Amino acid dipepetide frequency for Flectobacillus sp. BAB-3569

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.387AlaAla: 4.387 ± 0.077	0.675AlaCys: 0.675 ± 0.02	3.518AlaAsp: 3.518 ± 0.051	4.251AlaGlu: 4.251 ± 0.064	3.347AlaPhe: 3.347 ± 0.045	4.745AlaGly: 4.745 ± 0.069	1.124AlaHis: 1.124 ± 0.031	5.081AlaIle: 5.081 ± 0.063	4.905AlaLys: 4.905 ± 0.073	6.709AlaLeu: 6.709 ± 0.077	1.572AlaMet: 1.572 ± 0.034	3.849AlaAsn: 3.849 ± 0.052	2.367AlaPro: 2.367 ± 0.044	3.408AlaGln: 3.408 ± 0.049	2.231AlaArg: 2.231 ± 0.032	4.965AlaSer: 4.965 ± 0.067	4.213AlaThr: 4.213 ± 0.075	4.253AlaVal: 4.253 ± 0.059	0.863AlaTrp: 0.863 ± 0.025	2.717AlaTyr: 2.717 ± 0.046	0.001AlaXaa: 0.001 ± 0.001
Cys	0.479CysAla: 0.479 ± 0.017	0.113CysCys: 0.113 ± 0.009	0.434CysAsp: 0.434 ± 0.017	0.502CysGlu: 0.502 ± 0.022	0.447CysPhe: 0.447 ± 0.014	0.613CysGly: 0.613 ± 0.021	0.218CysHis: 0.218 ± 0.012	0.587CysIle: 0.587 ± 0.02	0.507CysLys: 0.507 ± 0.021	0.811CysLeu: 0.811 ± 0.024	0.185CysMet: 0.185 ± 0.011	0.4CysAsn: 0.4 ± 0.016	0.362CysPro: 0.362 ± 0.015	0.363CysGln: 0.363 ± 0.014	0.289CysArg: 0.289 ± 0.013	0.627CysSer: 0.627 ± 0.022	0.483CysThr: 0.483 ± 0.019	0.519CysVal: 0.519 ± 0.017	0.1CysTrp: 0.1 ± 0.008	0.338CysTyr: 0.338 ± 0.014	0.001CysXaa: 0.001 ± 0.001
Asp	3.687AspAla: 3.687 ± 0.048	0.427AspCys: 0.427 ± 0.017	2.259AspAsp: 2.259 ± 0.039	3.243AspGlu: 3.243 ± 0.057	3.269AspPhe: 3.269 ± 0.049	3.763AspGly: 3.763 ± 0.073	0.738AspHis: 0.738 ± 0.021	3.745AspIle: 3.745 ± 0.049	3.724AspLys: 3.724 ± 0.053	5.028AspLeu: 5.028 ± 0.063	1.047AspMet: 1.047 ± 0.02	2.719AspAsn: 2.719 ± 0.046	1.756AspPro: 1.756 ± 0.038	1.429AspGln: 1.429 ± 0.027	1.759AspArg: 1.759 ± 0.031	3.105AspSer: 3.105 ± 0.043	2.761AspThr: 2.761 ± 0.046	3.05AspVal: 3.05 ± 0.044	0.854AspTrp: 0.854 ± 0.025	2.455AspTyr: 2.455 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	4.368GluAla: 4.368 ± 0.066	0.35GluCys: 0.35 ± 0.016	2.809GluAsp: 2.809 ± 0.04	4.116GluGlu: 4.116 ± 0.086	2.519GluPhe: 2.519 ± 0.039	3.623GluGly: 3.623 ± 0.047	1.088GluHis: 1.088 ± 0.028	4.746GluIle: 4.746 ± 0.059	5.425GluLys: 5.425 ± 0.083	5.833GluLeu: 5.833 ± 0.065	1.433GluMet: 1.433 ± 0.03	3.654GluAsn: 3.654 ± 0.051	1.681GluPro: 1.681 ± 0.035	2.621GluGln: 2.621 ± 0.052	2.497GluArg: 2.497 ± 0.04	3.377GluSer: 3.377 ± 0.048	2.985GluThr: 2.985 ± 0.041	4.167GluVal: 4.167 ± 0.055	0.706GluTrp: 0.706 ± 0.021	2.352GluTyr: 2.352 ± 0.042	0.001GluXaa: 0.001 ± 0.001
