Amino acid dipepetide frequency for Hymenobacter sp. CRA2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.979AlaAla: 14.979 ± 0.152
0.842AlaCys: 0.842 ± 0.026
6.019AlaAsp: 6.019 ± 0.064
6.336AlaGlu: 6.336 ± 0.089
3.704AlaPhe: 3.704 ± 0.053
9.204AlaGly: 9.204 ± 0.099
2.035AlaHis: 2.035 ± 0.038
3.634AlaIle: 3.634 ± 0.052
3.312AlaLys: 3.312 ± 0.058
11.379AlaLeu: 11.379 ± 0.116
1.83AlaMet: 1.83 ± 0.038
3.231AlaAsn: 3.231 ± 0.062
5.808AlaPro: 5.808 ± 0.083
5.652AlaGln: 5.652 ± 0.069
6.36AlaArg: 6.36 ± 0.074
5.454AlaSer: 5.454 ± 0.071
6.711AlaThr: 6.711 ± 0.096
7.698AlaVal: 7.698 ± 0.077
1.345AlaTrp: 1.345 ± 0.033
3.326AlaTyr: 3.326 ± 0.053
0.0AlaXaa: 0.0 ± 0.0
Cys
0.67CysAla: 0.67 ± 0.024
0.106CysCys: 0.106 ± 0.008
0.35CysAsp: 0.35 ± 0.015
0.333CysGlu: 0.333 ± 0.015
0.303CysPhe: 0.303 ± 0.014
0.648CysGly: 0.648 ± 0.023
0.19CysHis: 0.19 ± 0.013
0.317CysIle: 0.317 ± 0.014
0.165CysLys: 0.165 ± 0.009
0.785CysLeu: 0.785 ± 0.021
0.121CysMet: 0.121 ± 0.008
0.25CysAsn: 0.25 ± 0.013
0.44CysPro: 0.44 ± 0.021
0.362CysGln: 0.362 ± 0.014
0.494CysArg: 0.494 ± 0.017
0.42CysSer: 0.42 ± 0.017
0.457CysThr: 0.457 ± 0.019
0.474CysVal: 0.474 ± 0.017
0.118CysTrp: 0.118 ± 0.01
0.284CysTyr: 0.284 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
5.542AspAla: 5.542 ± 0.061
0.338AspCys: 0.338 ± 0.017
2.376AspAsp: 2.376 ± 0.044
3.155AspGlu: 3.155 ± 0.051
2.419AspPhe: 2.419 ± 0.042
3.925AspGly: 3.925 ± 0.072
0.892AspHis: 0.892 ± 0.026
2.245AspIle: 2.245 ± 0.042
2.137AspLys: 2.137 ± 0.044
4.845AspLeu: 4.845 ± 0.068
0.952AspMet: 0.952 ± 0.027
1.973AspAsn: 1.973 ± 0.037
2.379AspPro: 2.379 ± 0.042
2.052AspGln: 2.052 ± 0.035
2.54AspArg: 2.54 ± 0.044
2.46AspSer: 2.46 ± 0.044
2.512AspThr: 2.512 ± 0.046
3.86AspVal: 3.86 ± 0.054
0.833AspTrp: 0.833 ± 0.027
2.179AspTyr: 2.179 ± 0.046
0.0AspXaa: 0.0 ± 0.0
Glu
5.865GluAla: 5.865 ± 0.083
0.296GluCys: 0.296 ± 0.014
2.214GluAsp: 2.214 ± 0.047
3.037GluGlu: 3.037 ± 0.06
1.981GluPhe: 1.981 ± 0.034
3.261GluGly: 3.261 ± 0.05
1.283GluHis: 1.283 ± 0.033
2.185GluIle: 2.185 ± 0.039
2.222GluLys: 2.222 ± 0.051
6.675GluLeu: 6.675 ± 0.072
1.117GluMet: 1.117 ± 0.028
1.682GluAsn: 1.682 ± 0.033
2.372GluPro: 2.372 ± 0.041
3.507GluGln: 3.507 ± 0.057
3.482GluArg: 3.482 ± 0.051
2.136GluSer: 2.136 ± 0.035
2.536GluThr: 2.536 ± 0.04
3.86GluVal: 3.86 ± 0.053
