Desulfocurvibacter africanus subsp. africanus str. Walvis Bay

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfovibrionales; Desulfovibrionaceae; Desulfocurvibacter; Desulfocurvibacter africanus; Desulfocurvibacter africanus subsp. africanus

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3699 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F3Z1C3|F3Z1C3_DESAF Uncharacterized protein OS=Desulfocurvibacter africanus subsp. africanus str. Walvis Bay OX=690850 GN=Desaf_2813 PE=4 SV=1
MM1 pKa = 7.78SDD3 pKa = 3.05AKK5 pKa = 10.79SAIGTVVKK13 pKa = 10.74ISGQVFAEE21 pKa = 4.34ADD23 pKa = 3.64GHH25 pKa = 5.09RR26 pKa = 11.84HH27 pKa = 5.53PLAEE31 pKa = 4.33GSTVRR36 pKa = 11.84EE37 pKa = 4.34DD38 pKa = 3.66EE39 pKa = 4.55TVVTADD45 pKa = 3.26GSKK48 pKa = 10.32VEE50 pKa = 5.59IRR52 pKa = 11.84FTDD55 pKa = 3.49NTVLSQGEE63 pKa = 3.95NSRR66 pKa = 11.84IEE68 pKa = 3.92LDD70 pKa = 3.44EE71 pKa = 3.89YY72 pKa = 11.43VYY74 pKa = 11.1EE75 pKa = 4.44GDD77 pKa = 3.83KK78 pKa = 11.18GAAGLLFNMVQGSFRR93 pKa = 11.84TVTGKK98 pKa = 9.85IVEE101 pKa = 4.18QNPEE105 pKa = 4.07GFNLSSPLATIGIRR119 pKa = 11.84GTTTFHH125 pKa = 6.99QIGPDD130 pKa = 3.85GEE132 pKa = 4.22RR133 pKa = 11.84HH134 pKa = 4.88GVQNIANPSLGGLDD148 pKa = 3.82SPLHH152 pKa = 5.57GMPSGYY158 pKa = 8.48HH159 pKa = 4.76TMLVTFSNGEE169 pKa = 3.89TRR171 pKa = 11.84VIADD175 pKa = 4.2SYY177 pKa = 11.67QMVDD181 pKa = 3.46LTSAGIGMIRR191 pKa = 11.84TFSLNEE197 pKa = 3.78LLDD200 pKa = 4.87FIDD203 pKa = 5.33LSAHH207 pKa = 5.28SPEE210 pKa = 4.15EE211 pKa = 4.09RR212 pKa = 11.84EE213 pKa = 4.32SLVDD217 pKa = 3.51TLLHH221 pKa = 6.16EE222 pKa = 5.0FRR224 pKa = 11.84EE225 pKa = 4.39QVPPEE230 pKa = 4.25PQSEE234 pKa = 4.42HH235 pKa = 5.94QSSSGDD241 pKa = 3.44NLSPDD246 pKa = 3.35VTDD249 pKa = 6.37DD250 pKa = 3.47GTQDD254 pKa = 3.91SDD256 pKa = 5.74GEE258 pKa = 4.14DD259 pKa = 3.09QQAQDD264 pKa = 4.45EE265 pKa = 4.8VPPDD269 pKa = 3.39IQRR272 pKa = 11.84IISDD276 pKa = 4.32PLPEE280 pKa = 4.14PVIQGGGGNGGGWNPPQEE298 pKa = 4.77DD299 pKa = 4.11PPVRR303 pKa = 11.84DD304 pKa = 3.67VIEE307 pKa = 4.63IIPRR311 pKa = 11.84ITFPIFLIGDD321 pKa = 4.28GGNNSIEE328 pKa = 4.06GTEE331 pKa = 4.13YY332 pKa = 10.39PDD334 pKa = 5.61FIQGLAGDD342 pKa = 4.18DD343 pKa = 4.09SLWGLGSNDD352 pKa = 3.38TLQGGEE358 pKa = 4.49GNDD361 pKa = 3.53VLYY364 pKa = 11.01GGAGDD369 pKa = 4.75DD370 pKa = 3.77VLDD373 pKa = 4.5GGRR376 pKa = 11.84GDD378 pKa = 3.68NSIDD382 pKa = 3.6GGAGNNWVSYY392 pKa = 10.98ASVRR396 pKa = 11.84DD397 pKa = 3.6TFQNKK402 pKa = 8.8GVVVYY407 pKa = 10.81LDD409 pKa = 4.61DD410 pKa = 5.39EE411 pKa = 6.25GDD413 pKa = 3.31DD414 pKa = 3.89SYY416 pKa = 12.02GVRR419 pKa = 11.84EE420 pKa = 4.08YY421 pKa = 10.35PVYY424 pKa = 10.44EE425 pKa = 4.29SNGNLLSEE433 pKa = 4.29PHH435 pKa = 6.3SEE437 pKa = 4.05YY438 pKa = 11.2DD439 pKa = 3.91DD440 pKa = 4.2LASIANVEE448 pKa = 4.05GSIFSDD454 pKa = 4.13AIYY457 pKa = 10.83GNDD460 pKa = 4.01HH461 pKa = 6.43NNHH464 pKa = 6.17LKK466 pKa = 10.72GLAGDD471 pKa = 4.39DD472 pKa = 4.32SIIGNAGDD480 pKa = 3.73DD481 pKa = 4.12TLDD484 pKa = 3.79GGQGNDD490 pKa = 4.06TIDD493 pKa = 3.64GGLGSDD499 pKa = 4.12VIIGGSGNDD508 pKa = 3.06WVSYY512 pKa = 9.35QSYY515 pKa = 9.69YY516 pKa = 10.63SCSFQGMSIDD526 pKa = 4.38LVNGACSLDD535 pKa = 3.63TSSEE539 pKa = 4.06DD540 pKa = 3.25TDD542 pKa = 3.45QFSGIEE548 pKa = 3.95NVEE551 pKa = 3.72GSEE554 pKa = 4.27YY555 pKa = 10.92GDD557 pKa = 4.36EE558 pKa = 4.19ICGDD562 pKa = 3.65SLGNTLHH569 pKa = 6.79GMGGSDD575 pKa = 4.27TISGGAGSDD584 pKa = 3.95FINGGEE590 pKa = 4.97GIDD593 pKa = 3.6TLYY596 pKa = 11.46GNAGADD602 pKa = 3.08TFYY605 pKa = 10.75FDD607 pKa = 4.37NMEE610 pKa = 5.12DD611 pKa = 3.63GFSVDD616 pKa = 2.93THH618 pKa = 8.34ISFSGQQGDD627 pKa = 4.3LIKK630 pKa = 10.95DD631 pKa = 3.79FTPGQDD637 pKa = 4.05KK638 pKa = 10.81ILLDD642 pKa = 3.71SSQYY646 pKa = 9.65SQLGMVVSYY655 pKa = 7.96DD656 pKa = 3.62TNFITIDD663 pKa = 3.37GLYY666 pKa = 10.45DD667 pKa = 3.47GNNATDD673 pKa = 3.37VQGAYY678 pKa = 10.46SSWLNGEE685 pKa = 3.68ACLIVDD691 pKa = 3.76RR692 pKa = 11.84DD693 pKa = 4.01YY694 pKa = 12.18VNDD697 pKa = 4.04CCRR700 pKa = 11.84LIYY703 pKa = 10.38DD704 pKa = 4.59EE705 pKa = 5.75NGDD708 pKa = 3.75DD709 pKa = 3.38PGYY712 pKa = 10.74YY713 pKa = 10.22VLATIEE719 pKa = 4.4GSDD722 pKa = 4.24TVLTDD727 pKa = 2.96QDD729 pKa = 4.19VVVTPMAA736 pKa = 4.53

Molecular weight: 78.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F3YZS7|F3YZS7_DESAF Putative PAS/PAC sensor protein OS=Desulfocurvibacter africanus subsp. africanus str. Walvis Bay OX=690850 GN=Desaf_2561 PE=4 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.95RR4 pKa = 11.84TYY6 pKa = 10.09QPSKK10 pKa = 9.44IKK12 pKa = 10.44RR13 pKa = 11.84KK14 pKa = 7.56RR15 pKa = 11.84THH17 pKa = 5.78GFRR20 pKa = 11.84VRR22 pKa = 11.84MSTKK26 pKa = 10.1NGRR29 pKa = 11.84NMLNRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.05GRR40 pKa = 11.84QRR42 pKa = 11.84LSAA45 pKa = 4.04

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3699

0

3699

1178996

30

1935

318.7

35.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.552 ± 0.05

1.253 ± 0.018

5.273 ± 0.026

6.573 ± 0.035

3.808 ± 0.025

8.201 ± 0.032

2.037 ± 0.019

4.858 ± 0.033

4.044 ± 0.038

11.278 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.713 ± 0.021

2.633 ± 0.019

4.897 ± 0.029

3.536 ± 0.027

7.019 ± 0.034

5.667 ± 0.024

4.792 ± 0.026

7.147 ± 0.029

1.245 ± 0.017

2.474 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski