Amino acid dipepetide frequency for Salmonella phage Skate

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.886AlaAla: 9.886 ± 2.591
0.597AlaCys: 0.597 ± 0.213
4.313AlaAsp: 4.313 ± 0.607
6.768AlaGlu: 6.768 ± 0.944
2.057AlaPhe: 2.057 ± 0.4
7.166AlaGly: 7.166 ± 0.647
0.863AlaHis: 0.863 ± 0.273
5.573AlaIle: 5.573 ± 0.63
5.971AlaLys: 5.971 ± 0.673
7.033AlaLeu: 7.033 ± 0.782
2.521AlaMet: 2.521 ± 0.532
3.384AlaAsn: 3.384 ± 0.514
1.858AlaPro: 1.858 ± 0.306
3.185AlaGln: 3.185 ± 1.078
5.042AlaArg: 5.042 ± 0.576
5.308AlaSer: 5.308 ± 0.725
4.777AlaThr: 4.777 ± 0.914
5.042AlaVal: 5.042 ± 0.692
1.261AlaTrp: 1.261 ± 0.265
2.322AlaTyr: 2.322 ± 0.433
0.0AlaXaa: 0.0 ± 0.0
Cys
1.128CysAla: 1.128 ± 0.266
0.398CysCys: 0.398 ± 0.163
1.062CysAsp: 1.062 ± 0.27
1.261CysGlu: 1.261 ± 0.294
0.73CysPhe: 0.73 ± 0.227
1.659CysGly: 1.659 ± 0.446
0.863CysHis: 0.863 ± 0.196
0.663CysIle: 0.663 ± 0.218
1.725CysLys: 1.725 ± 0.503
0.995CysLeu: 0.995 ± 0.259
0.199CysMet: 0.199 ± 0.106
0.73CysAsn: 0.73 ± 0.225
0.464CysPro: 0.464 ± 0.19
0.133CysGln: 0.133 ± 0.085
0.929CysArg: 0.929 ± 0.288
1.327CysSer: 1.327 ± 0.32
0.464CysThr: 0.464 ± 0.192
0.464CysVal: 0.464 ± 0.165
0.398CysTrp: 0.398 ± 0.164
0.332CysTyr: 0.332 ± 0.181
0.0CysXaa: 0.0 ± 0.0
Asp
5.242AspAla: 5.242 ± 0.723
1.062AspCys: 1.062 ± 0.278
4.578AspAsp: 4.578 ± 0.666
4.18AspGlu: 4.18 ± 0.526
2.787AspPhe: 2.787 ± 0.47
6.104AspGly: 6.104 ± 0.66
0.995AspHis: 0.995 ± 0.231
4.578AspIle: 4.578 ± 0.536
3.516AspLys: 3.516 ± 0.549
3.118AspLeu: 3.118 ± 0.505
2.123AspMet: 2.123 ± 0.373
2.389AspAsn: 2.389 ± 0.369
1.46AspPro: 1.46 ± 0.329
1.194AspGln: 1.194 ± 0.325
2.588AspArg: 2.588 ± 0.467
3.251AspSer: 3.251 ± 0.598
2.654AspThr: 2.654 ± 0.402
4.578AspVal: 4.578 ± 0.498
0.796AspTrp: 0.796 ± 0.258
2.588AspTyr: 2.588 ± 0.444
0.0AspXaa: 0.0 ± 0.0
Glu
5.109GluAla: 5.109 ± 0.546
1.46GluCys: 1.46 ± 0.39
3.384GluAsp: 3.384 ± 0.42
4.644GluGlu: 4.644 ± 0.606
2.322GluPhe: 2.322 ± 0.432
2.72GluGly: 2.72 ± 0.399
0.995GluHis: 0.995 ± 0.314
4.843GluIle: 4.843 ± 0.502
4.578GluLys: 4.578 ± 0.553
5.507GluLeu: 5.507 ± 0.504
2.455GluMet: 2.455 ± 0.424
2.72GluAsn: 2.72 ± 0.378
2.787GluPro: 2.787 ± 0.453
3.715GluGln: 3.715 ± 0.536
3.45GluArg: 3.45 ± 0.477
3.782GluSer: 3.782 ± 0.458
2.189GluThr: 2.189 ± 0.409
3.915GluVal: 3.915 ± 0.539
1.526GluTrp: 1.526 ± 0.298