0.69GluTrp: 0.69 ± 0.021
1.808GluTyr: 1.808 ± 0.035
0.0GluXaa: 0.0 ± 0.0
Phe
3.716PheAla: 3.716 ± 0.047
0.339PheCys: 0.339 ± 0.014
2.417PheAsp: 2.417 ± 0.041
2.141PheGlu: 2.141 ± 0.035
1.688PhePhe: 1.688 ± 0.034
3.412PheGly: 3.412 ± 0.05
0.737PheHis: 0.737 ± 0.023
1.571PheIle: 1.571 ± 0.037
1.251PheLys: 1.251 ± 0.028
3.524PheLeu: 3.524 ± 0.06
0.7PheMet: 0.7 ± 0.02
1.523PheAsn: 1.523 ± 0.033
1.675PhePro: 1.675 ± 0.033
1.494PheGln: 1.494 ± 0.033
2.647PheArg: 2.647 ± 0.045
2.414PheSer: 2.414 ± 0.04
2.582PheThr: 2.582 ± 0.05
2.899PheVal: 2.899 ± 0.041
0.552PheTrp: 0.552 ± 0.02
1.449PheTyr: 1.449 ± 0.032
0.0PheXaa: 0.0 ± 0.0
Gly
7.291GlyAla: 7.291 ± 0.076
0.67GlyCys: 0.67 ± 0.023
3.102GlyAsp: 3.102 ± 0.063
3.521GlyGlu: 3.521 ± 0.054
3.159GlyPhe: 3.159 ± 0.049
6.345GlyGly: 6.345 ± 0.112
1.644GlyHis: 1.644 ± 0.034
3.514GlyIle: 3.514 ± 0.054
3.218GlyLys: 3.218 ± 0.056
8.29GlyLeu: 8.29 ± 0.084
1.523GlyMet: 1.523 ± 0.037
2.811GlyAsn: 2.811 ± 0.058
3.151GlyPro: 3.151 ± 0.046
4.249GlyGln: 4.249 ± 0.05
5.245GlyArg: 5.245 ± 0.068
4.781GlySer: 4.781 ± 0.09
5.412GlyThr: 5.412 ± 0.11
5.257GlyVal: 5.257 ± 0.061
1.202GlyTrp: 1.202 ± 0.029
3.092GlyTyr: 3.092 ± 0.044
0.0GlyXaa: 0.0 ± 0.0
His
1.889HisAla: 1.889 ± 0.035
0.222HisCys: 0.222 ± 0.013
1.112HisAsp: 1.112 ± 0.029
1.16HisGlu: 1.16 ± 0.03
1.03HisPhe: 1.03 ± 0.024
1.611HisGly: 1.611 ± 0.035
0.639HisHis: 0.639 ± 0.025
0.879HisIle: 0.879 ± 0.024
0.618HisLys: 0.618 ± 0.022
2.483HisLeu: 2.483 ± 0.047
0.364HisMet: 0.364 ± 0.015
0.697HisAsn: 0.697 ± 0.019
1.309HisPro: 1.309 ± 0.031
0.97HisGln: 0.97 ± 0.028
1.388HisArg: 1.388 ± 0.03
1.007HisSer: 1.007 ± 0.027
1.17HisThr: 1.17 ± 0.029
1.371HisVal: 1.371 ± 0.034
0.343HisTrp: 0.343 ± 0.016
0.919HisTyr: 0.919 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
3.42IleAla: 3.42 ± 0.052
0.363IleCys: 0.363 ± 0.016
2.46IleAsp: 2.46 ± 0.045
2.434IleGlu: 2.434 ± 0.045
1.405IlePhe: 1.405 ± 0.033
3.616IleGly: 3.616 ± 0.052
0.776IleHis: 0.776 ± 0.025
2.069IleIle: 2.069 ± 0.05
1.628IleLys: 1.628 ± 0.035
3.34IleLeu: 3.34 ± 0.046
0.715IleMet: 0.715 ± 0.02
1.71IleAsn: 1.71 ± 0.038
1.944IlePro: 1.944 ± 0.033
1.536IleGln: 1.536 ± 0.035
2.742IleArg: 2.742 ± 0.043
2.63IleSer: 2.63 ± 0.038
2.645IleThr: 2.645 ± 0.049
2.769IleVal: 2.769 ± 0.047
0.481IleTrp: 0.481 ± 0.018
1.334IleTyr: 1.334 ± 0.029
0.0IleXaa: 0.0 ± 0.0