Phe	3.192PheAla: 3.192 ± 0.041	0.538PheCys: 0.538 ± 0.018	2.976PheAsp: 2.976 ± 0.039	3.349PheGlu: 3.349 ± 0.049	2.428PhePhe: 2.428 ± 0.047	3.478PheGly: 3.478 ± 0.05	0.841PheHis: 0.841 ± 0.025	3.081PheIle: 3.081 ± 0.054	3.149PheLys: 3.149 ± 0.044	4.553PheLeu: 4.553 ± 0.059	1.129PheMet: 1.129 ± 0.028	2.644PheAsn: 2.644 ± 0.043	1.714PhePro: 1.714 ± 0.033	1.866PheGln: 1.866 ± 0.036	1.883PheArg: 1.883 ± 0.033	3.862PheSer: 3.862 ± 0.056	2.921PheThr: 2.921 ± 0.042	3.23PheVal: 3.23 ± 0.044	0.732PheTrp: 0.732 ± 0.024	2.055PheTyr: 2.055 ± 0.039	0.002PheXaa: 0.002 ± 0.001
Gly	4.331GlyAla: 4.331 ± 0.068	0.651GlyCys: 0.651 ± 0.024	3.114GlyAsp: 3.114 ± 0.05	3.248GlyGlu: 3.248 ± 0.047	3.636GlyPhe: 3.636 ± 0.056	4.717GlyGly: 4.717 ± 0.083	1.115GlyHis: 1.115 ± 0.026	5.333GlyIle: 5.333 ± 0.057	5.394GlyLys: 5.394 ± 0.064	6.264GlyLeu: 6.264 ± 0.08	1.599GlyMet: 1.599 ± 0.033	3.579GlyAsn: 3.579 ± 0.064	1.29GlyPro: 1.29 ± 0.032	2.435GlyGln: 2.435 ± 0.042	2.383GlyArg: 2.383 ± 0.036	4.159GlySer: 4.159 ± 0.068	4.237GlyThr: 4.237 ± 0.068	4.67GlyVal: 4.67 ± 0.055	0.935GlyTrp: 0.935 ± 0.023	3.015GlyTyr: 3.015 ± 0.049	0.0GlyXaa: 0.0 ± 0.0
His	1.064HisAla: 1.064 ± 0.026	0.181HisCys: 0.181 ± 0.01	0.802HisAsp: 0.802 ± 0.022	1.081HisGlu: 1.081 ± 0.03	1.154HisPhe: 1.154 ± 0.026	1.068HisGly: 1.068 ± 0.026	0.525HisHis: 0.525 ± 0.021	1.363HisIle: 1.363 ± 0.032	1.071HisLys: 1.071 ± 0.027	1.838HisLeu: 1.838 ± 0.037	0.211HisMet: 0.211 ± 0.01	0.854HisAsn: 0.854 ± 0.021	0.964HisPro: 0.964 ± 0.023	0.874HisGln: 0.874 ± 0.025	0.785HisArg: 0.785 ± 0.021	1.035HisSer: 1.035 ± 0.024	0.999HisThr: 0.999 ± 0.026	0.899HisVal: 0.899 ± 0.026	0.324HisTrp: 0.324 ± 0.015	0.814HisTyr: 0.814 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	5.347IleAla: 5.347 ± 0.055	0.731IleCys: 0.731 ± 0.022	4.054IleAsp: 4.054 ± 0.052	4.655IleGlu: 4.655 ± 0.068	3.176IlePhe: 3.176 ± 0.056	4.744IleGly: 4.744 ± 0.055	1.316IleHis: 1.316 ± 0.031	4.599IleIle: 4.599 ± 0.064	4.776IleLys: 4.776 ± 0.06	6.638IleLeu: 6.638 ± 0.078	1.288IleMet: 1.288 ± 0.03	3.846IleAsn: 3.846 ± 0.051	3.191IlePro: 3.191 ± 0.04	3.064IleGln: 3.064 ± 0.046	2.795IleArg: 2.795 ± 0.041	5.252IleSer: 5.252 ± 0.061	4.217IleThr: 4.217 ± 0.054	4.329IleVal: 4.329 ± 0.058	0.786IleTrp: 0.786 ± 0.021	2.563IleTyr: 2.563 ± 0.038	0.001IleXaa: 0.001 ± 0.001
Lys	5.371LysAla: 5.371 ± 0.072	0.372LysCys: 0.372 ± 0.015	4.052LysAsp: 4.052 ± 0.056	4.756LysGlu: 4.756 ± 0.073	2.685LysPhe: 2.685 ± 0.045	4.682LysGly: 4.682 ± 0.055	1.292LysHis: 1.292 ± 0.031	5.353LysIle: 5.353 ± 0.058	5.448LysLys: 5.448 ± 0.067	6.208LysLeu: 6.208 ± 0.069	1.923LysMet: 1.923 ± 0.03	4.317LysAsn: 4.317 ± 0.062	2.744LysPro: 2.744 ± 0.044	2.735LysGln: 2.735 ± 0.042	2.654LysArg: 2.654 ± 0.042	4.456LysSer: 4.456 ± 0.058	4.098LysThr: 4.098 ± 0.048	5.105LysVal: 5.105 ± 0.064	0.81LysTrp: 0.81 ± 0.023	2.996LysTyr: 2.996 ± 0.042	0.001LysXaa: 0.001 ± 0.001
Leu	6.682LeuAla: 6.682 ± 0.077	0.843LeuCys: 0.843 ± 0.021	4.842LeuAsp: 4.842 ± 0.051	5.689LeuGlu: 5.689 ± 0.062	4.506LeuPhe: 4.506 ± 0.057	6.389LeuGly: 6.389 ± 0.07	1.654LeuHis: 1.654 ± 0.032	6.175LeuIle: 6.175 ± 0.078	7.142LeuLys: 7.142 ± 0.078	9.285LeuLeu: 9.285 ± 0.096	2.213LeuMet: 2.213 ± 0.043	5.08LeuAsn: 5.08 ± 0.058	4.068LeuPro: 4.068 ± 0.051	3.826LeuGln: 3.826 ± 0.052	3.599LeuArg: 3.599 ± 0.05	7.363LeuSer: 7.363 ± 0.075	5.592LeuThr: 5.592 ± 0.066	5.832LeuVal: 5.832 ± 0.059	1.081LeuTrp: 1.081 ± 0.028	3.331LeuTyr: 3.331 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	1.669MetAla: 1.669 ± 0.031	0.141MetCys: 0.141 ± 0.01	1.01MetAsp: 1.01 ± 0.024	1.138MetGlu: 1.138 ± 0.026	0.829MetPhe: 0.829 ± 0.022	1.525MetGly: 1.525 ± 0.029	0.333MetHis: 0.333 ± 0.013	1.488MetIle: 1.488 ± 0.035	2.03MetLys: 2.03 ± 0.034	2.139MetLeu: 2.139 ± 0.034	0.634MetMet: 0.634 ± 0.019	1.271MetAsn: 1.271 ± 0.027	1.005MetPro: 1.005 ± 0.022	0.855MetGln: 0.855 ± 0.023	0.876MetArg: 0.876 ± 0.022	1.497MetSer: 1.497 ± 0.031	1.309MetThr: 1.309 ± 0.028	1.35MetVal: 1.35 ± 0.029	0.196MetTrp: 0.196 ± 0.011	0.709MetTyr: 0.709 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	3.909AsnAla: 3.909 ± 0.055	0.413AsnCys: 0.413 ± 0.017	2.645AsnAsp: 2.645 ± 0.047	2.88AsnGlu: 2.88 ± 0.047	2.792AsnPhe: 2.792 ± 0.049	3.813AsnGly: 3.813 ± 0.058	1.114AsnHis: 1.114 ± 0.026	4.044AsnIle: 4.044 ± 0.054	3.315AsnLys: 3.315 ± 0.049	5.329AsnLeu: 5.329 ± 0.064	1.1AsnMet: 1.1 ± 0.022	3.139AsnAsn: 3.139 ± 0.057	3.059AsnPro: 3.059 ± 0.042	2.624AsnGln: 2.624 ± 0.046	2.163AsnArg: 2.163 ± 0.037	3.464AsnSer: 3.464 ± 0.046	3.72AsnThr: 3.72 ± 0.054	3.134AsnVal: 3.134 ± 0.048	0.78AsnTrp: 0.78 ± 0.024	2.581AsnTyr: 2.581 ± 0.045	0.001AsnXaa: 0.001 ± 0.001
Pro	2.706ProAla: 2.706 ± 0.053	0.226ProCys: 0.226 ± 0.012	2.151ProAsp: 2.151 ± 0.037	2.768ProGlu: 2.768 ± 0.052	1.884ProPhe: 1.884 ± 0.035	1.498ProGly: 1.498 ± 0.033	0.702ProHis: 0.702 ± 0.023	2.805ProIle: 2.805 ± 0.039	2.587ProLys: 2.587 ± 0.041	3.271ProLeu: 3.271 ± 0.045	0.815ProMet: 0.815 ± 0.021	2.508ProAsn: 2.508 ± 0.042	0.949ProPro: 0.949 ± 0.028	1.591ProGln: 1.591 ± 0.031	1.122ProArg: 1.122 ± 0.027	2.878ProSer: 2.878 ± 0.052	2.436ProThr: 2.436 ± 0.046	2.67ProVal: 2.67 ± 0.041	0.428ProTrp: 0.428 ± 0.016	1.513ProTyr: 1.513 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	2.929GlnAla: 2.929 ± 0.047	0.226GlnCys: 0.226 ± 0.013	1.802GlnAsp: 1.802 ± 0.032	2.612GlnGlu: 2.612 ± 0.048	1.827GlnPhe: 1.827 ± 0.032	2.24GlnGly: 2.24 ± 0.043	0.753GlnHis: 0.753 ± 0.02	2.991GlnIle: 2.991 ± 0.038	3.615GlnLys: 3.615 ± 0.048	3.936GlnLeu: 3.936 ± 0.054	0.985GlnMet: 0.985 ± 0.022	2.634GlnAsn: 2.634 ± 0.041	1.384GlnPro: 1.384 ± 0.026	2.036GlnGln: 2.036 ± 0.04	1.515GlnArg: 1.515 ± 0.031	2.568GlnSer: 2.568 ± 0.034	2.507GlnThr: 2.507 ± 0.048	2.684GlnVal: 2.684 ± 0.044	0.527GlnTrp: 0.527 ± 0.02	1.699GlnTyr: 1.699 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	2.287ArgAla: 2.287 ± 0.041	0.252ArgCys: 0.252 ± 0.014	1.745ArgAsp: 1.745 ± 0.033	2.173ArgGlu: 2.173 ± 0.043	2.056ArgPhe: 2.056 ± 0.037	2.17ArgGly: 2.17 ± 0.037	0.653ArgHis: 0.653 ± 0.02	2.845ArgIle: 2.845 ± 0.041	2.782ArgLys: 2.782 ± 0.051	3.669ArgLeu: 3.669 ± 0.054	0.95ArgMet: 0.95 ± 0.024	2.136ArgAsn: 2.136 ± 0.039	1.332ArgPro: 1.332 ± 0.031	1.524ArgGln: 1.524 ± 0.031	1.498ArgArg: 1.498 ± 0.031	1.964ArgSer: 1.964 ± 0.035	2.054ArgThr: 2.054 ± 0.034	2.486ArgVal: 2.486 ± 0.041	0.544ArgTrp: 0.544 ± 0.019	1.662ArgTyr: 1.662 ± 0.031	0.001ArgXaa: 0.001 ± 0.001
Ser	4.456SerAla: 4.456 ± 0.069	0.617SerCys: 0.617 ± 0.02	3.325SerAsp: 3.325 ± 0.051	3.732SerGlu: 3.732 ± 0.049	3.736SerPhe: 3.736 ± 0.052	4.685SerGly: 4.685 ± 0.065	1.163SerHis: 1.163 ± 0.026	5.032SerIle: 5.032 ± 0.059	4.484SerLys: 4.484 ± 0.056	6.587SerLeu: 6.587 ± 0.071	1.35SerMet: 1.35 ± 0.029	3.73SerAsn: 3.73 ± 0.052	2.563SerPro: 2.563 ± 0.036	2.731SerGln: 2.731 ± 0.043	2.207SerArg: 2.207 ± 0.038	4.739SerSer: 4.739 ± 0.069	4.263SerThr: 4.263 ± 0.059	4.415SerVal: 4.415 ± 0.058	0.854SerTrp: 0.854 ± 0.024	2.879SerTyr: 2.879 ± 0.049	0.001SerXaa: 0.001 ± 0.001
Thr	4.153ThrAla: 4.153 ± 0.072	0.454ThrCys: 0.454 ± 0.024	3.045ThrAsp: 3.045 ± 0.047	3.169ThrGlu: 3.169 ± 0.046	3.066ThrPhe: 3.066 ± 0.044	4.444ThrGly: 4.444 ± 0.08	1.068ThrHis: 1.068 ± 0.032	4.378ThrIle: 4.378 ± 0.058	3.725ThrLys: 3.725 ± 0.049	5.747ThrLeu: 5.747 ± 0.062	1.025ThrMet: 1.025 ± 0.025	3.164ThrAsn: 3.164 ± 0.054	2.761ThrPro: 2.761 ± 0.04	2.425ThrGln: 2.425 ± 0.037	1.887ThrArg: 1.887 ± 0.031	4.006ThrSer: 4.006 ± 0.073	3.899ThrThr: 3.899 ± 0.091	3.885ThrVal: 3.885 ± 0.064	0.743ThrTrp: 0.743 ± 0.022	2.409ThrTyr: 2.409 ± 0.042	0.001ThrXaa: 0.001 ± 0.001
Val	4.621ValAla: 4.621 ± 0.052	0.647ValCys: 0.647 ± 0.023	3.386ValAsp: 3.386 ± 0.047	3.943ValGlu: 3.943 ± 0.053	3.183ValPhe: 3.183 ± 0.041	4.363ValGly: 4.363 ± 0.058	1.051ValHis: 1.051 ± 0.029	4.429ValIle: 4.429 ± 0.054	4.581ValLys: 4.581 ± 0.052	6.019ValLeu: 6.019 ± 0.065	1.399ValMet: 1.399 ± 0.032	3.511ValAsn: 3.511 ± 0.057	2.356ValPro: 2.356 ± 0.039	2.246ValGln: 2.246 ± 0.034	2.348ValArg: 2.348 ± 0.041	4.711ValSer: 4.711 ± 0.061	3.715ValThr: 3.715 ± 0.059	4.587ValVal: 4.587 ± 0.056	0.823ValTrp: 0.823 ± 0.023	2.428ValTyr: 2.428 ± 0.039	0.002ValXaa: 0.002 ± 0.001
Trp	0.838TrpAla: 0.838 ± 0.023	0.117TrpCys: 0.117 ± 0.008	0.653TrpAsp: 0.653 ± 0.019	0.773TrpGlu: 0.773 ± 0.023	0.621TrpPhe: 0.621 ± 0.018	0.935TrpGly: 0.935 ± 0.024	0.277TrpHis: 0.277 ± 0.013	0.875TrpIle: 0.875 ± 0.024	0.899TrpLys: 0.899 ± 0.025	1.244TrpLeu: 1.244 ± 0.026	0.368TrpMet: 0.368 ± 0.015	0.768TrpAsn: 0.768 ± 0.026	0.266TrpPro: 0.266 ± 0.014	0.655TrpGln: 0.655 ± 0.021	0.525TrpArg: 0.525 ± 0.017	0.846TrpSer: 0.846 ± 0.025	0.688TrpThr: 0.688 ± 0.022	0.825TrpVal: 0.825 ± 0.023	0.218TrpTrp: 0.218 ± 0.013	0.544TrpTyr: 0.544 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.703TyrAla: 2.703 ± 0.047	0.401TyrCys: 0.401 ± 0.015	2.195TyrAsp: 2.195 ± 0.037	2.274TyrGlu: 2.274 ± 0.038	2.461TyrPhe: 2.461 ± 0.042	2.61TyrGly: 2.61 ± 0.041	0.882TyrHis: 0.882 ± 0.023	2.419TyrIle: 2.419 ± 0.036	2.494TyrLys: 2.494 ± 0.043	4.081TyrLeu: 4.081 ± 0.056	0.778TyrMet: 0.778 ± 0.022	2.215TyrAsn: 2.215 ± 0.046	1.696TyrPro: 1.696 ± 0.031	2.161TyrGln: 2.161 ± 0.038	1.768TyrArg: 1.768 ± 0.03	2.673TyrSer: 2.673 ± 0.036	2.359TyrThr: 2.359 ± 0.04	2.261TyrVal: 2.261 ± 0.042	0.629TyrTrp: 0.629 ± 0.021	1.868TyrTyr: 1.868 ± 0.04	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.002XaaSer: 0.002 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.005XaaXaa: 0.005 ± 0.002

Statistics based on 5084 proteins (1755997 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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