2.322GluTyr: 2.322 ± 0.369
0.0GluXaa: 0.0 ± 0.0
Phe
1.791PheAla: 1.791 ± 0.402
1.062PheCys: 1.062 ± 0.25
2.588PheAsp: 2.588 ± 0.397
2.588PheGlu: 2.588 ± 0.393
0.995PhePhe: 0.995 ± 0.301
2.654PheGly: 2.654 ± 0.307
0.663PheHis: 0.663 ± 0.21
2.256PheIle: 2.256 ± 0.49
1.924PheLys: 1.924 ± 0.345
1.725PheLeu: 1.725 ± 0.371
1.592PheMet: 1.592 ± 0.343
2.654PheAsn: 2.654 ± 0.368
1.128PhePro: 1.128 ± 0.276
0.929PheGln: 0.929 ± 0.214
1.659PheArg: 1.659 ± 0.355
2.455PheSer: 2.455 ± 0.407
1.924PheThr: 1.924 ± 0.4
1.526PheVal: 1.526 ± 0.374
0.531PheTrp: 0.531 ± 0.162
1.062PheTyr: 1.062 ± 0.289
0.0PheXaa: 0.0 ± 0.0
Gly
4.976GlyAla: 4.976 ± 0.746
1.327GlyCys: 1.327 ± 0.441
4.777GlyAsp: 4.777 ± 0.615
4.711GlyGlu: 4.711 ± 0.553
2.588GlyPhe: 2.588 ± 0.428
5.374GlyGly: 5.374 ± 0.734
0.995GlyHis: 0.995 ± 0.242
4.512GlyIle: 4.512 ± 0.485
6.303GlyLys: 6.303 ± 0.657
5.441GlyLeu: 5.441 ± 0.571
2.057GlyMet: 2.057 ± 0.379
2.919GlyAsn: 2.919 ± 0.357
0.73GlyPro: 0.73 ± 0.209
2.322GlyGln: 2.322 ± 0.439
3.848GlyArg: 3.848 ± 0.431
5.507GlySer: 5.507 ± 0.799
3.782GlyThr: 3.782 ± 0.667
5.905GlyVal: 5.905 ± 0.566
1.393GlyTrp: 1.393 ± 0.363
3.516GlyTyr: 3.516 ± 0.552
0.0GlyXaa: 0.0 ± 0.0
His
1.128HisAla: 1.128 ± 0.29
0.597HisCys: 0.597 ± 0.202
1.062HisAsp: 1.062 ± 0.223
0.995HisGlu: 0.995 ± 0.29
0.663HisPhe: 0.663 ± 0.212
1.858HisGly: 1.858 ± 0.502
0.663HisHis: 0.663 ± 0.219
1.062HisIle: 1.062 ± 0.259
1.261HisLys: 1.261 ± 0.287
1.393HisLeu: 1.393 ± 0.385
0.332HisMet: 0.332 ± 0.163
1.194HisAsn: 1.194 ± 0.232
0.663HisPro: 0.663 ± 0.194
0.796HisGln: 0.796 ± 0.235
1.128HisArg: 1.128 ± 0.323
0.796HisSer: 0.796 ± 0.212
0.73HisThr: 0.73 ± 0.226
1.393HisVal: 1.393 ± 0.315
0.199HisTrp: 0.199 ± 0.11
0.663HisTyr: 0.663 ± 0.217
0.0HisXaa: 0.0 ± 0.0
Ile
6.038IleAla: 6.038 ± 0.599
1.194IleCys: 1.194 ± 0.327
5.175IleAsp: 5.175 ± 0.609
4.512IleGlu: 4.512 ± 0.465
2.057IlePhe: 2.057 ± 0.286
4.644IleGly: 4.644 ± 0.57
1.393IleHis: 1.393 ± 0.297
5.109IleIle: 5.109 ± 0.832
4.246IleLys: 4.246 ± 0.467
3.981IleLeu: 3.981 ± 0.562
1.858IleMet: 1.858 ± 0.345
3.45IleAsn: 3.45 ± 0.453
2.72IlePro: 2.72 ± 0.338
1.592IleGln: 1.592 ± 0.28
3.516IleArg: 3.516 ± 0.525
4.578IleSer: 4.578 ± 0.52
3.981IleThr: 3.981 ± 0.523
4.445IleVal: 4.445 ± 0.459
0.398IleTrp: 0.398 ± 0.133
1.99IleTyr: 1.99 ± 0.437