Lys
3.669LysAla: 3.669 ± 0.061
0.152LysCys: 0.152 ± 0.01
1.874LysAsp: 1.874 ± 0.041
1.948LysGlu: 1.948 ± 0.044
1.195LysPhe: 1.195 ± 0.026
2.511LysGly: 2.511 ± 0.045
0.776LysHis: 0.776 ± 0.025
1.508LysIle: 1.508 ± 0.036
1.819LysLys: 1.819 ± 0.047
3.778LysLeu: 3.778 ± 0.059
0.847LysMet: 0.847 ± 0.024
1.34LysAsn: 1.34 ± 0.031
2.093LysPro: 2.093 ± 0.043
1.797LysGln: 1.797 ± 0.036
2.016LysArg: 2.016 ± 0.039
1.716LysSer: 1.716 ± 0.034
2.096LysThr: 2.096 ± 0.037
2.605LysVal: 2.605 ± 0.044
0.384LysTrp: 0.384 ± 0.016
1.314LysTyr: 1.314 ± 0.032
0.0LysXaa: 0.0 ± 0.0
Leu
11.752LeuAla: 11.752 ± 0.112
0.816LeuCys: 0.816 ± 0.023
5.423LeuAsp: 5.423 ± 0.059
4.885LeuGlu: 4.885 ± 0.073
3.675LeuPhe: 3.675 ± 0.053
7.928LeuGly: 7.928 ± 0.074
2.459LeuHis: 2.459 ± 0.045
3.981LeuIle: 3.981 ± 0.057
3.661LeuLys: 3.661 ± 0.058
13.73LeuLeu: 13.73 ± 0.152
1.971LeuMet: 1.971 ± 0.036
4.073LeuAsn: 4.073 ± 0.061
6.757LeuPro: 6.757 ± 0.086
4.922LeuGln: 4.922 ± 0.055
8.339LeuArg: 8.339 ± 0.1
6.33LeuSer: 6.33 ± 0.075
7.409LeuThr: 7.409 ± 0.08
7.309LeuVal: 7.309 ± 0.088
1.251LeuTrp: 1.251 ± 0.03
3.164LeuTyr: 3.164 ± 0.046
0.0LeuXaa: 0.0 ± 0.0
Met
1.885MetAla: 1.885 ± 0.037
0.105MetCys: 0.105 ± 0.008
0.756MetAsp: 0.756 ± 0.024
0.882MetGlu: 0.882 ± 0.024
0.543MetPhe: 0.543 ± 0.021
1.29MetGly: 1.29 ± 0.033
0.435MetHis: 0.435 ± 0.016
0.674MetIle: 0.674 ± 0.022
0.945MetLys: 0.945 ± 0.025
2.123MetLeu: 2.123 ± 0.037
0.388MetMet: 0.388 ± 0.019
0.737MetAsn: 0.737 ± 0.023
1.224MetPro: 1.224 ± 0.027
0.989MetGln: 0.989 ± 0.027
1.283MetArg: 1.283 ± 0.028
1.099MetSer: 1.099 ± 0.029
1.01MetThr: 1.01 ± 0.023
1.145MetVal: 1.145 ± 0.028
0.18MetTrp: 0.18 ± 0.011
0.465MetTyr: 0.465 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
3.501AsnAla: 3.501 ± 0.057
0.292AsnCys: 0.292 ± 0.014
1.739AsnAsp: 1.739 ± 0.037
1.666AsnGlu: 1.666 ± 0.032
1.439AsnPhe: 1.439 ± 0.037
3.174AsnGly: 3.174 ± 0.065
0.648AsnHis: 0.648 ± 0.02
1.579AsnIle: 1.579 ± 0.032
1.252AsnLys: 1.252 ± 0.031
3.628AsnLeu: 3.628 ± 0.064
0.636AsnMet: 0.636 ± 0.022
1.546AsnAsn: 1.546 ± 0.042
2.369AsnPro: 2.369 ± 0.041
1.628AsnGln: 1.628 ± 0.035
2.151AsnArg: 2.151 ± 0.041
1.915AsnSer: 1.915 ± 0.041
2.241AsnThr: 2.241 ± 0.051
2.665AsnVal: 2.665 ± 0.05
0.539AsnTrp: 0.539 ± 0.022
1.451AsnTyr: 1.451 ± 0.033
0.0AsnXaa: 0.0 ± 0.0
Pro
7.793ProAla: 7.793 ± 0.111
0.26ProCys: 0.26 ± 0.013
3.322ProAsp: 3.322 ± 0.046
3.493ProGlu: 3.493 ± 0.051
1.826ProPhe: 1.826 ± 0.033
4.309ProGly: 4.309 ± 0.055
1.027ProHis: 1.027 ± 0.029
1.835ProIle: 1.835 ± 0.038
1.647ProLys: 1.647 ± 0.037
5.203ProLeu: 5.203 ± 0.068
0.855ProMet: 0.855 ± 0.022
1.985ProAsn: 1.985 ± 0.037
2.2ProPro: 2.2 ± 0.05
2.283ProGln: 2.283 ± 0.039
2.7ProArg: 2.7 ± 0.05
2.47ProSer: 2.47 ± 0.043
3.411ProThr: 3.411 ± 0.058
4.315ProVal: 4.315 ± 0.05
0.635ProTrp: 0.635 ± 0.02
1.674ProTyr: 1.674 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
5.522GlnAla: 5.522 ± 0.062
0.248GlnCys: 0.248 ± 0.012
2.065GlnAsp: 2.065 ± 0.035
2.482GlnGlu: 2.482 ± 0.045
1.71GlnPhe: 1.71 ± 0.031
3.156GlnGly: 3.156 ± 0.049
1.354GlnHis: 1.354 ± 0.029
1.706GlnIle: 1.706 ± 0.036
1.639GlnLys: 1.639 ± 0.036
6.321GlnLeu: 6.321 ± 0.077
0.928GlnMet: 0.928 ± 0.03
1.739GlnAsn: 1.739 ± 0.04
3.298GlnPro: 3.298 ± 0.047
3.783GlnGln: 3.783 ± 0.066
3.756GlnArg: 3.756 ± 0.052
1.992GlnSer: 1.992 ± 0.039
2.542GlnThr: 2.542 ± 0.041
3.622GlnVal: 3.622 ± 0.053
0.705GlnTrp: 0.705 ± 0.022
1.63GlnTyr: 1.63 ± 0.031
0.0GlnXaa: 0.0 ± 0.0
Arg
5.789ArgAla: 5.789 ± 0.068
0.432ArgCys: 0.432 ± 0.017
2.822ArgAsp: 2.822 ± 0.052
3.314ArgGlu: 3.314 ± 0.053
2.823ArgPhe: 2.823 ± 0.046
4.065ArgGly: 4.065 ± 0.066
1.646ArgHis: 1.646 ± 0.032
2.97ArgIle: 2.97 ± 0.046
2.192ArgLys: 2.192 ± 0.042
7.664ArgLeu: 7.664 ± 0.089
1.36ArgMet: 1.36 ± 0.028
2.272ArgAsn: 2.272 ± 0.048
3.507ArgPro: 3.507 ± 0.061
3.931ArgGln: 3.931 ± 0.061
5.09ArgArg: 5.09 ± 0.074
2.97ArgSer: 2.97 ± 0.047
3.734ArgThr: 3.734 ± 0.055
4.609ArgVal: 4.609 ± 0.057
1.104ArgTrp: 1.104 ± 0.024
2.742ArgTyr: 2.742 ± 0.044
0.0ArgXaa: 0.0 ± 0.0
Ser
5.679SerAla: 5.679 ± 0.072
0.457SerCys: 0.457 ± 0.019
2.312SerAsp: 2.312 ± 0.04
2.287SerGlu: 2.287 ± 0.04
2.548SerPhe: 2.548 ± 0.035
4.856SerGly: 4.856 ± 0.073
0.978SerHis: 0.978 ± 0.025
2.459SerIle: 2.459 ± 0.039
1.768SerLys: 1.768 ± 0.042
5.471SerLeu: 5.471 ± 0.066
0.981SerMet: 0.981 ± 0.024
1.904SerAsn: 1.904 ± 0.04
2.736SerPro: 2.736 ± 0.048
2.104SerGln: 2.104 ± 0.037
3.064SerArg: 3.064 ± 0.041
3.406SerSer: 3.406 ± 0.067
3.58SerThr: 3.58 ± 0.067
3.903SerVal: 3.903 ± 0.06
0.788SerTrp: 0.788 ± 0.024
2.169SerTyr: 2.169 ± 0.038
0.0SerXaa: 0.0 ± 0.0
Thr
7.239ThrAla: 7.239 ± 0.113
0.366ThrCys: 0.366 ± 0.016
3.343ThrAsp: 3.343 ± 0.051
3.024ThrGlu: 3.024 ± 0.042
2.353ThrPhe: 2.353 ± 0.045
5.42ThrGly: 5.42 ± 0.084
1.089ThrHis: 1.089 ± 0.026
2.488ThrIle: 2.488 ± 0.052
1.87ThrLys: 1.87 ± 0.04
6.502ThrLeu: 6.502 ± 0.079
0.87ThrMet: 0.87 ± 0.024
2.199ThrAsn: 2.199 ± 0.055
3.847ThrPro: 3.847 ± 0.056
2.351ThrGln: 2.351 ± 0.042
3.208ThrArg: 3.208 ± 0.054
3.432ThrSer: 3.432 ± 0.069
4.206ThrThr: 4.206 ± 0.095
4.913ThrVal: 4.913 ± 0.081
0.815ThrTrp: 0.815 ± 0.026
2.141ThrTyr: 2.141 ± 0.044
0.0ThrXaa: 0.0 ± 0.0
Val
7.97ValAla: 7.97 ± 0.082
0.545ValCys: 0.545 ± 0.018
3.36ValAsp: 3.36 ± 0.048
3.756ValGlu: 3.756 ± 0.056
2.718ValPhe: 2.718 ± 0.044
5.199ValGly: 5.199 ± 0.067
1.341ValHis: 1.341 ± 0.035
2.818ValIle: 2.818 ± 0.047
2.545ValLys: 2.545 ± 0.044
8.31ValLeu: 8.31 ± 0.078
1.26ValMet: 1.26 ± 0.028
2.442ValAsn: 2.442 ± 0.046
3.866ValPro: 3.866 ± 0.062
3.624ValGln: 3.624 ± 0.05
4.869ValArg: 4.869 ± 0.051
4.129ValSer: 4.129 ± 0.056
4.559ValThr: 4.559 ± 0.083
5.911ValVal: 5.911 ± 0.083
0.867ValTrp: 0.867 ± 0.026
2.349ValTyr: 2.349 ± 0.039
0.0ValXaa: 0.0 ± 0.0
Trp
1.3TrpAla: 1.3 ± 0.036
0.102TrpCys: 0.102 ± 0.008
0.585TrpAsp: 0.585 ± 0.023
0.617TrpGlu: 0.617 ± 0.024
0.532TrpPhe: 0.532 ± 0.019
0.866TrpGly: 0.866 ± 0.025
0.375TrpHis: 0.375 ± 0.016
0.411TrpIle: 0.411 ± 0.015
0.431TrpLys: 0.431 ± 0.018
2.014TrpLeu: 2.014 ± 0.046
0.256TrpMet: 0.256 ± 0.013
0.51TrpAsn: 0.51 ± 0.019
0.603TrpPro: 0.603 ± 0.019
0.998TrpGln: 0.998 ± 0.028
1.005TrpArg: 1.005 ± 0.028
0.666TrpSer: 0.666 ± 0.023
0.733TrpThr: 0.733 ± 0.021
0.903TrpVal: 0.903 ± 0.026
0.247TrpTrp: 0.247 ± 0.012
0.458TrpTyr: 0.458 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.387TyrAla: 3.387 ± 0.046
0.334TyrCys: 0.334 ± 0.015
1.966TyrAsp: 1.966 ± 0.039
1.734TyrGlu: 1.734 ± 0.034
1.681TyrPhe: 1.681 ± 0.033
2.683TyrGly: 2.683 ± 0.048
0.855TyrHis: 0.855 ± 0.024
1.173TyrIle: 1.173 ± 0.029
1.119TyrLys: 1.119 ± 0.027
3.77TyrLeu: 3.77 ± 0.045
0.482TyrMet: 0.482 ± 0.02
1.436TyrAsn: 1.436 ± 0.034
1.664TyrPro: 1.664 ± 0.041
1.967TyrGln: 1.967 ± 0.041
2.661TyrArg: 2.661 ± 0.041
2.022TyrSer: 2.022 ± 0.036
2.129TyrThr: 2.129 ± 0.039
2.352TyrVal: 2.352 ± 0.035
0.534TyrTrp: 0.534 ± 0.019
1.534TyrTyr: 1.534 ± 0.038
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4792 proteins (1648900 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski