939TyrArg: 1.939 ± 0.052	1.652TyrSer: 1.652 ± 0.051	1.32TyrThr: 1.32 ± 0.053	1.658TyrVal: 1.658 ± 0.05	0.418TyrTrp: 0.418 ± 0.024	0.758TyrTyr: 0.758 ± 0.039	0.006TyrXaa: 0.006 ± 0.003
Xaa	0.021XaaAla: 0.021 ± 0.006	0.002XaaCys: 0.002 ± 0.002	0.006XaaAsp: 0.006 ± 0.003	0.008XaaGlu: 0.008 ± 0.003	0.005XaaPhe: 0.005 ± 0.003	0.018XaaGly: 0.018 ± 0.005	0.005XaaHis: 0.005 ± 0.003	0.008XaaIle: 0.008 ± 0.003	0.006XaaLys: 0.006 ± 0.003	0.012XaaLeu: 0.012 ± 0.004	0.006XaaMet: 0.006 ± 0.003	0.006XaaAsn: 0.006 ± 0.003	0.021XaaPro: 0.021 ± 0.006	0.003XaaGln: 0.003 ± 0.002	0.018XaaArg: 0.018 ± 0.006	0.011XaaSer: 0.011 ± 0.004	0.008XaaThr: 0.008 ± 0.004	0.006XaaVal: 0.006 ± 0.003	0.002XaaTrp: 0.002 ± 0.001	0.005XaaTyr: 0.005 ± 0.003	1.72XaaXaa: 1.72 ± 0.191

Statistics based on 2003 proteins (657404 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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