0.0IleXaa: 0.0 ± 0.0
Lys
5.971LysAla: 5.971 ± 0.631
1.393LysCys: 1.393 ± 0.359
3.516LysAsp: 3.516 ± 0.57
3.317LysGlu: 3.317 ± 0.4
2.322LysPhe: 2.322 ± 0.384
4.047LysGly: 4.047 ± 0.647
1.327LysHis: 1.327 ± 0.34
4.91LysIle: 4.91 ± 0.586
4.18LysLys: 4.18 ± 0.581
4.976LysLeu: 4.976 ± 0.607
2.521LysMet: 2.521 ± 0.395
3.317LysAsn: 3.317 ± 0.614
2.389LysPro: 2.389 ± 0.376
2.787LysGln: 2.787 ± 0.469
3.848LysArg: 3.848 ± 0.601
4.313LysSer: 4.313 ± 0.521
3.516LysThr: 3.516 ± 0.568
4.379LysVal: 4.379 ± 0.551
1.659LysTrp: 1.659 ± 0.377
2.919LysTyr: 2.919 ± 0.509
0.0LysXaa: 0.0 ± 0.0
Leu
5.374LeuAla: 5.374 ± 0.778
1.062LeuCys: 1.062 ± 0.279
3.45LeuAsp: 3.45 ± 0.464
3.981LeuGlu: 3.981 ± 0.552
2.322LeuPhe: 2.322 ± 0.407
4.644LeuGly: 4.644 ± 0.492
1.659LeuHis: 1.659 ± 0.38
4.512LeuIle: 4.512 ± 0.604
4.777LeuLys: 4.777 ± 0.705
4.843LeuLeu: 4.843 ± 0.672
1.526LeuMet: 1.526 ± 0.29
3.848LeuAsn: 3.848 ± 0.498
3.185LeuPro: 3.185 ± 0.605
2.919LeuGln: 2.919 ± 0.433
4.976LeuArg: 4.976 ± 0.447
5.175LeuSer: 5.175 ± 0.563
4.711LeuThr: 4.711 ± 0.55
5.374LeuVal: 5.374 ± 0.572
1.46LeuTrp: 1.46 ± 0.287
2.521LeuTyr: 2.521 ± 0.439
0.0LeuXaa: 0.0 ± 0.0
Met
3.251MetAla: 3.251 ± 0.405
0.265MetCys: 0.265 ± 0.111
1.128MetAsp: 1.128 ± 0.288
1.327MetGlu: 1.327 ± 0.247
0.796MetPhe: 0.796 ± 0.244
1.327MetGly: 1.327 ± 0.303
0.73MetHis: 0.73 ± 0.246
1.725MetIle: 1.725 ± 0.352
2.654MetLys: 2.654 ± 0.492
1.99MetLeu: 1.99 ± 0.347
1.393MetMet: 1.393 ± 0.328
2.057MetAsn: 2.057 ± 0.37
1.791MetPro: 1.791 ± 0.387
1.261MetGln: 1.261 ± 0.305
1.261MetArg: 1.261 ± 0.234
2.654MetSer: 2.654 ± 0.405
1.592MetThr: 1.592 ± 0.345
1.526MetVal: 1.526 ± 0.386
0.597MetTrp: 0.597 ± 0.16
0.863MetTyr: 0.863 ± 0.257
0.0MetXaa: 0.0 ± 0.0
Asn
4.445AsnAla: 4.445 ± 0.527
0.531AsnCys: 0.531 ± 0.193
3.052AsnAsp: 3.052 ± 0.483
2.853AsnGlu: 2.853 ± 0.499
1.194AsnPhe: 1.194 ± 0.283
5.64AsnGly: 5.64 ± 0.629
1.261AsnHis: 1.261 ± 0.287
3.052AsnIle: 3.052 ± 0.434
3.516AsnLys: 3.516 ± 0.47
3.583AsnLeu: 3.583 ± 0.405
1.062AsnMet: 1.062 ± 0.291
2.189AsnAsn: 2.189 ± 0.433
1.194AsnPro: 1.194 ± 0.229
1.46AsnGln: 1.46 ± 0.297
2.455AsnArg: 2.455 ± 0.361
3.516AsnSer: 3.516 ± 0.603
2.455AsnThr: 2.455 ± 0.378
3.052AsnVal: 3.052 ± 0.601
0.663AsnTrp: 0.663 ± 0.219
1.327AsnTyr: 1.327 ± 0.313
0.0AsnXaa: 0.0 ± 0.0
Pro
2.654ProAla: 2.654 ± 0.534
0.597ProCys: 0.597 ± 0.204
2.787ProAsp: 2.787 ± 0.396
2.72ProGlu: 2.72 ± 0.41
1.194ProPhe: 1.194 ± 0.3
1.99ProGly: 1.99 ± 0.421
0.398ProHis: 0.398 ± 0.164
1.725ProIle: 1.725 ± 0.282
1.858ProLys: 1.858 ± 0.334
2.322ProLeu: 2.322 ± 0.406
0.531ProMet: 0.531 ± 0.187
1.327ProAsn: 1.327 ± 0.385
0.929ProPro: 0.929 ± 0.279
1.46ProGln: 1.46 ± 0.346
1.393ProArg: 1.393 ± 0.341
2.521ProSer: 2.521 ± 0.41
1.858ProThr: 1.858 ± 0.34
3.317ProVal: 3.317 ± 0.477
0.531ProTrp: 0.531 ± 0.215
1.327ProTyr: 1.327 ± 0.326
0.0ProXaa: 0.0 ± 0.0
Gln
3.185GlnAla: 3.185 ± 0.725
0.531GlnCys: 0.531 ± 0.187
1.526GlnAsp: 1.526 ± 0.362
2.853GlnGlu: 2.853 ± 0.391
1.393GlnPhe: 1.393 ± 0.279
1.592GlnGly: 1.592 ± 0.427
1.062GlnHis: 1.062 ± 0.316
1.924GlnIle: 1.924 ± 0.457
1.99GlnLys: 1.99 ± 0.344
3.782GlnLeu: 3.782 ± 0.587
1.526GlnMet: 1.526 ± 0.345
1.194GlnAsn: 1.194 ± 0.276
1.858GlnPro: 1.858 ± 0.349
2.72GlnGln: 2.72 ± 0.758
2.322GlnArg: 2.322 ± 0.448
2.787GlnSer: 2.787 ± 0.454
2.256GlnThr: 2.256 ± 0.466
2.455GlnVal: 2.455 ± 0.574
0.464GlnTrp: 0.464 ± 0.137
1.327GlnTyr: 1.327 ± 0.248
0.0GlnXaa: 0.0 ± 0.0
Arg
4.512ArgAla: 4.512 ± 0.548
1.062ArgCys: 1.062 ± 0.351
2.787ArgAsp: 2.787 ± 0.432
3.715ArgGlu: 3.715 ± 0.515
1.791ArgPhe: 1.791 ± 0.4
3.583ArgGly: 3.583 ± 0.463
0.863ArgHis: 0.863 ± 0.188
3.583ArgIle: 3.583 ± 0.417
3.981ArgLys: 3.981 ± 0.508
4.711ArgLeu: 4.711 ± 0.528
1.791ArgMet: 1.791 ± 0.323
3.384ArgAsn: 3.384 ± 0.543
1.261ArgPro: 1.261 ± 0.244
2.389ArgGln: 2.389 ± 0.373
3.118ArgArg: 3.118 ± 0.523
3.384ArgSer: 3.384 ± 0.371
1.924ArgThr: 1.924 ± 0.369
2.853ArgVal: 2.853 ± 0.527
0.663ArgTrp: 0.663 ± 0.204
2.853ArgTyr: 2.853 ± 0.335
0.0ArgXaa: 0.0 ± 0.0
Ser
6.104SerAla: 6.104 ± 0.666
0.597SerCys: 0.597 ± 0.199
4.114SerAsp: 4.114 ± 0.524
3.649SerGlu: 3.649 ± 0.449
2.654SerPhe: 2.654 ± 0.404
6.369SerGly: 6.369 ± 0.87
0.929SerHis: 0.929 ± 0.186
4.18SerIle: 4.18 ± 0.583
3.915SerLys: 3.915 ± 0.561
5.109SerLeu: 5.109 ± 0.628
1.924SerMet: 1.924 ± 0.318
2.853SerAsn: 2.853 ± 0.399
2.654SerPro: 2.654 ± 0.459
2.853SerGln: 2.853 ± 0.439
3.782SerArg: 3.782 ± 0.547
3.583SerSer: 3.583 ± 0.615
4.18SerThr: 4.18 ± 0.622
3.715SerVal: 3.715 ± 0.509
1.128SerTrp: 1.128 ± 0.271
2.123SerTyr: 2.123 ± 0.35
0.0SerXaa: 0.0 ± 0.0
Thr
4.578ThrAla: 4.578 ± 1.122
0.73ThrCys: 0.73 ± 0.267
2.787ThrAsp: 2.787 ± 0.412
4.313ThrGlu: 4.313 ± 0.497
1.526ThrPhe: 1.526 ± 0.396
4.777ThrGly: 4.777 ± 0.677
0.531ThrHis: 0.531 ± 0.176
3.317ThrIle: 3.317 ± 0.536
3.583ThrLys: 3.583 ± 0.59
3.45ThrLeu: 3.45 ± 0.675
1.393ThrMet: 1.393 ± 0.264
2.322ThrAsn: 2.322 ± 0.435
3.251ThrPro: 3.251 ± 0.503
1.99ThrGln: 1.99 ± 0.319
2.521ThrArg: 2.521 ± 0.424
3.118ThrSer: 3.118 ± 0.513
3.848ThrThr: 3.848 ± 0.771
3.052ThrVal: 3.052 ± 0.528
0.597ThrTrp: 0.597 ± 0.271
1.791ThrTyr: 1.791 ± 0.365
0.0ThrXaa: 0.0 ± 0.0
Val
5.507ValAla: 5.507 ± 0.688
0.73ValCys: 0.73 ± 0.225
4.114ValAsp: 4.114 ± 0.474
3.45ValGlu: 3.45 ± 0.543
2.256ValPhe: 2.256 ± 0.452
3.649ValGly: 3.649 ± 0.598
0.995ValHis: 0.995 ± 0.251
6.436ValIle: 6.436 ± 0.741
4.313ValLys: 4.313 ± 0.441
4.246ValLeu: 4.246 ± 0.548
2.389ValMet: 2.389 ± 0.348
4.246ValAsn: 4.246 ± 0.55
1.46ValPro: 1.46 ± 0.279
2.455ValGln: 2.455 ± 0.347
3.583ValArg: 3.583 ± 0.533
4.578ValSer: 4.578 ± 0.519
3.583ValThr: 3.583 ± 0.779
4.379ValVal: 4.379 ± 0.563
1.194ValTrp: 1.194 ± 0.296
2.189ValTyr: 2.189 ± 0.343
0.0ValXaa: 0.0 ± 0.0
Trp
0.863TrpAla: 0.863 ± 0.203
0.398TrpCys: 0.398 ± 0.166
0.796TrpAsp: 0.796 ± 0.221
0.73TrpGlu: 0.73 ± 0.221
0.663TrpPhe: 0.663 ± 0.197
0.597TrpGly: 0.597 ± 0.195
0.663TrpHis: 0.663 ± 0.224
0.863TrpIle: 0.863 ± 0.205
1.194TrpLys: 1.194 ± 0.256
1.659TrpLeu: 1.659 ± 0.382
0.199TrpMet: 0.199 ± 0.12
0.663TrpAsn: 0.663 ± 0.21
0.663TrpPro: 0.663 ± 0.196
1.128TrpGln: 1.128 ± 0.246
1.261TrpArg: 1.261 ± 0.243
0.863TrpSer: 0.863 ± 0.245
1.062TrpThr: 1.062 ± 0.222
1.393TrpVal: 1.393 ± 0.278
0.199TrpTrp: 0.199 ± 0.121
0.464TrpTyr: 0.464 ± 0.156
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.185TyrAla: 3.185 ± 0.431
0.332TyrCys: 0.332 ± 0.119
2.787TyrAsp: 2.787 ± 0.384
1.858TyrGlu: 1.858 ± 0.342
1.46TyrPhe: 1.46 ± 0.268
2.588TyrGly: 2.588 ± 0.412
0.73TyrHis: 0.73 ± 0.225
2.057TyrIle: 2.057 ± 0.394
2.123TyrLys: 2.123 ± 0.439
2.455TyrLeu: 2.455 ± 0.493
0.73TyrMet: 0.73 ± 0.245
1.659TyrAsn: 1.659 ± 0.322
0.995TyrPro: 0.995 ± 0.214
1.46TyrGln: 1.46 ± 0.287
1.526TyrArg: 1.526 ± 0.311
2.919TyrSer: 2.919 ± 0.454
1.99TyrThr: 1.99 ± 0.479
2.986TyrVal: 2.986 ± 0.438
0.663TyrTrp: 0.663 ± 0.202
1.327TyrTyr: 1.327 ± 0.277
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 90 proteins (15073 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski